Surface soft patch-mediated supramolecular co-assembly of lysozyme/konjac glucomannan with optimized antimicrobial performance.

Assembly is a natural process where molecules spontaneously form ordered structures with specific functions. In supramolecular co-assembly, interactions between surface soft patches drive structural changes, though this key aspect is often overlooked. In this study, we propose a strategy for supramolecular co-assembly mediated by surface soft patches and apply this approach to optimize the broad-spectrum antibacterial activity of lysozyme (LYS)-konjac glucomannan (KGM) supramolecular co-assembly.

The food-derived metabolite trimethylamine and trimethylamine-N-oxide promote colorectal cancer progression via SREBF1.

Colorectal cancer (CRC) is characterised by microbial dysbiosis. Trimethylamine (TMA) and trimethylamine-N-oxide (TMAO) are gut microbiota-derived metabolites produced from dietary precursors that are implicated in CRC progression. However, the underlying mechanisms by which TMA or TMAO affect CRC progression remain largely unexplored.

Advances of bispecific antibodies using/application in dermatology: a review.

Bispecific antibodies represent an important innovation in the field of biomedicine in recent years. Compared to monoclonal antibodies, their specific structure enables a single antibody molecule to bind to two different antigens simultaneously. This characteristic endows bispecific antibodies with more functions, regulating multiple signal pathways simultaneously, enhancing the therapeutic effect, and by infusion of targeted tumor antigens and drug carriers in advance, the contact time between the drug and normal tissues is reduced, and the toxic side effects are greatly reduced.

Assessment of the Anti-Inflammatory Efficacy and Symptom Resolution Time of Kangfuxin Liquid Combined with Metronidazole in Recurrent Oral Ulcers.

Objective: To evaluate the clinical efficacy and inflammatory modulation potential of Kangfuxin Liquid (KFXY; a traditional Chinese medicine recovery solution) in combination with metronidazole (MTZ) for the treatment of recurrent oral ulcers (ROU).

Methods: A retrospective cohort study was conducted using clinical data from 117 ROU patients admitted between January 2021 and January 2023. Patients were categorized into two groups: a control group (n=58) treated with MTZ oral adhesive patches alone, and an observation group (n=59) receiving KFXY plus MTZ.

The development and validation of postpartum hemorrhage prediction models for pregnancies with placenta previa totalis based on coagulation function indexes: a retrospective cohort study.

Background: The objective of this study is to establish and validate models that can accurately predict postpartum hemorrhage (PPH) in women with placenta previa totalis prior to undertaking cesarean delivery.

Methods: A retrospective cohort study was conducted on 306 pregnancies with placenta previa totalis delivered between January 2011 and June 2022. The pregnancies were classified into two groups, PPH group and non-PPH group, based on bleeding volume and red blood cell transfusion.

Gene augmentation therapy restores vision and preserves photoreceptors in a mouse model of CNGA1-retinitis pigmentosa.

Background: Retinitis pigmentosa (RP) is a leading cause of blindness affecting 2 million people worldwide. Mutations in cyclic nucleotide-gated channel alpha 1 (CNGA1) account for 2-8% of autosomal recessive RP with no available treatment. Here we further evaluate our previously developed Cnga1 mouse model.

Broad-spectrum antitumor analysis of the telomerase activity inhibitor TPCH derived from the human constitutively expressed protein LPTS/PinX1.

Background: The human liver-related putative tumor suppressor LPTS/PinX1 is a gene encoding a telomerase inhibitory protein. Overexpression of LPTS/PinX1 protein can inhibit the growth of multiple telomerase-positive cancer cell lines. LPTS/PinX1 has therapeutic potential for cancer.

Collectin-11 promotes fibroblast proliferation and modulates their activation status and extracellular matrix synthesis.

Introduction: Collectin-11 (CL-11), a recently described soluble C-type lectin, has been shown to stimulate cell proliferation in fibroblasts and melanoma cells. However, its broader influence on fibroblast functions and the specific receptors mediating CL-11's effects remain to be elucidated.

Methods: The EDU proliferation assay and WB detection of PCNA protein levels were used to evaluate the fibroblast proliferative effect after CL-11 stimulated.

Knowledge, Attitude, and Practice of Nurses in Preventing Complications in Patients with Nephrotic Syndrome: A Cross-Sectional Study.

Background And Aim: Nephrotic syndrome (NS) is frequently accompanied by serious complications such as edema-related infections, thromboembolism, and acute kidney injury, which can significantly impact prognosis. Nurses are essential in early identification, patient education, and implementation of preventive measures. This study explores the knowledge, attitude, and practice (KAP) levels of nurses concerning complication prevention in nephrotic syndrome and identifies relevant influencing factors.

