Detection of phosphatidylserine exposure on Mycoplasma hyorhinis by flow cytometry.

Mycoplasma often contaminates eukaryotic cell cultures. Determining Mycoplasma cell viability is important when evaluating the cellular response to drug treatment. One method to test cell viability is using a membrane-impermeant nucleic acid dye that is generally excluded from viable cells.

Sintilimab-induced intestinal obstruction and hemorrhage of the digestive tract: a case report.

Immune checkpoint inhibitors (ICIs) have become an important part of malignant tumor therapy. However, some adverse reactions follow ICIs therapy. The incidence of immune-mediated colitis (IMC) has also increased.

PPARG-centric transcriptional re-wiring during differentiation of human trophoblast stem cells into extravillous trophoblasts.

Peroxisome proliferator-activated receptor gamma (PPARG) is a nuclear receptor family transcription factor (TF) critical for adipogenesis, lipid metabolism, insulin sensitivity, and inflammation. It has also been known to play essential roles in trophoblast development and placentation. Dysregulation of PPARG in trophoblast differentiation has been implicated in pregnancy complications, such as pre-eclampsia and gestational diabetes.

AB-side growth strategy: a new approach to regulate the electrocatalytic performance of metal-organic framework composite materials.

In this work, we propose a new AB-side growth strategy for the first time to enhance the electrocatalytic activity and stability of pristine metal-organic framework (MOF) materials. This work provides a new perspective and synthetic strategy to construct high-performance and highly stable MOF composite materials.

Ultrasensitive photoelectrochemical immunosensor based on floral cluster SnS/ZnCdS heterostructure for the detection of CA199.

The early and accurate detection of tumor markers is crucial for cancer diagnosis, prognosis, and treatment monitoring. Carbohydrate antigen 199 (CA199), as a key biomarker of pancreatic, gastric, and colorectal cancers, is widely used in the clinical management. The development of sensitive, rapid and cost-effective detection methods for CA199 is of paramount importance in improving early detection rates and patient outcomes.

Pyrrolidinium Fullerenes as YTHDF1 Inhibitors for Targeted Tumor Therapy.

Cancer remains a leading cause of global morbidity and mortality, necessitating the development of novel targeted therapies. This study explores the therapeutic potential of pyrrolidinium fullerenes as YTH N-methyladenosine RNA binding protein 1 (YTHDF1) inhibitors for cancer treatment. A series of functionalized pyrrolidinium fullerenes is synthesized and characterized, including C-(N,N-dimethyl-pyrrolidinium iodide) (NDMPFI), C-(N-methyl-N-benzyl-pyrrolidinium iodide) (NMBPFI), and C-(N-methyl-N-hydroxyethyl-pyrrolidinium iodide) (NMHPFI).

Fabricated CoFePBA/NF-Derived Mixed Metal Sulfide as Multifunctional Catalysts for Enhanced Urea-Water Electrolysis.

This study demonstrates the successful fabrication of mixed-metal CoFePBA/NF with hierarchical 1D + 3D string-bead morphology through in situ conversion, followed by sulfidation to obtain highly active s-CoFePBA/NF. The optimized s-CoFePBA/NF (500 °C) demonstrates exceptional trifunctional electrocatalytic performance, achieving ultralow overpotentials of 226 mV (OER) and 127 mV (HER) at 10 mA cm, along with a remarkable UOR potential of 1.317 V.

Protocol for purification, crystallization, and structure determination of the mpox virus mRNA cap N7 methyltransferase complex.

The mpox virus (MPXV) mRNA cap N7 methyltransferase (RNMT) methylates guanosine at mRNA 5'-cap N7 positions to enable immune evasion. Here, we present a protocol for E1-E12 complex purification and crystallization. We describe steps for rational sequence design of the complex, co-expression in E.

RING1 dictates GSDMD-mediated inflammatory response and host susceptibility to pathogen infection.

