Surface soft patch-mediated supramolecular co-assembly of lysozyme/konjac glucomannan with optimized antimicrobial performance.

Assembly is a natural process where molecules spontaneously form ordered structures with specific functions. In supramolecular co-assembly, interactions between surface soft patches drive structural changes, though this key aspect is often overlooked. In this study, we propose a strategy for supramolecular co-assembly mediated by surface soft patches and apply this approach to optimize the broad-spectrum antibacterial activity of lysozyme (LYS)-konjac glucomannan (KGM) supramolecular co-assembly.

LYVAC/PDZD8 is a lysosomal vacuolator.

Lysosomal vacuolation is commonly found in many pathophysiological conditions, but its molecular mechanisms and functions remain largely unknown. Here, we show that the endoplasmic reticulum (ER)-anchored lipid transfer protein PDZ domain-containing 8 (PDZD8), which we propose to be renamed as lysosomal vacuolator (LYVAC), is a general mediator of lysosomal vacuolation. Using human cell lines, we found that diverse lysosomal vacuolation inducers converged on lysosomal osmotic stress, triggering LYVAC recruitment through multivalent interactions.

miR-7002-5p targets caspase-1 to suppress the inflammatory response of macrophages induced by Streptococcus equi subsp. zooepidemicus.

Streptococcus equi subsp. zooepidemicus (SEZ) is an important zoonotic pathogen that causes severe inflammatory diseases in various animal species. The host inflammatory response is a key factor in SEZ pathogenesis, yet the regulatory role of microRNAs (miRNAs) in this process remains largely unexplored.

Transcriptomic Analysis of the Heart Tissue in Spontaneously Hypertensive Rats Exposed to Acute Hypobaric Hypoxia.

We evaluated the effect of acute hypobaric hypoxia (AHH) on the heart of early-stage spontaneously hypertensive male rats (SHRs). The rats were classified into a control (ground level; ~400 m altitude) group and an SHR-AHH experimental group placed in an animal hypobaric chamber at a simulated altitude of 5500 m for 24 hours (24h).RNA-Seq analysis of the hearts showed that differentially expressed genes (DEGs) were primarily associated with cellular processes, binding, and cell parts.

Ultrasensitive, highly stretchable, self-healing and robust quaternized chitosan-based conductive hydrogel for wearable sensors.

Conductive hydrogels are widely used in electronic skin and wearable sensors. Their inherent self-adhesion, exceptional sensitivity, and remarkable toughness guarantee the consistent and reliable operation of flexible motion sensors. However, creating chitosan-based conductive hydrogels with all these properties remains challenging.

Atomic-Level Asymmetric Regulation of Co-NS Catalysts Accelerates Polysulfide Trapping and Conversion in Lithium-Sulfur Batteries.

Lithium-sulfur (Li-S) batteries are severely limited by the shuttling behavior of soluble lithium polysulfides (LiPSs) and slow catalytic conversion kinetics. Herein, a single-atom catalyst featuring asymmetric S-Co-N coordination (Co-SNC) supported by hollow carbon nanoboxes is designed to act as an efficient host catalyst of the Li-S battery. Experimental and theoretical calculations reveal that the introduction of S into the Co single-atom catalyst induces asymmetric local charge distribution around Co centers and more unpaired electrons.

Mie-Rayleigh-Raman-Doppler lidar system designed for multiple atmospheric parameter measurements in the troposphere and lower stratosphere at Haikou (19.9°N, 110.3°E).

In this study, a Mie-Rayleigh-Raman-Doppler lidar system featuring dual-wavelength (1064/532 nm) emission and 5-channel (1064, 532, 607, and 660 nm) reception was developed by employing various techniques, including seed injection narrowband pulse laser emission, frequency stabilization and discrimination, high-sensitivity photon counting, and precise wavelength separation. Lidar-received atmospheric Mie-Rayleigh scattering signals (@1064 nm, 532 nm) and Raman scattering signals (@607 nm, 660 nm) were precisely separated and extracted with a designed polychromator. The frequency stabilization/discrimination techniques with iodine molecular spectroscopy were utilized to identify the Doppler shift, and the winds in three different directions were successively detected by adopting a synchronization design of laser transmitting and backscattered signal receiving.

Mendelian randomization analysis with the GEO database: Exploring the molecular mechanism underlying insulin therapy for perioperative neurocognitive disorders.