Ion transport-triggered rapid flexible hydrovoltaic sensing.

The hydrovoltaic effect, based on interactions at the solid-liquid interface, offers a promising route for ion sensing. However, it is hampered by long response times, typically several minutes, due to slow ion diffusion equilibrium in nanochannels. Here, we demonstrate a rapid, flexible hydrovoltaic ion sensing strategy enabled by fast ion transport.

The impact of land use change on ecosystem services in the Huaihai economic zone for evaluation and Spatiotemporal characterization.

A comprehensive understanding of the impact of land use change on ecosystem services is essential for regional sustainable development. This study aims to enhance the accuracy of ecosystem service value (ESV) assessment by accounting for regional differences and to propose a novel approach for quantifying the relationship between land use change and ecosystem services. Based on land use data from the Huaihai Economic Zone (HEZ) during 1995-2020, spatially adjusted equivalence factors were employed to estimate ESV, while the coefficient of improved cross sensitivity (CICS) was developed to measure the linkage between land use and ESV.

Retinal Pigment Epithelium Transplantation in Retinal Disease: Clinical Trial Development, Challenges, and Future Directions.

Replacement of the retinal pigment epithelium (RPE) is emerging as a promising approach to treat degenerative retinal diseases, including age-related macular degeneration and Stargardt disease, in which RPE function cannot otherwise be restored. Despite the limitations of existing treatments, advances in cell sourcing and surgical methods have enabled initial human trials of RPE transplantation, with early results indicating potential efficacy. This review comprehensively examines the evolution of RPE transplantation in recent decades, highlighting the advantages and limitations of different cell sources and delivery methods.

MAVM-UNet: multiscale aggregated vision MambaU-Net for field rice pest detection.

Pests in rice fields not only affect the yield and quality of rice but also cause serious ecological and environmental problems due to the heavy reliance on pesticides. Since various pests have irregular and changeable shapes, small sizes, and complex backgrounds, field rice pest detection is an essential prerequisite and challenge for the precise control of pests in the field. A multiscale aggregated vision MambaU-Net (MAVM-UNet) model for rice pest detection is constructed.

The accuracy and real resolution of karyotyping technique in detecting chromosomal aberrations identified by molecular genetic methods.

Although karyotyping technology has been implemented in genetic diagnosis for a long time, the comprehensive understanding of this technology is still limited. The aim of this study was to investigate the accuracy and resolution of karyotyping method in detecting chromosomal abnormalities by molecular genetic method. In this study, we conducted a retrospective analysis of embryonic molecular karyotypes and peripheral blood cytogenetic karyotypes from patients with balanced chromosomal rearrangements undergoing preimplantation genetic testing at our reproductive center.

Breaking antibiotic resistance: Sunlight-powered calcium peroxide for dual bactericidal and genetic elimination.

Antibiotic-resistant bacteria (ARB) and associated antibiotic resistance genes (ARGs) have emerged as critical waterborne contaminants, posing serious public health risks. This study proposes a disinfection strategy through sunlight powered calcium peroxide (CaO) treatment that simultaneously inactivates ARB and degrades ARGs in aquatic environments. Solar irradiation combined with CaO (3.

Pressure Treatment Induces Structural Symmetry Breaking to Narrow the Bandgap and Enhance Emission in UiO-66.

Metal-organic frameworks (MOFs), with a tunable porous structure and unique optical properties, exhibit excellent application potential in photocatalysis, fluorescent sensing, analytical detection, and biomedicine. Among numerous MOFs, UiO-66 is widely studied for its remarkable thermal and chemical stability, yet its optical performance is severely limited by a large bandgap and low charge transfer efficiency. This study proposes a pressure treatment strategy to regulate the conformation of octahedral secondary building units (SBUs) in UiO-66.

MoMtg1 Acts as a Novel Transcriptional Repressor of MoSwi6 During Appressorium-Mediated Penetration in the Rice Blast Fungus.

Appressoria are specialized penetration structures for many plant pathogenic fungi, including the rice blast fungus Magnaporthe oryzae, which evolves a set of complicated regulatory mechanisms to control appressorium development and function. Cell cycle control is essential for appressorium-mediated penetration, but the mechanism underlying its role remains largely elusive. Here, a conserved protein MoMtg1 is identified in filamentous fungi as a novel transcriptional repressor that plays a crucial role in cell cycle regulation.

Photothermally Responsive Biomimetic Composite Scaffolds Based on Polydopamine-Functionalized Nanoparticles/Polyurethane for Bone Repair.

