Self-Enhanced Ratiometric ECL Strategy Based on a Dual-Emission Ce/Tb-MOF Luminophore with Spatially Resolved Coreactant Delivery for Ultrasensitive Pro-GRP Detection.

Dual-signal-output ratiometric electrochemiluminescence (ECL) platforms offer a promising solution to address the instability and inaccuracies associated with single-signal ECL detection. However, most existing ratiometric systems still rely on two independent luminophores, inevitably increasing the system complexity and compromising the detection accuracy. In this study, a bimetallic dual-ligand ECL luminophore, Ce/Tb-MOF, was synthesized via a solvothermal approach, exhibiting distinct anodic and cathodic ECL emissions from a single luminophore.

Adopting Early Essential Newborn Care (EENC) in the Delivery Room: An Implementation Study From China.

Background: Early Essential Newborn Care (EENC) is a highly beneficial and cost-effective set of evidence-based interventions for newborns and their mothers. However, the implementation of EENC as part of routine clinical practice in the delivery room has not yet been achieved in China. The purpose of this study was to describe the adoption of EENC in general hospitals in China and to evaluate its implementation impacts.

A Token Stimulus Receptor Tuned to Gluconapin in the Cabbage White Butterfly.

butterflies recognize host Brassicaceae plants via sensing glucosinolates, but the molecular mechanism remains unclear. Here, we cloned five gustatory receptor genes highly expressed in larval mouthparts, adult foreleg tarsi, and the proboscis of and then ectopically expressed each of them in neurons of and determined responses of the neurons to glucosinolates. Among them, only > neurons respond to gluconapin dose-dependently.

Establishment of region-, age- and sex-specific reference intervals for aldosterone and renin with sandwich chemiluminescence immunoassays.

Objectives: Accurate measurement of plasma aldosterone (ALD) and renin is important in primary aldosteronism screening and diagnosis. The high variability of ALD and renin due to salt intake, age, and sex, as well as the lack of immunoassay standardization necessitates the establishment of region-, age-, sex- and assay-specific reference intervals (RIs). This study established RIs for ALD and renin using novel sandwich chemiluminescent immunoassays.

Machine learning-driven prognostic prediction model for composite small cell lung cancer: identifying risk factors with network tools and validation using SEER data and external cohorts.

Background: Lung cancer continues to be the primary cause of cancer-related mortality globally, with combined small cell lung carcinoma (C-SCLC) constituting a relatively uncommon yet highly aggressive subset of this disease. Despite its clinical significance, limited efforts have been made to develop survival prediction models tailored to the clinical characteristics of C-SCLC patients. Additionally, the interpretability of existing models remains limited.

The pronounced cytotoxic effects of chimeric antigen receptor T cells targeting B7-H3 in organoids and liver xenografts derived from colorectal cancer patients.

Background: The application of chimeric antigen receptor (CAR)-T cells in solid tumors is hindered due to the lack of specific tumor antigen and limited clinical efficacy. Our aim is to develop and validate novel CAR-T cell therapy against metastatic colorectal cancer (CRC).

Methods: By analyzing the expression of B7-H3 in CRC tissue and cell lines using immunohistochemistry (IHC) and flow cytometry, respectively, we identified B7-H3 as a potential target in CRC.

Development and validation of a LC-MS/MS method for the simultaneous determination of simnotrelvir and ritonavir in human serum and bronchoalveolar lavage fluid.

"Xiannuoxin" (simnotrelvir/ritonavir) is a novel anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) drug developed in China, which plays an antiviral role by inhibiting 3C-like protease (3CL). At present, it has been put into clinical use, while a simple, accurate and sensitive detection method is urgently needed for the quantification of simnotrelvir/ritonavir in human serum and bronchoalveolar lavage fluid (BALF) to ensure safe and efficacious antiviral therapeutics. In this study, we developed a liquid chromatography tandem mass spectrophotometry (LC-MS/MS) method for the simultaneous determination of simnotrelvir, ritonavir and urea concentrations in human serum and BALF samples.

Ultrasensitive photoelectrochemical immunosensor based on floral cluster SnS/ZnCdS heterostructure for the detection of CA199.

