Comparison of 10-year follow-up result of hybrid surgery and anterior cervical discectomy and fusion for the treatment of contiguous three-level cervical degenerative disc diseases: risk factors for heterotopic ossification.

Purpose: To compare the long-term clinical outcomes and radiographic findings between hybrid surgery (HS) and anterior cervical discectomy and fusion (ACDF) for three-level cervical degenerative disc disease (CDDD). And the incidence, prognosis, and potential risk factors of heterotopic ossification (HO) more than 10 years after Bryan prosthesis replacement in HS has been explored.

Methods: From January 2007 to December 2014, a total of 46 patients who underwent either HS (n = 26) or ACDF (n = 20) for consecutive three-level CDDD were retrospectively analyzed.

[Study on the effect of postoperative implant fusion after anterior cervical discectomy and fusion by applying nano-hydroxyapatite/collagen composite in patients with low bone mass cervical spondylosis].

Objective: To explore the effect of nano-hydroxyapatite/collagen composite (nHAC) on bone graft fusion after anterior cervical discectomy and fusion (ACDF) in patients with cervical spondylosis and low bone mass.

Methods: A retrospective analysis was conducted on 47 patients with low bone mass who underwent ACDF from 2017 to 2021. They were divided into the nHAC group and the allogeneic bone group according to different bone graft materials.

Identification and expression analysis of sex-related genes in the pine wood nematode insect vector Monochamus saltuarius (Coleoptera: Cerambycidae).

Sex determination in insects is a product of long-term evolutionary selection, characterized by intricate and highly variable mechanisms across different taxa. The Sakhalin pine sawyer, Monochamus saltuarius, has recently been recognized as a vector for the pine wood nematode in China. However, the candidate genes and molecular mechanisms underlying sex determination in M.

CmPLL16a and CmPLL16b Regulate Cell Wall Remodelling and Somatic Embryogenesis in Castanea mollissima.

Chinese chestnut (Castanea mollissima [C. mollissima] Blume), an important nut crop with recalcitrant seeds and contributor to forest ecosystem services, faces challenges due to seed abortion, which seriously influences yield. In this study, stark microstructural and ultrastructural differences were observed between fertile and abortive seeds, with the abortive seeds exhibiting abnormal cell wall shapes and irregular thickening.

Tough, Antibacterial, and Antithrombogenic Hydrogel Coatings for Blood-Contacting Silicone Medical Devices.

Silicone medical devices are widely used as various medical devices due to their excellent advantages when compared with petroleum-derived polymers. Thrombosis and bacterial infections are two vital challenges of blood-contacting silicone devices. The traditional strategies used for contact antibacterial silicone typically facilitate thrombosis due to electrostatic interactions.

HPLC fingerprinting-guided determination of the optimal harvest period for Jinsi Huangju (Chrysanthemum morifolium Ramat.) and to elucidate its anti-inflammatory mechanisms.

'Jinsi Huangju' (JSHJ, Chrysanthemum morifolium Ramat.) is a medicinal plant traditionally used to manage inflammation. However, its optimal harvesting period and underlying anti-inflammatory mechanisms remain insufficiently explored.

The Ste20 Homologue FvM4K1 Regulates Organ Size via Hippo Signalling Pathway in Woodland Strawberry (Fragaria vesca).

Strawberry fruit size is critical for its marketability. However, organ size control is a complex process regulated by various signalling pathways. In animals, the Hippo signalling pathway acts as a negative regulator of organ size, with Ste20 kinase being a key component.

Three-Dimensional-Printed Meat Products with Lycopene-Functionalized Yeast Pickering Emulsions as Fat Replacer.

Due to the health-driven demand for fat replacers in meat products, Lycopene (Lyc)-loaded yeast protein (YP) high internal phase Pickering emulsions (HIPPEs) were explored as fat replacers for 3D-printed meat products. HIPPEs with varying Lyc concentrations were formulated, and their encapsulation efficiency and antioxidant activity (DPPH and ABTS assays) were evaluated. The encapsulation efficiency of Lyc exceeded 90% for all samples.

Gut microbiome and metabolome interactions in Crohn's disease: mechanistic insights into exclusive enteral nutrition-induced remission.

Background: Exclusive enteral nutrition (EEN) is a first-line therapeutic approach for inducing remission in Crohn's disease. However, the underlying mechanisms of its action remain poorly understood. This study aims to investigate the effects of EEN on the gut microbiome and metabolome of CD patients and to elucidate the mechanisms responsible for EEN-induced remission.

Bioconditioning-driven enhancement of sewage sludge dewaterability via EPS modification by .

This study proposes a bioconditioning method using () to improve sewage sludge dewaterability. Application of whole culture reduced the specific resistance to filtration (SRF) by 87.9%.

[Current status of acupuncture education and reflections on future reforms].

Education is a crucial element in the development of acupuncture as a discipline, providing essential talent support for its future advancement. A structured interview was conducted with renowned acupuncture expert Professor , focusing on key topics such as the core of acupuncture education, the connotation and development of acupuncture textbooks, and acupuncture teaching models. Through in-depth discussion, the current problems in acupuncture education were analyzed, and possible solutions were explored, aiming to offer ideas for the innovative development of acupuncture education.

Analysis of Genetic Diversity and Core Germplasm Construction of Siebold and Zucc. Using Simple Sequence Repeat Markers and Morphological Traits.

This study investigates the taxonomic status, phylogenetic relationships, and genetic diversity of Japanese chestnut ( Siebold & Zucc.) in Liaodong, China, and across East Asia. Additionally, it evaluates core germplasm resources through cluster and population structure analyses using simple sequence repeat (SSR) marker data from 13 , , and 27 , and 142 Japanese chestnut resources.

