ROS and Drp1-mediated mitochondrial fission contributes to the hyposalivation caused by Sjögren's disease.

Background: Sjögren's disease (SjD) is an autoimmune disorder characterized by sicca syndrome with undetermined roles of reactive oxygen species (ROS) and mitochondrial dynamics in the damaged glands. This study aimed to clarify the roles of ROS and mitochondrial dynamics in SjD-related hyposalivation and explored the therapeutic effect of targeting mitochondrial fission to treat hyposalivation in murine SjD.

Methods: Single-cell sequencing analysis of submandibular glands (SMGs) from mice with SjD was performed.

CNO induced Ca store and glutamate-dependent nonspecific Ca signalling in DREADD-free brain slices.

DREADD (design receptors exclusively activated by designer drugs) is a widely used powerful tool designed to study specific cellular functions. However, off-target effects of chemogenetic activators, including clozapine N-oxide (CNO) and deschloroclozapine (DCZ), have been reported. In our study, we demonstrated the direct off-target effects of CNO and DCZ on basal Ca levels in the locus coeruleus nucleus in both neurons and astrocytes by combining viral microinjection, Ca imaging and electrophysiology.

Cigarette Tar Enhanced ECs Pyroptosis via CAMKII/Drp1/mtDNA: Novel Insight Into the Mechanism of Plaque Erosion.

Smoking is the only cardiovascular risk factor for plaque erosion. We found cigarette tar resulted in erosion-like lesion development in apolipoprotein E mice, with mural thrombosis, discontinuous endothelium, platelet activation, smooth muscle cell proliferation, and hyaluronic acid accumulation in the aorta. Single-cell transcriptomics revealed that genes relating to pyroptosis, platelet activation, and leukocytes adhesion were significantly increased in an endothelial cell subset.

Targeting KEAP1/NRF2 interaction with oleuropein ameliorates atherosclerosis by inhibiting macrophage ferroptosis.

Atherosclerosis (AS) is a chronic inflammatory arterial disease. Oleuropein (OL), extracted from olive leaves, has demonstrated broad cardioprotective properties. However, the effects of OL on AS remain to be fully elucidated.

Elimination of Intraspecific Competition Does Not Improve Maize Leaf Physiological and Biochemical Responses to Topsoil Degradation.

Soil degradation limits maize grain yield, but the mechanisms by which leaf functions respond to topsoil depth and their contributions to yield are unclear. We quantified the response mechanisms of leaf functions to topsoil depth with topsoil depths of 10 cm (S), 20 cm (S), 30 cm (S), 40 cm (S), and 50 cm (S) and planting densities of 15,000 plants ha (D, the plant spacing was 111.1 cm and there was no mutual influence between individuals) and 75,000 plants ha (D).

Nutrient-Enriched Germinated Brown Rice Alters the Intestinal Ecological Network by Regulating Lipid Metabolism in Rats.

Metabolic diseases such as high blood lipids, high blood sugar, and disrupted gut microbiota pose a serious threat to people's physical health. The occurrence of these diseases is closely related to the lack of nutrients in daily rice staple foods, but there is a lack of comprehensive analysis of the underlying mechanisms. This study used fully nutritious brown rice as raw material, and after germination under various stress conditions, it significantly increased the levels of gamma aminobutyric acid (GABA, four carbon non protein amino acid), resistant starch, flavonoids, and other components that regulate metabolic diseases.

The Research Hotspots and Future Trends of Adaptive Learning in the Age of Artificial Intelligence: A Bibliometric Analysis From 2014 to 2024.

To examine the research status and developmental trend within the adaptive learning. Adaptive learning provides personalized learning paths based on the unique needs of each learner. However, a comprehensive bibliometric analysis of this field remains limited.

TaWAKL8-2B, a wall-associated receptor-like kinase, mediates wheat rust resistance by linalool and ROS accumulation.

Wall-associated receptor kinases (WAKs) and WAK-likes (WAKLs) play pivotal roles in regulating plant immunity, through multiple downstream signaling components. However, knowledge of WAKs/WAKLs in wheat immune responses to rust diseases remain limited. In this study, we identified and characterized a wheat WAKL, TaWAKL8-2B, which is upregulated during wheat resistance to both Puccinia striiformis f.

