Exploring the potential mechanism of Lamiophlomis rotata (Benth.) Kudo in treating acne through network pharmacology and experimental verification.

Ethnopharmacological Relevance: Lamiophlomis rotata (Benth.)Kudo(L. rotata), known in China as Duyiwei (DYW), is a well-known traditional Tibetan medicine.

Asparaginase and Autophagy Inhibitors Effectively Remove Senescent Cells by Synergistically Limiting Asparagine Supply.

The accumulation of senescent cells (SNCs) contributes to tissue dysfunction and age-related diseases, creating an urgent need for effective senolytic strategies. We identified a metabolic vulnerability in SNCs characterized by marked downregulation of asparagine synthetase (ASNS), rendering them uniquely dependent on exogenous asparagine (Asn). This vulnerability was exploited through combined treatment with L-asparaginase (ASNase) and autophagy inhibitors, which synergistically deplete Asn via complementary mechanisms: ASNase degrades extracellular Asn pools, while autophagy inhibition blocks intracellular protein recycling as an alternative Asn source.

YOLOv8-Seg with Dynamic Multi-Kernel Learning for Infrared Gas Leak Segmentation: A Weakly Supervised Approach.

Gas leak detection in oil and gas processing facilities is a critical component of the safety production monitoring system. Non-contact detection technology based on infrared imaging has emerged as a vital real-time monitoring method due to its rapid response and extensive coverage. However, existing pixel-level segmentation networks face challenges such as insufficient segmentation accuracy, rough gas edges, and jagged boundaries.

Enhanced protective efficacy of a dendritic cell-targeting truncated F protein delivery via minicircle DNA vaccine against genotype VII newcastle disease virus in chickens.

Newcastle disease (ND), caused by the Newcastle disease virus (NDV), poses a severe threat to global poultry farming, leading to significant losses annually. The limitations of current ND vaccines in terms of efficacy and duration of protection have encouraged the exploration of novel vaccine designs. This study aimed to adopt a delayed-lysis Salmonella strain χ11218 as vaccine delivery vector.

Fabrication of flexibility-rigidity alternately assembled solid-state luminescence covalent organic frameworks: application to rifampicin antibiotics detection and latent fingerprint recognition.

To enhance the fluorescence properties of covalent organic frameworks (COFs) for broader applications, we introduce flexibility-rigidity alternately assembled red fluorescent COFs, named COF-MA. Its uniquely wide layer spacing (7.054 Å) significantly reduces π-π stacking interactions, suppressing fluorescence self-quenching and boosting solid-state emission.

GLUT3 enhances chemosensitivity in glioblastoma by transporting temozolomide and capecitabine.

Glioblastoma multiforme (GBM), the most aggressive brain cancer, is highly resistant to chemotherapy, which profoundly affects patient survival and prognosis. Temozolomide (TMZ), the sole first-line chemotherapeutic agent for GBM, faces substantial challenges in overcoming this resistance. Despite the belief that TMZ is well-absorbed in the small intestine and can effectively cross the blood-brain barrier due to its small molecular size, emerging evidence suggests that its uptake is not merely through passive diffusion across the lipid bilayer but is regulated by Wnt signaling.

The establishment of lipid profiles reference ranges during pregnancy: a systematic review and meta-analysis.

Background: Maternal lipid levels, which are crucial for both foetal development and maternal health, exhibit significant physiological changes during pregnancy. Current reference ranges for lipids that are based on common adults may inadequately assess the appropriate lipid levels during pregnancy and fail to predict potential risks. Therefore, it is necessary to establish trimester-specific reference intervals (TSRIs) for pregnant women during pregnancy.

Dynamic changes in chromatin accessibility within one month of mouse intracerebral hemorrhage.

Intracerebral hemorrhage (ICH) is a severe stroke subtype associated with high mortality and disability. Understanding the molecular and cellular mechanisms behind ICH is vital for developing effective therapeutic interventions. We utilized a single-nucleus assay for transposase-accessible chromatin sequencing (snATAC-seq) to profile changes in chromatin accessibility over the first month following ICH in murine models.

Deep learning assisted non-invasive lymph node burden evaluation and CDK4/6i administration in luminal breast cancer.

