From mung bean to Minglv-flavor baijiu: Unraveling the signature aroma profile and aging dynamic.

Minglv-flavor (MLF) Baijiu, brewed with mung beans, exhibits a characteristic aroma profile. However, key aroma compounds and dynamic evolution during aging have not been systematically investigated. Through recombination and omission experiments, 21 key aroma compounds were confirmed, including ethyl hexanoate, ethyl butyrate, ethyl pentanoate, ethyl octanoate, ethyl 3-methylbutanoate, ethyl 2-methylpropanoate, ethyl 2-methylbutanoate, hexanoic acid and pentanoic acid, which synergistically constituted the aroma profile of MLF Baijiu, characterized by a prominent mung bean aroma accompanied by harmonious fruity and floral notes.

Genetic variants affecting RNA stability influence complex traits and disease risk.

Gene expression is modulated jointly by transcriptional regulation and messenger RNA stability, yet the latter is often overlooked in studies on genetic variants. Here, leveraging metabolic labeling data (Bru/BruChase-seq) and a new computational pipeline, RNAtracker, we categorize genes as allele-specific RNA stability (asRS) or allele-specific RNA transcription events. We identify more than 5,000 asRS variants among 665 genes across a panel of 11 human cell lines.

Interictal Epileptiform Discharge Detection using Dual-domain Features and GAN.

Interictal Epileptiform Discharge is essential for identifying epilepsy. However, the unpredictable and non-stationary nature of electroencephalogram (EEG) patterns poses considerable challenges for reliable identification. Manual interpretation of EEG is subjective and time-consuming.

Short-course PD-1 blockade in locally advanced nasopharyngeal carcinoma: a phase II randomized trial protocol (Tori-013).

While immunotherapy has demonstrated encouraging efficacy in locally advanced nasopharyngeal carcinoma (LANPC), the optimal combination modalities and treatment duration remain undetermined. In the present study, we developed a clinical trial protocol to evaluate shortened period of immunotherapy could enhance the efficacy of LANPC. This open-label, randomized, single-blind, multicenter phase II trial (Tori-013) investigates the efficacy and safety of toripalimab (anti-PD-1 monoclonal antibody) combined with induction chemotherapy (IC) followed by concurrent chemoradiotherapy (CCRT) in patients with stage III/IVa nasopharyngeal carcinoma (NPC).

Inter-relationships of Depressive and Insomnia Symptoms With Health Satisfaction in Heart Disease and Heart Disease-Free Older Adult Samples: Findings From a United States National Survey.

Background: Older adults with heart disease experience higher rates of depression and insomnia compared with heart disease-free peers. Aside from these psychological disturbances, overall health satisfaction, as a key indicator of subjective health status, may be affected by heart disease status. In spite of these overall associations, symptom-level relationships between depression, insomnia, and health satisfaction remain unclear.

Risk factors for severe COVID-19 and development of a predictive model.

A clinical case‒control study was conducted to identify risk factors for severe COVID-19 and to develop a predictive risk model to provide a reference for the dynamic assessment of the severity of disease in COVID-19 patients. A total of 410 patients with COVID-19 were included in the study, of whom 132 had severe or critical cases. The clinical data of the patients were collected, and the variables were subsequently screened via LASSO regression analysis and 10-fold cross-validation.

Integrated transcriptomic and metabolic analysis reveal the mechanism controlling cucumber-pumpkin grafting compatibility.

Background: The most widely used rootstock of cucumber grafting is pumpkin (Cucurbita moschata). The main task of pumpkin rootstock breeding is to evaluate its compatibility with cucumber scions. One of the main factors affecting grafting compatibility is the rootstock bleeding sap, which affects the wound healing of graft junction.

Advancements in nanomedicine for modulating ischemic cardiomyopathy therapy.

Ischemic cardiomyopathy receives significant attention due to its high mortality and morbidity worldwide. Numerous research has revealed that blood supply, ROS, inflammation, and thrombus play pivotal roles in the progression of myocardial damage and remodeling. The central approach for managing ischemic cardiomyopathy is the administration of therapeutic drugs against the pathological mechanism.

Research on the U-shaped relationship between marketization process and corporate capital structure: Evidence from Chinese listed companies.

