Effect of pressure biofeedback training combined with Liuzijue Qigong training on respiratory function improvement in post-stroke patients with tracheotomy and decannulation: study protocol for a randomised controlled trial.

Introduction: Stroke causes neurological deficits and respiratory dysfunction, with prolonged bed rest exacerbating secondary pulmonary injury. This study evaluated the efficacy of pressure biofeedback training combined with Liuzijue Qigong (LQG) in improving functional outcomes and respiratory function in patients with tracheostomised stroke.

Methods And Analysis: This will be a parallel, single-centre randomised controlled trial involving 66 patients.

PLEKHF1 Induces Mitochondrial Dysfunction to Inhibit Osteosarcoma Growth and Metastasis.

Osteosarcoma (OS) is an uncommon malignancy with stagnant survival rates over the past four decades and early-stage metastasis, predominantly affecting children and adolescents. This study identified significant metabolic differences between metastatic and non-metastatic OS samples through bioinformatics analysis, highlighting key processes such as cell proliferation, mitochondrial assembly, and changes in mitochondrial membrane permeability. Among differentially expressed genes, Pleckstrin Homology And FYVE Domain Containing 1 (PLEKHF1) was the most significantly downregulated in metastatic OS samples.

Advances in the prevention and management of postoperative bleeding complications in pancreaticoduodenectomy: Current strategies and future directions precise.

Postoperative bleeding (POB) is a major complication following pancreaticoduodenectomy (PD), leading to significant morbidity and potential mortality. This minireview focuses on the prevention and management strategies for POB, synthesizing current evidence on surgical techniques, perioperative management, and postoperative interventions. Effective prevention strategies include the use of regional vessel wrapping, optimal pancreatic anastomosis, and meticulous intraoperative hemostasis.

PSD3 as a context-dependent modulator of immune landscape and tumor aggressiveness in esophageal squamous cell carcinoma.

In this study, we investigated PSD3, CD274 (PD-L1), and TNFSF18 as potential immune-related biomarkers in esophageal squamous cell carcinoma (ESCC) using integrative transcriptomic and experimental approaches. CD274 and TNFSF18 were consistently up-regulated in ESCC across both TCGA and GEO datasets, while PSD3 showed significantly higher expression in TCGA but no significant difference in the GEO cohort. Only PSD3 demonstrated a significant association with overall survival, with higher expression correlating with improved prognosis.

Combinatorial mutagenesis and fermentation optimization biotechnologies synergistically enhance monacolin K content in functional red yeast rice.

Introduction: Red yeast rice (RYR) is produced through solid-state fermentation by Monascus genus. Its functional component, Monacolin K (MK), has the same structure as lovastatin and can effectively inhibit HMG-CoA reductase, thereby reducing serum cholesterol.

Methods: A combinatorial mutagenesis strategy integrating atmospheric room-temperature plasma and heavy-ion radiation was employed to generate mutant strains.

The influence of college students' psychological resilience on problem solving: based on eye tracking technology.

Introduction: The digital intelligence era has put forward new requirements for cultivating innovative talent, and problem solving ability is one of the key abilities for the cultivation of innovative talents.

Methods: Using eye tracking technology, this study explored the impact of college students' psychological resilience on problem solving. A mixed experimental design (psychological resilience level × difficulty of questions) was employed, combining eye movement data, emotion observation record sheet, and retrospective oral reports.

Mitochondrial AOX Activation Drives Pyribencarb Resistance in : Cellular Trade-Offs and Nontarget Site Mechanisms.

In this study, pyribencarb-resistant mutants were generated through fungicide mutagenesis, and the parental isolate SBH1 and three representative mutants (RH1-1, RH1-2, and RH1-3) were selected for comparative analysis. All mutants displayed reduced mycelial growth, impaired sclerotia formation, decreased respiration, and mitochondrial dysfunction, with elevated cell membrane permeability. No differences were detected in sclerotia weight, oxalate levels, or pathogenicity relative to the parental isolate.

Chromatin dynamics of a large-sized genome provides insights into polyphenism and X0 dosage compensation of locusts.

Locusts are characterized by a large genome size, polyphenism and an X0 sex determination system. Here we generated chromosome-level genomes for both desert and migratory locusts, as well as a comprehensive chromatin map for the latter. We found that genome enlargement is associated with an increased number of enhancers in expanded intronic regions.

Research progress on NAT10-mediated acetylation in normal development and disease.

