The osteosarcoma immune microenvironment in progression: PLEK as a prognostic biomarker and therapeutic target.

Introduction: Osteosarcoma (OS) is a malignant bone tumor with high metastatic potential and poor long-term survival. The tumor immune microenvironment and metabolic reprogramming are increasingly recognized as key drivers of OS progression, yet the molecular links between these systems remain unclear. This study aimed to identify immune-metabolic biomarkers in OS, focusing on pleckstrin (PLEK) as a potential regulatory hub.

The Homocysteine S-Methyltransferase Family and Functional Analysis of Resistance to Bacterial Wilt in Peanut.

The homocysteine S-methyltransferase (HMT) family plays a crucial role in plant metabolism and stress adaptation; however, its functional characteristics in peanuts and its association with biotic stress tolerance have not been thoroughly investigated. To bridge this knowledge gap, we identified 10 AhHMT family members that exhibit significant evolutionary conservation with HMT homologues from leguminous and solanaceous species. Collinearity analyses further revealed conserved synteny between AhHMTs and orthologs in wild peanut, , soybean, and other plant species.

Insights to Resistive Pulse Sensing of Microparticle and Biological Cells on Microfluidic Chip.

Since the initial use of biological ion channels to detect single-stranded genomic base pair differences, label-free and highly sensitive resistive pulse sensing (RPS) with nanopores has made remarkable progress in single-molecule analysis. By monitoring transient ionic current disruptions caused by molecules translocating through a nanopore, this technology offers detailed insights into the structure, charge, and dynamics of the analytes. In this work, the RPS platforms based on biological, solid-state, and other sensing pores, detailing their latest research progress and applications, are reviewed.

Rural-Urban differentials in the factors associated with modern contraceptive usage among married reproductive-aged women in Hubei province, China.

Background: China's fast development is markedly impacting on migration, which has brought tremendous pressure and severe challenges to family planning services. Moreover, family planning (FP) is mostly regarded as women's responsibility by some Chinese cultures and societies. Several female contraceptives are easily accessible but only a few are known and widely used.

Lactobacillus acidophilus promotes cognitive function recovery via regulating microglial peroxisomal function in cerebral ischemia.

Cerebral ischemia causes significant mortality and is accompanied by inflammatory reactions that exacerbate damage. While the gut microbiota is linked to clinical outcomes, specific treatments and mechanisms remain unclear. Our metagenomics linked low Lactobacillus abundance to cognitive decline in patients with cerebral ischemia.

Altered glucose metabolism in B cells: Implications for the pathogenesis and treatment of autoimmune diseases.

Introduction: Metabolic dysregulation in immune cells is increasingly recognized as a contributing factor in autoimmune diseases. B lymphocytes, which play key roles in immune tolerance and autoantibody production, show altered glucose metabolism. This review examines the role of glucose metabolism in B cell function and its potential impact on autoimmune pathogenesis.

Predicting IDH and 1p/19q molecular status of gliomas with multi-b values DWI.

Background And Purpose: In the 2021 WHO Classification, the importance of molecular pathology in glioma diagnosis has been emphasized, particularly the status of isocitrate dehydrogenase (IDH) mutation and 1p/19q co-deletion. Advanced magnetic resonance diffusion-weighted imaging (DWI) including mono-exponential (Mono), intravoxel incoherent motion (IVIM), stretched exponential model (SEM) techniques are beneficial for non-invasive prediction of these molecular markers. The continuous-time random walk (CTRW) model mitigates the empirical nature of the SEM and has shown promising results in grading gliomas.

Utilizing multiple diffusion metrics in evaluation of corticospinal tract injury in patients with glioblastoma.

Objectives: This study aimed to evaluate the efficacy of diffusion tensor imaging (DTI), diffusion kurtosis imaging (DKI), neurite orientation dispersion and density imaging (NODDI), and mean apparent propagator-magnetic resonance imaging (MAP-MRI) in detecting CST injury caused by GBM and to compare their performances.

Materials And Methods: We enrolled 76 patients diagnosed with GBM with motor weakness (MW,  = 22) or normal motor (NM,  = 54). Bilateral CSTs were reconstructed, and a comparative analysis of diffusion parameters was performed based on four imaging models between affected and healthy sides.

