Correction: Monocyte count combined with GTVnx is an independent prognostic factor in non-metastatic nasopharyngeal carcinoma receiving radiotherapy.

[This corrects the article DOI: 10.3389/fonc.2025.

Enhancement of High-Temperature Triboelectric Performance of Polyimide Nanofibers by Introducing Fluorinated Monomers.

Triboelectric nanogenerators (TENGs) are typically constrained to operate below 200 °C due to the thermionic emission effect and material degradation at high temperatures. Herein, high-temperature-resistant fluorinated polyimide nanofibers (4,4'-(hexafluoroisopropylidene) diphthalic anhydride-4,4'-oxidianiline/2,2″-bis(trifluoromethyl)benzidine, 6FDA-ODA/TFDB) were designed to mitigate the thermionic emission effect through the introduction of trifluoromethyl (-CF) groups. 6FDA-ODA/TFDB nanofibers exhibited a fine fiber structure and a large highest occupied molecular orbital-lowest unoccupied molecular orbital (HOMO-LUMO) gap, which enhanced its effective contact area and maintained more localized states for charge transfer.

GDF15 Ameliorates Deoxynivalenol-Induced Anemia by Resolving Ribosomal Stress-Mediated Erythropoietic Arrest.

Deoxynivalenol (DON) is a prevalent trichothecene mycotoxin that contaminates global food supplies, posing significant health risks; however, targeted therapies against DON are scarce. Although DON-induced anemia is well-documented, the underlying mechanisms remain unclear. In this study, the effects of DON on erythropoiesis are examined in detail using complementary murine models and human primary erythroid cultures.

Single-cell, spatial and bulk transcriptome data analysis revealed LINC00467-mediated Sertoli cell ferroptosis is a potential therapeutic target and biomarker for azoospermia.

Human spermatogenesis is an important physiological process related to programmed cell death. However, which type of programmed cell death playing a key role in normal and abnormal human spermatogenesis remains obscure. This study integrated single-cell, bulk RNA and spatial transcriptome data analysis and found that the ferroptosis signal plays a potential role in spermatogenesis and significantly elevate in testicular samples from humans with non-obstructive azoospermia (NOA) due to various factors.

Epidemiological characteristics and environmental contamination of monkeypox in Beijing's Chaoyang district: Field evidence from 2023 to 2024.

Objectives: The mpox outbreak in Beijing's Chaoyang District after late May 2023, this study aims to evaluate the epidemiological characteristics and environmental contamination of this outbreak.

Methods: A field investigation was conducted to evaluate the epidemiological characteristics of mpox cases and their living environments in Chaoyang District, Beijing, between May 2023 and May 2024. Laboratory testing, epidemiological description, and statistical analysis were conducted subsequently.

Development of a Machine Learning Model Integrating Pathomics and Clinical Data to Predict Axillary Lymph Node Metastasis in Breast Cancer: A Two-Center Study.

Background: Accurately assessing the status of axillary lymph nodes (ALNs) is essential for devising optimal surgical plans and making informed treatment decisions in breast cancer (BC) patients.

Aims: This study aims to develop an innovative nomogram based on pathomics to preoperatively predict ALN metastasis (ALNM) in BC.

Methods And Results: Our study performed a retrospective analysis on digital hematoxylin and eosin (H&E)-stained images obtained from 407 patients across two institutions who were allocated into a training cohort (TC; n = 203), an internal validation cohort (IVC; n = 136), and an external validation cohort (EVC; n = 68).

Comparing and optimizing protein extraction methods with different lysis buffers for the analyses of human fecal microbiome via metaproteomics approach.

Metaproteomic analysis offers critical insights into gut microbiome function; however, efficient microbial protein extraction from fecal samples remains challenging due to the complexity of different types of bacterial cell walls in the microbiome. In this study, we systematically compared three representative detergent-based lysis buffers (sodium dodecyl sulfate_urea, dodecyl β-D-maltoside_urea, sodium dodecyl sulfate_ dodecyl β-D-maltoside_urea) for metaproteomics sample preparation. After multiple levels of analyses, we identified SDS_DDM_urea as the most efficient option for extracting diverse microbial proteins, peptides, and identifying microbial species.

SALSA: a novel flow cytometry assay to detect ascorbate at the single-cell level.

Ascorbate (AA) is an essential antioxidant and enzymatic cofactor with emerging roles in epigenetic regulation, redox biology, and immune function. However, single-cell quantification of intracellular AA has remained technically challenging. Here, we present SALSA (Single-cell Ascorbate Level Sensing Assay), a novel flow cytometry-based method that enables sensitive, specific detection of intracellular AA at the single-cell level.

