Exploring the potential mechanism of Lamiophlomis rotata (Benth.) Kudo in treating acne through network pharmacology and experimental verification.

Ethnopharmacological Relevance: Lamiophlomis rotata (Benth.)Kudo(L. rotata), known in China as Duyiwei (DYW), is a well-known traditional Tibetan medicine.

A cascade amplification platform integrating entropy-driven DNA nanomachine with CRISPR/Cas12a for microRNA-21 and Listeria monocytogenes detection.

As one of enzyme-free amplification strategies, entropy-driven catalytic (EDC) based on toehold-mediated strand displacement reaction could achieve efficient amplification without cumbersome temperature changing and expensive enzymes, which shows great potential in biological sensing. However, the limitations in reaction velocity and sensitivity need to be further improved. Herein, a cascade platform integrating entropy-driven DNA nanomachine with CRISPR/Cas12a was proposed.

variants drive chromosomal fission and accelerate speciation in zokors.

Chromosomal fissions and fusions are common, yet the molecular mechanisms and implications in speciation remain poorly understood. Here, we confirm a fission event in one zokor species through multiple-omics and functional analyses. We traced this event to a mutation in a splicing enhancer of the DNA repair gene in the fission-bearing species, which caused exon skipping and produced a truncated protein that disrupted DNA repair.

Effects of red clover isoflavone on lactation performance, milk quality and nitrogen metabolism in dairy cows.

Reducing nitrogen loss and improving nitrogen utilization efficiency in dairy cows can reduce economic costs and mitigate the environmental impact of nitrogen emissions. Red clover isoflavone, a natural compound derived from plant extract, has the advantage of biological safety. This study aimed to investigate the effects of red clover isoflavone on lactation performance and nitrogen metabolism in dairy cows.

Molecular Epidemiology and Genomic Insights into Enterobacter cloacae Complex Co-Harboring bla and mcr: Implications for Resistance Gene Transmission.

Objective: This study aims to systematically investigate the molecular epidemiology and genomic characteristics of Enterobacter cloacae complex (ECC) strains globally harboring bla and mcr, as well as the co-existence of drug resistance genes. The goal is to provide insights and recommendations for monitoring clinical drug-resistant strains and super-resistant plasmids.

Methods: This study analyzed 281 ECC isolates harboring both bla and mcr, obtained from NCBI GenBank database (2003-2024) with whole-genome sequencing data.

Oxidative-Inflammatory Crosstalk and Multi-Target Natural Agents: Decoding Diabetic Vascular Complications.

Diabetes mellitus (DM) is one of the leading causes of death and disability worldwide and its prevalence continues to rise. Chronic hyperglycemia exposes patients to severe complications. Among these, diabetic vascular lesions are the most destructive.

Codon Usage Pattern of Papillomavirus E6, E7, and L1 Genes Across Hosts.

This study investigates the codon usage preferences of papillomaviruses across different hosts and examines the evolutionary relationships of key genes (L1, E6, and E7). Complete genome sequences of 86 papillomavirus strains were retrieved from the PaVE database. Phylogenetic trees were constructed using MAFFT and ITOL, and codon usage indices, including RSCU, ENC, CAI, and GC3, were calculated using CodonW.

A Multimodal MRI-Based Model for Colorectal Liver Metastasis Prediction: Integrating Radiomics, Deep Learning, and Clinical Features with SHAP Interpretation.

: Predicting colorectal cancer liver metastasis (CRLM) is essential for prognostic assessment. This study aims to develop and validate an interpretable multimodal machine learning framework based on multiparametric MRI for predicting CRLM, and to enhance the clinical interpretability of the model through SHapley Additive exPlanations (SHAP) analysis and deep learning visualization. : This multicenter retrospective study included 463 patients with pathologically confirmed colorectal cancer from two institutions, divided into training (n = 256), internal testing (n = 111), and external validation (n = 96) sets.

Evolution, dissemination, and genetic dynamics of the carbapenem resistance gene in China.

Background: , which encodes a metallo-β-lactamase that can hydrolyze most β-lactam antibiotics, has become a serious public health concern in China. It is crucial to investigate the evolution, dissemination, and genetic dynamics of to develop potential strategies to control the proliferation of .

Methods: In this study, we collected 1021 -positive isolates, which features 67 new genomes from our laboratory and 954 genomes from NCBI.

Animal studies and AI-based analysis identify the efficacy of berberine to inhibit experimental abdominal aortic aneurysms.

