Long-term and seasonal dynamic patterns and drivers of dissolved carbon in a shallow eutrophic lake.

Dissolved carbon is a crucial component of freshwater ecosystems and plays an important role in the Earth's carbon cycle. This paper delivers a groundbreaking exploration of dissolved carbon (DOC and DIC) variations spanning 12 years in a eutrophic lake where nutrient levels are gradually declining to reveal their spatial and temporal distribution patterns and the key drivers behind this variation. Our findings indicate that both DIC and DOC concentrations in Lake Chaohu exhibit a westward spatial gradient, with an overall upward trend in DIC levels from 2012 to 2023, contrasting with a downward trend in DOC.

Sedative and echocardiographic effects of intranasal or intramuscular administration of dexmedetomidine in healthy cats.

ObjectivesThe aim of the present study was to evaluate the sedative and echocardiographic effects of dexmedetomidine (DEX) administered via intranasal (IN) and intramuscular (IM) routes in cats.MethodsThis randomised, blinded crossover study involved eight healthy adult cats. Cats were randomly allocated to receive DEX 10 μg/kg via either the IN or IM routes.

Efficacy of Pulsatilla saponin B4 for treatment dairy cows affected with clinical mastitis.

In order to explore the therapeutic effect of natural compound Pulsatilla saponin B4 (PSB4) on clinical mastitis (CM) in dairy cows, 40 dairy cows with CM and 20 healthy dairy cows were selected as experimental samples in a large dairy farm. The CM cows were randomly divided into two groups (20 cows in each group), namely the PSB4 group treated with PSB4 neck intramuscular injection and the positive drug (PC) group treated with ceftiofur sodium neck intramuscular injection as a positive control. Another 20 healthy dairy cows were neck intramuscular injected with the same amount of normal saline to serve as a normal control (NC) group.

Mediterranean diet and associated metabolite signatures in relation to MASLD progression: A prospective cohort study.

Background: Mediterranean diet (MED) is recommended for managing metabolic dysfunction-associated steatotic liver disease (MASLD). However, associations between MED adherence, related metabolite signatures, and risks of liver-related events (LRE) and mortality in MASLD patients remain unclear.

Methods: We performed a prospective analysis using UK Biobank data, including 47,429 MASLD participants free of LRE at baseline.

N-glycosylation of the PEDV spike protein modulates viral replication and pathogenicity.

Porcine epidemic diarrhea virus (PEDV), a highly virulent enteric coronavirus, induces severe watery diarrhea and mortality in suckling piglets. The spike (S) protein, a critical mediator of viral entry, undergoes extensive N-linked glycosylation. To elucidate the functional significance of these post-translational modifications, we employed a reverse genetics system to generate 19 recombinant PEDV strains with single-site mutations at predicted N-glycosylation sites.

A PBX1-IL7R axis mediates liver fibrosis in non-alcoholic fatty liver disease.

Aims: Non-alcoholic fatty liver disease (NAFLD), also known as metabolic dysfunction associated steatotic liver disease (MASLD), represents a spectrum of chronic liver diseases that eventually lead to cirrhosis and hepatocellular carcinoma. Liver fibrosis is both a pathological feature and a contributing factor in NAFLD. In the present study we investigated whether targeting pre-B cell leukemia transcription factor 1 (PBX1) and interleukin 7 receptor (IL7R) might ameliorate liver fibrosis in the context of NAFLD.

Colorimetric reverse transcription loop-mediated isothermal amplification assay for visual, sensitive, and specific detection of enterovirus G.

Background: Enterovirus G (EV-G) is widely prevalent in pigs worldwide, and co-infections of EV-G and other diarrhea-causing pathogens have been reported in many countries, threatening the pig farming industry. There are some methods available for EV-G detection; however, the RT-LAMP method for detecting EV-G has not yet been developed. The aim of thisstudy was to establish a highly sensitive and visual RT-LAMP assay for the detection of EV-G.

Detection of Porcine Circovirus Type 3 in Free-Ranging Wild Boars and Ticks in Jiangsu Province, China.

Porcine circovirus type 3 (PCV3) has been detected in wild boars across many countries in Europe, Asia, and South America. However, data regarding the presence of porcine circoviruses in wild boars and ticks remain limited. In this study, we investigated the presence and genetic characteristics of PCV3 in wild boars and parasitizing ticks in Jiangsu, China.

Fibroblast-Myofibroblast Transition in Osteoarthritis Progression: Current Insights.