Central insulin resistance is a significant factor in perioperative neurocognitive disorders (PND), yet the therapeutic effects and underlying molecular mechanisms of insulin remain unclear. We conducted a bidirectional Mendelian randomization (MR) study to investigate the causal relationships between insulin use and PND. The forward MR analysis revealed that insulin use considerably reduced the risk of delirium (odds ratio [OR] = 0.

General Approach to Fe(II/III)-Terpyridine Coordination Polymer Gel Induced by X═O Compounds for Enhanced Photocatalytic CO Reduction in the Solid-Gas Phase.

Herein, a chemical stimulation method to generate Fe-coordination polymer gels () by adding X═O (X = C, N, S, P) compounds to complex solutions has been reported. During the gelation process, Fe is reduced in situ to Fe, resulting in the formation of a metal-organic gel that incorporates both Fe and Fe. Leveraging this versatile technique, a series of were achieved across diverse chemical stimuli.

Magnolol inhibits viral replication and enhances antiviral immune responses against porcine reproductive and respiratory syndrome virus (PRRSV) in Marc-145 cells.

BackgroundPorcine reproductive and respiratory syndrome virus (PRRSV) is a pathogen that affects swine and causes substantial economic losses in the global pig industry. Despite the availability of vaccines, it remains crucial to explore innovative therapeutic strategies to control PRRSV infections. Magnolol, a bioactive compound extracted from the root and bark of , has demonstrated broad-spectrum antiviral activity in previous studies.

Cellulase activity and age-based variation of intestinal microbiota in Hezuo pigs.

Introduction: To examine the fiber-degrading enzyme activity and the changes of intestinal microbiota in Hezuo pigs across different age stages.

Methods: Fecal samples from 36 semi-grazed Hezuo pigs across five growth stages were collected for cellulase activity and 16S rRNA gene sequencing analysis.

Results: The results showed that the cellobiase activity in adult Hezuo pigs was markedly higher than in other groups ( < 0.

Novel Cyano-Artemisinin Dimer ZQJ29 Targets PARP1 to Induce Ferroptosis in Pancreatic Cancer Treatment.

Pancreatic cancer remains one of the most lethal malignancies in the digestive system, with limited available drugs and a need for improved efficacy. This unmet clinical need highlights the urgency to discover novel, highly efficient small-molecule compounds. Herein, a novel cyano-containing artemisinin dimer derivative, ZQJ29, is synthesized through structural modifications of artemisinin.

METTL3 mediates atheroprone flow-induced glycolysis in endothelial cells.

Atheroprone flow-increased glycolysis in vascular endothelial cells (ECs) is pivotal in EC dysfunction and the initiation of atherosclerosis. Methyltransferase 3 (METTL3) is a major mA methyltransferase for RNA N6-mehtyladenosine (mA) modifications to regulate epitranscriptome and cellular functions. With the atheroprone flow upregulating METTL3 and mA RNA hypermethylation, we investigate the role of METTL3 in atheroprone flow-induced glycolysis in ECs in vitro and in vivo.

Neferine inhibits PRRSV infection by disrupting p65 nuclear translocation.

Porcine reproductive and respiratory syndrome (PRRS), caused by the PRRS virus (PRRSV), imposes substantial economic losses on the global swine industry due to its profound impact on pig health and production efficiency. The main clinical manifestations are reproductive disorders such as miscarriage, stillbirth or weak foetuses in sows, as well as severe respiratory diseases in pigs of all ages. Currently, due to the high genomic diversity, antigen heterogeneity, and antibody-dependent enhancement effects of PRRSV, vaccination strategies cannot effectively control PRRSV infection.

Acute hypobaric hypoxia attenuates the anti-fatigue effects of pitolisant by downregulating the expression of organic cation transporter 1 and P-glycoprotein.

Objective: This study investigates the effects of acute hypobaric hypoxia (HH) on the anti-fatigue properties of pitolisant and explores the underlying mechanisms. The aim is to provide a theoretical basis for expanding its clinical indications and optimizing its use in individuals exposed to HH conditions.

Methods: The anti-fatigue effects of pitolisant were evaluated using the water maze, novel object recognition, and rotating rod tests.

Evaluation of the synergistic change in cultivated land and wetland in northeast China from 1990 to 2035.

