Insights into heterogenetic mechanism in physicochemical driving factors, microbial community and volatile metabolites during high-temperature Daqu fermentation by traditional stacking and shelf stacking.

Background: Research on shelf-stacking fermentation is growing, with the aim of reducing labor and improving Daqu stability, but it still falls short compared to traditional stacking fermentation, especially for high-temperature Daqu (HTD). The heterogenetic mechanisms underlying+ these two Daqu remain unclear.

Results: The improved micro-oxygen environment in shelf-stacking fermentation significantly reduced acidity and increased liquefaction and esterification activities compared to traditionally stacked HTD (HTD-T).

Non-volatile compounds as aroma modulators in Jiangxiang-flavor Baijiu: Regional flavor differentiation and synergistic interactions with volatile aromas.

A systematic study was undertaken of the flavor disparities of Jiangxiang-flavor Baijiu produced across southern and northern China. Five non-volatile organic acids, seven amino acids, and twenty-one volatile compounds (OAV > 1) were identified, with 14 key aroma-active compounds determined through aroma omission and triangle tests. Quantitative targeted flavoromics and partition coefficient method were undertaken through descriptive sensory analysis.

A magnetic CRISPR/Cas12a-SERS nanobiosensor for amplification-free and ultrasensitive detection of norovirus in water and food samples.

Background: Norovirus (NoV) is the leading cause of foodborne disease outbreaks worldwide, typically spreading via contaminated food and water. Rapid, sensitive, and portable detection of NoV is crucial.

Results: Here, we presented a magnetic CRISPR/Cas12a-SERS nanobiosensor capable of detecting NoV with high sensitivity, accuracy, speed, and portability.

Expanded insights into the mechanisms of RNA-binding protein regulation of circRNA generation and function in cancer biology and therapy.

RNA-binding proteins (RBPs) regulate the generation of circular RNAs (circRNAs) by participating in the reverse splicing of circRNA and thereby influencing circRNA function in cells and diseases, including cancer. Increasing evidence has demonstrated that the circRNA-RBP network plays a complex and multifaceted role in tumor progression. Thus, a better understanding of this network may provide new insights for the discovery of cancer drugs.

Enhanced production of ethyl caproate in strong-flavor Baijiu through a dual bacterial co-culture system and immobilization on natural luffa sponge.

Ethyl caproate, a significant aromatic component in strong-flavor baijiu, is synthesized requiring caproic acid as an essential precursor. In this study, a novel caproic acid-producing bacteria Rummeliibacillus suwonensis J-1 was isolated from pit mud. Subsequently, a dual bacterial co-culture system (DBCS) was successfully established by combining J-1 with the acid-producing bacterium Enterococcus sp.

The family : an emerging microbial resource with high application value.

In recent years, interest has increased in the use of microorganisms to obtain additional valuable resources for green and sustainable development. Preliminary functional analyses have indicated that members of the family have good application potential for the production of novel specific enzymes, high-value bioactive compounds, novel secondary metabolites and the promotion of efficient biomass conversion. Therefore, they can be considered a new class of microbial resources with potentially high value.

Succession of microbial community structure in fermented grains during the fermentation of strong-flavor Baijiu and its impact on the metabolism of acids, alcohols, and esters.

The study clarified the succession of microbial community structures in fermented grains (FG) and their association with flavor compounds, along with their potential metabolic functions. The community diversity, functional genera and metabolites in FG were investigated by polyphasic detecting approaches. There are 13 dominant microorganisms in FG (relative abundance > 1%).

Ferroptosis-related genes DUOX1 and HSD17B11 affect tumor microenvironment and predict overall survival of lung adenocarcinoma patients.

Background: Recent studies have found that ferroptosis-related genes (FRGs) have broad applications in tumor therapy. However, the predictive potential of these genes in lung adenocarcinoma (LUAD) remains to be fully characterized. We aimed to investigate the FRGs that might be potential targets for LUAD.

Establishing a nomogram for predicting necrotizing enterocolitis in premature infants: A retrospective multicenter cohort study.

Background: To construct a nomogram for predicting necrotizing enterocolitis (NEC) in preterm infants.

Methods: A total of 4,724 preterm infants who were admitted into 8 hospitals between April 2019 and September 2020 were initially enrolled this retrospective multicenter cohort study. Finally, 1,092 eligible cases were divided into training set and test set based on a 7:3 ratio.

Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression.

Background: The progression of non-small cell lung cancer (NSCLC) is significantly influenced by circular RNAs (circRNAs), especially in tumor hypoxia microenvironment. However, the precise functions and underlying mechanisms of dysregulated circRNAs in NSCLC remain largely unexplored.

Methods: Differentially expressed circRNAs in NSCLC tissues were identified through high-throughput RNA sequencing.

Improving Muscat Hamburg Wine Quality with Innovative Fermentation Strategies Using Derived from Fermented Grains of Sauce-Flavor Baijiu.