The role of oxidative stress in spinal cord ischemia reperfusion injury: mechanisms and therapeutic implications.

Spinal cord ischemia/reperfusion injury (SCIRI) is a serious disease that leads to the loss of sensory and motor functions and is a common complication after spinal cord injury, spinal cord degeneration or thoracic and abdominal aortic surgery. At present, the spinal cord is mainly protected from ischemic injury through treatment strategies such as hypothermia, surgery and drug assistance, but these intervention measures cannot effectively improve these conditions. SCIRI is a complex process that leads to cell damage and death.

Overexpression of pectate lyase gene FvPLB leads to pollen abortion in Fragaria vesca.

The pectate lyase gene FvPLB has been implicated in the regulation of fruit softening in previous studies. We have now also found that abortions occurred in FvPLB overexpression lines. After overexpression of FvPLB, the cellulose content in the pollen grain and pollen tube wall was not affected, but the esterified and de-esterified pectin content in the pollen grain and pollen tube wall was significantly reduced, resulting in looser cell walls and deeper pollen grain deformation.

Integrated Analyses to Identify the Roles of in Frailty and Hypertension.

Background: With aging, frailty and hypertension become increasingly prevalent comorbidities in the older population. Therefore, the aim of the study is to identify effective druggable targets for these conditions.

Methods: We performed a two-sample Mendelian randomization analysis to assess the causal effects of 2532 druggable genes on frailty, hypertension, systolic blood pressure and diastolic blood pressure.

Construction of a novel online calculator for prediction of osteoporosis risk in Chinese type 2 diabetes patients.

Background: Type 2 diabetes (T2D) has been established as an independent risk factor for osteoporosis, often resulting in a poor prognosis. Thus, it is crucial for clinicians to diagnose osteoporosis in diabetic patients. This study aimed to develop a prediction model for osteoporosis in people with T2D from China.

Role of CORO1A in Regulating Immune Homeostasis of Mammary Glands and Its Contribution to Clinical Mastitis Development in Dairy Cows.

Immune homeostasis refers to the immune system's ability to maintain a dynamic balance, defend against infections while preventing excessive inflammation, and preserve normal physiological activity. However, its regulatory role in the mammary glands (MGs) of cows with clinical mastitis (CM) remains unclear. This study examined MG tissue samples collected from healthy Holstein cows and those with CM caused by ( = three per group) to identify candidate biomolecular targets involved in immune homeostasis in dairy cows affected by mastitis through a proteomics-based bioinformatic analysis and analyze their expression and localization in MG tissues.

Preventive and therapeutic effects of Tanshinone IIA on spinal cord injury without radiographic abnormality by regulating microglial phenotype polarization.

Spinal cord injury without radiographic abnormality (SCIWORA) occurs in patients with cervical spinal stenosis (CSS) following minor trauma, often leading to significant neurological deficits. While surgical decompression reduces SCIWORA risk, some patients are ineligible or reluctant to undergo surgery. Consequently, reducing the risk of SCIWORA in the absence of surgical intervention and optimizing spinal cord function recovery remain pivotal challenges.

Effects of dynamic stabilization and fusion on postoperative paraspinal muscle degeneration and lumbar function recovery.

Objective: To investigate the relationship between segmental motion and paraspinal muscle degeneration at the surgical level in patients with single-level lumbar degenerative disease (LDD) who have undergone either fusion or dynamic stabilization surgery.

Methods: Patients treated with posterolateral intertransverse fusion (PITF group), Isobar TTL dynamic internal fixation (TTL group), or Isobar EVO dynamic internal fixation (EVO group) for single-level lumbar degenerative disease (LDD) between March 2012 and September 2020 were included in the study. Cases were selected with complete records and follow-up times ranging from 2 to 5 years, taking into account the effects of postoperative muscle edema and age-related muscle degeneration.

Comprehensive curation and validation of genomic datasets for chestnut.

The Chinese chestnut (Castanea mollissima) stands out as a plant with significant ecological and economic value, excellent nutritional quality and natural resistance to pests and diseases. Recent strides in high-throughput techniques have enabled the continuous accumulation of genomic data on chestnuts, presenting a promising future for genetic research and advancing traits in this species. To facilitate the accessibility and utility of this data, we have curated and analyzed a collection of genomic datasets for eight Castanea species, including functional annotations, 213 RNA-Seq samples, and 330 resequencing samples.

Tanshinone IIA Promotes Functional Recovery After Spinal Cord Injury by Inhibiting Neuron and Oligodendrocyte Ferroptosis Through the GPX4/ACSL4 Axis.

Spinal cord injury (SCI) induces severe functional impairments and involves intricate secondary injury mechanisms. Tanshinone IIA (TIIA), a key bioactive component of Salvia miltiorrhiza, exhibits neuroprotective potential, yet its role in ferroptosis regulation post-SCI remains undefined. This study explored the protective effects and underlying mechanisms of TIIA in SCI.

Residential exposure to noise, green space, and children's language acquisition.

Background: Noise pollution has been linked to impaired development in a variety of language-related skills in laboratory settings. While studies have focused on school environments, residential noise exposure's impact remains underexplored.

Methods: We used multilevel regression models to examine the association between noise exposure measured using deterministic noise modelling and language development in kindergarten-aged children in Vancouver, Canada, between April 1, 2000 and December 31, 2005, measured through a questionnaire completed by kindergarten teachers (the Early Development Instrument).