[Effect of 3D laparoscopic radical prostatectomy on urinary control and sexual function of patients with prostate cancer].

Objective： To investigate the effects of 3D laparoscopic radical resection prostatectomy(LRP) on urinary control and sexual function of patients with prostatic cancer. Methods： A total of 268 patients who were treated with LRP in the Fifth People's Hospital of Huai'an City from January 2019 to May 2022 were selected and divided into 2 groups according to surgical methods, with 134 cases in each group. The patients in the control group were treated with traditional LRP, and the 3D LRP was used in the observation group.

Terbium (III) and adenosine monophosphate coordinated hydrogel for fluoroquinolones sensing by combining machine learning.

Nondestructive identification of fluoroquinolones distribution on food is an approach to simplify detection steps and improve detection efficiency. In this study, a fluorescent sensor based on terbium (III) and adenosine monophosphate coordinated hydrogel (TbCHy) has been synthesized, which is assisted by machine learning for intelligent and nondestructive detection of fluoroquinolones (FQs) in meat. Norfloxacin (NOR) was used as the model target, the TbCHy-based sensor performed a good linearity when NOR concentration ranged from 0.

Mitochondrial AOX Activation Drives Pyribencarb Resistance in : Cellular Trade-Offs and Nontarget Site Mechanisms.

In this study, pyribencarb-resistant mutants were generated through fungicide mutagenesis, and the parental isolate SBH1 and three representative mutants (RH1-1, RH1-2, and RH1-3) were selected for comparative analysis. All mutants displayed reduced mycelial growth, impaired sclerotia formation, decreased respiration, and mitochondrial dysfunction, with elevated cell membrane permeability. No differences were detected in sclerotia weight, oxalate levels, or pathogenicity relative to the parental isolate.

Adaptive Cyclooctatetrathiophene-Tetrazine Macrocycles: Efficient Synthesis, Structures, and Fullerene Recognition.

A novel class of cyclooctatetrathiophene-tetrazine macrocycles was synthesized efficiently from derivatives and 3,6-dichlorotetrazine, with structures confirmed by single-crystal X-ray analysis. Macrocycles and with large cavities could be efficient for encapsulating C and C, forming a 1:1 complex with association constants exceeding 10 M. These host-guest behaviors demonstrate that -based macrocycles serve as adaptive macrocyclic hosts, generating shape complementarity cavities and optimal size to enable effective π-π interactions with the fullerenes.

Preparation and properties of sulfomethylated lignin-based highly flexible polyurethane foam.

Polyurethane (PU) can be synthesized by replacing petrochemical polyols partially with lignin, thus improving the environmental benefit and the comprehensive performance of the foam. Herein, sulfomethylated Kraft lignin (SKL) is obtained by sulfonation of Kraft lignin (KL), and then it is used as filler to synthesize the sulfomethylated lignin-based flexible polyurethane foam (SLFPUF). The effects of SKL with different sulfonations on SLFPUF are explored.

The antibody-drug conjugate SHR-A1904 for targeting CLDN18.2 in advanced gastric or gastroesophageal junction cancer: a phase 1 trial.

Claudin-18 isoform 2 (CLDN18.2), a tight junction protein expressed in non-malignant gastric epithelium and exposed on tumor cell surface during malignant transformation, is a promising therapeutic target for gastric and gastroesophageal junction (G/GEJ) cancers. SHR-A1904 is an antibody-drug conjugate comprising CLDN18.

Effects of exercise intervention on body morphology in obese college students: a systematic review and meta-analysis.

Background: Obesity, as a public health disease, is prevalent among college students and seriously affects their quality of life and academic performance. Timely intervention through an effective exercise program is of great significance in improving the physical health status of obese college students and preventing the emergence of obesity complications. Due to the lack of a systematic review of the effects of exercise intervention on the body shape of obese college students, this study aims to systematically evaluate the effects of exercise intervention on the body shape of obese college students, to provide a reference for the development of an effective exercise intervention program for the group of obese college students.

Experimental demonstration of generalized quantum fluctuation theorems in the presence of coherence.

