Comparative Subchronic Oral Toxicity of Gardeniae Fructus and Shuizhizi Water Extracts in Sprague-Dawley Rats Over 90 Days.

To compare the safety profiles of Gardeniae Fructus (Zhizi) and its counterfeit, Shuizhizi, Sprague-Dawley rats were orally administered aqueous extracts of Zhizi and Shuizhizi at 1.8 and 5.4 g/kg/day for 28 and 90 days, followed by a 28-day recovery period.

Analysis of obstetric-related factors influencing the occurrence of puerperal depression: A retrospective study.

Postpartum depression is a mood disorder characterized by feelings of sadness, hopelessness, fatigue, and anxiety that persist beyond the typical "baby blues" period. This study examines the prevalence of puerperal depression and its associated obstetric factors. A total of 208 mothers who delivered in the obstetrics department of this hospital from January 2020 to December 2023 were followed up during the puerperium in this retrospective cohort study.

Lycium barbarum polysaccharides alleviate experimental autoimmune prostatitis by putrescine/TRAF/JAK/STAT-mediated Th17 differentiation.

Background: Due to its often refractory nature, chronic prostatitis (CP) is a prevalent disorder that notably impacts the quality of life of 20-40-year-old men worldwide. Lycium barbarum polysaccharides (LBPs) have a broad spectrum of biological activities, such as immune modulation and the ability to reduce blood glucose and lipid levels. However, whether they modulate the gut microbiota and metabolites to exert their anti-inflammatory effects remains to be explored.

Global and regional mortality rate statistics of mpox (formerly monkeypox): a comprehensive systematic review and meta-analysis.

The mortality trends associated with the mpox virus (MPXV) are not well defined. This study aims to systematically assess the mortality rate of mpox and its stratification across different geographical locations. Comprehensive review of articles from the PubMed, Embase, Scopus, and Web of Science databases up to June 2025, focusing on studies reporting mortality rates among patients diagnosed with mpox.

Coach-Supported Internet-Based Cognitive Therapy for Social Anxiety Disorder in Hong Kong: Development, Training, and Pilot Clinical Outcomes.

Internet-based Cognitive Therapy for Social Anxiety Disorder (iCT-SAD), a treatment that is normally supported by therapists, has been modified for delivery by trained coaches without mental health practitioner qualifications and has shown promising efficacy for patients in Hong Kong.

Pan-Cancer Integrative Analyses Reveal the Crosstalk Between the Intratumoral Microbiome, TP53 Mutation and Tumour Microenvironment.

Accumulating evidence suggests that the TP53 mutation, intratumoral microbiome, and tumour microenvironment (TME) are closely linked to tumourigenesis, yet the biological mechanisms underlying these connections remain unclear. To explore this, we collected multi-omics data-including genome, transcriptome, and tumour microbiome data-from a wide range of cancer types in The Cancer Genome Atlas (TCGA). Through a pan-cancer analysis, we identified significant correlations between intratumoral microbiota diversity and TP53 mutation status, particularly in hepatocellular carcinoma (HCC) and endometrial cancer (EC).

[Construction and optimization of the artificially enhanced promoter TCIN5B].

To construct stress-responsive promoters, we mined the transcriptome data of the industrial strain A223 under stress. The transcription factor CIN5 showed significantly increased expression under stress but exhibited limited resistance. Further analysis of CIN5-interacting genes revealed that the binding motif "TTACGTAATC" (named CIN5BS) of CIN5 displayed transcription-enhancing activity.

An examination of the decoupling effect and influential mechanisms of energy consumption and economic growth in Chinese urban areas.

Under the "dual-carbon" target and the shift towards a high-quality economy, achieving a decoupling of energy consumption from economic growth is crucial. This study investigates the decoupling effect and the spatial-temporal dynamics of energy use and economic development across 282 prefecture-level cities in China from 2006 to 2021, employing the Tapio decoupling model. To thoroughly assess the factors influencing energy decoupling, a geographically weighted Durbin model was constructed based on an extended STIRPAT model.