Dual Bioconversion Strategy: Synergistic Germination and Fermentation Engineering for a γ-Aminobutyric Acid-Enriched Beverage from Brown Rice.

Growing demand for plant-based nutraceuticals drives the need for innovative bioprocessing strategies. This study developed an integrated approach combining germination and Lactobacillus-mediated fermentation to produce a γ-aminobutyric acid (GABA)-enriched functional beverage from brown rice. Systematic screening identified an optimal rice cultivar for germination.

Mediator effects of cognitive load on the relationship between task complexity and guideline adherence among clinical nurses: a cross-sectional survey of nurses in China.

Background: Suboptimal adherence to guidelines is a common phenomenon in clinical settings. Studies regarding the complexity of tasks, cognitive load, and adherence to guidelines among clinical nurses are noteworthy, but limited.

Purpose: To investigate whether cognitive load mediates the association between task complexity and guideline adherence in clinical nurses.

Hypercholesterolemia and the role of lipid metabolism gene CES1 in immune infiltration promote central nervous system relapse in acute myeloid leukemia.

Background: Alterations in multiple lipid metabolism pathways are associated with cancer progression. However, the relationship between lipid metabolism and central nervous system (CNS) relapse in acute myeloid leukemia (AML) remains unclear.

Methods: We conducted a retrospective analysis of 806 AML cases to evaluate the association between serum lipid levels and the risk of CNS relapse.

Effects of dietary supplementation with different levels of rose pomace on rumen bacterial diversity and fermentation, and longissimus dorsi fatty acids in Hu sheep.

To address the underutilization of rose processing byproducts and meet the demand for antibiotic-free meat production, this study examined the effects of dietary supplementation with rose pomace (RP) on growth performance, rumen fermentation, bacterial diversity, and longissimus dorsi amino acid and fatty acid profiles in Hu sheep. Forty male Hu sheep were randomly assigned to four groups ( = 10): a control group (RP0) with a basal diet and three treatment groups supplemented with 2, 4, or 8% RP (RP2, RP4, RP8). Results showed that RP8 had significantly higher dry matter intake (DMI) than RP2 ( ≤ 0.

Hyperuricemia in ob/ob mice relates to hepatocellular pyruvate metabolism/ xanthine oxidase axis.

Objective: The study aimed to examine the association between obesity and hyperuricemia in ob/ob mice.

Methods: An animal model of obesity was developed using male ob/ob mice. Biochemical parameter test kits were used to measure serum uric acid (UA), hepatic xanthine oxidase (XOD) activity, serum creatinine (Scr), serum lipid profiles, and blood urea nitrogen (BUN).

A multi-algorithm prognostic model combining inflammatory indices and surgical features in distal cholangiocarcinoma.

Background: Derived neutrophil-to-lymphocyte ratio (dNLR) is an emerging blood-based inflammatory biomarker previously reported to have prognostic value in various malignancies. This study aimed to investigate the prognostic significance of dNLR in patients with distal cholangiocarcinoma (dCCA) after curative resection.

Methods: Clinicopathological data of patients with dCCA in our hospital from Jan.

Single-dose GC101 gene therapy for spinal muscular atrophy types II and III: an open-label single-arm study.

QUICK TAKE.

Magnetic nanoporous carbon-based self-enriched and self-cleaning recyclable substrate Co/C@Ag/g-CN for SERS detection and photocatalytic degradation of malachite green.

This study reports the design and fabrication of a magnetic nanoporous carbon-based recyclable surface-enhanced Raman scattering (SERS) substrate Co/C@Ag/g-CN, which can simultaneously achieve SERS detection and visible-light photocatalytic degradation of malachite green (MG). Co/C and g-CN can spontaneously adsorb and concentrate MG near plasmonic nanoparticles, thereby enhancing the SERS response. The substrate exhibited a SERS enhancement factor of approximately 1.

Occupational stress and humanistic care characteristics among Chinese nurses: the mediating role of presenteeism.

Background: The occupational stress experienced by nurses and their humanistic care characteristics directly influence the quality of nursing. Presenteeism serves as an external manifestation of occupational stress and may diminish nurses' humanistic care capabilities. Therefore, the aim of this study was to explore the relationships among the occupational stress, presenteeism and humanistic care characteristics of nurses in China, as well as the mediating role of presenteeism.