Background: Currently, no drug treatment is available for abdominal aortic aneurysms (AAAs). Berberine, an alkaloid extracted from the traditional Chinese herbs Coptis chinensis and Phellodendron amurense, has several beneficial biological effects, including anti-inflammatory properties.

Purpose: The aim of this study was to explore whether berberine affects the pathogenesis of AAAs.

Genetic characteristics of rotavirus a in Shenzhen and Zhuhai, China, 2020-2023.

Background: Rotavirus A group (RVA) is a leading cause of viral diarrhea, posing a substantial economic and public health burden. Compared to other enteric viruses, RVA possesses diverse genetic mechanisms, making it more challenging to control and prevent. Moreover, surveillance and evolutionary studies on RVA remain limited in Southern China.

[Decoupling Effect and Interactive Relationship Among Transportation Infrastructure, Economic Growth, and Carbon Emissions in China].

The construction of transportation infrastructure boosts economic growth while facing the challenge of carbon emissions pressure. Clarifying the relationship among transportation infrastructure, economic growth, and carbon emissions is important in order to promote the realization of the goal of "dual-carbon." Based on the panel data of 30 provinces in China from 2002 to 2021, the research period was divided into four stages （2002-2006, 2007-2011, 2012-2016, and 2017-2021）.

A Multi-Omics Integration Framework with Automated Machine Learning Identifies Peripheral Immune-Coagulation Biomarkers for Schizophrenia Risk Stratification.

Schizophrenia (SCZ) is a complex psychiatric disorder with heterogeneous molecular underpinnings that remain poorly resolved by conventional single-omics approaches, limiting biomarker discovery and mechanistic insights. To address this gap, we applied an artificial intelligence (AI)-driven multi-omics framework to an open access dataset comprising plasma proteomics, post-translational modifications (PTMs), and metabolomics to systematically dissect SCZ pathophysiology. In a cohort of 104 individuals, comparative analysis of 17 machine learning models revealed that multi-omics integration significantly enhanced classification performance, reaching a maximum AUC of 0.

Role of Plasma-Derived Exosomal MicroRNAs in Mediating Type 2 Diabetes Remission.

This study aimed to identify plasma exosomal microRNAs (miRNAs) associated with weight loss and type 2 diabetes (T2D) remission following low-calorie diet (LCD) intervention. A 6-month dietary intervention targeting T2D remission was conducted among individuals with T2D. Participants underwent a 3-month intensive weight loss phase consuming LCD (815-835 kcal/day) and a 3-month weight maintenance phase (N = 32).

Repeatability and reproducibility of anterior segment measurements with a new full-range SS-OCT and comparison with an AS-OCT.

Purpose: To evaluate the intraobserver repeatability and interobserver reproducibility of anterior segment parameters measured by the new YG-100K full-range swept-source optical coherence tomographer (SS-OCT) and compare them with values from the validated CASIA 2 anterior segment optical coherence tomographer (AS-OCT).

Methods: In a randomized sequence, the first operator conducted three consecutive measurements with both devices, while a second operator independently conducted three consecutive measurements with only the YG-100K. Each device's built-in software automatically identified the scleral spur and angle recess.

Distribution characteristics and influencing factors of overweight and obesity among urban and rural primary and secondary school students in Hunan Province.

Objectives: The prevalence of overweight and obesity among children and adolescents continues to rise, becoming one of the most serious global public health issues of the 21st century. Given the differing growth and development environments between urban and rural children, associated risk factors also vary. This study aims to explore the distribution characteristics and influencing factors of overweight and obesity among urban and rural primary and secondary school students in Hunan Province, providing scientific evidence for targeted interventions.

Elevated CO enhances Pb availability and desorption kinetics at the root-soil interface: Role of dissolved organic matter in soil solution.

Understanding the ecological forces driving lead (Pb) translocation, distribution, and accumulation in plant‒soil ecosystems under elevated CO (eCO) is essential for optimizing phytoremediation strategies and predicting Pb cycling dynamics. This study systematically investigated Pb availability and desorption kinetics in a root-soil ecosystem with the metal-tolerant plant Trifolium repens L., using diffusive gradients in thin films (DGTs), fluorescence spectroscopy, and kinetic modelling.

Host genomic convergence, rather than gut microbiome convergence, underlies the convergent evolution of subterranean adaptation in mammals.

