Development of an ELISA for NNV-Specific Antibody Detection in Grouper Hatcheries in China.

Grouper ( sp.) is one of the most economically important aquaculture species in China and Southeast Asian countries. Nervous necrosis virus (NNV) infection causes significant mortality in grouper aquaculture.

Characterization of Whey Protein Isolate-Soymilk Complexes Modified by Transglutaminase and Their Application inYuba Film.

Transglutaminase (TGase) improves protein structure by facilitating cross-linking reactions. However, the effects of TGase on the physicochemical properties of whey protein isolate (WPI)-soymilk complexes and their applications in yuba remain unclear. Therefore, the impacts of TGase concentration on the free sulfhydryl content, free amino content, particle size, and structure of WPI-soymilk complexes and their film-forming properties were studied.

Comparative Analysis of Roller Milling Strategies on Wheat Flour Physicochemical Properties and Their Implications for Microwave Freeze-Dried Instant Noodles.

The milling process is a critical technological step that regulates wheat flour characteristics and ultimately determines end-product quality. This study systematically evaluated the effects of three key milling parameter adjustments in a laboratory-scale roller mill-double sifting (2S), double break milling (2BM), and increased roll gap (IRG)-on the physicochemical properties of wheat flour and the quality of microwave freeze-dried non-fried instant noodles. The results demonstrated that milling processes significantly influenced the particle size and composition of flour.

meningitis in an immunocompetent patient diagnosed by metagenomic next-generation sequencing: a case report.

Cerebral nocardiosis caused by represents a rare and diagnostically challenging infectious disease, predominantly affecting immunocompromised patients. This opportunistic infection may also pose life-threatening risks to immunocompetent individuals. The diagnostic process is frequently complicated by the absence of distinctive clinical manifestations and technical limitations inherent to conventional microbiological detection methods, which collectively impede the acquisition of definitive pathogenic evidence, thereby resulting in diagnostic delays.

The expression pattern and role of circulating CXCR5 T cells in children with newly diagnosed immune thrombocytopenia.

Introduction: Immune thrombocytopenia (ITP) is the most common bleeding disorder in children. Tfh cells play a crucial role in the pathogenesis of ITP by promoting the production of anti-platelet autoantibodies. Recent studies have shown that CXCR5 T cells not only possess "Tfh-like" cell functions but also can induce Tfh cell differentiation.

Glancing Beyond Patch: Spatial Contextual Cues for 3D Neuron Segmentation.

Accurate segmentation of neurons in 3D fluorescence microscopy images is essential for advancing neuroscience. Prevalent methods split a volume into patches and process each patch separately due to computational resource limitations. However, they fail to capture global neuronal morphology across multiple patches, which results in discontinuous segmentation and poses a challenge for subsequent neuronal reconstruction.

Enhancing 3D Printing Performance of Yellow-Flesh Peach Gels: The Role of Whey Protein Isolate in Printability, Rheological Properties, and Structural Characteristics.

The utilization of 3D printing technology in the food sector enables innovative product customization through the design of intricate shape, offering new potential for yellow peach product development. However, the 3D printing performance of pure yellow-flesh peach gel is suboptimal. This study aims to address this gap by exploring functional additives to optimize 3D printing performance for peach-based materials.

Intrauterine fetal death due to Brucella melitensis infection: a case report and literature review.

Brucellosis is a zoonotic disease caused by Brucella species In pregnant women, this disease may lead to adverse obstetric outcomes, such as abortion, preterm delivery, intrauterine fetal death (IUFD), and congenital brucellosis. However, only a few studies have reported IUFD due to Brucella. In our case, one woman residing in Guangxi Province, China-a non-endemic region-developed symptoms 11 months after exposure.

Preservation effect of plant essential oil-KGM composite coating materials on the tomatoes.

Tomatoes with rich in nutrients are very popular for humans, but they are extremely perishable during storage and transportation, which may limit their economic and nutritional value. In this research, the suitable concentration of essential oil and soaking time were investigated and selected by measuring the changes of tomatoes' weight loss ratio and hardness with single factor experiment. Subsequently, the Box-Behnken experimental design model from Response Surface Methodology (RSM) was employed to optimize key process parameters for tomato preservation, with three independent variables selected: cinnamon essential oil concentration, konjac glucomannan (KGM) concentration, and immersion time.

Study on the physicochemical properties and gelation characteristics of pea protein isolate modified by ultrasonic-assisted succinylation.