This paper explores the U-shaped relationship between marketization and corporate capital structure using panel data from Chinese listed firms (2012-2021). Applying fixed effects and nonlinear regressions, we find that as marketization increases, leverage first declines and then rises, confirming a robust U-shaped pattern. In early reforms, improved institutions reduce debt reliance; in advanced stages, enhanced capital markets facilitate debt access.

Uniform spherical chiral polyrotaxanes with steerable circularly polarized luminescence.

Spherical chiral polyrotaxanes (CPRs) with steerable circularly polarized luminescence (CPL) are intriguing as advanced chiroptical materials but have not been reported to date. Here, we constructed spherical chiral polyrotaxane (CPR) materials with steerable CPL by utilizing cyclodextrins (α-, β-, or γ-CyD) as the chiral wheel. Importantly, the structure and the CPL performance of the CPR materials can be regulated by varying the feeding amount and the type of cyclodextrin (α-, β-, or γ-CyD).

Clinical characteristics, pregnancy outcomes, and neonatal effects of cytomegalovirus infection in pregnant women in Liuzhou city, North central Guangxi of Southern China: a retrospective study from 2018 to 2024.

Objective: To evaluate the clinical characteristics, pregnancy and neonatal outcomes of cytomegalovirus (CMV) infection in pregnant women.

Methods: This retrospective study included 22,673 pregnant women from Liuzhou, Guangxi, China, between 2018 and 2024. Amniotic fluid samples collected during mid-to-late pregnancy were tested for CMV DNA.

Edge computing with federated learning for early detection of citric acid overdose and adjustment of regional citrate anticoagulation.

Regional citrate anticoagulation (RCA) is critical for extracorporeal anticoagulation in continuous renal replacement therapy done at the bedside. To make patients' data more secure and to help with computer-based monitoring of dosages, we suggest a system that uses machine learning. This system will give early alerts about citric acid overdose and advise changes to how much citrate and calcium gluconate are infused into the patient's body.

The Impact of Artificial Water Diversion-induced Algal Blooms on Carbon Balance in a Semi-enclosed Bay.

Artificial water diversion is widely used to address water security; yet, its impacts on phytoplankton communities and coastal carbon balance remain poorly understood. Using a seasonal diversion project in a semi-enclosed bay as a case study, we analyzed phytoplankton composition via morphological methods and assessed carbon balance through simultaneous measurements of primary production (P), ecosystem respiration rate (R), and production-to-respiration (PP/R) ratio. Our results showed that artificial water diversion activities during the wet month enhanced hydrological connectivity and phytoplankton homogeneity, triggering a mixed diatom-dinoflagellate bloom.

Purification, Composition, and Anti-Inflammatory Activity of Polyphenols from Sweet Potato Stems and Leaves.

Sweet potato stems and leaves (SPSL) are rich in bioactive polyphenols, yet their utilization remains underexplored. This study established an efficient method for SPSL polyphenol enrichment using macroporous resins, with UHPLC-QE-MS/MS characterization of the purified polyphenols (PP) and subsequent evaluation of anti-inflammatory activity. The results showed that NKA-II resin demonstrated the best purification effect on SPSL polyphenols among the six tested resins.

A Novel Lytic Phage Harboring an Unprecedented Tail-Protein Domain Combination Capable of Lysing Cross-Host-Transmitted Strains.

The emergence of multidrug-resistant poses a significant threat to global public health and food safety, necessitating the urgent search for new strategies to replace conventional antibiotics. Phages are viruses that can directly target bacteria and have garnered attention in recent years for their development as antibiotic alternatives. In this study, 4458 samples were collected from farms, supermarkets, and human feces, yielding 65 strains of , which were serotyped using multiplex PCR.

Transformative metal-ligand nanocoating for dual antibacterial and antithrombotic functionality in hemodialysis catheters.

Hemodialysis catheters are life-saving for end-stage renal disease patients but suffer from catheter-related bloodstream infections and thrombosis, which impair device function and threaten patient survival. Although functional coatings have been employed to mitigate some of these complications, the development of coatings that simultaneously exhibit both antibacterial and antithrombotic properties remains a significant challenge. To address these issues, we developed a PDA/Hep/Cu nanocomposite coating through sequential metal-ligand coordination, polymerization, and covalent immobilization.

