Targeted delivery and pro-apoptotic efficacy of an Epstein-Barr virus nuclear antigen 1-specific affibody in EBV-infected cells in vitro.

Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) sustains viral latency and drives oncogenesis in EBV-driven malignancies such as nasopharyngeal carcinoma and lymphomas. The dimerization of EBNA1 acts as an indispensable molecular switch governing EBV latency and oncogenesis. Disruption of EBNA1 dimerization is a promising strategy, but existing small-molecule inhibitors lack sufficient specificity.

Dingxin Recipe III Ameliorates Endothelial Cell Senescence in Atherosclerosis by Activating the FOXO3a/Pink1/Parkin Axis.

Ethnopharmacological Relevance: Dingxin Recipe III (DXRIII) is a traditional Chinese medicinal formulation that has been employed in clinics for over two decades. It is utilized in the treatment of cardiovascular diseases associated with atherosclerosis (AS) through mechanisms purported to involve the clearing of heat and detoxification, as well as the promotion of blood circulation and the removal of blood stasis. Despite its widespread application and reported therapeutic benefits, its exact mechanisms remain incompletely elucidated.

PSMA-1-DOTA Potentially for Effective Targeted Radioligand Therapy of Prostate Cancer.

Purpose: While PSMA-targeted radioligand therapy (RLT) has shown remarkable efficacy for treating end-stage prostate cancer, the α-emitting RLT often results in severe salivary gland toxicity, limiting its use. Various strategies to mitigate this side effect have been attempted with limited success. Accordingly, this study introduced a new PSMA-targeting ligand with more favorable binding characteristics than the existing ligands.

Clinical characteristics, pregnancy outcomes, and neonatal effects of cytomegalovirus infection in pregnant women in Liuzhou city, North central Guangxi of Southern China: a retrospective study from 2018 to 2024.

Objective: To evaluate the clinical characteristics, pregnancy and neonatal outcomes of cytomegalovirus (CMV) infection in pregnant women.

Methods: This retrospective study included 22,673 pregnant women from Liuzhou, Guangxi, China, between 2018 and 2024. Amniotic fluid samples collected during mid-to-late pregnancy were tested for CMV DNA.

Maximized atom utilization in a high-entropy metallene via single atom alloying for boosted nitrate electroreduction to ammonia.

High-entropy alloys, with their unique structural characteristics and intrinsic properties, have evolved to be one of the most popular catalysts for energy-related applications. However, the geometry of the traditional nanoparticle morphology confines the majority of active atoms to the particle core, deeming them ineffective. In this study, we present a class of two-dimensional high-entropy alloys, namely, high-entropy metallenes, constructed by alloying various single-atom metals in atomically thin layers and reveal their great feasibility for electrocatalytic nitrate reduction to ammonia.

Fucoxanthin protects retinal ganglion cells and regulates Parkin-mediated mitophagy in experimental glaucoma.

Objective: Glaucoma is a common neurodegenerative disease resulting in irreversible blindness. This study investigates whether fucoxanthin can safeguard retinal ganglion cells (RGCs) by modulating Parkin-mediated mitophagy in experimental glaucoma.

Methods: An experimental glaucomatous model was induced in Sprague-Dawley rats via translimbal laser photocoagulation.

Anesthesia management for lung transplant in children with obliterated bronchiolitis after hematopoietic stem cell transplantation: a single-center experience.

Background: Lung transplantation (LTx) is the definitive treatment for patients with end-stage lung diseases, but its application in pediatrics is limited by donor scarcity and surgical complexity. There is a general lack of experience and consensus on anesthetic management during LTx in children with bronchiolitis obliterans syndrome (BOS). We summarized the data of children undergoing LTx with BOS after hematopoietic stem cell transplantation (HSCT) at The Second Affiliated Hospital of Zhejiang University School of Medicine (SAHZU) to devise an anesthetic management strategy and describe practical experience.

The Scarlet Alchemy of Survival: Integrated Transcriptomic and Metabolomic Analysis of Leaf Coloration in Endangered .

is a rare and endangered deciduous tree native to China, valued for its vibrant autumn foliage and ornamental appeal. Its leaves exhibit striking coloration, ranging from red to yellow and purple, yet the physiological and molecular mechanisms behind this variation remain poorly understood. Here, we combined transcriptomic, metabolomic, and physiological analyses to investigate pigment changes within the yellow leaf phenotype of .