RING1 is an E3 ligase component of the polycomb repressive complex 1 (PRC1) with known roles in chromatin regulation and cellular processes such as apoptosis and autophagy. However, its involvement in inflammation and pyroptosis remains elusive. Here, we demonstrate that human RING1, not RING2, promotes K48-linked ubiquitination of Gasdermin D (GSDMD) and acts as a negative regulator of pyroptosis and bacterial infection.

Establishment, identification, and transcriptome analysis of a Sertoli cell line from ovoviviparous black rockfish Sebastes schlegelii.

Black rockfish (Sebastes schlegelii) is an economically important species with a unique ovoviviparous reproductive mode, in which reproduction is limited by incomplete fertilization. In order to understand the mechanism of spermatogenesis of black rockfish, a cell line derived from the testis, named SSTC, was successfully established and cultured in L-15 medium at 25 °C, and it was passaged to the 50th generation. The SSTC exhibited fibroblast-like and epithelial-like morphology during in vitro culture, and 62% of the SSTC retained the diploid karyotype with 48 chromosomes by the 30th passage (P30).

Identification and characteristic analysis of PavUGT48 as a novel UDP-glycosyltransferase with dual functions on anthocyanin and amygdalin biosynthesis in sweet cherry.

UDP-glycosyltransferase (UGT) is essential for fruit development, but its specific role in sweet cherry fruits has not been clearly defined. This study identified a new differentially expressed UGT gene, FUN_010048, by analyzing transcriptomic data from various colored regions of bicolored fruits. The 187 UGT family members were identified in the sweet cherry genome of 'Tieton'.

Decoy receptor 3 as a prognostic biomarker for sepsis and septic shock according to the Sepsis-3 definitions.

Objectives: The present study was conducted to reappraise the prognostic value of decoy receptor 3 (DcR3) for patients with sepsis and septic shock according to the latest Sepsis-3 definitions.

Methods: Subjects suffering from sepsis or septic shock were enrolled within 6 h of admission. The Sequential Organ Failure Assessment (SOFA) score and the plasma levels of DcR3, C-reactive protein, procalcitonin, and interleukin-6 were measured.

Metabolic responses to starvation in the soft-shelled turtle (Pelodiscus sinensis) revealed by integrated metabolome and transcriptome analysis.

Animals frequently suffer from starvation throughout their life cycle; however, the mobilization and utilization of energy sources can differ. To clarify the fundamental mechanisms underlying energy mobilization and metabolic adjustment in response to food deprivation in the soft-shelled turtle (Pelodiscus sinensis), eighty turtles (initial body weight, 51.81 ± 0.

Exploring P-(Fe,V)-Codoped Metastable-Phase β-NiMoO for Improving the Performance of Overall Water Splitting.

It is especially essential to develop high-performance and low-cost nonprecious metal catalysts for large-scale hydrogen production. A large number of electrochemical catalysts composited by transition metal centers has been reported; however, it is still a great challenge to design and manipulate target electrocatalysts to realize high overall water-splitting activity at the atomic level. Herein, we develop totally new P-(Fe,V)-codoped metastable-phase β-NiMoO.

FGFR3 Upregulates Interferon-Stimulated Genes Via the JAK1-STAT1 Signaling Pathway in HPV2 E2 Stable Expressing Keratinocytes.

Human papillomavirus (HPV) infections are prevalent skin infectious diseases. While there are no specific anti-HPV drugs available, understanding the viral mechanisms could lead to novel therapeutic strategies. Verruca vulgaris, a common HPV infection, is frequently encountered in dermatological clinics.

Effect of tacrolimus with mycophenolate mofetil or cyclophosphamide on the renal response in systemic lupus erythematosus patients.

Objective: This study aimed to determine the therapeutic efficacy of tacrolimus (TAC) with mycophenolate mofetil (MMF) or cyclophosphamide (CYC) on the renal response in systemic lupus erythematosus (SLE) patients.

