Macrophage Nogo-B drives liver fibrosis.

Background & Aims: Liver fibrosis is characterized by sustained injury stress, chronic inflammation, and repeated cell death and repair, all of which promote the progression of end-stage liver diseases (e.g., liver cirrhosis and carcinoma).

Integrated transcriptomic and metabolomic analysis of Jinggang honey pomelo yellow spot disease to reveal the disease resistance mechanism.

Jinggangshan honey pomelo is a specialty fruit grown in Jiangxi Province, China. Pomelo yellow spot disease, also known as greasy spot disease, is a fungal pathology primarily affecting pomelo (Citrus maxima) leaves and fruits. The causative agent is the ascomycete fungus Phyllosticta citricarpa, taxonomically classified within the phylum Ascomycota.

[Expert consensus on prognostic evaluation of cochlear implantation in hereditary hearing loss].

Hearing loss is the most prevalent disabling disease. Cochlear implantation（CI） serves as the primary intervention for severe to profound hearing loss. This consensus systematically explores the value of genetic diagnosis in the pre-operative assessment and efficacy prognosis for CI.

Multifaceted Role of RIMBP2 in Promoting Hearing in Murine Cochlear Hair Cells.

The mammalian cochlea relies on outer and inner hair cells (OHCs/IHCs) for sound amplification and signal transmission. Rab3-interacting molecular binding protein 2 (RIMBP2), expressed in receptor cells and neurons at synaptic active zones, remains poorly characterized in hearing. We therefore generated a Rimbp2 knockout (KO) mouse model (Rimbp2), which exhibited severe hearing loss with elevated thresholds, prolonged latencies, and reduced amplitudes in auditory brainstem response Wave I.

CHML regulates migration and invasion in hepatocellular carcinoma via transcriptional and metabolic reprogramming.

Background: Hepatocellular carcinoma (HCC), a prevalent malignant neoplasm, presents significant therapeutic challenges. However, the key factors and mechanisms driving HCC metastasis remain incompletely understood. This study aimed to elucidate the mechanism through which CHML regulates the migration and invasion of HCC cells.

The effect of intermittent fasting on insulin resistance, lipid profile, and inflammation on metabolic syndrome: a GRADE assessed systematic review and meta-analysis.

Background: Evidence revealed that fasting intervention may have favorable effects on metabolic and inflammation profile. However, the results are controversial. This study attempted to investigate and assess the effects of fasting on cardiometabolic risk factors in adults.

Assessing cellular metabolic dynamics with NAD(P)H fluorescence polarization imaging.

Altered metabolism enables adaptive advantages for cancer, driving the need for improved methods for non-invasive long-term monitoring of cellular metabolism from organelle to population level. Here we present two-photon steady-state fluorescence polarization ratiometric microscopy (FPRM), a label-free imaging method that uses nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) autofluorescence as a functional readout of cellular metabolism. The method is simple to implement and operates an order of magnitude faster than the NAD(P)H-fluorescence lifetime imaging microscopy (FLIM) imaging modality, reducing cytotoxic stress while providing long-term monitoring capacity.

Homotypic-Targeted Mineralized Tumor Cell Probes for Ultrasensitive MRI Diagnosis of Early-Stage Malignancies.

The effectiveness of clinically used magnetic resonance imaging (MRI) contrast agents (CAs) in tumor diagnosis is limited due to their low selectivity and sensitivity. To address this, we propose inactivated cell-based contrast agents (Mn-CCs) by constructing a mineralized tumor cell. Preserving proteins and structures on the cell membrane, Mn-CCs exhibit advantages, including homotypic targeting capabilities, tumor microenvironment (TME)-activated MRI signals, and ultrahigh relaxation rates.

Role of miRNA‑214‑3p in cancer (Review).

microRNA‑214‑3p (miRNA‑214‑3p) can be mapped to the human chromosome 1q24.3 and is ~22 nucleotides in length. It has been garnering considerable attention due to its aberrant expression profile in various different types of cancer and its apparent role in regulating tumor progression.