Fluctuation theorems have elevated the second law of thermodynamics to a statistical realm by establishing a connection between time-forward and time-reversal probabilities, providing invaluable insight into nonequilibrium dynamics. While well established in classical systems, their quantum generalization, incorporating coherence and the diversity of quantum noise, remains open. We report the experimental validation of a quantum fluctuation theorem (QFT) in a photonic system, applicable to general quantum processes with nonclassical characteristics, including quasi-probabilistic descriptions of entropy production and multiple time-reversal processes.

The Boeremia exigua BeAA9 Lytic Polysaccharide Monooxygenase Is a Key Virulence Factor in Mulberry Infection.

Boeremia exigua, a phytopathogen responsible for spot diseases on leaves and stems, significantly threatens the health of a wide range of plants. Understanding its pathogenic mechanisms is essential for effective mulberry disease control. In this study, B.

Chronic low-level exposure to Pb, Hg, and Cd mixture triggers brain premature aging in rat.

Lead (Pb), mercury (Hg), and cadmium (Cd), prevalent neurotoxic heavy metals in the environment, are commonly detected at low concentrations in the blood of the general population. Our previous studies demonstrated that Pb, Hg, and Cd mixture induced neurodevelopmental toxicity even at very low levels. However, the long-term effects of low-level Pb, Hg, Cd exposure on brain aging remain unclear.

Multistage fragmentation (MS) detection enhances quantitative sensitivity and accuracy for LC-MS analysis of serum steroid hormones.

A determination method using a home-built quadrupole-linear ion trap tandem mass spectrometry (Q-LIT) multistage fragmentation function to accurate measure ultra-trace steroid hormones in human serum was established. The capability of ion trap for multistage fragmentation (MS) overcomes the limitation of triple quadrupole systems restricted to MS, enabling deeper structural characterization. Multistage fragmentation (MS3) enables the accurate detection of steroid hormones in serum samples without requiring extensive derivatization or time-consuming sample preparation and improves sensitivity by effectively reducing noise.

TRAF1 promotes the progression of Helicobacter pylori-associated gastric cancer through EGFR/STAT/OAS signalling.

Aims: Helicobacter pylori (H. pylori) is associated with various gastric diseases and is one of the pathogenic factors of gastric cancer (GC). We found that H.

Global burden trends and forecasts for MAFLD in adolescents and young adults from 1990 to 2021.

Metabolic-dysfunction associated fatty liver disease (MAFLD) is a widespread chronic liver condition that has been steadily increasing among adolescents and young adults in recent years, posing a major global public health concern. This study aims to conduct an in-depth analysis of the Global Burden of Disease (GBD) 2021 data on MAFLD, focusing on prevalence, incidence, and disability-adjusted life years (DALY) for individuals aged 15-39, spanning the period from 1990 to 2021. This research examines data from the GBD study covering 1990 to 2021 to assess the prevalence, incidence, and DALYs associated with of MAFLD in adolescents and young adults aged 15-39.

Fused exosomal targeted therapy in periprosthetic osteolysis through regulation of bone metabolic homeostasis.

The onset of periprosthetic osteolysis is mediated by wear particles following artificial arthroplasty. This manifests as a disturbed bone metabolism microenvironment, characterized by insufficient osteogenesis and angiogenesis, and enhanced osteoclastic activity. To target and remodel the homeostatic environment of bone metabolism in the sterile region around the prosthesis, we successfully pioneered the proposal and construction of a fused exosome (f-exo) system with M2 macrophage-derived exosomes (M2-exo) and urine-derived stem cell exosomes (USC-exo).

COPS5 Triggers Ferroptosis Defense by Stabilizing MK2 in Hepatocellular Carcinoma.

Sorafenib, which is proven to serve as a potent ferroptosis inducer, is used as a first-line treatment for patients with advanced hepatocellular carcinoma (HCC), but it has limited clinical benefits, mainly due to drug resistance. Herein, using genome-wide CRISPR/Cas9 knockout screening and multiple functional studies, this work identifies COP9 signalosome subunit 5 (COPS5) as a driver of sorafenib resistance and a suppressor of ferroptosis in HCC. Consistently, the amplification and overexpression of COPS5 are frequently observed in clinical HCC samples, which are associated with poor patient prognosis and might predict patient response to sorafenib therapy.