Associations between perceived stress profiles, social connection and work engagement in clinical registered nurses: a mediation analysis and generalized additive models.

Background: Investigating the links between individual perceived stress, social connection, and work involvement plays a crucial role in enhancing the psychological health and overall well-being of clinical nurses, as well as elevating the quality of nursing care in clinical settings. This study incorporates the concepts of social connection and work engagement. By considering the potential heterogeneity of variables and applying a mediation model, we identified the influence pathways through which subgroups of perceived stress affect social connection and work engagement.

Natural polysaccharides in colloidal drug delivery systems for brain glioma therapy: Mechanisms and advancements.

Brain gliomas, particularly glioblastoma (GBM), are a category of brain tumors that are challenging to treat and characterized by low survival rates. Despite improvements in existing treatment options, challenges such as drug resistance, tumor heterogeneity, and the impediment of the blood-brain barrier to effective drug delivery remain prevalent. In recent years, natural polysaccharide polymers have attracted increasing interest in the treatment of GBM owing to their biocompatibility and potential for targeted delivery.

Biomarkers via liquid biopsy for distinguishing malignant from benign pulmonary ground-glass nodule.

The differentiation between benign and malignant pulmonary ground-glass nodules (GGNs) has always been a current clinical hotspot issue. Patients with pulmonary GGNs from 8 centers were enrolled to identify biomarkers for malignancy and benignity discrimination, and an integrated biomarker panel comprising two miRNA, one long non-coding RNA (lncRNA), and one circular RNA (cirRNA) identified by multivariate logistic regression analysis were established. The classifier had area under the receiver-operating characteristic (ROC) curve (AUC) of 0.

SmGATA15 interacts with SmMYC2 to positively regulate cold stress tolerance by modulating chlorophyll accumulation in Solanum melongena.

Chlorophyll homeostasis plays a pivotal role in plant adaptation to cold stress by maintaining photosynthetic efficiency and reducing oxidative damage. However, mechanisms regulating chlorophyll biosynthesis in eggplant (Solanum melongena L.) under cold stress are imperfectly known remain unclear.

Application of KLDS 1.1105 Postbiotics for Resisting Pathogenic Bacteria Infection in the Intestine.

Postbiotics, defined as metabolites produced by probiotics, encompass both bacterial cells and their metabolic byproducts, and offer significant health benefits to the host. However, there are relatively few reports on their effects on intestinal microbiota. In this study, we investigated the components, total antioxidant capacity of postbiotics () and their impact on intestinal flora using the Simulator for Human Intestinal Microecology Simulation (SHIME).

A telomere-to-telomere genome of wild soybean with resistance to soybean cyst nematode X12.

The soybean cyst nematode (SCN) is one of the most destructive pests affecting soybean production worldwide. Wild soybean (Glycine soja) germplasm offers valuable genetic resources for developing SCN-resistant cultivars. Here, we present a telomere-to-telomere assembly of the wild soybean Glycine soja accession YSD56, which is resistant to the highly virulent SCN race X12.

Multi-channel polarization conversion THz beam splitter based on phase gradient metasurface.

In terahertz (THz) optical systems, beam splitters are essential components. However, they still have challenges in multi-channel manipulation of THz waves. Multi-channel beam splitters are devices that simultaneously split and independently control beams through multiple channels.

Efficacy and safety of novel airway balloon cryoablation system for malignant central airway obstruction: a prospective, multicentre, randomised, non-inferiority study.

Background: A novel airway balloon cryoablation (ABC) system using liquid nitrogen as cryogen was developed for bronchoscopic intervention in malignant central airway obstruction (MCAO). This study aimed to evaluate the efficacy and safety of this system.

Methods: This was a prospective, randomised, controlled, non-inferiority study that enrolled MCAO patients at 15 sites in China.

Pore Engineering for Directional CO Enrichment in Urea Electrosynthesis.

Electrochemical synthesis of urea from CO and nitrate offers a sustainable pathway to address both carbon emissions and nitrogen pollution. However, achieving high C-N coupling selectivity remains challenging due to competing hydrogen evolution reactions and insufficient CO utilization. Herein, we implement a nanopore-structure engineering strategy to precisely tailor pore length and surface chemistry in metal-free porous carbon frameworks.

