meningitis in an immunocompetent patient diagnosed by metagenomic next-generation sequencing: a case report.

Cerebral nocardiosis caused by represents a rare and diagnostically challenging infectious disease, predominantly affecting immunocompromised patients. This opportunistic infection may also pose life-threatening risks to immunocompetent individuals. The diagnostic process is frequently complicated by the absence of distinctive clinical manifestations and technical limitations inherent to conventional microbiological detection methods, which collectively impede the acquisition of definitive pathogenic evidence, thereby resulting in diagnostic delays.

The expression pattern and role of circulating CXCR5 T cells in children with newly diagnosed immune thrombocytopenia.

Introduction: Immune thrombocytopenia (ITP) is the most common bleeding disorder in children. Tfh cells play a crucial role in the pathogenesis of ITP by promoting the production of anti-platelet autoantibodies. Recent studies have shown that CXCR5 T cells not only possess "Tfh-like" cell functions but also can induce Tfh cell differentiation.

Circulating Level of Oxidized Low-Density Lipoprotein Correlates with Cerebral Blood Flow in Patients with White Matter Hyperintensities.

Background: Oxidized low-density lipoprotein (ox-LDL) has been shown to promote atherosclerosis, but data have been limited on its association with white matter hyperintensities (WMH).

Objective: To assess the relationship of ox-LDL levels with WMH.

Methods And Material: This study included 45 patients with WMH, matched for age, sex, and education with 45 healthy controls (HC).

[Water Quality Evolution in the Guishui River Section of Guanting Reservoir in Recent Years].

Based on manual and automatic monitoring data of water quality at the Gujiaying section of the upstream of Guanting Reservoir （Guishui River section） from 2017 to 2023， this study comprehensive applied the Mann Kendall statistical test， STL trend decomposition， grey prediction model， and emission inventory statistics and other analysis methods， combined with literature research， and systematically revealed the water quality evolutions， influencing factors， and the impact of epidemic control and rainstorm events on water quality in the upper reaches of Guanting Reservoir. The results showed that： ① The period from 2017 to 2023 was the best time for the upstream inflow water quality of Guanting Reservoir in 50 years. Since Guanting Reservoir was restored as a backup water source in 2007， the inflow water quality was significantly improved， rising from Class Ⅳ to Class Ⅲ， reaching Class II in some months.

Novel SPTB Variations Cause Hereditary Spherocytosis With Cholangiolithiasis and Severe Intrahepatic Cholestasis.

Background: Hereditary spherocytosis (HS) is a chronic non-immune hemolytic anemia caused by congenital defects in the erythrocyte membrane. Gene variations can lead to HS, and the SPTB gene variation is one of them. However, HS with cholangiolithiasis and extremely intrahepatic cholestasis had been rarely discussed as a phenotype caused by SPTB gene variation, and the pathogenic mechanism of this gene variation is still unclear.

Eleven undescribed sesquiterpenoids with multiple skeleton types and cytotoxicity from the South China Sea soft coral Lemnalia sp.

Marine organisms have great potential for medical and bioproducts applications, with corals having been in the spotlight as an important research target for the discovery of new bioactive marine natural products. Eleven undescribed sesquiterpenoids, named lemnalinoids A-K (1-11), and one known sesquiterpenoid (12) were isolated from the South China Sea soft coral Lemnalia sp. The structures and absolute configurations of new compounds were elucidated by extensive analysis of spectroscopic data, single crystal X-ray diffraction, quantum chemical calculations and TDDFT-ECD calculations.

Early Stage Morphogenesis of Transverse Tubules in Rat Cardiomyocytes: The Role of pBIN1.

Background: Transverse tubules (TTs) are tubular invaginations of myocyte membrane forming junctions with sarcoplasmic reticulum and are essential for excitation-contraction coupling. Although it is known that TTs begin to develop 2 weeks after birth in rodent cardiomyocytes, the spatial profile and molecular mechanisms of TT morphogenesis are not clear. Understanding the molecular mechanism of TT morphogenesis may provide potential solutions for TT loss in pathogenic conditions such as hypertrophy and heart failure.