Integrated Transcriptome and Metabolome Analysis Provides Insights into the Low-Temperature Response in Sweet Potato ( L.).

Background/objectives: Sweet potato is a tropical and subtropical crop and its growth and yield are susceptible to low-temperature stress. However, the molecular mechanisms underlying the low temperature stress of sweetpotato are unknown.

Methods: In this work, combined transcriptome and metabolism analysis was employed to investigate the low-temperature responses of two sweet potato cultivars, namely, the low-temperature-resistant cultivar "X33" and the low-temperature-sensitive cultivar "W7".

Radical surgery versus definitive radiotherapy in treatment of locoregional upper esophageal squamous cell carcinoma.

Backgrounds: Current consensus guidelines lack robust evidence to advocate the optimal treatment approach for locoregional upper esophageal squamous cell carcinoma (L-UESCC). The purpose of this study was to conduct an in-depth investigation of the treatment patterns, outcomes, and prognostic factors among patients with L-UESCC.

Methods: Consecutive patients diagnosed with L-UESCC who underwent curative-intent treatment at two cancer centers from January 2013 to December 2018 were enrolled in this study.

Global burden of peripheral arterial disease and its risk factors, 1990-2021.

Background: Lower extremity peripheral arterial disease (PAD) is an atherosclerotic condition affecting the peripheral vasculature, resulting in arterial stenosis or occlusion in the lower limbs. The focus is on understanding the epidemiology of this often-overlooked and debilitating disease.

Methods: Data on PAD were retrieved from the GBD 2021.

Submillimeter-length single-helix chiral grating in high-NA fiber for robust third-order OAM generation.

We demonstrate a submillimeter-length single-helix chiral grating embedded in a high-numerical-aperture single-mode fiber (HNA-SMF) for efficient generation of third-order orbital angular momentum (OAM), specifically the OAM mode. This design facilitates enhanced coupling of higher azimuthal modes due to significant perturbations arising from both the geometric effect of the thin-core offset under high-NA conditions and the elasto-optic effect induced by intense helical stress with a small twist pitch. As a result, we achieve an unprecedented device length of 0.

Insights into heterogenetic mechanism in physicochemical driving factors, microbial community and volatile metabolites during high-temperature Daqu fermentation by traditional stacking and shelf stacking.

Background: Research on shelf-stacking fermentation is growing, with the aim of reducing labor and improving Daqu stability, but it still falls short compared to traditional stacking fermentation, especially for high-temperature Daqu (HTD). The heterogenetic mechanisms underlying+ these two Daqu remain unclear.

Results: The improved micro-oxygen environment in shelf-stacking fermentation significantly reduced acidity and increased liquefaction and esterification activities compared to traditionally stacked HTD (HTD-T).

Multi-omics to evaluate the protective mechanisms during Akkermansia muciniphila treatment of Candida albicans colonization and subsequent infection.

Akkermansia muciniphila (AKK, A. muciniphila) fortifies the intestinal barrier, inhibits the colonization of pathogenic bacteria, and protects the host's health. Nevertheless, the existing literature offers inadequate evidence to ascertain whether A.

Deciphering genetic associations with blood pressure in peripheral blood mononuclear cells through single-cell transcriptomic profiling.

Functional genes within genomic loci associated with blood pressure (BP) identified by genome-wide association studies (GWASs) are cell type-specific. This study aims to identify such genes in immune cells by employing single-cell transcriptomic analysis in peripheral blood mononuclear cells (PBMCs). By integration of single-cell transcriptomic sequencing data with GWAS (1 million individuals) findings using the summary data-based Mendelian randomization (SMR) method, we identified candidate BP-associated genes across diverse immune cell subsets.

Nanoparticle Drug Delivery Systems for Atherosclerosis: Precision Targeting, Inflammatory Modulation, and Plaque Stabilization.

Atherosclerosis remains a leading contributor to cardiovascular morbidity and mortality, presenting considerable therapeutic challenges due to its multifaceted and complex pathogenesis. This review systematically explores the pathophysiological mechanisms underlying atherosclerosis and examines recent advances in nanoparticle drug delivery systems (NDDSs) tailored for atherosclerosis treatment. NDDS has many advantages in this setting.

A deep-sea hydrothermal vent worm detoxifies arsenic and sulfur by intracellular biomineralization of orpiment (As2S3).

