Low-Energy Femtosecond Laser Lens Fragmentation Techniques for Cataract.

Femtosecond laser has become an important tool in cataract surgery due to its precision and minimally invasive nature. However, the strong scattering properties of the lens limit surgical efficiency, especially when it comes to effectively cutting highly opaque and hard cataracts with femtosecond laser. This study proposes a method for achieving optical transparency in opaque lenses through tartrazine treatment.

Non-invasive measurement of in vivo corneal steady-state biomechanical properties via controllable negative pressure inflation.

Objective: To measure the steady-state corneal biomechanical properties related to postoperative corneal ectasia, keratoconus, glaucoma and other ophthalmic diseases, we propose a novel in vivo measurement method.

Methods: By precisely manipulating ambient negative pressure via a suction device to achieve controlled in vivo corneal inflation, we analyzed the coupling relationship between corneal deformation response and negative pressure loading. The displacement corresponding to the corneal initial configuration under 0 mmHg was extrapolated.

H3F3B p.K27I-mutant diffuse midline glioma is a distinct subtype of H3K27-altered diffuse midline glioma.

H3K27-altered diffuse midline glioma (DMG) is a fatal disease, including four subtypes H3.3-mutant, H3.1/H3.

Natural history of Chiari I malformation-syringomyelia: longitudinal cohort study.

Background: The natural history of clinically stable patients with Chiari I malformation (CM-I)-syringomyelia is uncertain. To understand their outcomes, we examined conservatively managed CM-I-syringomyelia patients' long-term clinical and radiological courses.

Methods: We enrolled 156 mild CM-I-syringomyelia cases (Japanese Orthopaedic Association (JOA) score ≥13) managed non-surgically between 1994 and 2014 and followed them periodically until December 2024 for significant progressive myelopathy that we termed 'obvious deterioration'.

A rare B cell clonotype imprinted by ancestral SARS-CoV-2 develops cross-sarbecovirus neutralization in immune recalls.

The ultimate potential of B cells imprinted by ancestral SARS-CoV-2 in developing neutralizing breadth and potency remains to be explored. Here, we longitudinally tracked B cells that recognize the wild-type spike in two individuals who were repeatedly infected by Omicron variants after receiving prototype mRNA vaccines. Functional and genetic analysis of 632 monoclonal antibodies (mAbs) from those B cells reveals that mAbs cloned after a second infection have dramatically enhanced neutralizing breadth and potency due to immune recalls.

Sex differences in lithium concentrations among bipolar patients with different episodes based on real-world therapeutic monitoring data.

Background: Sex differences in lithium-associated efficacy and adverse events have been well studied in bipolar disorder. Exploring variations in lithium concentrations between sexes may offer potential explanations.

Methods: This multicenter, real-world, observational study, which was based on therapeutic monitoring data, was conducted at six Chinese hospitals.

Discovery of Synergistic Broadly Neutralizing Antibodies Targeting Non-Dominant Epitopes on SARS-CoV-2 RBD and NTD.

: Identification and characterization of broadly neutralizing monoclonal antibodies from individuals exposed to SARS-CoV-2, either by infection or vaccination, can inform the development of next-generation vaccines and antibody therapeutics with pan-SARS-CoV-2 protection. : Through single B cell sorting and RT-PCR, monoclonal antibodies (mAbs) were isolated from a donor who experienced a BA.5 or BF.

Body Pain and Depressive Symptoms: Patterns and Associations in Middle-Aged and Older Chinese Adults.

Depressive symptoms (DS) and body pain are prevalent conditions that significantly impact the quality of life of older adults, often co-occurring with chronic diseases. This study aimed to explore the patterns of body pain characteristics and their association with DS among middle-aged and older Chinese adults. This cross-sectional study analysed data from 16,039 participants aged ≥45 years in the 2020 wave of the China Health and Retirement Longitudinal Study (CHARLS).

Analysis of maintenance pharmacotherapy in Chinese bipolar disorder patients: Guideline concordance and adjunctive medication use.

Objective: We collected maintenance treatment medication information from Chinese multicenter bipolar disorder patients, exploring the characteristics of medication use and related factors. We analyzed medication guideline adherence and compared trends in medication use with a study from 2014.

Methods: 323 patients receiving maintenance therapy for bipolar disorder across 20 hospitals have been recruited.

Large Language Models' Responses to Spinal Cord Injury: A Comparative Study of Performance.

With the increasing application of large language models (LLMs) in the medical field, their potential in patient education and clinical decision support is becoming increasingly prominent. Given the complex pathogenesis, diverse treatment options, and lengthy rehabilitation periods of spinal cord injury (SCI), patients are increasingly turning to advanced online resources to obtain relevant medical information. This study analyzed responses from four LLMs-ChatGPT-4o, Claude-3.

Altered resting-state brain activity in patients with major depression disorder and bipolar disorder: A regional homogeneity analysis.

Background: Major Depressive Disorder (MDD) and Bipolar Disorder (BD) exhibit overlapping depressive symptoms, complicating their differentiation in clinical practice. Traditional neuroimaging studies have focused on specific regions of interest, but few have employed whole-brain analyses like regional homogeneity (ReHo). This study aims to differentiate MDD from BD by identifying key brain regions with abnormal ReHo and using advanced machine learning techniques to improve diagnostic accuracy.

A Novel Surgical Technique for Post-traumatic Syringomyelia Progressing to the Medulla Oblongata: Evidence of Upward Drainage of Central Canal Fluid Within the Spinal Cord.