Methods: A retrospective cohort study based on medical data was conducted among SLE patients who took at least one of the following medicines in 2010-2021: TAC, MMF and CYC. The odds ratio (OR) and 95% confidence interval (CI) were calculated, and the synergistic interaction was estimated using logistic regression models.

The causal relationship between diabetes mellitus and the risk of sensorineural hearing loss: A Mendelian randomization study.

An increasing body of evidence suggests that diabetes mellitus (DM) plays a role in sensorineural hearing loss (SNHL). However, the specific causal relationship between DM and SNHL remains partially uncertain. This study aimed to investigate the causal relationship between DM and the risk of SNHL using a Mendelian randomization (MR) study.

Effects of different rootstocks on fruit quality and non-volatile flavor-related compounds of sweet cherry 'summit'.

In practical sweet cherry production, grafting onto rootstocks is a common practice to enhance environmental adaptability. Rootstocks play a crucial role in influencing scion growth and fruit quality by regulating the absorption and utilization of mineral elements. In this study, the influence of five rootstocks with or without root fertilization during the fruit color conversion period on the fruit quality of sweet cherry 'Summit' was observed.

Anionic Hofmeister Effect Regulated Conductivity in Polyelectrolyte Hydrogels for High-Performance Supercapacitor.

The Hofmeister effect not only affects the stability and solubility of protein colloids but also has specific effects on the polymer molecules. Here, the impact of the Hofmeister effect on the electrochemical properties of polyelectrolyte hydrogels at room temperature and subzero temperature studied for the first time. Polyelectrolyte hydrogels exhibit an anti-polyelectrolyte effect in low concentrations of ammonium salt, while they exhibit an obvious Hofmeister effect in high concentrations of ammonium salt.

[Analysis of Clinical Characteristics and Laboratory Indicators in Patients with Solid Malignant Tumor-Associated Venous Thromboembolism].

Objective: To analyze the clinical features and laboratory indicators in patients with solid malignant tumor-associated venous thromboembolism (Ta-VTE), and to study the risk factors for Ta-VTE.

Methods: The hospitalized patients with VTE in Guizhou Provincial People's Hospital from January to December 2020 were enrolled, and they were divided into Ta-VTE group and pure VTE group based on the presence or absence of solid malignant tumor. The differences in clinical data and laboratory indicators between the two groups were analyzed, and the indicators with significant differences were included in logistic regression model to analyze the risk factors of Ta-VTE.

Systematic mapping of TF-mediated cell fate changes by a pooled induction coupled with scRNA-seq and multi-omics approaches.

Transcriptional regulation controls cellular functions through interactions between transcription factors (TFs) and their chromosomal targets. However, understanding the fate conversion potential of multiple TFs in an inducible manner remains limited. Here, we introduce iTF-seq as a method for identifying individual TFs that can alter cell fate toward specific lineages at a single-cell level.

In Situ Exfoliation Growth Strategy Realizing Controlled Synthesis of 3D to 2D MOF Materials as High-Performance Electrochemical Biosensors.

Two-dimensional (2D) metal-organic framework (MOF) nanosheets with large surface area, ultrathin thickness, and highly accessible active sites have attracted great research attention. Developing efficient approaches to realize the controllable synthesis of well-defined 2D MOFs with a specific composition and morphology is critical. However, it is still a significant challenge to construct thin and uniform 2D MOF nanosheets and resolve the reagglomeration as well as poor stability of target 2D MOF products.

The transcriptional regulatory network modulating human trophoblast stem cells to extravillous trophoblast differentiation.

During human pregnancy, extravillous trophoblasts play crucial roles in placental invasion into the maternal decidua and spiral artery remodeling. However, regulatory factors and their action mechanisms modulating human extravillous trophoblast specification have been unknown. By analyzing dynamic changes in transcriptome and enhancer profile during human trophoblast stem cell to extravillous trophoblast differentiation, we define stage-specific regulators, including an early-stage transcription factor, TFAP2C, and multiple late-stage transcription factors.