In this study, a shape-changeable 3D scaffold with photothermal effects was developed to address the clinical challenges of complex bone defects. The multifunctional construct was fabricated via in situ polymerization combined with a gas foaming technique, creating hierarchical porous architectures that mimic the native bone extracellular matrix. By incorporating polydopamine (PDA)-modified amorphous calcium phosphate (CA) into poly(propylene glycol) (PPG)- and poly(ԑ-caprolactone) (PCL)-based polyurethane (PU).

Risk factors for pneumonitis after the combination treatment of immune checkpoint inhibitors and thoracic radiotherapy.

Background: The combination of immune checkpoint inhibitors (ICIs) and thoracic radiotherapy (TRT) has played a significant role in the improvement of tumor therapy, but the increased incidence of pneumonitis has greatly limited its application. To identify potential intervention targets, we analyzed risk factors for pneumonitis after combination therapy with ICIs and TRT.

Methods: Overall, 335 patients who received TRT and ICI therapy concurrently or sequentially were included in our study.

Perirenal fat: a neglected fat depot shaping heterogeneity of obesity along with hepatic fat.

Objective: Obesity is a heterogeneous condition that leads to diverse cardiovascular and metabolic outcomes. This study aimed to identify the primary visceral fats contributing to metabolically unhealthy obesity and to investigate the characteristics of fat distribution associated with different obesity-related complications.

Methods: A retrospective analysis was conducted on fat-selective magnetic resonance images (MRI) from 319 individuals with a BMI ≥24 kg/m².

Comparative effects of three SARS-CoV-2 inactivation methods on cytokine detection using LEGENDplex™ bead-based immunoassays.

In virology-related studies, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is routine to inactivate body fluid samples carrying the virus to reduce the spread of the virus and guarantee the safety of biobankers and researchers. However, inactivation treatments may affect the molecular structure of proteins in biological samples, and it is necessary to select an inactivation method that has the least impact on the target molecule associated with protein detection techniques. Cytometric Bead Array (CBA), a novel and powerful technology, allows the simultaneous quantification of up to 10-30 different soluble proteins from one sample, with a particular focus on various cytokines and chemokines in human body fluids.

Unraveling the Origin of Atmospheric Soluble Iron during Asian Dust Storms.

East Asian dust storms are a critical source of iron (Fe) to the North Pacific Ocean, where soluble Fe (Fe) supports marine primary productivity, playing an important role in global carbon cycling and ultimately influencing the climate. However, the relative contributions of natural mineral dust versus those of anthropogenic sources to Fe remain unclear. In this study, stable isotope composition (δFe) of total Fe (Fe) and Fe, together with mineralogical (STEM) and geochemical analysis for PM and PM size fractions of atmospheric particles collected during two dust storm events in Tianjin (China) were performed, with a goal to elaborate dynamics of Fe in dust storms and trace their sources.

Self-assembled DNAzyme based on the nicking enzyme amplification reaction for microRNA detection.

MiRNAs play crucial roles in cell proliferation, metabolism, and signal transduction, and have been established as biomarkers for cancer diagnosis and treatment for many years. Traditional methods for detecting miRNAs have several drawbacks, including poor sensitivity, time-consuming processes, and laborious steps. We have combined nicking enzyme amplification reaction (NEAR) with DNAzyme to develop a single-tube detection platform for highly sensitive and rapid detection of miRNA-21.

Case Report: A heterozygous mutation of NLRP3 in a Chinese child with NLRP3-AID.

Background: NLR family pyrin domain containing 3 (NLRP3)-associated autoinflammatory disease (NLRP3-AID), formerly known as cryopyrin-associated periodic syndrome, is a group of AIDs comprising neonatal-onset multisystem inflammatory disorder, Muckle-Wells syndrome, and familial cold autoinflammatory syndrome. Mutations in the NLRP3 gene are considered central to its pathogenesis.

Case Report: Here, we present a Chinese infant diagnosed with severe NLRP3-AID who carried a heterozygous variant in the NLRP3 gene.

Investigating the influence of atomic-scale self-limiting stress in CuAg@NiAg nanoparticle on CO electroreduction performance.

Whether stress at the micro-structural level exhibited self-limiting characteristics similar to macroscopic conditions, thus affecting the performance of catalysts in relation to the scope of stress influence, remained a topic requiring further investigation. In this study, we designed a CuAg@NiAg core-shell structure to induce lattice expansion and generate stress at the core-shell interface through the simultaneous action of displacement and co-reduction reactions. By adjusting the shell thickness, we investigated the impact of atomic-scale stress influence range (self-limiting behavior) on catalytic performance.