Propolis as a functional food ingredient: Modulation of gut microbiota and implications for chronic disease management.

Modulating gut microbiota has become a promising strategy for managing or treating chronic diseases, including metabolic disorders, cancer, and central nervous system conditions, with significant implications for food and nutrition fields. Propolis, a natural bee product widely used as a functional food and dietary supplement, exhibited diverse bioactivities in vitro and in vivo, including clinical trials. Recent evidence suggests that propolis can reshape gut microbial communities by influencing both probiotic and pathogenic bacteria, highlighting its potential to improve gut health and overall well-being through dietary interventions.

Identification and functional characterization of Cyprinus carpio cGAS reveals its pivotal role against viral infection.

Mammalian cyclic GMP-AMP synthetase (cGAS) exerts a pivotal role in innate immune response to viral infection by detecting viral DNA and activating the cGAS-STING pathway, which triggers type I interferon (IFN-I) production. Although cGAS has been widely investigated in mammals, its function in lower vertebrates, especially in teleost fish, remains poorly understood. Herein, a cGAS homolog (termed c-cGAS) in common carp was identified and functionally characterized to explore its involvement in immune activation and viral response.

Remimazolam is more suitable for general anesthesia bronchoscopy in tuberculosis patients treated with isoniazid: a retrospective cohort analysis.

Objective: The objective of this study was to assess the suitability of remimazolam compared to propofol for general anesthesia during tracheoscopic procedures in patients with tuberculosis undergoing treatment with isoniazid.

Patients And Methods: A retrospective analysis was conducted on 1,098 patients diagnosed with pulmonary tuberculosis who underwent bronchoscopic general anesthesia at our institution between January and June 2023 (Protocol No. 2022LY0416).

Copper Sulfide Nanoparticle-Mediated Photothermolysis Induces Immunogenic Cell Death in Ovarian Cancer Cells.

Immunotherapy has only limited efficacy against ovarian cancer because of dysfunctional T cells in an immunosuppressive tumor microenvironment. Selective photothermolysis is a technique in which short pulsed laser is used to ablate tissue in targeted regions in a precise spatiotemporally controlled manner. This technique allows targeted tissue ablation without damage to surrounding tissue.

Position-dependent spin-dynamics in cobalt-substituted graphene nanoflakes.

Using quantum chemical calculations, we systematically investigate the ultrafast spin dynamics in a rhombic graphene nanoflake substitutionally doped with a Co atom (Co/GNF). We put particular emphasis on the position dependence of spin-flip processes. Our calculations reveal that when the Co dopant is placed near the zigzag boundary (N-type), spin-flip processes can be accomplished within 0.

Distinct effects of three knee-preserving surgeries on hip-knee-ankle alignment in patients with knee osteoarthritis.

Background: Unicompartmental knee arthroplasty (UKA), high tibial osteotomy (HTO), and distal femoral osteotomy (DFO) are common knee-preserving surgeries for knee osteoarthritis (KOA), yet systematic comparisons of their effects on lower limb biomechanical parameters remain limited.

Objective: To retrospectively evaluate and compare the impacts of UKA, HTO, and DFO on hip-knee-ankle alignment in KOA patients, providing evidence for personalized surgical strategy formulation. We hypothesized that these procedures would exert differential effects on specific alignment parameters.

Efficacy and safety of Qishen granule for treating heart failure with preserved ejection fraction: a study protocol for a multicenter, randomized, double-blind, placebo-controlled trial.

Background: Heart failure with preserved ejection fraction (HFpEF) is a multifaceted clinical condition that comprises nearly 50% of all heart failure cases, making it a significant clinical challenge. Qishen granule (QSG) is a promising traditional Chinese medicine (TCM) treatment that shows cardioprotective potential against chronic heart failure, as evidenced in previous studies. This trial intends to perform a prospective clinical study to observe the efficacy and safety of QSG on HFpEF.