Background And Objectives: The exact pathways of fluid outflow within the central canal (CC) of the spinal cord are not completely understood. The best management approach for patients with post-traumatic syringomyelia that progresses to the cranial end of the CC, also known as post-traumatic syringobulbia (PT-syringobulbia), is still a topic of debate. This study aims to introduce a new surgical indication for the foramen magnum and foramen of Magendie dredging (FMMD) procedure in patients with PT-syringobulbia and prospectively assess its surgical outcomes.

Effectiveness of Cerebellar Tonsillectomy Treatment for Revision Chiari Malformation Surgery: A Series of 63 Patients.

Background: Revision surgery for patients with persistent, recurrent, or progressive syringomyelia following Foramen Magnum Decompression for Chiari malformation with syringomyelia (CM-SM) is not uncommon and presents both strategic and technical challenges.

Methods: We conducted a retrospective study including all patients who underwent revision Cerebellar Tonsillectomy (CTL) for CM-SM between 2003 and 2023. Additionally, we performed univariate and multivariate analyses to identify possible factors contributing to failed CTL outcomes.

Differentiation of mesenchymal stem cells towards lens epithelial stem cells based on three-dimensional bio-printed matrix.

The high risks of traumatic cataract treatments promoted the development of the concept of autologous lens regeneration. Biochemical cues can influence the cellular behavior of stem cells, and in this case, biophysical cues may be the important factors in producing rapid activation of cellular behavior. Here we bio-printed mesenchymal stem cells (MSCs) using a commonly used bioink sodium alginate-gelatin blends, and investigated the induction effect of MSC differentiation towards lens epithelial stem cells (LESCs) under a combination of biochemical cues and biophysical cues.

Ancestral SARS-CoV-2 immune imprinting persists on RBD but not NTD after sequential Omicron infections.

Whether Omicron exposures could overcome ancestral SARS-CoV-2 immune imprinting remains controversial. Here we analyzed B cell responses evoked by sequential Omicron infections in vaccinated and unvaccinated individuals. Plasma neutralizing antibody titers against ancestral SARS-CoV-2 and variants indicate that immune imprinting is not consistently induced by inactivated or recombinant protein vaccines.

Analysis of Failed Posterior Fossa Decompression and an Effective Revision Surgery in Patients with Basilar Invagination and Atlantoaxial Dislocation.

Objective: The objective of this study was to analyze failed posterior fossa decompression (PFD) in patients with basilar invagination and atlantoaxial dislocation (BI-AAD). Revision surgery in these patients is challenging and has been rarely reported. In addition, the anatomical variations of the vertebral artery increase the risk of revision surgery.

Topology Optimization Driven Bone-Remodeling Simulation for Lumbar Interbody Fusion.

This study proposes a numerical approach for simulating bone remodeling in lumbar interbody fusion (LIF). It employs a topology optimization method to drive the remodeling process and uses a pixel function to describe the structural topology and bone density distribution. Unlike traditional approaches based on strain energy density or compliance, this study adopts von Mises stress to guide the remodeling of LIF.

Comparison of the Efficacy Between Standard Measurement-Base Care (MBC) and Enhanced MBC for Major Depressive Disorder: A Pilot Study.

Purpose: To validate the efficacy of enhanced measurement-based care against standard measurement-based care in patients with major depressive disorder.

Patients And Methods: In this pilot study of an ongoing multicenter cluster randomized controlled trails, 160 patients diagnosed with major depressive disorder were enrolled from 2 mental health centers, with a plan to include 12 centers in total. One hundred patients engaged in a six-month evaluation using a technology-enhanced measurement-based care tool, including assessments of clinical symptoms, side effects, and functionality at baseline, two months, four months and six months.

Gut microbiome and metabolism alterations in schizophrenia with metabolic syndrome severity.

Background: Schizophrenia (SCZ) patients undergoing antipsychotic treatment demonstrated a high prevalence and harmful effects of metabolic syndrome (MetS), which acted as the major cause of cardiovascular disease. The major clinical challenge is the lack of biomarkers to identify MetS episodes and prevent further damage, while the mechanisms underlying these drug-induced MetS remain unknown.

Methods: This study divided 173 participants with SCZ into 3 groups (None, High risk, and MetS, consisting of 22, 88, and 63 participants, respectively).

The relationship between anxiety symptoms and disturbances in biological rhythms in patients with depression.

Background: Biological rhythms denote the cyclical patterns of life activities anchored to a 24-hour cycle. Research shows that depression exhibits disturbances in biological rhythms. Yet, the relationship between these biological rhythms and concomitant anxiety symptoms is insufficiently investigated in structured clinical assessments.

Harmonic resonance and entrainment of propagating chemical waves by external mechanical stimulation in BZ self-oscillating hydrogels.

Smart polymer materials that are nonliving yet exhibit complex "life-like" or biomimetic behaviors have been the focus of intensive research over the past decades, in the quest to broaden our understanding of how living systems function under nonequilibrium conditions. Identification of how chemical and mechanical coupling can generate resonance and entrainment with other cells or external environment is an important research question. We prepared Belousov-Zhabotinsky (BZ) self-oscillating hydrogels which convert chemical energy to mechanical oscillation.

Olanzapine-induced weight gain and lipid dysfunction in mice between different gender.

As one of the most common antipsychotics, olanzapine may cause metabolic-related adverse effects, but it is still unknown how olanzapine alters lipid metabolism. In this study, we found that olanzapine-treated mice showed varying degrees of dyslipidemia, which was particularly pronounced in female mice. Based on ultra-performance liquid chromatography-quadrupole time-of-flight-MS (UPLC-Q-TOF-MS) technology and lipid metabolomics, we mapped the changes in lipid metabolism in olanzapine-treated mice and then compared the changes in lipid metabolism between male and female mice.