Wetlands are the most biodiverse ecological landscape in nature and one of the most important natural resources for human beings. In recent years, wetlands in northeast China have been increasingly converted into cultivated land, resulting in significant reduction in wetland area. Currently, the extensive and prolonged use of natural resources, combined with mismanagement and climate change, presents considerable challenges for both governments and future sustainability.

Evaluation of drug-drug interaction between Suraxavir Marboxil (GP681) and itraconazole, and assessment of the impact of gene polymorphism.

Introduction: Suraxavir Marboxil (GP681) is a prodrug metabolized to GP1707D07, which inhibits influenza viral replication by targeting cap-dependent endonuclease through a single oral dose. This study assesses the in vivo drug-drug interaction (DDI) potential between GP681 (including its major metabolite GP1707D07, a substrate of CYP3A4) and itraconazole in healthy Chinese subjects, along with the safety profiles during co-administration. Additionally, it evaluates the impact of CYP1A2, CYP2C19, and CYP3A4 gene polymorphisms on GP1707D07 metabolism.

Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks.

Fluorescent biosensors have advanced biomedical research by enabling direct live-cell measurements of signaling activities. However, current technology offers limited resolution and dimensionality, impeding our ability to resolve and interrogate spatiotemporally regulated networks of signaling activities. Here we introduce highly sensitive chemigenetic kinase activity biosensors that combine the genetically encodable self-labeling tag, HaloTag7, with far-red-emitting synthetic fluorophores.

Deep Learning Based on Ultrasound Images Differentiates Parotid Gland Pleomorphic Adenomas and Warthin Tumors.

Exploring the clinical significance of employing deep learning methodologies on ultrasound images for the development of an automated model to accurately identify pleomorphic adenomas and Warthin tumors in salivary glands. A retrospective study was conducted on 91 patients who underwent ultrasonography examinations between January 2016 and December 2023 and were subsequently diagnosed with pleomorphic adenoma or Warthin's tumor based on postoperative pathological findings. A total of 526 ultrasonography images were collected for analysis.

Quercetin protects against neuronal toxicity by activating the PI3K/Akt/GSK-3β pathway in vivo models of MPTP-induced Parkinson's disease.

Background: Quercetin is a flavonoid commonly found in various fruits, vegetables, and grains. Studies have demonstrated that quercetin may help protect neuronal cells from damage caused by neurotoxins associated with Parkinson's disease, however, the underlying mechanism remains unclear.

Aim: The current study aimed to investigate the neuroprotective effects of quercetin in MPTP-induced Parkinson's disease mouse models and elucidate its mechanistic role in modulating the PI3K/Akt/GSK-3β signaling pathway.

Safety and Efficacy of Endoscopic Retrograde Appendicitis Therapy for Pediatric Patients: A Single-Center Retrospective Study in Chinese Children.

Introduction: Endoscopic retrograde appendicitis therapy (ERAT) is a new and noninvasive endoscopic technique originally developed by Liu in China for the management of acute appendicitis. Although its safety and efficacy have been extensively validated in adult populations, evidence regarding its use in pediatric patients remains scarce. The aim of this study was to evaluate the clinical outcomes of ERAT in pediatric appendicitis.

Smallholder farmers can achieve more sustainable wheat production through Consolidating Land for Uniform Practice.

Introduction: Land fragmentation of smallholder agriculture significantly constrains the adoption rate of optimal management practices and sustainable crop production.

Methods: We developed and implemented an innovative management model known as Consolidating Land for Uniform Practice (CLUP), which aimed to foster multi-actor collaboration and facilitate the large-scale application of optimal practices without altering land ownership. CLUP was implemented in wheat fields in the North China Plain for three consecutive years.

Caspase-1 is critical for mice in the defense against Streptococcus equi subsp. zooepidemicus infection by promoting macrophage phagocytosis.

Streptococcus equi subsp. zooepidemicus (SEZ) is an important pathogen which is responsible for a wide range of diseases in various species. Macrophages are professional phagocytes that can engulf microorganisms and trigger responses leading to microbial death.

Particulate matter pollution alters the bacterial community structure on the human skin with enriching the Acinetobacter and Pseudomonas.

Particulate matter (PM) has been recognized as a significant environmental contaminant with substantial effects on human health, although the impact of PM pollution on the skin microbiota is less understood. In this study, 78 skin microbiota samples from volunteers were obtained during periods of haze and non-haze in the spring and winter. The diversity, composition, and co-occurrence networks of the skin bacterial community were revealed using high-throughput sequencing.