N4-acetylcytidine (ac4C) is an evolutionarily conserved RNA modification catalyzed by the acetyltransferase NAT10. It regulates RNA stability, translation, and post-transcriptional processes. Meanwhile, NAT10 functions as a dual-function enzyme exhibiting both protein acetyltransferase and RNA acetylase activities.

sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov. and sp. nov., isolated from ginseng rhizosphere.

Thirteen novel strains - J2L1-78, J2P1-59, J22R24, J22R133, J22P168, J22P271, J22R187, J22R193, J2L1-63, J2R1-6, J22G21, J22G47, and J31P216 - were isolated from the rhizosphere of 20-year-old ginseng in Jilin Province, P.R. China.

Nutrient-Enriched Germinated Brown Rice Alters the Intestinal Ecological Network by Regulating Lipid Metabolism in Rats.

Metabolic diseases such as high blood lipids, high blood sugar, and disrupted gut microbiota pose a serious threat to people's physical health. The occurrence of these diseases is closely related to the lack of nutrients in daily rice staple foods, but there is a lack of comprehensive analysis of the underlying mechanisms. This study used fully nutritious brown rice as raw material, and after germination under various stress conditions, it significantly increased the levels of gamma aminobutyric acid (GABA, four carbon non protein amino acid), resistant starch, flavonoids, and other components that regulate metabolic diseases.

Comparative Transcriptomic Analysis of the Liver and Spleen in Ussuri Catfish () Challenged with Polyriboinosinic Polyribocytidylic Acid (Poly(I:C)).

Poly (I:C), a viral mimic, is capable of activating the antiviral immune mechanisms in teleosts. In this study, we investigated the transcriptional responses of Ussuri Catfish () to poly (I:C) stimulation at 3 and 48 h, focusing on the similarities and differences in antiviral mechanisms exhibited in the liver and spleen. At 3 h, the signaling pathways that were concurrently enriched in both the spleen and liver include JAK-STAT, TNF, NF-κB, RIG-I-like receptor, and NOD-like receptor.

Multifaceted Role of RIMBP2 in Promoting Hearing in Murine Cochlear Hair Cells.

The mammalian cochlea relies on outer and inner hair cells (OHCs/IHCs) for sound amplification and signal transmission. Rab3-interacting molecular binding protein 2 (RIMBP2), expressed in receptor cells and neurons at synaptic active zones, remains poorly characterized in hearing. We therefore generated a Rimbp2 knockout (KO) mouse model (Rimbp2), which exhibited severe hearing loss with elevated thresholds, prolonged latencies, and reduced amplitudes in auditory brainstem response Wave I.

Anterior olfactory nucleus mediates parallel inter-bulbar pathways in rodents.

Background: Interhemispheric communication of olfactory information is crucial for accurate odor perception and odor source localization in animals. However, the underlying structural basis for this communication in mammals remains poorly understood. Using electrophysiological recordings and virus-mediated tracing, we systematically dissected the neural circuits involved in interhemispheric transmission between the bilateral olfactory bulbs (OBs).

Efficient activation of peracetic acid by Fe-doped g-CN for selective degradation of emerging contaminants: Unraveling the role of reactive complex.

Peracetic acid (PAA)-driven advanced oxidation processes (AOPs) are increasing favored for water treatment due to their environmentally benignity and ease of activation. However, the development of low-cost and high-performance activators is still a primary challenge. In this study, Fe-doped g-CN (Fe-N-C) was fabricated as an efficient peracetic acid (PAA) activator for sustainable degradation of emerging contaminants (ECs) like bisphenol A (BPA) with a removal efficiency of 97.

Biological functions and clinical implications of the CMPK2 across multisystemic diseases.

Cytidine/Uridine monophosphate kinase 2 (CMPK2) is a crucial enzyme responsible for the phosphorylation of nucleotides, specifically converting cytidine monophosphate (CMP) and uridine monophosphate (UMP) into their respective diphosphates, known as cytidine diphosphate (CDP) and uridine diphosphate (UDP). Recent studies have demonstrated that CMPK2 plays a pivotal role in multiple pathophysiological processes, such as cellular nucleotide metabolism, energy homeostasis, and inflammatory response. Dysregulation of CMPK2 has been implicated in a variety of pathologies, including viral infections, neurodegenerative diseases, and autoimmune disorders.

Perivascular inflammation in the progression of aortic aneurysms in Marfan syndrome.

Inflammation plays important roles in the pathogenesis of vascular diseases. We here show the involvement of perivascular inflammation in aortic dilatation of Marfan syndrome (MFS). In the aorta of MFS patients and Fbn1C1041G/+ mice, macrophages markedly accumulated in periaortic tissues with increased inflammatory cytokine expression.