The alvinellid worm Paralvinella hessleri is the only animal that colonizes the hottest part of deep-sea hydrothermal vents in the west pacific. We found P. hessleri accumulates exceptionally high level of toxic element arsenic (>1% of wet weight) and tolerated elevated concentrations hydrogen sulphide.

Interleukin-13 gene polymorphisms and asthma susceptibility in children: a meta-analysis.

Background: Interleukin (IL)-13 is a central mediator of airway inflammation in asthma, and gene polymorphisms may play a role in asthma pathogensis. However, the relationship between IL-13 gene polymorphisms and asthma susceptibility remain unclear. Therefore, we conducted this meta-analysis to evaluate this relationship and better understand the risk of asthma in Chinese children.

Metagenomic analysis reveals how multiple stressors disrupt virus-host interactions in multi-trophic freshwater mesocosms.

Virus-host interactions are vital to microbiome ecology and evolution, yet their responses to environmental stressors under global change remain poorly understood. We perform a 10-month outdoor mesocosm experiment simulating multi-trophic freshwater shallow lake ecosystems. Using a fully factorial design comprising eight treatments with six replicates each, we assess the individual and combined effects of climate warming, nutrient loading, and pesticide loading on DNA viral communities and their interactions with microbial hosts.

Novel PNLDC1 mutations underlie nonobstructive azoospermia in humans and mice.

PIWI-interacting RNAs (piRNAs) are small regulatory RNAs (21-35 nucleotides) exclusively expressed in germ cells, where they play a critical role in transposable element repression and post-meiotic gene regulation. The poly(A)-specific RNase-like domain-containing 1 (PNLDC1) protein is essential for piRNA maturation, specifically in 3'-end trimming. Disruption of PNLDC1 has been implicated in nonobstructive azoospermia (NOA) and male infertility.

Application of machine learning and deep learning in metabolic dysfunction-associated steatotic liver disease: a systematic review and meta-analysis.

Introduction: Metabolic dysfunction-associated steatotic liver disease (MASLD) can progress to metabolic dysfunction-associated steatohepatitis (MASH) and liver fibrosis, contributing to a heavier global health burden. Non-invasive diagnostic tools developed using machine learning (ML) and deep learning (DL), two representative artificial intelligence algorithms, are increasingly being explored for MASH and its related fibrosis assessment.

Objectives: This study aimed to compare the diagnostic performance of different ML and DL models and identify the top-performing models for diagnosing MASH and associated liver fibrosis.

Viral Load Dynamics of Chikungunya Virus in Human Specimens - Foshan City, Guangdong Province, China, 2025.

What Is Already Known About This Topic?: Chikungunya virus (CHIKV) represents an Aedes-borne alphavirus that causes fever, rash, and severe arthralgia. Outbreaks have expanded across 119 countries, with sporadic imported cases documented in China. While serum has been regarded as the optimal diagnostic specimen, comprehensive data comparing multiple specimen types across all infection phases have remained lacking.

Phylogenomic analysis of OXA-23-positive and genetic context of rare NDM-1-producing carbapenem-resistant isolates in a teaching hospital in China.

Nosocomial outbreaks caused by carbapenem-resistant (CRAB) strains are rapidly emerging worldwide and are a cause for concern. In this study, a phylogenetic tree of 18 strains collected from a teaching hospital in China was constructed to explore the genetic relationship in the context of genomic insights. The study also aimed to explore the relationship among the strains and to assess the potential spread within the teaching hospital.

The Class III peroxidase OsPrx20 is a key regulator of stress response and growth in rice.

In plants, reactive oxygen species (ROS) are maintained at strictly low intracellular levels, a prerequisite for their function as signals. Class III peroxidases serve as core regulators of intracellular ROS homeostasis, if environmental stimuli trigger long-term responses causing ROS accumulation. Here, we found a rice Class III peroxidase OsPrx20, which is a target of Ca/calmodulin-dependent protein kinase (OsDMI3) and is phosphorylated at its threonine (Thr)-244 site by OsDMI3, thereby positively regulating osmotic stress tolerance while negatively regulating blast resistance in rice.

Inferior frontal gyrus is responsible for cognitive control during two-digit number processing: fNIRS evidence from magnitude comparison task.

Cognitive control plays an indispensable role in multi-digit number processing. The place-value structure of multi-digit numbers is apparent when comparing numbers. If the comparison of units yields a different result from the comparison of decades, the process takes longer than comparing compatible number pairs.