PLEKHF1 Induces Mitochondrial Dysfunction to Inhibit Osteosarcoma Growth and Metastasis.

Osteosarcoma (OS) is an uncommon malignancy with stagnant survival rates over the past four decades and early-stage metastasis, predominantly affecting children and adolescents. This study identified significant metabolic differences between metastatic and non-metastatic OS samples through bioinformatics analysis, highlighting key processes such as cell proliferation, mitochondrial assembly, and changes in mitochondrial membrane permeability. Among differentially expressed genes, Pleckstrin Homology And FYVE Domain Containing 1 (PLEKHF1) was the most significantly downregulated in metastatic OS samples.

Risk factors and prevalence of latent tuberculosis infection in rheumatic patients: a meta-analysis.

Objective: This study aims to evaluate the risk factors and prevalence of latent tuberculosis infection (LTBI) in patients with rheumatic diseases.

Methods: Databases including PubMed, EMBASE, Web of Science, Cochrane Library, CNKI, Vip, and Wanfang were searched. Data extraction was performed independently by two authors.

Determination of the Available Energy of Corn DDGS Fed to Pregnant Sows.

Based on an established appropriate substitution level for corn distillers dried grains with solubles (DDGSs) replacing energy-supplying components in the basal diet for pregnant sows, the substitution method was employed to determine the available energy values of corn DDGSs. In Exp. 1, forty pregnant sows (gestation day = 50 ± 5 d; body weight = 220 ± 24.

Detecting ALDH2 activity in live cells conditional metabolic labeling.

Detecting enzyme activity that catalyzes subtle functional group transformations in live cells remains a major challenge. We introduce a conditional metabolic labeling strategy for enzymatic activity detection (cMLEAD), which harnesses cellular metabolic pathways to deliver indirect yet reliable activity readouts. Unlike traditional metabolic labeling approaches relying on nonspecific incorporation of tagged biomolecules, cMLEAD employs a tagged precursor whose metabolic incorporation is strictly dependent on specific enzymatic activity, effectively transforming a metabolic labeling event into an enzyme-activity measurement.

PolyA-Bridged Aptamer for Label-Free Electrochemical Aptasensors: Comparable Performance to Thiol-Terminated Aptamer but with Sharply Reduced Cost.

Thiol-terminated aptamers (SH-Apt) enable Au-S self-assembly on Au electrodes but face great challenges due to expensive thiol modification and laborious disulfide cleavage steps. Poly adenine (PolyA)-mediated aptamers offer a simpler and cost-effective alternative, yet lack systematic comparative studies against Au-S immobilization for critical metrics in electrochemical aptasensors. In this work, we present a systematic comparative evaluation of PolyA-bridged aptamer (PolyA-Apt) and SH-Apt immobilization strategies on Au electrodes for electrochemical aptasensor, using metronidazole (MTZ) as a model target and electrochemical impedance spectroscopy (EIS) for performance assessment.

Novel biomarkers to assess mold exposure among children with asthma.

Background: Household mold is a major problem in communities which face natural disasters such as hurricanes or flooding, and in homes with other sources of significant water intrusion; a biomarker for exposure to indoor mold could support public health investigations.

Methods: We analyzed serum from 132 children with asthma living in government-subsidized housing for six microbial volatile organic compounds (2-ethyl-1-hexanol, 2-heptanone, 2-hexanone, 3-methylfuran, 3-octanone, and geosmin) using GC-MS. Fewer than 10% of the samples for three compounds (2-ethyl-1-hexanol, 2-heptanone, and 2-hexanone) were quantified below the limit of detection.

Eco-environmental effects and its spatial heterogeneity of "production-living-ecological" land use transformation with the context of urban shrinkage: an empirical analysis in the karst regions of Southwest China.

Urban growth and shrinkage are two sides of the same coin in the process of urbanization, and exploring their differentiated land use transformations and eco-environment effects is of great significance for coordinating regional land resource development and improving eco-environment quality. Taking the Southwest Karst region of China as an example, this study identifies growing and shrinking cities from 2010 to 2020 based on county-level population changes. We then conduct a comparative analysis of the spatiotemporal differentiation characteristics of land use transformation and eco-environment quality in growing and shrinking cities from the perspective of production-living-ecological space.

Impact of black carbon exposure on length of hospital stay in asthma patients: a retrospective cohort study in yulin, China.