Precise lymph node evaluation is fundamental to optimize CDK4/6 inhibitor therapy in luminal breast cancer, particularly given contemporary trends toward axillary surgery de-escalation that may compromise traditional lymph node staging for recurrence risk evaluation. The lymph node prediction network (LNPN) was developed as a multi-modal model incorporating both clinicopathological parameters and ultrasonographic characteristics for lymph node burden differentiation. In a multicenter cohort of 411 patients, LNPN demonstrated robust performance, achieving an AUC of 0.

Construction of an electrochemical sensor based on N-COF@MWCNT-COOH/Tri-AgNPs for sensitive detection of tyrosine in biological fluids.

A sensitive electrochemical sensor was constructed for tyrosine detection by collaborating a highly conductivity, excellent electrocatalysis and large surface of nitrogen-rich porous covalent organic framework in situ grown on three-dimensional conductive carboxylated multi-walled carbon nanotubes network and catalytic activity of triangular silver nanosheets. The prepared electrochemical sensing platform exhibited satisfactory results for detection of tyrosine with a wide linear range of 0.6-280 μM and a low detection limit of 0.

Giant Five-Shell Polyoxometalate Cages and the Single-Cluster-Based Nanowire Superstructures.

The preparation of polyoxometalate molecular cages has always been a long-term and challenging pursuit. In this work, we have successfully prepared two unprecedented giant heterogeneous POM-based purely inorganic molecular cages with symmetry, which contain the aggregation of M (M = Ni, Co)-substituted trilacunary {PW} and Mn-bridged {W}. Sodium ions are used as templates to fill the cages.

Research on the use of a combination of virtual reality with 3D printing technology to address skull base fractures through precision nursing.

Background: The skull base exhibits a complex structure that contains numerous important nerves and blood vessels; furthermore, it is adjacent to important organs in the maxillofacial region. Skull base fractures are relatively common in cases involving traumatic brain injuries (TBI). Most skull base fractures are treated conservatively through absolute bed rest, which entails numerous challenges for nursing care.

A ratiometric fluorescent sensor based on Eu -β-diketone functionalized covalent organic framework hybrid material-loaded in electrospun polyvinylidene fluoride film for ultra-sensitive and rapid detection of glyphosate.

The extensive application of glyphosate (Gly) presents significant environmental and health risks, underscoring the necessity for advanced sensors to monitor Gly levels. In this study, a novel ratiometric fluorescent sensor (Eu-TTA@COOH-COF) was developed by coordinating Eu³ ⁺ ions with carboxylated covalent organic frameworks (COOH-COF) and grafting 2-thenoyltrifluoroacetone (TTA) ligand to enhance Eu³ ⁺ sensitization. Gly detection relied on its energy competition with the COF, which quenched the characteristic red emission of Eu³ ⁺ (613 nm), and electron transfer to the COOH-COF matrix amplifying the intrinsic blue fluorescence emission (435 nm) of the COF.

An artificial neural network model based on CDs@MOF/COF composites for the ultra-sensitive fluorescence detection of glyphosate.

Glyphosate (Gly) overuse threatens public health and ecosystems, necessitating highly sensitive detection. This work developed a novel ratiometric fluorescent probe, CDs@Cu-MOF/TMTA-COF, by integrating carbon dots (CDs), metal organic frameworks (MOFs), and covalent organic frameworks (COFs). We encapsulated CDs within Cu-MOF in-situ grown onto sp‑carbon conjugated TMTA-COF.

Lactate dehydrogenase A: a potential new target for tumor drug resistance intervention.

Tumor drug resistance is a crucial scientific and technological issue that urgently needs to be addressed in cancer treatment. In recent years, research on Lactate dehydrogenase A (LDHA) in the field of tumor drug resistance has progressively deepened, with breakthrough advancements achieved. Due to their metabolic characteristics, tumors often exhibit abnormal LDHA expression, which meets their growth requirements by promoting glycolysis and lactate production.

A Drosophila single-cell 3D spatiotemporal multi-omics atlas unveils panoramic key regulators of cell-type differentiation.