Extraperitoneal vs transperitoneal laparoscopic cystectomy: optimized surgical techniques and long-term outcomes in a single-center retrospective cohort study.

Objective: To evaluate and compare the long-term oncologic outcomes and perioperative performance of extraperitoneal laparoscopic radical cystectomy (ELRC) versus transperitoneal laparoscopic radical cystectomy (TLRC) in patients with bladder cancer (BC).

Patients And Methods: This retrospective single-center cohort study included 298 BC patients who underwent ELRC (n = 202) or TLRC (n = 96) between January 2020 and January 2025. Primary endpoints included overall survival (OS), cancer-specific survival (CSS), progression-free survival (PFS), and recurrence-free survival (RFS).

In Vitro Fertilization and Dichorionic-Diamniotic Twin Pregnancies in Nulliparous Women: A Retrospective Cohort Study Comparing Pregnancy Outcomes Between Advanced and Younger Maternal Age Groups.

Objective: To compare pregnancy outcomes between advanced maternal age (AMA, ≥ 35 years) and younger maternal age (YMA, 20-34 years) nulliparous women with IVF-conceived dichorionic-diamniotic (DCDA) twin pregnancies.

Methods: We conducted a retrospective analysis on 141 nulliparous women undergoing cesarean delivery at The Second Nanning People's Hospital (2020-2024). Propensity score matching (PSM, 1:1, caliper width=0.

Advances and emerging trends in the tumor microenvironment of colorectal cancer liver metastasis: a bibliometric analysis.

Background: Colorectal cancer liver metastasis (CRLM) significantly reduces the survival rate of colorectal cancer patients and increases the complexity of treatment decisions. Analyzing the dynamic interplay of cellular subtypes and phenotypic characteristics in the tumor microenvironment (TME) is essential for developing effective anti-cancer therapies and improving prognosis. Consequently, this research employs bibliometric methods to systematically examine current research focus and emerging developments in the TME associated with CRLM.

Genome-wide association analysis of wheat stem traits using 55K microarrays.

Wheat lodging poses a severe threat to yield and quality, with the morphological and structural traits of the basal second internode being critical determinants of stem strength and lodging resistance. We conducted phenotypic analysis on 239 wheat varieties (lines) collected from around the world. This analysis was complemented by genotyping using the wheat 55K SNP chip.

Histogram analysis based on DTI and NODDI for differentiating atypical high-grade glioma from primary central nervous system lymphoma.

Background And Purpose: Distinguishing between high-grade glioma (HGG) and primary central nervous system lymphoma (PCNSL) is of paramount clinical importance, as these entities necessitate substantially different therapeutic approaches. The differential diagnosis becomes particularly challenging when HGG presents without characteristic magnetic resonance imaging (MRI) features, making it difficult to differentiate from PCNSL. The diffusion tensor imaging (DTI) and neurite orientation dispersion and density imaging (NODDI) offer quantitative assessments of water molecule diffusion within tissues, thereby providing potential means to characterize microstructural differences between HGG and PCNSL.

Biphasic liquid metal composites as soldering systems for robust soft-rigid interfacing in stretchable hybrid electronics.

Stretchable hybrid electronics are integral in numerous domains such as healthcare, soft robotics, and human-machine interfaces. However, their development encounters significant challenges under mechanical deformation, primarily due to stress concentration at soft-rigid interfaces. Structural engineering or rigid-filler composites, as conventional soldering solutions, face critical limitations including restricted strain tolerance and inherent trade-offs between conductivity and stretchability.

Giant adrenal calcification: rare case report and literature review.

We report the largest documented case of idiopathic giant adrenal calcification in a middle-aged woman, incidentally detected during imaging. Preoperative CT revealed an 8.5 × 7.

Phloroglucinol Oligomers from as Novel Anti-Hantavirus Replication Agents.

Zoonotic viral diseases have continued to threaten global public health in recent decades, with rodent-borne viruses being significant contributors. Infection by rodent-carried hantaviruses (HV) can result in hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS) in humans, with varying degrees of morbidity and mortality. However, no Food and Drug Administration (FDA) vaccines or therapeutics have been approved for the treatment of these diseases.

Effects of Micro-Topography on Soil Nutrients and Plant Diversity of Artificial Shrub Forest in the Mu Us Sandy Land.