Osteoarthritis (OA) is a multifactorial joint disease traditionally characterized by cartilage degradation, while growing evidence underscores the critical role of synovial fibrosis in driving disease progression. The synovium exhibits pathological remodeling in OA, primarily due to the phenotypic transition of fibroblast-like synoviocytes (FLSs) into myofibroblasts. This fibroblast-myofibroblast transition (FMT) results in excessive deposition of extracellular matrix (ECM) and increased tissue stiffness and contractility, collectively contributing to chronic inflammation and fibrotic stiffening of the joint capsule.

Development of Blocking ELISA Kit Based on Recombinant Monoclonal Antibody for the Serological Diagnosis of Peste Des Petits Ruminants Virus.

Background: Peste des petits ruminants (PPR) was a highly contagious and economically devastating disease affecting sheep and goats. The performance and cost of serological diagnostic kit remained a major challenge for PPR eradication.

Objective: The nucleocapsid (N) protein of PPR virus and its monoclonal antibody (mAb) were engineered for development of a blocking ELISA.

Acute fibrinous and organizing pneumonia induced by cat scratch disease: a first case report.

We describe the first documented case of acute fibrinous and organizing pneumonia (AFOP) induced by cat-scratch disease (CSD). A 74-year-old female patient was admitted to the hospital with progressive dyspnea and dry cough for more than 1 month that did not respond to conventional antibiotic therapy. Physical examination revealed cat bite scars on the left index finger.

Robust Sustainable Interfacial Evaporators from Lignin for Wastewater Treatment.

Solar-driven interfacial evaporation technology has emerged as a promising solution for heavy metal-containing wastewater treatment, owing to its environmentally friendly and sustainable energy input advantages. However, the development of low-cost, high-efficiency photothermal agents with enhanced heavy-metal ion tolerance remains a significant challenge for advancing practical solar-powered wastewater treatment. Herein, we developed a series of metal-lignin composites, through a one-pot reaction involving lignin and heavy metal-contained wastewater, which demonstrated outstanding light-harvesting efficiency and photothermal conversion properties.

A novel method for high-precision tungsten isotope measurements on small-size samples.

The application of Hf - W system for accurate dating and tracing of planetary events relies on highly accurate and precise tungsten isotope measurements. However, high-precision measurement of W isotopes usually requires large samples, which greatly hampers the study of low-content and/or small-size samples. In this study, a two-column W purification procedure was developed for small-size samples with the whole procedure blank of less than 40 pg and the recovery greater than 97 %.

Erianin induces GSDMD-dependent pyroptosis and synergistically enhances doxorubicin efficacy via the PI3K/AKT signaling pathway in diffuse large B-cell lymphoma.

Background: Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma and is characterized by high aggressiveness and rapid growth. Erianin, a natural compound derived from the Chinese herb Dendrobium, has been shown to exhibit anticancer effects in certain types of cancer; however, its role and mechanism of action in DLBCL have not yet been reported. Therefore, this study aimed to investigate the potential of erianin as a therapeutic drug for DLBCL.

Biomarkers via liquid biopsy for distinguishing malignant from benign pulmonary ground-glass nodule.

The differentiation between benign and malignant pulmonary ground-glass nodules (GGNs) has always been a current clinical hotspot issue. Patients with pulmonary GGNs from 8 centers were enrolled to identify biomarkers for malignancy and benignity discrimination, and an integrated biomarker panel comprising two miRNA, one long non-coding RNA (lncRNA), and one circular RNA (cirRNA) identified by multivariate logistic regression analysis were established. The classifier had area under the receiver-operating characteristic (ROC) curve (AUC) of 0.

Case Report: Compound heterozygous variants in cause complex syndactyly with oligodactyly, renal artery variation, and facial scar.

Background: The gene, a member of the basic helix-loop-helix (bHLH) family of transcription factors, plays a critical role in limb development. Mutations in have been associated with various limb malformations, including syndactyly and split-hand/foot malformation. This study aimed to identify and characterize novel variants in a fetus with complex limb and renal abnormalities, providing further insights into the genetic basis of developmental disorders.

Thoracic Paravertebral Block with Liposomal Bupivacaine Versus Plain Bupivacaine in Patients Undergoing Thoracoscopic Lung Resection: A Randomized Controlled Study.

Background: The effectiveness of a thoracic paravertebral blockade with liposomal bupivacaine for thoracic surgery pain management is not well examined. This study compared the effects of liposomal bupivacaine and plain bupivacaine on a thoracic paravertebral blockade in adult patients undergoing video-assisted thoracoscopic surgery (VATS).