Celebrating the fifth edition of the International Symposium on Fungal Stress - ISFUS, a decade after its 2014 debut.

The Fifth International Symposium on Fungal Stress (ISFUS) brought together in Brazil many of the leaders in the field of fungal stress responses, from fourteen countries, for four days of outstanding science ranging from basic research to studies with agricultural, medical, industrial, and environmental significance. In addition to the excellent oral and poster presentations, the Symposium organisers ensured that all participants had ample opportunity to engage, socialise, and network to exchange ideas and share research. The conference was enhanced by the world-class venue near Iguazu Falls, probably the greatest natural phenomenon in South America.

Machine learning and discriminant analysis model for predicting benign and malignant pulmonary nodules.

Background: Pulmonary Nodules (PNs) are a trend considered as the early manifestation of lung cancer. Among them, PNs that remain stable for more than two years or whose pathological results suggest not being lung cancer are considered benign PNs (BPNs), while PNs that conform to the growth pattern of tumors or whose pathological results indicate lung cancer are considered malignant PNs (MPNs). Currently, more than 90% of PNs detected by screening tests are benign, with a false positive rate of up to 96.

Suppression of cofilin-1 promotes invasion in 3D hyaluronic acid matrices by promoting actin-based protrusions.

Contributions of the actin turnover machinery to cell motility have been extensively studied in traditional two-dimensional (2D) culture paradigms. However, much remains unknown about how these proteins contribute to three-dimensional (3D) motility, particularly in matrices lacking strong contact guidance cues. Here, we explore this question in the context of glioblastoma (GBM) cell invasion through 3D hyaluronic acid (HA) hydrogels.

Impacts of Behavioral Compliance on Weight-Loss and Metabolic Profile in a Smartphone App-Based Lifestyle Intervention or Plus Dietitian Supporting: A Randomized and Controlled Trial Among Chinese.

Unlabelled: Mobile health technologies provided innovative solutions for lifestyle interventions and offered reliable methods to evaluate behavioral phenotypes during such interventions. To systematically quantify the impacts of behavioral compliance on weight-loss and metabolic profiles during lifestyle intervention, a total of 395 Chinese adults with overweight/obesity (BMI ≥ 24 kg/m) or central obesity (waistline ≥ 90 cm for men or ≥ 80 cm for women) were randomly assigned to a smartphone app-based arm (SAA, n = 197) or smartphone app plus dietitian arm (SADA, n = 198) for 6 months. Compliance scores (0-5) were determined based on fulfilling five behavioral tasks: completing online courses, wearing a smart band, and recording weight, food intake, and blood pressure.

AAV gene therapy for autosomal recessive deafness 9: a single-arm trial.

Gene therapy for congenital deafness has shown promising results in children but lacks data in older populations. We conducted a single-arm trial of adeno-associated virus (AAV)-OTOF gene therapy using the Anc80L65 capsid in ten participants with autosomal recessive deafness 9 aged 1.5 to 23.

Intracytoplasmic Sperm Injection (ICSI) Timing Affects the Influence of Sperm DNA Fragmentation on ICSI Outcomes.

Oocytes have repair ability for paternally derived DNA damage which depends on oocyte quality. Prolonged post ovum pick-up (OPU) incubation may lead to oocyte aging, which is linked to changes in oocyte ultrastructure and function. In order to study whether the effect of sperm DNA fragmentation (SDF) on intracytoplasmic sperm injection (ICSI) outcomes influenced by ICSI timing, we conducted this retrospective study included 1,331 ICSI cycles from March 2016 to December 2022.

Colistin enhances caspofungin antifungal efficacy against Aspergillus fumigatus by modulating calcium homeostasis and stress responses.

Fungal infections cause more than 2.5 million deaths a year. Due to emerging antifungal drug resistance, novel strategies are urgently needed to combat life-threatening fungal diseases.