The early and accurate detection of tumor markers is crucial for cancer diagnosis, prognosis, and treatment monitoring. Carbohydrate antigen 199 (CA199), as a key biomarker of pancreatic, gastric, and colorectal cancers, is widely used in the clinical management. The development of sensitive, rapid and cost-effective detection methods for CA199 is of paramount importance in improving early detection rates and patient outcomes.

[Intraspecific variation of Forsythia suspensa chloroplast genome].

Forsythia suspensa is a traditional Chinese medicine and a commonly used landscaping plant. Its dried fruit is used in medicine for its functions of clearing heat, removing toxins, reducing swelling, dissipating masses, and dispersing wind and heat. It possesses extremely high medicinal and economic value.

Organic Ligand-Enhanced Covalent Fe Coordination in NiFe LDH for Efficient Biomass Upgrading.

Electrocatalytic conversion of biomass-derived 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) presents a promising route for the bio-renewable polymer industry. However, the development of efficient catalysts remains a key challenge. Herein, a NiFe layered double hydroxide (LDH) catalyst modified with an organic carboxylate ligand, trimellitic acid (TA) (denoted as NiFe LDH-TA) is designed.

Mechanistic insights into resistance mechanisms to T cell engagers.

T cell engagers (TCEs) represent a groundbreaking advancement in the treatment of B and plasma cell malignancies and are emerging as a promising therapeutic approach for the treatment of solid tumors. These molecules harness T cells to bind to and eliminate cancer cells, effectively bypassing the need for antigen-specific T cell recognition. Despite their established clinical efficacy, a subset of patients is either refractory to TCE treatment (e.

A large-scale multicenter study of reference intervals and clinical potential for homocysteine-folate cycle metabolites in Northern Chinese population.

Objectives: The study was conducted to establish the reference intervals of homocysteine-folate cycle metabolites based on the healthy population from multiple centers in northern China, and determine their clinical significance in the diagnosis of related diseases.

Methods: 1222 healthy individuals were recruited from four hospitals in northern China. The levels of serum HCY-related metabolites were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Deorphanization of Pheromone Receptors and Discovery of a Novel Agonist for Sex Pheromone Communication in Diamondback Moths.

Sex pheromone communication is an essential component of mate recognition in moths. In this study, we heterologously expressed male-biased pheromone receptors (PRs) of diamondback moths, in OR67d neurons and determined their responses toward sex pheromonal compounds. The neurons expressing PxylOR59, PxylOR13, and PxylOR46 specifically responded to three sex pheromone components, Z11-16:Ald, Z11-16:Ac, and Z11-16:OH, respectively.

Synergizing Anti-Cancer Drug Combinations With Dual-View Hypergraph Representation Fusion.

Drug combination therapy plays a vital role in disease treatment, including cancer, as it contributes to treatment efficacy and can alleviate the effect of drug resistance. Although clinical trials and screening may provide valuable information about synergistic drug combinations, they suffer from challenging combinatorial space. Multiple methods are proposed to address those issues.

Research on application of diamond FAT for black lithium tantalate wafer processing based on nanoindentation and scratch techniques.

Lithium tantalate (LiTaO, LT) single crystal has been widely applied in the fields of electro-optical and piezoelectric devices. In this study, diamond fixed-abrasive tools (FAT) for LT were fabricated using consolidation abrasive processing technology to streamline the LT wafers processing and enhance overall effectiveness. The material properties and the critical depth for the ductile-to-brittle transition of LT were examined through nanoindentation and scratch techniques.

Ecological risk assessment and response prediction caused by LUCC in the central Yunnan Province.

With the acceleration of urbanization, unreasonable land use poses a serious threat to ecological security. However, there is still some space for improvement in the existing assessment of ecological risks (ERs) caused by land use/land cover change (LUCC). Therefore, this study takes the central Yunnan Province (CYP) as an example, and uses the Patch-Generating Land Use Simulation (PLUS) model and the Integrated Valuation of Ecosystem Services and Trade-offs (InVEST) model to simulate the land use/land cover (LULC) in different scenarios in the future, calculate the ecosystem services (ESs) from 2000 to 2020 and the next 20 years, as well as ERs of various types of ESs caused by LUCC.

Time-resolved spectroscopy uncovers deprotonation-induced reconstruction in oxygen-evolution NiFe-based (oxy)hydroxides.

