Dual-Mode Hybrid Discharge Plasma-Activated Injectable Hydrosol for Enhanced Immunotherapeutic Cancer Therapy.

Although cold atmospheric plasma is a promising therapeutic technique for tumor immunotherapy via reactive oxygen and nitrogen species (RONS), the challenges associated with the generation and delivery of these RONS hamper clinical adoption. Herein, a dual-mode hybrid discharge plasma-activated sodium alginate hydrosols (PAH) is proposed to enhance the antitumor immune response. Gaseous highly reactive RONS are generated by dual-mode hybrid plasma produced by mixed O and NO modes, which are converted into aqueous RONS in PAH via gas-liquid reactions between plasma and hydrosols.

Low-Energy Femtosecond Laser Lens Fragmentation Techniques for Cataract.

Femtosecond laser has become an important tool in cataract surgery due to its precision and minimally invasive nature. However, the strong scattering properties of the lens limit surgical efficiency, especially when it comes to effectively cutting highly opaque and hard cataracts with femtosecond laser. This study proposes a method for achieving optical transparency in opaque lenses through tartrazine treatment.

AI-augmented prediction of high-risk PINK1 variants associated with Parkinson's disease: integrating multilayered bioinformatics, MD simulation, and deep learning.

Parkinson's disease is a prevalent neurodegenerative disease, in which genetic mutations in many genes play an important role in its pathogenesis. Among these, a mutation in the PINK1 gene, a mitochondrial-targeted serine/threonine putative kinase 1 that protects cells from stress-induced mitochondrial dysfunction, is implicated in autosomal recessive Parkinsonism. However, the exact etiology is not well understood.

PEDUNCLE LENGTH 1 regulates wheat peduncle elongation through brassinosteroid signaling pathway.

This study identifies TaPL1, a MADS-box transcription factor underlying the QFiriti-6B QTL, as a key regulator of peduncle elongation in wheat. TaPL1 enhances brassinosteroid signaling through direct suppression of TaBKI1, and its loss-of-function alleles exhibit reduced plant height and peduncle length, but increased grain weight, offering valuable targets for yield improvement in wheat breeding.

Lactococcus formosensis and its metabolite 4-acetamidobutanoic acid induced caspase-11 dependent myenteric neuronal pyroptosis in intractable functional constipation.

Intractable functional constipation (IFC), a severe form of chronic constipation characterized by slow transit and resistance to conventional treatments, posed a significant clinical challenge. Here, we identified Lactococcus formosensis (Lf), a Gram-positive bacterium prevalent in IFC patients, as a novel contributor to intestinal motility impairment. Clinically, IFC patients exhibited increased colonic mucosal colonization of Lf and significant myenteric neuronal loss and pyroptosis, particularly in excitatory choline acetyltransferase (ChAT) neurons, but not inhibitory neuronal nitric oxide synthase (nNOS) neurons.

[Determination of 79 pesticide residues in fruits and vegetables by QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry].

Pesticide residues in fruits and vegetables are becoming a serious issue. These residues can affect the quality of agricultural products and people's health. Therefore， it has become crucial to effectively monitor and control pesticide residues in the food safety field.

Physical health and cognitive ability factors in predicting retirement adjustment based on machine learning approach: results from the China Health and Retirement Longitudinal Study.

Introduction: Retirement is one of the most significant status changes in an individual's later life. Physical health and cognitive ability are key predictors of retirement adjustment. However, studies have yet to investigate the role of different physical health and cognitive ability indicators simultaneously, and their non-linear association in relation to retirement adjustment.

Intrinsic relationship between structural changes of different types of oyster proteins and flavor release after sous-vide treatment.

Proteins, as typical macromolecules in aquatic products, have been demonstrated to play a crucial role in flavor release. The structural modifications of water-soluble proteins (WSP), salt-soluble proteins (SSP), and alkali-soluble proteins (ALSP) in oysters after Sous Vide (SV) heating, along with their interactions with flavor compounds, were systematically investigated. More significant alterations in secondary and tertiary structures in SSP were observed compared to WSP and ALSP following SV heating, accompanied by the highest degree of hydrophobicity and aggregation.

Rapid screening of glyphosate in environmental water based on Ponceau 4R-Cu competitive complexation.

Glyphosate, a widely used organophosphorus herbicide in agriculture, poses potential threats to aquatic ecosystems and human health due to its long-term environmental persistence. This study presents a spectroscopic detection system based on a competitive reaction utilizing the Ponceau 4R (P4R)-Cu complex. Leveraging glyphosate's high affinity for chelating copper ions, the method enables indirect, rapid, and visual quantitative analysis of glyphosate.