UBXD8 promotes lung cancer progression and activates the HIF-1α pathway.

UBXD8, a ubiquitin regulatory X domain-containing protein, is highly expressed in advanced tumor stages and is associated with reduced survival rates. In this study, we found that knockdown of UBXD8 resulted in a significant decline in non-small cell lung cancer (NSCLC) cell proliferation and migratory capacity in vitro, as well as a reduction in tumor growth within xenograft models, suggesting its potential as a prognostic marker. Moreover, we identify UBXD8 as a positive modulator of hypoxia inducible factor (HIF) signaling.

Single-cell profiling identifies hair cell SLC35F1 deficiency as a signature of primate cochlear aging.

Cochlear aging causes substantial hearing impairment in older adults, yet primate-specific mechanisms remain poorly characterized. Our comprehensive analysis combining single-cell and histopathological profiling in aging Macaca fascicularis demonstrates progressive cochlear degeneration featuring accelerated sensory hair cell loss, senescent spiral ganglion neurons with elevated neuroinflammation, and marked stria vascularis atrophy. We discovered that downregulation of transmembrane transport proteins, particularly SLC35F1, serves as a critical biomarker of hair cell aging.

The E3 ubiquitin ligase SMURF2 protects against atherosclerosis by inhibiting endothelial inflammation.

Endothelial dysfunction is a key driver of sustained and chronic vascular inflammation, which plays a critical role in the progression of atherosclerotic disease. Despite its significance, the molecular mechanisms underlying vascular endothelial inflammation remain poorly understood. Ubiquitination, a widespread post-translational modification, regulates a wide range of biological processes and is essential for maintaining cellular homeostasis in both physiological and pathological conditions.

Dual energy CT-based Radiomics for identification of myocardial focal scar and artificial beam-hardening.

Background: Computed tomography is an inadequate method for detecting myocardial focal scar (MFS) due to its moderate density resolution, which is insufficient for distinguishing MFS from artificial beam-hardening (BH). Virtual monochromatic images (VMIs) of dual-energy coronary computed tomography angiography (DECCTA) provide a variety of diagnostic information with significant potential for detecting myocardial lesions. The aim of this study was to assess whether radiomics analysis in VMIs of DECCTA can help distinguish MFS from BH.

Efficacy and safety of Xiao'er Fengre Qing oral liquid Oseltamivir in treating pediatric influenza (wind-heat invading the defense syndrome): a multicenter, randomized, non-inferiority trial.

Background: Xiao'er Fengre Qing Oral Liquid (XFQOL) is developed based on the classical traditional Chinese medicinal formula . Compared to the original formulation, XFQOL exhibits enhanced heat-clearing, detoxification, and fever reduction, which can effectively address the common complications associated with influenza in children and is well-suited for pediatric use. However, there is currently a lack of high-quality evidence from clinical trials to support its efficacy and safety in clinical applications.

Hyper-gap transparent conductor.

An elusive conductor with perfect optical transparency holds revolutionary potential for fields such as optoelectronics and nanophotonics. Such a hypothetical metal would possess a spectral gap-a 'hyper-gap'-in its absorption spectrum, separating the intraband and interband absorptions, in which optical losses could vanish. Currently, this property is achievable only within the bandgap of insulators.

Anxiety is a risk prognosis factor for hepatocellular carcinoma with portal vein tumor thrombus who underwent hepatic arterial infusion chemotherapy: a propensity score-matching cohort study.

Background: Increasing evidence indicates that psychological factors play a role in tumor progression. This study aims to explore the impact of anxiety disorder on the prognosis of hepatocellular carcinoma (HCC) patients with portal vein tumor thrombus (PVTT) who underwent hepatic arterial infusion chemotherapy (HAIC).

Methods: A propensity score-matching cohort study was conducted in 68 HCC patients with PVTT who underwent HAIC between January 2020 and December 2023.