Epidermal Growth Factor Receptor Activation Stimulates SOX9 Expression in Injured Renal Proximal Tubule Epithelial Cells.

Background: In surviving renal proximal tubule cells (RPTCs) following acute kidney injury (AKI), the induction of SOX9 expression plays a crucial role in promoting kidney repair. However, persistent upregulation of SOX9 in RPTCs contributes to the development of chronic kidney disease (CKD). The molecular mechanisms underlying SOX9 induction in response to kidney injury are not completely understood.

KCC3 is not required for the distal convoluted tubule response to reduced dietary potassium intake.

The distal convoluted tubule (DCT) plays a crucial role in potassium (K⁺) homeostasis, with electrogenic basolateral K⁺ flux well established as a regulator of its function. While the involvement of electroneutral basolateral K⁺ transport has been hypothesized, its precise role remains unclear. The electroneutral potassium chloride (Cl⁻) cotransporter, KCC3, is expressed in the kidney, but its role in DCT function has yet to be fully defined.

MoMtg1 Acts as a Novel Transcriptional Repressor of MoSwi6 During Appressorium-Mediated Penetration in the Rice Blast Fungus.

Appressoria are specialized penetration structures for many plant pathogenic fungi, including the rice blast fungus Magnaporthe oryzae, which evolves a set of complicated regulatory mechanisms to control appressorium development and function. Cell cycle control is essential for appressorium-mediated penetration, but the mechanism underlying its role remains largely elusive. Here, a conserved protein MoMtg1 is identified in filamentous fungi as a novel transcriptional repressor that plays a crucial role in cell cycle regulation.

Dehydration promotes intracellular lipid synthesis and accumulation.

Lipids can be considered a water reservoir used to offset dehydration stress as their oxidation by the mitochondria generates water. However, whether dehydration directly regulates lipid synthesis is unknown, which is the focus of this study. We found that dehydration stress decreases cellular oxygen consumption, increases intracellular lipid synthesis, and favors glutamine oxidation as a carbon precursor for lipid synthesis via remodeling mitochondrial metabolism.

Detection rate of cataract-related gene variants in sporadic childhood cataract patients in Southern China.

Objective: To screen for disease-associated variants in the cataract-related genes in Chinese sporadic childhood cataract patients by whole exome sequencing analysis.

Design: Prospective cohort study.

Subjects: Total 145 sporadic childhood cataract patients from southern China were recruited.

Broad-spectrum antifungal activity of aniline-modified tavaborole derivatives: insights from molecular docking and bioassays.

Background: Phytopathogenic fungi pose a severe threat to global agriculture, and the emergence of drug-resistant strains underscores the urgent need for novel fungicides. Tavaborole (AN2690), a clinically approved benzoxaborole antifungal, suppresses fungal growth by selectively targeting leucyl-tRNA synthetase (LeuRS) through covalent binding-a unique mode of action. In this study, we designed and synthesized a series of novel tavaborole derivatives featuring substituted aniline moieties.

Controversies, Consensuses, and Guidelines on Preventing, Diagnosing and Managing Acute-onset Bacterial Endophthalmitis after Cataract Surgery by the Academy of Asia-Pacific Professors of Ophthalmology (AAPPO), the Asia-Pacific Vitreo-retina Society (APVRS), and the Asia-Pacific Society of Ocular Inflammation and Infection (APSOII).

Post-cataract surgery endophthalmitis is a serious but largely preventable clinical entity. The implementation of uniform pre-operative, intra-operative, and post-operative protocols is essential to reduce its incidence. In light of emerging evidence and considerable variability in clinical practices, a panel of international experts from the Academy of Asia-Pacific Professors of Ophthalmology (AAPPO), the Asia-Pacific Vitreo-retina Society (APVRS), and the Asia-Pacific Society of Ocular Inflammation and Infection (APSOII) convened to develop evidence-based guidelines addressing all phases of cataract surgery.

Comparison of the Corneal High-Order Aberration and Relative Corneal Refractive Power Distribution Induced by Different Orthokeratology Lenses Designs.

Purpose: This study compared the myopia control effects of corneal high-order aberrations (CHOA) from two orthokeratology (OK) lenses based on their relative corneal refractive power shifts.

Methods: In this prospective cohort study, 101 school children with low to moderate myopia were divided into three groups: group A (N=35) used 3-curve design lenses, group B (N=31) used 4-curve design lenses, and group C (N=35) wore regular spectacles. Cycloplegic refraction, visual acuity, and axial length (AL) were measured at baseline and after one year.

DDGP-based chemoradiotherapy versus radiotherapy alone in newly diagnosed early-stage extranodal NK/T-cell lymphoma: A single-center retrospective analysis.