Reducing PM2.5 Exposure Lowers Dyslipidemia Risk: A Longitudinal Quasi-Experimental Study.

Evidence demonstrating the beneficial effects of improved air quality on lipid health is scarce. This study addresses this gap by examining whether reducing PM2.5 exposure can decrease the risk of dyslipidemia.

Intervention effect of nursing techniques of traditional Chinese medicine on post-stroke insomnia: a network meta-analysis.

Background: Insomnia is a common problem after a stroke, affecting daily life and the recovery process. Among the many Traditional Chinese Medicine(TCM) nursing techniques, it is still unknown which measures are most effective.

Objective: To evaluate the efficacy of different TCM nursing techniques on post-stroke insomnia(PSI) by network meta-analysis(NMA).

Saline flushing to prevent circuit clotting during CRRT without anticoagulant: a randomized controlled study.

Background: Regional citrate anticoagulation (RCA) is the preferred strategy during continuous renal replacement therapy (CRRT). However, saline flushing is often used when anticoagulants are contraindicated, although its effectiveness remains uncertain. This study evaluated the efficacy of different saline flushing strategies in preventing circuit clotting during anticoagulant-free CRRT in critically ill patients.

Degradation of bisphenol A during heat-activated peroxodisulfate treatment: kinetics, mechanism, and transformation products.

In this study, we systematically investigated the degradation kinetics, mechanism, and transformation products of bisphenol A (BPA) in a heat-activated peroxodisulfate (heat/PDS) system. The pseudo-first-order rate constants ( ) values of BPA increased with initial PDS dosage (0-1.0 mM), temperature (50-65 °C), and solution pH (4.

Evolution, dissemination, and genetic dynamics of the carbapenem resistance gene in China.

Background: , which encodes a metallo-β-lactamase that can hydrolyze most β-lactam antibiotics, has become a serious public health concern in China. It is crucial to investigate the evolution, dissemination, and genetic dynamics of to develop potential strategies to control the proliferation of .

Methods: In this study, we collected 1021 -positive isolates, which features 67 new genomes from our laboratory and 954 genomes from NCBI.

Aberrant PJA2-CHRM3 signaling creates a therapeutic vulnerability in gastric tumor.

Background: Vagal innervation plays a pivotal role in gastric tumorigenesis and tumor progression. However, the upstream signaling regulating acetylcholine receptors (AChRs) and its contribution to carcinogenesis remains largely elusive.

Methods: We constructed Trp53; Cdh1 mouse gastric organoids to recapitulate the morphological and functional characteristics of diffuse-type gastric cancer (DGC) for FDA-approved drug (1464 compounds) screening.

Effects of different blood flow rates on filter and circuit life in non-anticoagulation CRRT: Protocol for a three-arm, single-blind randomized controlled trial (the Flow-CRRT Study).

Background: Non-anticoagulation is a commonly used strategy in continuous renal replacement therapy (CRRT) among patients with high-bleeding risk. However, the optimal blood flow rate (BFR) to maximize filter and circuit life remains uncertain. This study is designed to elucidate the impact of different BFRs on the durability of filters and circuits in CRRT without anticoagulation.

Autonomous microfluidic influenza A/B subtyping system using on-chip multiplex isothermal amplification for field-deployable surveillance.

Influenza viruses present significant challenges to global health. A rapid recombinase polymerase amplification (RPA)-based detection system for multiple influenza strains (A, H1N1/H2N2/H3N2/H5N1/H7N9 and B) has been developed to address the limitations of 2-h Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) tests. By using optimized primers targeting key viral proteins (M, NA, HA, PA), the method achieves detection in 10 min with 0.

Phosphatidylethanolamine/PKA signal axis mediated human milk extracellular vesicles enhance adipose thermogenesis and lipolysis via mitochondrial remodeling.

Background/objectives: Emerging evidence suggests that extracellular vesicles (EVs) in breast milk may serve as an abundant source of candidates for regulating metabolism. Our study aims to identify phospholipids derived from human milk EVs (mEVs) that can benefit people with obesity.

Methods: Infant mice were either fed with formula milk or breastfed to investigate whether mEVs affect adipose tissue physiology in infants.