This study investigates innovative approaches to improve the quality and aroma characteristics of Muscat Hamburg wine production by substituting the conventional yeast with an efficient fermentation strain of . The typical use of in Muscat Hamburg wine often leads to uniformity and prolonged processing times, requiring subsequent malolactic fermentation to degrade excessive malic acid. The study advocates for the replacement of with a specific strain, , isolated from the fermented grains of sauce-flavor Baijiu, a Chinese spirit.

Reduced production of Ethyl Carbamate in wine by regulating the accumulation of arginine in Saccharomyces cerevisiae.

Ethyl carbamate (EC), a multisite carcinogenic compound, is naturally produced from urea and ethanol in alcoholic beverages. In order to reduce the content of EC in wine, the accumulation of arginine in Saccharomyces cerevisiae was regulated by genetic modifying genes involved in arginine transport and synthesis pathways to reduce the production of urea. Knockout of genes encoding arginine permease (Can1p) and amino acid permease (Gap1p) on the cell membrane as well as argininosuccinate synthase (Arg1) respectively resulted in a maximum reduction of 66.

Polarization-switchable focal vortex beam by an Archimedean array.

Focal position control of vortex beams has tremendous applications in optical field. Herein, non-classical Archimedean arrays were proposed for optical devices with bifocal length and polarization-switchable focal length. The Archimedean arrays were constructed by rotational elliptical holes in a silver film, which were followed by two one-turned Archimedean trajectories.

Analysis of the Effect of Mixed Fermentation on the Quality of Distilled Jujube Liquor by Gas Chromatography-Ion Mobility Spectrometry and Flavor Sensory Description.

Distilled jujube liquor is an alcoholic beverage made from jujube, which has a unique flavor and a sweet taste. The purpose of this study was to explore the effect of mixed fermentation on the quality of distilled jujube liquor by comparing the performance of mixed fermentation between , and . The results showed that there were significant differences in the quality of the jujube liquor between the combined strains.

Endometriosis and New-Onset Coronary Artery Disease in Taiwan: A Nationwide Population-Based Study.

Endometriosis (EM) with chronic inflammation may accelerate the progression of atherosclerosis. Currently, no large or randomized clinical studies have assessed the incidence of cardiovascular events in patients with endometriosis in Asia to investigate whether incident EM is associated with a higher risk of new-onset coronary artery disease (CAD). In this study of a nationwide cohort in Taiwan, we identified 13,988 patients with newly diagnosed EM from 1 January, 2000, through 31 December, 2012.

Characterization of Microbial Diversity and Community Structure in Fermentation Pit Mud of Different Ages for Production of Strong-Aroma Baijiu.

In the traditional fermentation process of strong-aroma Baijiu, a fermentation pit mud (FPM) provides many genera of microorganisms for fermentation. However, the functional microorganisms that have an important effect on the quality of Baijiu and their changes with the age of fermentation pit (FP) are poorly understood. Herein, the Roche 454 pyrosequencing technique and a phospholipid fatty-acid analysis were employed to reveal the structure and diversity of prokaryotic communities in FPM samples that have been aged for 5, 30, and 100 years.

The efficacy and safety of H1-antihistamine versus Montelukast for allergic rhinitis: A systematic review and meta-analysis.

Purpose: In order to verify the differences of effectiveness and safety between SAHs and Montelukast, and to find out potential uncared-for problems, we performed a systematic review and Meta-analysis to proceed a qualitative describe and quantitative assessment.

Methods: We searched the databases of Pubmed, the Cochrane Library, Nature and Science as well as Wanfang data and CNKI from 2000 to March 2016, using key words "Montelukast SAH" or "H1-antihistamine Montelukast", or "Loratadine Montelukast", or "Desloratadine Montelukast", or "Levocetirizine Montelukast", or "Cetirizen Montelukast", or "Fexofenadine Montelukast". And also we included studies through relevant citations in related literature.

A meta-analysis for the role of proton pump inhibitor therapy in patients with laryngopharyngeal reflux.

We aimed to systematically review the randomized controlled trials (RCTs) regarding the therapeutic efficiency of proton pump inhibitor (PPI) therapy for laryngopharyngeal reflux (LPR). Randomized and placebo-controlled trials regarding the therapeutic efficacy of PPIs on LPR patients were systematically searched from MEDLINE, Cochrane Library, and EMBASE. Data were extracted from eligible studies meeting the inclusion criteria.

Effect of silencing of high mobility group A2 gene on gastric cancer MKN-45 cells.

Aim: To investigate the effect of high mobility group A2 (HMGA2) gene silencing on gastric cancer MKN-45 cells in vitro.

Methods: HMGA2 short hairpin RNA (shRNA) expression plasmids were constructed, including a pair of random scrambled sequences. Human gastric cancer cell line MKN-45 cells were divided into three groups: blank control group (non-transfected cells), transfected group (cells transfected with HMGA2 shRNA recombinant plasmid) and scrambled sequence group (transfected with random scrambled plasmid).