To determine the optimal treatment for early-stage extranodal NK/T-cell lymphoma (ENKTL), the efficacy differences between chemoradiotherapy (CRT) and radiotherapy (RT) alone, and between sequential DDGP (dexamethasone, cisplatin, gemcitabine, and pegaspargase) followed by RT (DDGP+RT) versus RT followed by DDGP (RT+DDGP) were investigated. This study compared RT alone versus CRT using DDGP regimen in 189 early-stage ENKTL patients. CRT showed higher objective response rates (95.

Guiding the geometry of graphene nanoribbon heterojunction via surface-adsorbed 10,10'-dibromo-9,9'-bianthryl.

Using 10,10'-dibromo-9,9'-biphenanthryl (DBBA) as a precursor, graphene nanoribbon (GNR) heterojunctions were synthesized on metal surfaces. On the Cu(110) surface, the strong interaction between DBBA molecules and the substrate limited the reaction pathway, resulting exclusively in the formation of graphene quantum dots via dehydrogenative cyclization. On Au(111), DBBA molecules underwent polymerization at elevated temperatures, yielding 7-atom-wide armchair GNRs (7aGNRs).

Screening of Potential Drug Targets Based on the Genome-Scale Metabolic Network Model of .

is a pathogenic bacterium widely distributed in marine environments, posing significant threats to aquatic organisms and human health. The overuse and misuse of antibiotics has led to the development of multidrug- and pan-resistant strains. There is an urgent need for novel antibacterial therapies with innovative mechanisms of action.

Thyrotoxic effects of organophosphate insecticides in adults: a bibliometric and meta-analysis.

Objective: Organophosphate insecticides (OPIs) are widely used worldwide, raising growing concerns over their potential thyrotoxic effects. Despite mounting evidence, inconsistencies persist regarding their impact on thyroid hormone (TH) regulation in humans. This study aimed to clarify the relationship between OPI exposure and TH levels in adults, addressing a critical gap in environmental health research.

Tear lactate improves the evaluation of proliferative diabetic retinopathy in type-2 diabetes patients.

Proliferative diabetic retinopathy (PDR) is the advanced stage of DR and characterized by retinal neovascularization (RNV). The diagnosis of PDR relies primarily on imaging features and blood glucose levels. Whether early biomarkers in other biofluid applied in the evaluation of PDR and RNV remain elusive.

Controversies, consensuses and guidelines on modern cataract surgery by the academy of Asia-Pacific professors of ophthalmology (AAPPO).

Phacoemulsification has revolutionized cataract surgery since its introduction by Charles Kelman in 1967, becoming the gold standard for cataract removal in most parts of the world. Despite its widespread adoption and high success rates, there exist controversies within the ophthalmic community regarding various aspects of the procedure. An international panel comprising 25 experts from 18 countries/territories was established by the Academy of Asia-Pacific Professors of Ophthalmology (AAPPO) to work on this consensus project.

[Clinical Features, Prognostic Analysis and Predictive Model Construction of Central Nervous System Invasion in Peripheral T-Cell Lymphoma].

Objective: To investigate the clinical features and prognosis of central nervous system (CNS) invasion in peripheral T-cell lymphoma (PTCL) and construct a risk prediction model for CNS invasion.

Methods: Clinical data of 395 patients with PTCL diagnosed and treated in the First Affiliated Hospital of Zhengzhou University from 1st January 2013 to 31st December 2022 were analyzed retrospectively.

Results: The median follow-up time of 395 PTCL patients was 24(1-143) months.

Early assessment of therapeutic efficacy in lymphoma patients via a blood-based multi-omics response monitoring test.

Background: Periodic fluorodeoxyglucose (FDG) positron emission tomography (PET)-computed tomography (CT)/CT-based response examinations are the current standard for clinical assessment of lymphoma treatment response. In this prospective study, we applied a blood-based multi-omics test, SeekInClarity, to assess treatment response and to predict therapeutic outcomes in the major lymphoma subtypes.

Methods: We prospectively recruited 116 lymphoma patients from two clinical centers, and collected blood samples at pre-treatment (baseline) and after two treatment cycles (landmark) to calculate molecular tumor burden (MTB) score using SeekInClarity.

Faecalibacterium prausnitzii promotes anti-PD-L1 efficacy in natural killer/T-cell lymphoma by enhancing antitumor immunity.

Background: Natural killer/T-cell lymphoma (NKTCL) is a highly aggressive subtype of lymphoma characterized by a poor prognosis. While immune checkpoint blockade (ICB) therapy has emerged as an effective treatment modality for various cancers, its efficacy among individuals is inconsistent and remains suboptimal for the majority of NKTCL patients. Faecalibacterium prausnitzii, recognized as a next-generation probiotic with immunomodulatory capabilities, has not yet been fully explored for its potential to influence the outcomes of ICB therapy.