Somatization symptoms and burnout: a correlational study among emergency nurses.

Background: Somatic symptom disorder is influenced by various factors, with increasing evidence highlighting its close association with burnout. This study aimed to investigate the correlation between somatization symptoms and burnout levels among emergency nurses, focusing on the impact of burnout on somatization.

Methods: A cross-sectional study was conducted involving 1,540 emergency nurses working in tertiary hospitals in China.

Effects of Dexmedetomidine on Postoperative Quality of Recovery and Electroencephalogram in Elderly Patients Undergoing Total Knee Arthroplasty: A Randomized Clinical Trial.

Purpose: Anesthesia management strategies in elderly patients undergoing total knee arthroplasty (TKA) are critical to the postoperative quality of recovery (QoR), and changes in electroencephalogram (EEG) associated with anesthesia drugs may play an important role in this process. This study aimed to determine the effects of different doses of dexmedetomidine on postoperative QoR in elderly TKA patients, and whether there is a correlation with specific EEG changes.

Methods: In this randomized controlled trial, elderly patients (aged ≥ 60 years) undergoing elective TKA were randomly allocated in a 1:1:1 ratio to 0.

Adsorption and interaction mechanisms of asphaltene subfractions on silica surfaces.

Adsorption of asphaltenes onto mineral solids contributes to fouling, scaling, and plugging issues in the oil industry. Among asphaltene subfractions, those with strong oil/water interfacial activity are expected to possess superior adsorption abilities on mineral surfaces. In this study, interfacially non-active (INAA) and active (IAA) fractions were separated from whole asphaltenes.

Synthesis of flexible SERS sensing platform of graphene/Ag-popcorns/ PMMA for ultrasensitive detection.

Surface-enhanced Raman scattering (SERS) with ultrahigh sensitivity has garnered significant attention for quantitative analysis and chemically specific detection. However, conventional SERS platforms, typically structured by depositing plasmonic micro/nanoparticles onto rigid substrates, face limitations in further advancement and applications. In this study, we present a novel method to prepare a flexible SERS film substrate composed of graphene overlayer (G) atop a self-assembled array of silver micropopcorns (Ag MPs) and a polymethyl methacrylate (PMMA) membrane.

TGFB2/IGFBP5 activated by transcription factors STAT2 and SMAD3 initiate granulosa cell degeneration and cause follicular atresia in chickens.

Follicular atresia is a crucial factor that affects the egg-laying performance of chickens, and granulosa cells (GCs) are the root cause of follicular atresia. Investigating the fate-determining genes of GCs can help improve egg production and laying period duration in chickens. Notably, transforming growth factor beta 2 (TGFB2) and insulin like growth factor binding protein 5 (IGFBP5) were previously identified as key genes responsible for GC degeneration.

Systematic assessment of landfill mining potential in Zhejiang Province, China: Resource recovery and economic potential.

Landfilling has been the predominant municipal solid waste disposal method in recent decades. Recently, landfill mining has gained attention due to increasing raw material costs and environmental concerns. However, most research has focused on individual or limited specific cases, with insufficient studies on landfill mining potential at the regional or national level.

Prospects of Nanoscience with Nanocrystals: 2025 Edition.

Nanocrystals (NCs) of various compositions have made important contributions to science and technology, with their impact recognized by the 2023 Nobel Prize in Chemistry for the discovery and synthesis of semiconductor quantum dots (QDs). Over four decades of research into NCs has led to numerous advancements in diverse fields, such as optoelectronics, catalysis, energy, medicine, and recently, quantum information and computing. The last 10 years since the predecessor perspective "Prospect of Nanoscience with Nanocrystals" was published in ACS Nano have seen NC research continuously evolve, yielding critical advances in fundamental understanding and practical applications.

Complete remission of recurrent gastric cancer in a young female patient through CLDN18.2-targeted therapy (LM302) and metastatic ovarian tumor resection: a case report of refractory disease overcoming chemotherapy and immunotherapy resistance.

Young patients with recurrent, metastatic gastric cancer (GC) resistant to chemotherapy and immunotherapy have poor outcomes and limited treatment options. CLDN18.2 has emerged as a promising target in GC.

Dual-Layer Flexible Liquid Metal Fiber for Plantar Pressure Monitoring and Synchronous Wound Electrotherapy.