Commonalities between thyroid disease and systemic lupus erythematosus (SLE).

Systemic lupus erythematosus (SLE) is characterized by multiple organ involvements. One notable manifestation of SLE is thyroid disease, which has been documented in various studies with significant variability in prevalence and presentation. Observational studies have indicated that thyroid dysfunction, particularly autoimmune thyroid disease (AITD) characterized by hypothyroidism, is commonly associated with SLE.

Factors influencing the diversity of terrestrial plant communities in China.

The mechanism of biodiversity formation and maintenance is an important topic in ecological research. To reveal the ecological processes affecting plant diversity formation of terrestrial ecosystems in China, we conducted a meta-analysis to investigate the dominant factors affecting plant diversity of different growth forms in different climatic zones, including climate, soil, and topography. We collected data from 441 published papers, and constructed multiple regression models.

Theoretical study of ammonia synthesis catalysed by trimetallic clusters with or without a sumanene support.

DFT calculations were utilized to explore the electrocatalytic nitrogen reduction reaction (NRR) mechanisms catalyzed by trimetallic clusters M (M = Ti, Zr, V, and Nb), both unsupported and supported by bowl-shaped sumanene. The substrate enhanced N adsorption and activation but hindered hydrogenation due to more negative adsorption energies. The substrate promoted hydrogenation of nitrogen, reducing the interference of the hydrogen evolution reaction (HER) and enhancing the NRR selectivity.

[Phosphorus Removal from Constructed Wetlands： Efficiency, Limitations, and Intensification Measures].

Excessive phosphorus can easily lead to the eutrophication of water bodies and affect the growth of aquatic plants and animals； therefore, it is particularly important to pay attention to and control phosphorus in sewage. Constructed wetlands have become a green and economical way to remove phosphorus from sewage. The research status of phosphorus removal in constructed wetland systems in recent years was summarized here, including the removal mechanism, influencing factors, and strengthening measures.

Responses of radial growth of different tree species to abrupt temperature change in the northern Greater Khingan Mountains, China.

We constructed standardized chronologies of tree-ring width of and var. , the main tree species in the Mangui region of the northern Greater Khingan Mountains based on dendrochronology methods, we explored the responses of radial growth of the two species to climate change. The results showed that the radial growth of was mainly limited by temperature, which was significantly negatively related to mean temperature in October of the last year and that in March and May-August of the present year.

Thyroid hormones in systemic lupus erythematosus: The catalyst for disease progression?

Objective: The study aimed to investigate the impact of varying thyroid function statuses on clinical and laboratory indicators in patients with systemic lupus erythematosus (SLE).

Methods: A retrospective analysis was conducted on 258 patients with SLE, who were stratified according to thyroid function, renal involvement, and disease activity. The predictive value of thyroid hormones was evaluated using a receiver operating characteristic (ROC) curve.

Na-K-ATPase/GLT-1 interaction participates in EGCG protection against cerebral ischemia-reperfusion injury in rats.

Background: In China, stroke is the primary cause of adult death and disability. Because of the increased rate of blood vessel reperfusion, it is important to prevent cerebral ischemia-reperfusion injury, in which glutamate (Glu) excitotoxicity plays a critical role. The most important Glu transporter, GLT-1, is essential for the regulation of Glu, which is dependent on Na-K-ATPase (NKA)-induced ion concentration gradient differences.

Ecological restoration orientated application and modification of constructed wetland substrates.

Constructed wetlands (CWs) have gained recognition as an environmentally friendly and cost-efficient option for treating municipal, industrial, and agricultural wastewater. They treat wastewater by harnessing the combined action of physical, chemical, and biological processes within substrates, plants, and microorganisms, with substrates exerting the greatest influence on the life cycle and purification efficiency of the system. This review provides an in-depth discussion on the development and performance of various substrate types used in CWs, including natural materials, ore-based materials, biomass materials, waste materials, and modified and novel materials.

Identification of lu'an Guapian at different picking periods by using excitation-emission matrix fluorescence spectroscopy coupled with chemometrics.