Antifungal Activity and Mechanism of Novel Benzimidazole-Containing Flavonol Derivatives as Potential Tubulin Polymerization Inhibitors against .

Microtubules are essential components of cells, contributing to the maintenance of cell shape, intracellular transport, cell division, signal transduction, and various other functions. To discover novel tubulin-targeting fungicides, two series of benzimidazole-containing flavonol derivatives were designed, synthesized, and evaluated for their antifungal activity. Compound exhibited optimal antifungal activity against (EC = 0.

YTHDF2 regulates the adriamycin (ADM) resistance in NKTCL by modulating the m6A modification of SLC16A9.

Context: Natural killer/T-cell lymphoma (NKTCL) is an aggressive malignancy with a high propensity for drug resistance, particularly to anthracyclines like adriamycin (ADM). The molecular mechanisms of ADM resistance in NKTCL are not fully understood.

Objective: In this study, we aimed to elucidate the role of YTHDF2 in the regulation of SLC16A9 mRNA stability and its implications in ADM resistance in NKTCL.

Clinical features and prognostic factors of intravascular large B-cell lymphoma: a cohort study of 20 patients from 2018 to 2024.

Background: Intravascular large B-cell lymphoma (IVLBCL) is a rare, aggressive lymphoma characterized by lymphoma cells proliferating within small blood vessels, lacking an extravascular mass. Its low incidence complicates diagnosis and treatment.

Methods: This study analyzed 20 IVLBCL patients diagnosed between 2018 and 2024, aiming to describe their clinical presentations, diagnostic procedures, treatment, and outcomes.

Risk of gestational diabetes mellitus in relation to serum metal mixtures and mediating effects of metabolites in Chinese population: A dual approach using bibliometric and epidemiological analyses.

Gestational diabetes mellitus (GDM) is a common complication of pregnancy, posing obstetrical and metabolic risks. While emerging evidence suggests an association between specific metal exposure and GDM, the crucial metabolic changes between serum metal exposure and GDM risk have yet to be fully clarified. Here we employed a combination of bibliometric and epidemiological methodologies to investigate the relationship between metal exposure and GDM and potential mediation role of metabolites in Chinese pregnant women.

BaSbBS: a record-high-performance birefringent crystal identified by a target-driven closed-loop strategy.

Exploring infrared (IR) birefringent materials with both large birefringence (Δ) and wide band gaps ( ) is urgently demanded for high-power optoelectronic applications and has long been a tough challenge due to the intrinsic contradictory relationship between the two metrics. Herein, we developed a target-driven closed-loop framework in coupling with functional motif and crystal structure screening, deep learning assisted high-throughput optical property computation, targeted experiment and mechanism investigation, enabling efficient discovery of potential birefringent materials. Utilizing it, a batch of superior IR birefringent crystals containing planar [BS] and/or stereochemically active lone pair (SCALP) groups ([SbS], [SnS], ) were identified: six with huge birefringence (Δ > 1.

Lipid metabolism disorder promoting retinal structural and functional damage in ApoE mice with age superposition.

This study aimed to establish a model of abnormal lipid metabolism in Apolipoprotein E (ApoE) knockout mice by feeding them a high-fat diet (HFD) and to investigate the impact of this abnormal lipid metabolism on retinal blood perfusion, structure, and function, particularly the retinal ganglion cell (RGC). Both HFD and regular diet (RD) feeding were conducted in C57BL/6J mice and ApoE mice. Lipid metabolism was assessed using hematoxylin-eosin (HE) staining, oil red staining, and blood lipid detection.

Development and validation of a 3-D deep learning system for diabetic macular oedema classification on optical coherence tomography images.

Objectives: To develop and validate an automated diabetic macular oedema (DME) classification system based on the images from different three-dimensional optical coherence tomography (3-D OCT) devices.

Design: A multicentre, platform-based development study using retrospective and cross-sectional data. Data were subjected to a two-level grading system by trained graders and a retina specialist, and categorised into three types: no DME, non-centre-involved DME and centre-involved DME (CI-DME).

The small molecule drug CBL0137 interferes with DNA damage repair and enhances the sensitivity of NK/T-Cell lymphoma to cisplatin.

This study aimed to investigate the in vitro and in vivo antitumor effects and mechanisms of the small molecule anticancer drug CBL0137 in NK/T-cell lymphoma (NKTCL), as well as its efficacy when combined with chemotherapy or immunotherapy. Cell viability assays were performed to evaluate the inhibitory effect of CBL0137 on NKTCL cell proliferation in vitro. Flow cytometry was used to assess the effects of the drug on apoptosis and cell cycle progression.