Objective: BACKGROUND: While PM₂.₅ exposure is linked to asthma exacerbations, components-specific effects on healthcare burden remain understudied. Hospital length of stay (LOS), a critical indicator of disease severity and healthcare costs, has received limited attention in black carbon (BC) research.

Comparison of the analgesic efficacy of ultrasound-guided retrolaminar block with serratus anterior plane block in patients undergoing radical mastectomy.

Objectives: The newly introduced retrolaminar block (RLB) offers anesthesiologists an alternative regional anesthetic technique for radical mastectomy. However, few clinical studies have compared the efficacy of RLB with that of serratus anterior plane block (SAPB). This study aimed to investigate the postoperative analgesia efficacy between ultrasound-guided RLB and SAPB in patients undergoing radical mastectomy.

The westerlies-monsoon interaction shaped asymmetric lake expansions over the Tibetan Plateau in warming periods.

Modelling studies and recent observations suggest that the westerlies-monsoon interactions (WMI) have led to a spatial imbalance in lake expansions across the Tibetan Plateau (TP) during the past decades under anthropogenic warming. However, whether such imbalance reflects a short-term phenomenon or a long-term climatic trend remains unclear. Here, we present a 240,000-year lake level reconstruction from the inner TP to examine the extent to which WMI also operated for previous interglacials, natural warm regimes with large-scale climatic boundary changes.

All-optical control of photonic states in a photonic molecule.

We explore the all-optical control of photonic states in a photonic molecule using a tailored control pulse. By systematically analyzing the requirements for effective state manipulation, we demonstrate that an optimized control pulse can switch off Rabi oscillations between coupled cavities, freezing the system in either symmetric or anti-symmetric supermodes. The arrival time, amplitude, and phase of the control pulse dictate the selective freezing and precise amplitude modulation of photonic states.

A novel Minimally-Invasive technique for Non-Traumatic postoperative adhesive Syringomyelia.

Purpose: Current surgical options for treating non-traumatic adhesive syringomyelia, particularly in patients with a history of previous spinal canal surgery, lack clear standardization and frequently lead to common complications such as recurrent adhesions, tube disconnections, and neurological injuries related to myelotomy, resulting in the need for multiple revision surgeries. This study aims to introduce a novel, minimally invasive technique of modified subarachnoid-subarachnoid (S - S) bypass procedure for syringomyelia associated with postoperative arachnoid adhesions and to prospectively evaluate its surgical outcomes.

Methods: This prospective study included 11 consecutive patients with syringomyelia related to postoperative arachnoid adhesions who underwent modified S-S bypass surgery, with a mean follow-up period of 18.

Unveiling the Temporal Dynamics and Molecular Regulation Profiles of Neutrophil Extracellular Traps Following Spinal Cord Injury.

Background: Spinal cord injury (SCI) initiates secondary inflammatory processes that exacerbate tissue damage, with neutrophil extracellular traps (NETs) playing a significant role in amplifying these cascades. This study aimed to explore the temporal dynamics and key regulatory genes of NET formation in SCI to identify therapeutic targets.

Methods: We integrated two transcriptomic datasets from the GEO database to identify differentially expressed NETs-related genes (NRGs) in SCI.

Effective RNA Delivery with Aggregation-Induced Lipid Backfolding.

Lipid nanoparticles (LNPs) currently serve as a leading platform for RNA delivery. In this field, endosomal escape of LNPs is a key challenge for efficient RNA therapies. Most current strategies focus on designing ionizable lipids to enhance interactions with endosomal membranes, promoting membrane fusion and RNA release.

OsbZIP83-OsCOMT15 module confers melatonin-ameliorated cold tolerance in rice.

Cold stress is one of the most common abiotic stresses, and melatonin (Mel) is involved in the regulation of plant cold tolerance. However, the detailed mechanism underlying Mel-mediated cold tolerance remains largely unknown. Here, we reveal that caffeic acid O-methyltransferase 15 (OsCOMT15) is cold-responsive (COR) and acts as a key modulator of Mel biosynthesis to enhance rice cold tolerance at the seedling stage.

From lactate to lactylation: potential targets for the treatment of neurodegenerative diseases.

Traditionally, lactate is regarded as a byproduct of anaerobic metabolism. With the deepening of related research, the roles of lactate in cellular energy metabolism, signal transduction, and microenvironment regulation have attracted increasing attention. Against this research background, the discovery of a novel post-translational modification - lactylation modification - has further expanded its biological functions.