Lu'an Guapian (LAGP) is a renowned green tea, with its price significantly higher when picked before the Qingming Festival compared to after, posing risks of confusion and counterfeiting. This study proposed using an excitation-emission matrix (EEM) fluorescence method combined with chemometrics for rapid identification of tea picked before and after Qingming Festival. Firstly, the differences among the EEM fingerprints of different tea samples were analyzed using the alternating trilinear decomposition (ATLD) algorithm.

A Nonessential Sfp-Type Phosphopantetheinyl Transferase Contributes Significantly to the Pathogenicity of .

4'-Phosphopantetheinyl transferases (PPTases) play important roles in the posttranslational modifications of bacterial carrier proteins, which are involved in various metabolic pathways. Here, we found that and encoded a functional AcpS-type and Sfp-type PPTase, respectively, in GMI1000, and both are capable of modifying AcpP1, AcpP2, AcpP3, and AcpP5 proteins. is located on the megaplasmid, which does not affect strain growth and fatty acid synthesis but significantly contributes to the virulence of and preferentially participates in secondary metabolism.

Clinicopathological characteristics and prognosis of Epstein-Barr virus-associated gastric cancer.

Objective: Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC) is a distinct molecular subtype of gastric cancer (GC). At present, the clinical characteristics and prognostic implications of EBV infection and the potential clinical benefits of immune checkpoint blockade in GC remain to be clarified. Hence, this study was designed to analyze the clinical and pathological characteristics of GC patients with varying EBV infection states and compare their overall survival (OS).

A robust transformer-based pipeline of 3D cell alignment, denoise and instance segmentation on electron microscopy sequence images.

Germline cells are critical for transmitting genetic information to subsequent generations in biological organisms. While their differentiation from somatic cells during embryonic development is well-documented in most animals, the regulatory mechanisms initiating plant germline cells are not well understood. To thoroughly investigate the complex morphological transformations of their ultrastructure over developmental time, nanoscale 3D reconstruction of entire plant tissues is necessary, achievable exclusively through electron microscopy imaging.

The Spatial Niche and Influencing Factors of Desert Rodents.

Resource partitioning may allow species coexistence. Sand dunes in the typical steppe of Alxa Desert Inner Mongolia, China, consisting of desert, shrub, and grass habitats, provide an appropriate system for studies of spatial niche partitioning among small mammals. In this study, the spatial niche characteristics of four rodents, , , and , and their responses to environmental changes in the Alxa Desert were studied from 2017 to 2021.

Clinicopathological features, psychological status, and prognosis of 33 patients with occult breast cancer.

Background: Occult breast cancer (OBC) has traditionally been considered to be a carcinoma of unknown primary origin with a favorable prognosis and can be treated as stage II-III breast cancer. Due to the small number of cases and limited clinical ex-perience, treatments vary greatly around the world and no standardized treat-ment has yet been established.

Aim: To investigate the clinicopathological features, psychological status and prog-nostic features of patients with OBC.

Anti-complement factor H (CFH) autoantibodies could delay pristane-induced lupus nephritis.

Purpose: Anti-complement factor H (CFH) autoantibodies could be detected in lupus and its significance remained to be elucidated. Herein, we aimed to explore the roles of anti-CFH autoantibodies based on pristane-induced lupus mice.

Methods: Twenty-four female Balb/c mice were randomly divided into four groups, with one group injected with pristane (pristane group), one group with pristane and then human CFH (hCFH) (pristane-CFH group) 3 times, and the other two as vertical controls, PBS group and PBS-CFH group.

A Porous π-Stacked Self-Assembly of Cup-Shaped Palladium Complex for Iodine Capture.

Acquiring adsorbents capable of effective radioiodine capture is important for nuclear waste treatment; however, it remains a challenge to develop porous materials with high and reversible iodine capture. Herein, we report a porous self-assembly constructed by a cup-shaped Pd complex through intermolecular ··· interactions. This self-assembly features a cubic structure with channels along all three Cartesian coordinates, which enables it to efficiently capture iodine with an adsorption capacity of 0.