Development of Z- granzyme B immunoaffitoxin: dual mechanisms targeting hpv16-positive cervical cancer through epithelial-mesenchymal transition inhibition and cell death.

Introduction: Cervical cancer, predominantly caused by high-risk human papillomavirus (HPV), remains a major global health challenge. HPV16 is the most prevalent type, and its oncoprotein E7 promotes epithelial-mesenchymal transition (EMT), a critical step in metastasis. Current therapies for HPV16-related cancers are often insufficient, highlighting the need for targeted treatments.

Natural yam pigment as a dual-type ROS photosensitizer: mechanism, biosafety, and application in fresh-cut produce.

Background: Photodynamic inactivation (PDI) is an emerging non-thermal strategy for microbial control in food systems. However, few natural pigments are capable of generating both type I and type II reactive oxygen species (ROS), which limits their broad-spectrum antimicrobial efficacy.

Results: This study identified yam pigment (YP) as a dual-type ROS photosensitizer that can produce both singlet oxygen and free radicals under 450-nm blue light.

Exposure to diverse airborne bacterial communities following Klebsiella pneumoniae infection elicits distinct host response mechanisms in mice.

Background: Air harbors diverse microorganisms. However, the influence of airborne microbial communities on the recovery phase of pneumonia patients remains inadequately explored.

Method: We randomly generated five distinct bacterial communities with varying compositions from a pool of 16 bacteria.

Facilitating Anodic Ammonia Oxidation over Trace Cobalt-Substituted Solid Solution of Platinum to Boost Direct Ammonia Fuel Cell up to 853.75 mW cm.

Low-temperature direct ammonia fuel cell (DAFC) is a key technology for decarbonized electric generation on a large scale and with high safety. However, as the core of DAFC, the anodic ammonia oxidation reaction (AOR) still suffers from unsatisfactory activity and an ambiguous reaction mechanism. Herein, inspired by homogeneously catalyzed oxidation of ammonia, a nucleophilic attack mechanism that loosens the steric and charge constraints in heterogeneous catalysis is theoretically predicted and experimentally verified by a three-stage isotope-labeling experiment.

Comparison of Classical P81 and SVI-P Cation-Exchange Papers in Radiometric Protein Kinase Assays.

Radiometric kinase assays have been widely used due to their high sensitivity and dynamic range. The assay measures the transfer of 32Pi from [γ-32P]-ATP to specific substrates, typically synthetic peptides. The 32P-phosphorylated peptide product is captured by binding it to phosphocellulose paper, specifically P81.

PDGFR-β/Cav1-induced autophagy via mTOR/FIP200/ATG13 activation in cancer-associated fibroblasts promotes the malignant progression of breast cancer.

Background: Breast cancer incidence rates have been increasing globally. Cancer-associated fibroblasts (CAFs), key stromal components of the tumor microenvironment (TME), play crucial roles in tumor growth by dynamically interacting with cancer cells. Autophagy has been extensively studied in multiple stages of the metastatic cascade.

TRIM21-Driven Degradation of BRD4: Development of Heterobifunctional Degraders and Investigation of Recruitment and Selectivity Mechanisms.

TRIM21 is a highly efficient and versatile E3 ubiquitin ligase that plays a crucial role in targeted protein degradation through its specific binding capabilities. To investigate its role in chemically mediated degradation, we utilized two high-affinity binders identified from a DNA-encoded compound library (DEL) to design and synthesize several TrimTACs (TRIM21-based degraders) targeting BRD4. Degradation assays revealed that TrimTACs incorporating distinct TRIM21-binding moieties produced markedly different levels of BRD4 degradation.

Global, Regional, and National Burden of Traumatic Brain Injury, 1990-2021: A Systematic Analysis for the Global Burden of Disease Study 2021.

Traumatic brain injury (TBI) is a major global health concern, contributing substantially to mortality and disability. While previous studies have reported the global and regional burden of TBI, few have explored its long-term trends, cause-specific burden, sociodemographic disparities, and future projections in a comprehensive framework. To address this gap, we conducted a retrospective analysis using the Global Burden of Disease 2021 data to estimate TBI incidence, prevalence, and years lived with disability across sex, age, and Sociodemographic Index (SDI) quintiles.

Preparation and immunoactivity of sulfated glucan from liposomes.