Triple stem cell infusion alleviated graft-versus-host disease and improves outcomes in unmanipulated haploidentical hematopoietic stem cell transplantation.

Haploidentical hematopoietic stem cell transplantation (Haplo-HSCT) provides cure opportunity for patients requiring prompt allogeneic HSCT but failing to identify well-matched donor, but its outcomes are potentially impaired by increased transplant-related mortality (TRM). We performed haplo-HSCT using granulocyte colony-stimulating factor (G-CSF)-primed peripheral blood stem cells (PBSCs), umbilical cord mesenchymal stem cells (UC-MSCs) and third-party unrelated umbilical cord blood (UCB) stem cells. Modified "Beijing protocol" were performed in this study.

A discrete SIR epidemic model incorporating media impact, resource limitaions and threshold switching strategies.

The paper explores the effects of media influence and limited medical resources on the spread of infectious diseases using mathematical modeling. We construct a switching epidemic model that incorporates a media influence factor, an inoculation function, and a cure function. This model is subsequently discretized and studied via Euler's method.

Combining transcriptomics and network pharmacology to reveal the mechanisms of QuZhuoHuaYu decoction ameliorating gouty arthritis.

QuZhuoHuaYu decoction (QZHY), a traditional Chinese medicine, has demonstrated a potential effect in gouty arthritis (GA) treatment, but its underlying mechanisms have yet to be fully elucidated. In this study, a strategy of UHPLC-Q-Orbitrap-MS/MS, transcriptomics, and network pharmacology combined with experimental verification was conducted to explore the constituents and the potential mechanisms of QZHY. A total of 26 compounds were identified from QZHY.

Direct sequencing of DNA 5-methylcytosine by engineered dioxygenase NTET-assisted eNAPS.

DNA methylation (5-methylcytosine, 5mC) represents the most prevalent modification in mammals, which is closely linked to disease pathogenesis and cancer development. Single-base resolution sequencing and quantitative analysis of 5mC are essential for elucidating its biological functions. However, current methods are still limited by resolution, sequencing bias, and false positives.

Matrix stiffness regulates glucose-6-phosphate dehydrogenase expression to mediate sorafenib resistance in hepatocellular carcinoma through the ITGB1-PI3K/AKT pathway.

Sorafenib is an antiangiogenic and antiproliferative chemotherapeutic drug that plays a crucial role in the treatment of patients with advanced hepatocellular carcinoma (HCC). However, resistance to sorafenib greatly limits its therapeutic efficacy. This highlights the importance of determining the mechanisms underlying resistance to antiangiogenic therapy.

The Design of the NiN/NbN Heterostructure as Bifunctional Adsorption/Electrocatalytic Materials for Lithium-Sulfur Batteries.

Lithium-sulfur (Li-S) batteries are hindered by the sluggish electrochemical kinetics and poor reversibility of lithium polysulfides (LiPSs), which limits their practical energy density and cycle life. In order to address this issue, a novel NiN/NbN heterostructure was synthesized via electrospinning and nitridation as a functional coating for polypropylene (PP) separators. Adsorption experiments were conducted in order to ascertain the heterostructure's superior affinity for LiPSs, thereby effectively mitigating their shuttling.

Research Topics and Trends of Clinical Decision Support Systems in Intensive Care Units: A Bibliometric Analysis.

Background: Hospital administrators apply information technology to improve healthcare quality; however, a comprehensive bibliometric analysis remains limited.

Aim: To explore the research topic, key contributors and development trends of clinical decision support systems (CDSS) in intensive care units (ICUs).

Study Design: Between January 1, 2014, and December 31, 2024, a bibliometric analysis was undertaken.

Linperlisib Plus Chidamide in Relapsed or Refractory Cutaneous T-Cell Lymphoma: A Nonrandomized Clinical Trial.

Importance: Patients with relapsed or refractory (r/r) cutaneous T-cell lymphoma (CTCL) have limited treatment options. Combining agents that target complementary oncogenic pathways may enhance efficacy while maintaining tolerability.

Objective: To evaluate the safety and efficacy of linperlisib, a PI3Kδ inhibitor, combined with chidamide, a histone deacetylase inhibitor, in patients with r/r CTCL.