Self-Enhanced Ratiometric ECL Strategy Based on a Dual-Emission Ce/Tb-MOF Luminophore with Spatially Resolved Coreactant Delivery for Ultrasensitive Pro-GRP Detection.

Dual-signal-output ratiometric electrochemiluminescence (ECL) platforms offer a promising solution to address the instability and inaccuracies associated with single-signal ECL detection. However, most existing ratiometric systems still rely on two independent luminophores, inevitably increasing the system complexity and compromising the detection accuracy. In this study, a bimetallic dual-ligand ECL luminophore, Ce/Tb-MOF, was synthesized via a solvothermal approach, exhibiting distinct anodic and cathodic ECL emissions from a single luminophore.

Deciphering the Potential Causal and Prognostic Relationships Between Gut Microbiota and Brain Tumors: Insights from Genetics Analysis and Machine Learning.

The concept of the microbiota-gut-brain axis has witnessed significant advancements, and observational studies revealed dysbiosis in the gut microbiota of patients with brain tumors. The causal relationship between gut microbiota and brain tumors and the potential prognostic value of microbiota are still unclear. Based on multiple Mendelian randomization analyses, this study confirms the causal effects of four gut microbes on meningioma, seven gut microbes on pituitary tumor, and eight gut microbes on glioma.

Effect of glycerol monostearate on the structure and digestibility of extruded debranched rice flour under extrusion.

Increasing amylose content in grain flours enhances its complexation with monoglycerides, leading to elevated resistant starch (RS) levels. This study investigated the effect of starch digestibility and structural characteristics in rice flour (RF) following primary pullulanase complex extrusion and secondary glycerol monostearate (MG) (2 %, 4 %, 6 %, and 8 %) complex extrusion. The EERF-MG exhibited a significant formation of V-type complexes with increased relative crystallinity compared to RF.

The clinical characteristics and diagnostic and treatment protocol for 14 acute Vibrio vulnificus infections caused by aquatic products.

Vibrio vulnificus infections caused by aquatic product-related injuries pose severe clinical challenges due to their rapid progression and high morbidity and mortality. Early diagnosis and timely intervention are critical to improving patient outcomes, yet standardized diagnostic and treatment protocols remain limited. We conducted a retrospective descriptive case series of 14 patients with confirmed V vulnificus infection admitted between 2020 and 2023.

A novel paclitaxel eluting bioresorbable vascular stent with a super flexible stent structure and round cross section struts fabricated using 3D printing technology with a rotating platform.

Bioresorbable stents (BRS) have emerged as a groundbreaking development in the field of percutaneous coronary intervention (PCI) as they address the long-standing concerns of metallic stents. Nevertheless, the observed higher thrombosis rates in the first generation BRS, i.e.

RPN1 Is Associated With Immunosuppression in Pan-Cancer and Affects the Malignant Phenotype of Tumor.

Ribophorin 1 (RPN1), a key component of the oligosaccharyltransferase complex, is implicated in tumor progression through glycosylation-mediated pathways, yet its pan-cancer roles remain unexplored. This study presents a comprehensive multi-omics analysis of RPN1 across 33 cancers such as sarcoma (SARC), integrating genomic, transcriptomic, and proteomic data from TCGA, GTEx, and CPTAC. RPN1 was significantly overexpressed in 14 malignancies and correlated with advanced tumor stages and poor prognosis in glioblastoma (GBM), lower-grade glioma, SARC and hepatocellular carcinoma, validated in an independent glioma cohort (n = 151).

High-Throughput Fabrication of Microscale Gold Nanoparticle Superstructures with Tunable Plasmonic Coupling for Ultrasensitive SERS Detection.

Noble metal nanostructures are attractive substrates for surface-enhanced Raman scattering (SERS) but face persistent challenges in combining efficient hot-spot engineering with scalable fabrication. This paper presents a high-throughput, hot-spot-designable approach for assembling gold nanoparticles (AuNPs) onto micrometer-scale, two-dimensional polymer single-crystal templates, producing ∼10 uniform AuNP assemblies per milliliter. The assemblies, with planar micrometer-scale dimensions, are fully compatible with commercial confocal Raman systems.

Pillarureaarenes: Urea-Embedded Pillararenes for Amino Acid Derivative Recognition.

A three-dimensional pillarureaarene () was synthesized via fragment-coupling cyclization of dialkoxybenzene and urea units. Single-crystal analysis revealed a shield-shaped structure stabilized by intramolecular hydrogen bonds. The macrocycle demonstrated strong binding with phenylalanine ethyl ester salts.