This study optimized the preparation conditions of sulfated glucan from liposomes (SGSCL) and evaluated its effect on immune activity. SGSCL was prepared using the reverse evaporation method, and its immune activity was assessed by measuring the proliferation of chicken spleen lymphocytes, hemagglutination inhibition (HI) antibody titers, and serum cytokine concentrations in chickens vaccinated with the Newcastle disease (ND) vaccine. The optimal preparation conditions were a phospholipid-to-cholesterol mass ratio of 5.

Bader Charge Balance Mechanism Realizes Industrial-Grade Current Hydrogen Production.

The hydrogen production technology of water splitting under a high current density is the key to solve the efficient utilization of hydrogen energy. However, it is difficult for existing catalysts to exhibit bifunctional high-current activity in the same electrolyzer, considering that the bimetallic site can endow the catalytic material asymmetry and heterogeneity and then change the intrinsic electronic structure. Herein, we constructed a La-Fe dual-site coupled self-assembled membrane electrode (D-LaFe-SAME), and the introduction of the dual site reduced the Bader charge value of the La site from 0.

The effect of Artificial Intelligence Health Education Accurately Linking System on childhood asthma: study protocol for a pilot randomized controlled trial.

Background: Childhood asthma is a prevalent chronic disease that affects millions of children worldwide. Managing this disease demands not only medical treatment but also the long-term self-management efforts of both children and their parents. Conventional self-management education typically depends on face-to-face approaches, often failing to take into account the personalized requirements and ongoing support needed.

Fluid management during pediatric lung transplantation: a single-center experience.

Background: The impact of intraoperative fluid balance on postoperative outcomes in pediatric lung transplantation (LTx) has not been conclusively established. This study aimed to investigate the effect of fluid balance on postoperative outcomes in pediatric LTx, while also sharing insights from our clinical experiences in fluid management.

Methods: We reviewed the medical records of children who underwent LTx from July 2019 to August 2023.

Pulsatilla Powder Ameliorates Damp-Heat Diarrhea in Piglets Through the Regulation of Intestinal Mucosal Barrier and the Pentose Phosphate Pathway Involving G6PD and NOX.

Damp-heat diarrhea (DHD) in piglets presents as diarrhea and intestinal bleeding, significantly affecting both piglet health and the pig industry. Pulsatilla powder (PP), a herbal formulation composed of Pulsatilla, Rhizoma Coptidis, Phellodendron Bark, and Fraxini Cortex, has proven to be an effective treatment for DHD. Although the pentose phosphate pathway (PPP) has been associated with its therapeutic effects, the exact mechanism of action remains unclear.

Significant Cross-Contamination Caused by Cooking Fume Transport between Dwelling Units in Multilayer Buildings.

Cross-contamination in multiunit residential buildings is an inevitable but poorly studied issue. We conducted a 2-month monitoring campaign in a multilayer residential building, identifying 53 interunit kitchen exhaust transmission events (∼2 per day), causing enhanced exposure of particulate matters (PM), black carbon (BC), NO, and CO and volatile organic compounds (VOCs) in both the kitchen and living room. These events resulted in a 40-80% increase in PM deposition in the respiratory systems for occupants in the living room, especially fine particles depositing in the alveolar region.

GrameneOryza: a comprehensive resource for Oryza genomes, genetic variation, and functional data.

Rice is a vital staple crop, sustaining over half of the global population, and is a key model for genetic research. To support the growing need for comprehensive and accessible rice genomic data, GrameneOryza (https://oryza.gramene.

Room-temperature phosphorescent carbonized polymer dots as superior photoresponsive oxidase mimics for colorimetric-fluorescence dual-mode enzyme-free sensing of uric acid.

Photoresponsive nanozymes are widely used in analysis and other fields owing to their controllable catalysis and low cost, but most have relatively low catalytic efficiency. In this study, room-temperature phosphorescent carbonized polymer dots (PCPDs) were optimally prepared through a one-pot hydrothermal method using polyacrylic acid and ethylenediamine as precursors. Their photoresponsive oxidase-like activity was confirmed for the first time.

Multiphase Reaction of Ozone with HONO/NO on Surfaces: Effects on Indoor HONO and Ozone.

Nitrous acid (HONO) and ozone (O) are two important indoor pollutants that affect the indoor oxidation capacity. Previous field studies have observed an inverse correlation between these two pollutants indoors, but the specific mechanism remains unclear. Given the semivolatile behavior of HONO, a possible mechanism is its multiphase reaction with ozone.