Ultrasound could facilitate the succinylation of protein and improve the function of succinylated protein. However, the effects of ultrasound-assisted succinylation on the physicochemical property and gelation characteristics of PPI were scarely reported. Therefore, this research studied the effect of succinylation with different ultrasonic power on the physicochemical properties and gelation characteristics of succinylated pea protein isolate (PPI).

Clinical and Antibiotic Resistance Features for Extended-Spectrum Betalactamase-Producing and Bloodstream Infections and Predictors of Poor Prognosis in Neonatal Patients.

Background: Neonatal bloodstream infections (BSIs) caused by extended-spectrum β-lactamase (ESBL-E) are associated with high rates of morbidity and mortality. This study aimed to describe the clinical characteristics, profiles of antibiotic susceptibility, and risk factors associated with BSIs caused by (ESBL-EC) and (ESBL-KP), and identify risk factors for poor prognosis in neonatal patients with or BSIs.

Methods: In the period from January 2017 to December 2023, a retrospective case-control study was conducted at Guangxi Children's Hospital in China.

Histogram analysis based on DTI and NODDI for differentiating atypical high-grade glioma from primary central nervous system lymphoma.

Background And Purpose: Distinguishing between high-grade glioma (HGG) and primary central nervous system lymphoma (PCNSL) is of paramount clinical importance, as these entities necessitate substantially different therapeutic approaches. The differential diagnosis becomes particularly challenging when HGG presents without characteristic magnetic resonance imaging (MRI) features, making it difficult to differentiate from PCNSL. The diffusion tensor imaging (DTI) and neurite orientation dispersion and density imaging (NODDI) offer quantitative assessments of water molecule diffusion within tissues, thereby providing potential means to characterize microstructural differences between HGG and PCNSL.

Pericapsular Nerve Group Block in Combination with Lateral Femoral Cutaneous Nerve and Sacral Plexus Blocks for Hip Fracture: A Case Series.

Background: Regional anesthetic techniques for hip fracture are critical for pain control, reducing perioperative adverse events, and minimizing postoperative opioid use. This case series investigates the use of a novel triple-block protocol-ultrasound-guided pericapsular nerve group (PENG) block, lateral femoral cutaneous nerve (LFCN) and sacral plexus blocks-in patients with hip fractures, focusing on its feasibility as an anesthetic strategy for high-risk hip fracture patients.

Case Presentation: Five frail elderly patients with significant comorbidities and contraindications to neuraxial anesthesia underwent bipolar femoral head replacement surgery.

Hydrogen-Bonded Perovskite Heterojunction Photocatalytic Membrane with Efficient Proton Supply for Boosting CO Methanation.

Efficient proton supply and enhanced *CO intermediate adsorption are crucial strategies for boosting CO methanation. Herein, we construct a hydrogen-bonded (H-bonded) photocatalytic membrane by integrating a MAPbBr/cyano-g-CN (MPB/CN-g-CN) heterojunction with ethyl cellulose (ECE) binder through a simple sol-gel process, achieving significantly enhanced CO methanation efficiency. ECE functions as a novel proton source, with hydroxyl (-OH) groups that are readily oxidized by photogenerated holes, resulting in efficient proton supply compared to HO.

Effects of Black Garlic Polyphenols on the Physicochemical Characteristics, Antioxidant Activity, and Sensory Evaluation of Yogurt.

Yogurt fortified with polyphenols, as a new type of functional yogurt, exhibits high quality and good antioxidant activity. However, the effects of black garlic polyphenols (BGP) on the quality of solidified yogurt have been scarcely reported. Therefore, the effects of different levels (0.

Unraveling the role of cumulative triglyceride-total cholesterol-body weight index in stroke development: evidence from the CHARLS cohort.

Background: This study investigated the association between the cumulative triglyceride-total cholesterol-body weight index (TCBI) and the risk of stroke among middle-aged and older adults, focusing on hypertension as a potential mediator.

Methods: Data from 5,598 participants aged ≥ 45 years in the China Health and Retirement Longitudinal Study were analyzed over a median follow-up of 57.2 months.

Circulating Level of Oxidized Low-Density Lipoprotein Correlates with Cerebral Blood Flow in Patients with White Matter Hyperintensities.

Background: Oxidized low-density lipoprotein (ox-LDL) has been shown to promote atherosclerosis, but data have been limited on its association with white matter hyperintensities (WMH).

Objective: To assess the relationship of ox-LDL levels with WMH.

Methods And Material: This study included 45 patients with WMH, matched for age, sex, and education with 45 healthy controls (HC).

Dupilumab-induced Eosinophilic Granulomatosis with Polyangiitis Complicated by Peripheral Neuropathic Pain: a Case Report and Literature Review.