Transition-metal layered double hydroxides are widely utilized as electrocatalysts for the oxygen evolution reaction (OER), undergoing dynamic transformation into active oxyhydroxides during electrochemical operation. Nonetheless, our understanding of the non-equilibrium structural changes that occur during this process remains limited. In this study, utilizing in situ energy-dispersive X-ray absorption spectroscopy and machine learning analysis, we reveal the occurrence of deprotonation and elucidate the role of incorporated iron in facilitating the transition from nickel-iron layered double hydroxide (NiFe LDH) into its active oxyhydroxide.

Non-Invasive Diagnosis of Moyamoya Disease Using Serum Metabolic Fingerprints and Machine Learning.

Moyamoya disease (MMD) is a progressive cerebrovascular disorder that increases the risk of intracranial ischemia and hemorrhage. Timely diagnosis and intervention can significantly reduce the risk of new-onset stroke in patients with MMD. However, the current diagnostic methods are invasive and expensive, and non-invasive diagnosis using biomarkers of MMD is rarely reported.

Fault diagnosis method of rolling bearing based on SSA-VMD and RCMDE.

To address the limitations of weak information extraction of rolling bearing fault features and the poor generalization performance of diagnostic methods, a novel method was proposed based on sparrow search algorithm (SSA)-Variational Mode Decomposition (VMD) and refined composite multi-scale dispersion entropy (RCMDE). Firstly, SSA optimized the key parameters of VMD to decompose the fault signal. The time-frequency domain comprehensive evaluation factor algorithm was then employed to select the sensitive intrinsic mode function (IMF) components for reconstruction.

Decoding aging clocks: New insights from metabolomics.

Chronological age is a crucial risk factor for diseases and disabilities among older adults. However, individuals of the same chronological age often exhibit divergent biological aging states, resulting in distinct individual risk profiles. Chronological age estimators based on omics data and machine learning techniques, known as aging clocks, provide a valuable framework for interpreting molecular-level biological aging.

Metagenomics and metaproteomics reveal the effects of sludge types and inoculation modes on N,N-dimethylformamide degradation pathways and the microbial community involved.

This study demonstrated the effects of the sludge type and inoculation method on the N,N-dimethylformamide degradation pathway and associated microbial communities. The sludge type is critical for DMF metabolism, with acclimatized aerobic sludge having a significant advantage in terms of DMF metabolism performance, whereas acclimatized anaerobic sludge has a reduced DMF metabolism capacity. Metagenomic revealed increased abundances of Methanosarcina, Pelomona and Xanthobacter in the adapted anaerobic sludge, suggesting that anaerobic sludge can utilize the methyl products produced by DMF metabolism for growth.

The dual-functional role of carboxylate in a nickel-iron catalyst towards efficient oxygen evolution.

The efficiency of the oxygen evolution reaction (OER) is severely limited by the sluggish proton-coupled electron transfer processes and inadequate long-term stability. Herein, we introduce a carboxylate group (TPA) to modify NiFe layered double hydroxide (NiFe LDH@TPA), resulting in notable improvements in both activity and stability. A combination of spectroscopic and theoretical investigations reveals the dual-functional role of incorporated TPA.

A photoelectrochemical sensor based on InS/AgInS in situ Z-type heterojunction with "photo-modulated interface charge" for sensitive detection of Programmed Death-Ligand 1.

The construction of heterostructure photoelectrodes can enhance the performance of photoelectrochemical (PEC) sensors. However, it is still a critical challenge to achieve efficient transfer of interface carriers. In this paper, we propose a strategy of "photo-modulated interface charge" to design a PEC sensor based on a hollow hexagonal tubular InS/AgInS in situ Z-type heterojunction for the susceptible detection of Programmed Death-ligand 1 (PD-L1).

Clinical potential of serum prostaglandin A2 as a novel diagnostic biomarker for hepatocellular cancer.

Background: Hepatocellular cancer (HCC) is one of the most harmful tumors to human health. Currently, there is still a lack of highly sensitive and specific HCC biomarkers in clinical practice. In this study, we aimed to explore the diagnostic performance of prostaglandin A2 (PGA2) for the early detection of HCC.