In ecological restoration of arid/semi-arid sandy lands, micro-topographic variations and artificial shrub arrangement synergistically drive vegetation recovery and soil quality improvement. As a typical fragile ecosystem in northern China, the Mu Us Sandy Land has long suffered wind erosion, desertification, soil infertility, and vegetation degradation, demanding precise vegetation configuration for ecological rehabilitation. This study analyzed soil nutrients, plant diversity, and their correlations under various micro-topographic conditions across different types of artificial shrub plantations in the Mu Us Sandy Land.

GutGPT: A multidimensional knowledge-enhanced large language model for gastrointestinal medicine.

Background: Gastrointestinal (GI) diseases are common, chronic conditions that require personalized, long-term management, placing a heavy burden on traditional healthcare systems. While large language models (LLMs) offer potential for supporting patient care with personalized and empathetic guidance, existing models often lack domain-specific knowledge in GI diseases and suffer from issues like slow convergence and overfitting.

Methodology: We first construct a high-quality GI disease QA dataset comprising 191,615 entries from diverse sources: real-world doctor-patient dialogues, medical knowledge graphs, medical guidelines, and Chinese medical licensing exam data.

Finite-time active disturbance rejection control scheme for quadrotor attitude tracking.

In this paper, a finite-time active disturbance rejection control (FADRC) framework for quadrotor attitude tracking is presented. The FADRC framework is developed for two cascaded loops (inner-loop and outer-loop) to control the attitude angle and angular velocity, respectively. Two nonlinear extended state observers (NESOs) are devised to actively estimate and compensate for total disturbances, including internal uncertainties and external disturbances.

Preeclampsia: The silent catalyst for autism spectrum disorder in offspring.

Preeclampsia, a pregnancy-specific disorder characterized by hypertension and proteinuria, has been hypothesized to be associated with the development of autism spectrum disorder (ASD) in offspring. This review synthesizes epidemiological evidence and examines biological mechanisms, proposing a significant association between preeclampsia and ASD. The analysis demonstrates an increased risk of ASD in offspring of mothers with preeclampsia, with early-onset and severe preeclampsia likely exerting a more significant effect.

Whole-genome resequencing to investigate the genetic diversity and the molecular basis underlying key economic traits in indigenous sheep breeds adapted to hypoxic environments.

Under the combined effects of long-term natural selection and artificial domestication, Tibetan sheep on the Qinghai-Tibet Plateau have evolved distinct ecotypes to survive extreme high-altitude conditions, including hypoxia, cold, and low oxygen levels. These ecotypic variations not only serve as an ideal model for studying plateau livestock adaptation but also harbor valuable genetic diversity. However, the lack of comprehensive genetic analyses on their adaptive and phenotypic traits has hindered the effective conservation and utilization of these resources.

Chitosan-based nanomaterials: Pioneering a review in veterinary medicine applications.

With the rapid development of nanotechnology, chitosan (CS)-based materials have attracted great attention in the field of veterinary medicine. CS, a natural biopolymer with a modifiable structure and abundant functional groups, is widely utilized in biomedicine, pharmaceuticals, and food applications due to its nontoxic, biocompatible, antibacterial, and biodegradable properties. In this paper, the research and application of CS-based materials in veterinary medicine field in recent years are analyzed and summarized.

CENPT prevents renal cell carcinoma against ferroptosis by enhancing the synthesis of glutathione.

Cancer is characterized by chromosomal instability (CIN), which leads to tumor heterogeneity and other malignant features. CIN is caused by abnormal centromere and kinetochore function, which results in aneuploidy, rearrangements, and micronucleus production. Centromere and kinetochore gene misexpression plays a vital role in tumor progression.

Effects of Nutritional Supplements on Endurance Performance and Subjective Perception in Athletes Exercising in the Heat: A Systematic Review and Network Meta-Analysis.

: This study aimed to evaluate the efficacy of various nutritional supplements in enhancing endurance performance and subjective thermal perception in athletes exposed to high-temperature environments through a systematic review and network meta-analysis. : A comprehensive search was conducted in PubMed, Embase, Web of Science, Cochrane Library, and EBSCOhost from inception to January 2025. Studies were included if they evaluated the effects of nutritional supplements on either endurance performance or subjective thermal perception in athletes under heat stress.