Purpose: Eosinophilic Granulomatosis with Polyangiitis (EGPA) is a rare vasculitis characterized by increased eosinophils in human tissues and peripheral blood. In this case, we present a 53-year-old female patient with EGPA. By this case and literature review, we want to explain the early manifestations, diagnosis, and management of EGPA, which will help clinicians to understand the disease and attach importance to the possibility of dupilumab-induced EGPA, to improve the early diagnosis rate of EGPA, and reduce misdiagnosis and missed diagnosis.

Trends and emerging frontiers of photodynamic therapy for non-melanoma skin cancer (1979-October 2024): a bibliometric analysis.

Introduction: Photodynamic therapy (PDT) is a non-invasive treatment modality for non-melanoma skin cancer (NMSC). This study aims to use bibliometric analysis to explore the research trends and development in the field of PDT for NMSC, identifying key hotspots and emerging directions.

Methods: This study conducted a comprehensive literature search in the Web of Science Core Collection database and applied bibliometric analysis and visualization using tools such as VOSviewer, CiteSpace, and the R package "bibliometrix".

Ultrathin formvar film protective layer via simple dipping strategy for ultra-stable zinc-metal anodes.

Aqueous Zn ion batteries (ZIBs) have become a strong competitor in the field of large-scale energy storage. However, surface side reactions, such as Zn corrosion, hydrogen evolution reaction, and dendrite growth, lead to low reversibility of Zn anode. This study introduces a time-saving and simple solution-dipping method to address above issues by spreading ultrathin Poly (vinyl formal) (PVF) protective layer on the surface of Zn anode (denoted as PVF@Zn).

Ablation of macrophage transcriptional factor FoxO1 protects against ischemia-reperfusion injury-induced acute kidney injury.

Acute kidney injury (AKI) has high morbidity and mortality, but effective clinical drugs and management are lacking. Previous studies have suggested that macrophages play a crucial role in the inflammatory response to AKI and may serve as potential therapeutic targets. Emerging evidence has highlighted the importance of forkhead box protein O1 (FoxO1) in mediating macrophage activation and polarization in various diseases, but the specific mechanisms by which FoxO1 regulates macrophages during AKI remain unclear.

Neutrophil extracellular traps mediate inflammation and coagulation dysregulation in primary blast lung injury.

Primary blast lung injury (PBLI) manifests as respiratory distress and hemoptysis syndrome resulting from exposure to blast waves, yet its pathogenesis remains incompletely understood, leading to a dearth of effective treatment options. Neutrophil extracellular traps (NETs) may play important roles in acute lung injury. However, the role of NETs in PBLI was unclear.

Edible beeswax-flaxseed oil nanostructured lipid carriers for improved β-sitosterol bioaccessibility: structural advantages and controlled release in dynamic digestion.

This study developed nanostructured lipid carriers (NLCs) using edible beeswax and flaxseed oil to encapsulate β-sitosterol. Structural analysis revealed the formation of a less ordered lipid matrix with reduced crystallinity compared to pure beeswax, contributing to a high encapsulation efficiency of 92 %. Optimal formulation (beeswax: flaxseed oil 3:2, Tween 80: Polyglycerol fatty acid(PGE) 4:1 totaling 7 % emulsifier) and processing parameters (10 min homogenization, 200 W ultrasonic power for 4 min) yielded stable nanoparticles maintaining physical stability for 40 days across varying pH and temperature.

Effects of Sodium Hexametaphosphate on the Gel Properties and Structure of Glutaminase-Transaminase-Crosslinked Gelatin Gels.

Gelatin is a commonly used protein-based hydrogel. However, the thermo-reversible nature of gelatin makes it unstable at physiological and higher temperatures. Therefore, this study adopted phosphates and glutaminase transaminase (TG) to modify gelation and studied the effects of combining sodium hexametaphosphate (SHP) and TG on the structure and gel properties of TG-crosslinked gelatin.

Mbnl1 Protects Against Cerebral Ischemia-Reperfusion Injury by Modulating Microglia/Macrophage Polarization via NF-κB Pathway.

Activated and polarized microglia regulate neuroinflammatory responses and programmed cell death processes in ischemic stroke. Although the inactivation of muscleblind-like 1 (Mbnl1) is known to cause structural defects in the brain, its role in microglial apoptosis and polarization remains unclear. This study aims to explore the mechanism of Mbnl1 in ischemic stroke, particularly its role in the regulation of microglial apoptosis and polarization, as well as its impact on neuroinflammatory responses and cognitive dysfunction.