Subterranean mammals exhibit diverse traits for underground life, making them ideal models for studying molecular mechanisms of adaptation and convergence in extreme environments. To investigate these processes at both the genomic and gut microbiome levels, we assemble a chromosome-level genome of the Chinese bamboo rat (Rhizomys sinensis) and identify an expansion of the Hsp90 gene family, potentially associated with hypoxia adaptation. Transcriptomic analysis reveals gene expression changes related to tooth development and visual perception in the Chinese bamboo rat.

Assessing the economic burden and health-related quality of life in chinese patients with fibrodysplasia ossificans progressiva: a questionnaire survey analysis.

Background: Fibrodysplasia ossificans progressiva (FOP) is an ultra-rare, disabling genetic disorder condition that lacks real-world evidence on disease burden. This study aims to investigate the basic characteristics, diagnostic status, prognosis, economic burden and quality of life of FOP patients in China through self-reported data .

Methods: An online survey was conducted through the "China Cloud Platform for Rare Diseases" among patients recruited from the FOP patient organization in March 2023.

Incidence of Vaginal Intraepithelial Neoplasia After Hysterectomy for Cervical Cancer or Cervical Intraepithelial Neoplasia: A Systematic Review and Meta-analysis.

Objective: To investigate the incidence of vaginal intraepithelial neoplasia (VAIN) 1+ in patients after hysterectomy for cervical intraepithelial neoplasia (CIN) or cervical cancer.

Data Sources: A systematic search was conducted in PubMed, EMBASE, and the Cochrane Central Register of Controlled Trials from inception to January 2025.

Methods Of Study Selection: We identified 7,234 studies, 48 of which were included in the meta-analysis.

Associations of four important dietary pattern scores, micronutrients with sarcopenia and osteopenia in adults: results from the National Health and Nutrition Examination Survey.

Introduction: We aimed to comprehensively evaluate the association between four important dietary patterns and micronutrients with sarcopenia and osteopenia. Data were obtained from the National Health and Nutrition Examination Survey 2005-2018.

Methods: The association between dietary patterns and micronutrients with sarcopenia and osteopenia was evaluated by logistic regression models.

Urate-lowering and renal-protective effects of sugarcane polyphenols in hyperuricemia: mechanisms and key components.

Hyperuricemia (HUA) is a metabolic disorder characterized by abnormally elevated levels of uric acid (UA) in the blood, often accompanied by renal damage. The continuous rise in HUA incidence necessitates the development of safe, efficient natural urate-lowering pathways. This study combined network pharmacology prediction with rat model experiments to systematically evaluate the urate-lowering potential of sugarcane polyphenols (SP), a byproduct of the sugarcane sugar industry.

Lirispirolides A-L, a new class of sesquiterpene-monoterpene heterodimers with anti-neuroinflammatory activity from the rare medicinal plant Liriodendron chinense.

Lirispirolides A-L (1-12), twelve novel sesquiterpene-monoterpene heterodimers featuring distinctive carbon skeletons, were isolated from the branches and leaves of Chinese tulip tree [Liriodendron chinense (L. chinense)], a rare medicinal and ornamental plant endemic to China. The structural elucidation was accomplished through comprehensive spectroscopic analyses, quantum-chemical calculations, and X-ray crystallography.

"Strong Assisting Weak" Effect for Long-Lived Room Temperature Phosphorescence in Host-Guest-Doped Systems.

Organic room temperature phosphorescence (RTP) materials, with long lifetime, large Stokes shift, and high signal-to-noise ratio, have great potential in numerous fields. And now, the host-guest-doped systems have become an effective approach for facilitating RTP, regardless of the still unclear inherent mechanism. In this work, a series of host-guest-doped RTP materials were constructed, and based on experimental results, the "strong assisting weak" effect was proposed.

3D-Printed Bioinspired Meta-Structural Perovskite Catalysts for Dry Reforming of CH and CO.

Conventional packed-bed catalysts suffer from single-scale porosity, insufficient mechanical strength, and suboptimal mass transfer efficiency. Inspired by the fractal structure of the lung bronchi, a design and 3D printing method for gradient meta-structural catalysts is proposed by integrating synthesized LaFeNiO (LFN) perovskite with pseudo-boehmite, achieving ultralow pressure drop and high catalytic efficiency. Computational fluid dynamics and reaction simulations guide the design of uniform and gradient-structured catalysts with hierarchical woodpile channels (0.