Methods: Consenting participants (114) scheduled for VATS were randomly assigned to thoracic paravertebral blockade at T4-5 and T7-8 levels with 20 mL (266 mg) liposomal bupivacaine (LB) or 20 mL (37.

A Miniaturized Centrifugal Pump ECMO System Enhances Hemocompatibility in Small Animal Models.

Extracorporeal membrane oxygenation (ECMO) is a fundamental treatment for cardiovascular and severe pulmonary diseases, with small animal models providing critical insights into organ protection during cardiopulmonary bypass and ECMO therapy. Conventional roller pumps, however, induce hemolysis and organ injury through repetitive compression-shear exposure, severely limiting their utility. While centrifugal pumps reduce shear stress and are effective in large animal ECMO, their flow range and priming volume are unsuitable for small animals.

Loss of Prominin 2 expression inhibits AKT/mTOR signaling to limit glycolysis and drive ferroptosis in breast cancer cells.

This study aimed to characterize the oncogenic functions of Prominin 2 (PROM2), the pro-cancer and ferroptosis resistance gene, in breast cancer (BC). PROM2 expression was analyzed using single-cell RNA sequencing and the TCGA database. Its expression was confirmed in BC tissues and cell lines using qRT-PCR, immunohistochemistry, and western blot assays.

Inhibition of tumor cell macropinocytosis driver DHODH reverses immunosuppression and overcomes anti-PD1 resistance.

Tumor cells' macropinocytosis sustains their growth under nutrient-limiting conditions. However, the metabolic regulation of cancer macropinocytosis in immune escape and its effect on immunotherapy remain unclear. Through the metabolism compound library and genome-wide CRISPR-Cas9 screenings, we identified dihydroorotate dehydrogenase (DHODH) as an essential driver of tumor cell macropinocytosis.

FOXP2/SOS1/AKT negative feedback loop inhibits cell proliferation in KRAS-mutant colorectal cancer.

FOXP2, a member of the Forkhead box transcription factor family, has been implicated in diverse biological processes and malignancies. However, its role in colorectal cancer (CRC), particularly in the context of KRAS mutations, remains poorly defined. Here, we analyzed FOXP2 expression in CRC datasets and clinical specimens, and conducted functional assays-including colony formation, cell viability, EdU incorporation, and cell cycle analysis-in KRAS-mutant CRC cell lines with FOXP2 overexpression or knockdown.

tsRNADisease: a manually curated database of tsRNAs associated with human disease.

tRNA-derived small RNAs (tsRNAs), as a class of regulatory small noncoding RNA, have been implicated in a wide variety of human diseases. Large amounts of tsRNA-disease associations have been identified in recent years from accumulating studies. However, repositories for cataloging the detailed information on tsRNA-disease associations are scarce.

Quercetin ameliorates epithelial-mesenchymal transition and inflammation by targeting FSTL1 and modulating the NF-κB pathway in pulmonary fibrosis.

Background: Idiopathic pulmonary fibrosis (IPF) is a progressive disorder characterized by chronic inflammation and pathological lung remodeling driven by excessive extracellular matrix deposition. While the flavonol quercetin exhibits established anti-inflammatory and antioxidant properties, its therapeutic mechanisms against IPF-particularly regarding epithelial-mesenchymal transition (EMT) and inflammation regulation via the follistatin-like 1 (FSTL1)/nuclear factor kappa B (NF-κB) axis-remain incompletely elucidated. This study therefore investigates quercetin's capacity to mitigate pulmonary fibrosis through targeted modulation of the FSTL1/NF-κB pathway.

Across ice-water-air visible light communications.

Visible light communication (VLC) technology has gained significant attention due to its abundant spectrum resources and high channel capacity for future LiFi and underwater optical wireless communication applications in the 6 G era. Aiming at ubiquitous wireless communication, the study of VLC across different media becomes an important research topic. In particular, the optical wireless communication in ice remains limited.

Generation of multipartite entanglement in a cavity-magnomechanical system.

Generating multipartite entanglement is always a popular research field in quantum information processing and quantum optics. In this paper, we propose a scheme to realize all bipartite entanglements and the tripartite entanglement among photon, magnon, and phonon modes in a cavity-magnomechanical system. The center-of-mass motion of a yttrium iron garnet sphere couples to the magnon and constitutes photon-magnon-phonon tripartite interaction.