Antibiotic resistance of and related risk factors in Hunan Province: A multicenter study.

Background: Antibiotic resistance is a key factor influencing the treatment outcomes of () infection. The antibiotic resistance spectrum of varies in different regions. We investigated the current status of antibiotic resistance of in Hunan Province and analyzed the factors related to such resistance to provide strategies for the accurate clinical treatment of infection.

Exosomes Derived from Bone Marrow Mesenchymal Stem Cells Encapsulated in M2 Macrophage Cell Membrane Targeted to Inhibit Joint Periprosthetic Inflammation.

Periprosthetic osteolysis (PPOL) is a serious complication following total joint replacement surgery, and exploring treatments for this complication is of significant societal importance. Exosomes derived from bone marrow mesenchymal stem cells (BMSC-Exos, Exos) have diverse cellular functions, such as inhibiting osteoclast formation, suppressing inflammation progression, and promoting M2 macrophage polarization. However, standalone Exosomes are easily recognized and phagocytosed by the immune system, have a short half-life, and lack specificity.

Thermostability Improvement of the Chitinase from for Efficient Chitin Oligosaccharide Production via Computational Design.

The chitinase A1 from WL-12 (BcChiA1) exhibits promising potential for producing chitin oligosaccharides (CHOs), while its application is limited by its poor thermal stability. In this study, a set of thermostable variants were obtained by modifying BcChiA1 using a comprehensive strategy based on a computer-aided design. A combination of five beneficial single-point mutations (S67G/K177R/A220V/N257Y/N271E) to BcChiA1 generated a markedly improved variant, Mu5.

Efficacy of uterine artery embolization through a distal radial artery approach for treatment of uterine fibroids.

Objectives: Uterine fibroids are common benign tumors of the female reproductive system, primarily affecting women of childbearing age. These tumors worsen patients' quality of life and fertility. Current treatment options for symptomatic uterine fibroids include drug therapy, surgical intervention, and interventional procedures.

Superior Performance of the δ-MnO Ultrathin Nanoflower for Photocatalytic HCHO Oxidation.

Formaldehyde (HCHO) is carcinogenic and harmful upon prolonged exposure to human breathing. Most catalysts can eliminate HCHO at room temperature or above, but the full removal of HCHO through the catalytic process at low temperatures ( View Article and Find Full Text PDF

Triple-positive breast cancer: navigating heterogeneity and advancing multimodal therapies for improving patient outcomes.

Triple-positive breast cancer (TPBC), a unique subtype of luminal breast cancer, is characterized by concurrent positivity for estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). Owing to the crosstalk between the ER and HER2 signaling pathways, the standard of care and drug resistance of this particular subtype are difficult challenges. Recent research and clinical trials have indicated a shift in the treatment paradigm for TPBC from single-target therapies to multi-pathway, multitarget strategies aiming to comprehensively modulate intricate signaling networks, thereby overcoming resistance and enhancing therapeutic outcomes.

Removal of antibiotic resistance from wastewater in aquatic ecosystems dominated by submerged macrophytes.

Submerged macrophytes in constructed wetlands (CWs) can effectively improve wastewater quality. However, the effectiveness of different submerged macrophytes in removing antibiotic-resistant genes (ARGs) from wastewater remains unexplored. Additionally, wastewater loading in wetlands can fluctuate due to climate change, potentially affecting ARG removal efficiency.

Improved Enzymatic Properties of Chitosanase CsnMY002 from via Computational Design.

Chitooligosaccharides (COSs) are a class of functional carbohydrates with significant application prospects in food and medicine. Chitosanase CsnMY002 from the GH46 family has been used to prepare COS with controlled degrees of polymerization. To enhance the industrial applicability of CsnMY002, molecular dynamics (MD) simulations were applied to investigate the structure-property relationship.