Gut-derived bacterial vesicles carrying lipopolysaccharide promote microglia-mediated synaptic pruning.

Introduction: Growing evidence links gut microbiota (GM) to Alzheimer's disease (AD). Elevated lipopolysaccharide (LPS) levels, a Gram-negative bacteria component, are found in AD brains, but how LPS breaches the blood-brain barrier (BBB) remains unclear. Hypotheses suggest that bacteria-derived extracellular vesicles (bEVs) may transport LPS across the BBB.

Efficacy of bevacizumab maintenance therapy in advanced ovarian cancer.

This study aimed to evaluate the efficacy of bevacizumab maintenance therapy in advanced ovarian cancer and its impact on the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3)/vascular endothelial growth factor A (VEGFA) pathway. A total of 126 patients were enrolled, divided into two groups: control (n=62) and study (n=64). The control group received neoadjuvant chemotherapy, tumor debulking surgery, and postoperative adjuvant chemotherapy.

Molecular mechanism of bacterial outer membrane lysis through cold plasma reactive species evolution.

Gram-negative bacteria, especially those with multidrug resistance, prevent the entry of antibacterial substances due to the presence of the outer membrane (OM). In response to such permeability issues, the development of novel antimicrobial technologies has focused on disrupting the bacterial OM of Gram-negative bacteria. Cold atmospheric plasma (CAP), as an emerging sterilization method, has shown promise in overcoming bacterial infection due to the reactive species generation.

Lotus fiber-derived scaffolds for enhanced cultured meat production: Quality and sustainability.

The expanding global population intensifies demand for sustainable protein sources. Cell-cultured meat (CM) offers a promising alternative to conventional meat production but faces challenges in scalability and food-grade scaffold design. Current scaffolds often fail to replicate muscle tissue's structural and mechanical properties or support large-scale CM production.

Natural history of Chiari I malformation-syringomyelia: longitudinal cohort study.

Background: The natural history of clinically stable patients with Chiari I malformation (CM-I)-syringomyelia is uncertain. To understand their outcomes, we examined conservatively managed CM-I-syringomyelia patients' long-term clinical and radiological courses.

Methods: We enrolled 156 mild CM-I-syringomyelia cases (Japanese Orthopaedic Association (JOA) score ≥13) managed non-surgically between 1994 and 2014 and followed them periodically until December 2024 for significant progressive myelopathy that we termed 'obvious deterioration'.

Grass carp reovirus VP6 inhibits IFN production by degrading TBK1 through the autophagy pathway.

The interferon (IFN) response serves as a powerful defense mechanism against viral infections in fish. However, grass carp reovirus (GCRV) is capable of evading the IFN system, although the specific mechanisms remain unclear. In this study, we report that GCRV VP6 employs an immune evasion strategy by degrading TANK-binding kinase 1 (TBK1) through the autophagy pathway, thereby inhibiting IFN activation.

Study on the photo-aging process and mechanism of polystyrene microplastics under different salinities mediated by humic acid.

Microplastics are ubiquitous in the environment, and their aging is frequently influenced by environmental factors. The dissolved organic matter (DOM) and salinity influence the aging process of microplastics. Polystyrene microplastics (PS) were employed to explore the combined influence of humic acid (HA) and inorganic salt (NaCl) on the photo-aging process of PS in this study.

Ion-Conductive Polyphosphasiloxane Networks: Constructing Robust Solid Electrolyte Interphase for SiO Anode.

Silicon (Si) is famous for its high theoretical specific capacity, natural abundance, and low reduction potential. However, enormous volume change, fast capacity decay, and poor ionic conductivity hamper the practical utilization of Si-based anodes. Until now, strategies to improve cycling performance by tailoring solid electrolyte interphase (SEI) have remained less effective, especially in high-Si content anodes.

Comparison of the Safety and Efficacy of Ciprofol Versus Propofol for Induction and Maintenance of General Anesthesia in Patients Under-going Thoracoscopic Surgery: A Prospective Randomized Controlled Trial.

Objective: Ciprofol is a novel sedative-anesthetic that functions similarly to propofol. This study aimed to evaluate the efficacy and safety of ciprofol for the induction and maintenance of general anesthesia in patients undergoing thoracoscopic surgery.

Methods: A total of 120 patients undergoing thoracoscopic surgery for pulmonary nodules under general anesthesia were randomly assigned to the ciprofol group or the propofol group.