Plantar wounds, particularly diabetic foot ulcers (DFUs), impose significant burdens on patients' quality of life and healthcare systems. Personalized wound management demands real-time monitoring of biomechanical parameters and effective therapeutic interventions to prevent exacerbations. Here, a dual-layer flexible liquid metal fiber (LMF) capable of simultaneous plantar pressure sensing and electrical stimulation for accelerated wound healing is presented.

Partial Reprogramming in Senescent Schwann Cells Enhances Peripheral Nerve Regeneration via Restoration of Stress Granule Homeostasis.

Partial reprogramming (pulsed expression of reprogramming transcription factors) ameliorates multiple tissue functions in aged mice; however, its impact on peripheral nerve regeneration remains largely unexplored. In this study, the temporal dynamics of Schwann cells following sciatic nerve injury in young and aged rats are systematically examined using single-cell transcriptomics to identify a Runx2 cell population highly enriched with stress granules as transitional homeostatic cells during Schwann cell differentiation. It is found that pathological accumulation of this cluster during axonal regeneration constitutes a critical contributing factor to impaired neural repair in aging.

Diweng Zhixie Power Ameliorates Dampness-Heat Diarrhea by Restoring Intestinal Microbiota Dysbiosis and Short-Chain Fatty Acids.

Current therapeutics for diarrhea have limitations. Classical Formula Diweng Zhixie power (DW) is derived from Shang Han Lun and has a long history of treating gastrointestinal diseases such as diarrhea. Nevertheless, the exact mechanism of it needs to be further clarified.

Bibliometric-driven research on chemoresistance in breast cancer: knowledge mapping, hotspot evolution, and emerging insights (1994-2024).

Background: Chemoresistance remains a pivotal challenge in the clinical management of breast cancer. A systematic dissection of global research trends, evolutionary hotspots, and emerging frontiers in this field is imperative for advancing therapeutic strategies.

Methods: Relevant literature on breast cancer chemoresistance from 1994 to 2024 was retrieved from the Science Citation Index-Expanded (SCI-E) database of the Web of Science Core Collection (WoSCC).

The quorum sensing regulator Kj-LuxS modulates biofilm formation for nicosulfuron degradation and rhizosphere colonization in Klebsiella.

Soil nicosulfuron residues disrupt crop rotation with herbicide-sensitive plants. In this study, we demonstrate for the first time that the Kj-LuxS/AI-2 quorum sensing system in Klebsiella jilinsis 2N3 critically regulates biofilm-mediated nicosulfuron detoxification through multiple mechanisms: (1) Kj-LuxS deletion reduced biofilm biomass by 60.62 % and decreased herbicide biosorption by 72.

Alternate wetting and moderate soil drying (AWMD) enhances the physicochemical properties of rice starch by promoting grain-filling in inferior grains of rice.

Water scarcity is the major challenge to sustainable rice production. This study examines how alternate wetting and moderate soil drying (AWMD), a widely adopted water-saving practice, influences grain-filling dynamics and starch physicochemical properties in both superior grains (located on apical primary branches, flower earlier) and inferior ones (located on proximal secondary branches, flower later). Results showed that AWMD enhanced grain-filling in inferior grains: it increased the mean (G) and peak (G) filling rates by 26.

Dissecting the genetic architecture of yield-related traits by QTL mapping in maize.

Introduction: Maize is a cornerstone of global agriculture, essential for ensuring food security, driving economic development, and meeting growing food demands. Yet, how to achieve optimal yield remains a multifaceted challenge influenced by biotic, environmental, and genetic factors whose comprehensive understanding is still evolving.

Methods: QTL mapping of eight essential yield traits was conducted across four environments - Sanya (SY) in 2021, and Yangling (YaL), Yulin (YuL), and Weinan (WN) in 2022 - using two types of populations: a KA105/KB024 recombinant inbred line (RIL) population and two immortalized backcross populations (IB1 and IB2) derived from the RILs by crossing with their respective parents.

Augmentation of PRDX1-DOK3 interaction alleviates rheumatoid arthritis progression by suppressing plasma cell differentiation.

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent inflammation and joint damage, accompanied by the accumulation of plasma cells, which contributes to its pathogenesis. Understanding the genetic alterations occurring during plasma cell differentiation in RA can deepen our comprehension of its pathogenesis and guide the development of targeted therapeutic interventions. Here, our study elucidates the intricate molecular mechanisms underlying plasma cell differentiation by demonstrating that PRDX1 interacts with DOK3 and modulates its degradation by the autophagy-lysosome pathway.