The development of a multicellular organism is a highly intricate process tightly regulated by numerous genes and pathways in both spatial and temporal manners. Here, we present Flysta3D-v2, a comprehensive multi-omics atlas of the model organism Drosophila spanning its developmental lifespan from embryo to pupa. Our datasets encompass 3D single-cell spatial transcriptomic, single-cell transcriptomic, and single-cell chromatin accessibility information.

AI-Driven Transcriptome Prediction in Human Pathology: From Molecular Insights to Clinical Applications.

Gene expression regulation underpins cellular function and disease progression, yet its complexity and the limitations of conventional detection methods hinder clinical translation. In this review, we define "predict" as the AI-driven inference of gene expression levels and regulatory mechanisms from non-invasive multimodal data (e.g.

Comparison of the diagnostic capabilities of tNGS and mNGS for pathogens causing lower respiratory tract infections: a prospective observational study.

Aims: Pathogens in lower respiratory tract infections(LRTI) are complex. Conventional microbiological testings(CMTs) are time-consuming and inaccurate. mNGS is widely used to overcome these issues.

Malignant transformation of pituitary adenoma to carcinosarcoma: illustrative case.

Background: Carcinosarcomatous transformation is a rare complication following radiotherapy for pituitary adenomas (PAs).

Observations: The authors report what they believe to be the first case of a benign nonfunctioning PA developing into a carcinosarcoma after surgery and radiotherapy.

Lessons: Radiotherapy for PAs should account for the risk of secondary intracranial tumors, with long-term follow-up recommended for treated patients.

Improved protection against H9N2 avian influenza virus challenge in chickens by the presence of LTB adjuvant on the 3M2e-NP nanoparticle vaccine delivered by sifA deficient Salmonella.

The H9N2 avian influenza virus is a still a great threat to poultry production and public health due to its variation. The highly conserved extracellular domain, M2 ion channel (M2e), and the nuclear protein (NP) are usually considered to be potential targets for a broad-spectrum influenza vaccine. In this study, we took use of a regulated delayed lysis Salmonella χYL56 lacking the sifA gene as a delivery vector to deliver a lumazine synthase (LS) based nanoparticle vaccine decorating with three copies of M2e (H9N2, H5N1 and H1N1 subtype, 3M2e) and NP protein, without or with LTB adjuvant, yielding S311 and S325, respectively.

Structural characterization of a fucoidan from Sargassum fusiforme and its antagonistic effect on T cell proliferation-inhibition induced by CAFs.

A water-soluble sulfated polysaccharide, SFAP-4, was isolated from Sargassum fusiforme via ion-exchange and gel-filtration column chromatography. It comprised a large amount of fucose (Fuc), minor amount of galactose (Gal), and small amounts of glucose (Glc), mannose (Man), rhammnose (Rha), xylose (Xyl) and glucuronic acid (GlcA). Structural analysis showed that SFAP-4 has very complex structure.

O-GalNAc Glycosylation Activates MBL-Mediated Complement and Coagulation Cascades to Drive Organotropic Metastasis.

Liver metastasis is prevalent among patients with neuroendocrine prostate cancer (NEPC) and other types of neuroendocrine (NE) cancers, featuring with an aggressive clinical course and a dismal prognosis. However, the cellular and molecular mechanisms underlying liver-specific metastatic tropism in NE cancers remain poorly understood. Intriguingly, it is found that NEPC liver metastatic foci are frequently associated with thrombi.

Depot-Specific Changes in Gene Expression in the Inguinal and Epididymal Adipose Tissues in Response to the High-Intensity Interval Training and Moderate-Intensity Continuous Training in Mice.

Different exercise modes have different mechanisms of impact on white adipose tissue (WAT) and metabolic health. However, specific effects of particular exercise modalities on different adipose tissue depots remain unclear. This study aimed to compare the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on gene expression in the inguinal (subcutaneous) and epididymal (visceral) WAT in mice.

Asymmetric interfacial Ru single-atom sites and nanoparticles for promoting the hydrogen evolution reaction in a wide pH range.

Herein, the achieved asymmetric interfacial Ru single-atom sites and nanoparticles in Ru/NPC through the coordination-pyrolysis strategy exhibit remarkable HER activities with small overpotential and low Tafel slope in a wide pH range.