Biomimetic Multilayered Gas-Foaming Scaffold with Sustained bFGF Delivery for Annulus Fibrosus Regeneration.

Current clinical treatments for intervertebral disc (IVD) herniation (e.g., discectomy) often lead to re-herniation, and tissue engineering scaffolds for annulus fibrosus (AF) regeneration remain scarce, particularly those capable of mimicking the multilayered structure of native AF.

Hierarchically Reinforcing Hydrogel for Multidimensional Bone Homeostasis Modulation to Regenerate Osteoporotic Defect.

To balance the requirement of high mechanical properties and convenient injectability for the hydrogel design of osteoporotic bone defects (OBD) repair, a hierarchical hydrogel stiffening strategy is proposed through the synergetic utilizing of rapidly formed dynamic hydrazone crosslinking, high-strength double-bond chemical crosslinking, and contraction of activated shape-memory short fibers (SMSFs). In detail, the Schiff Base reaction mediated first network enabled stable precursor retention at the injection site without compromising injectability. Subsequent radical polymerization by photocrosslinking enhanced the hydrogel stiffness from 9.

Single-cell, spatial and bulk transcriptome data analysis revealed LINC00467-mediated Sertoli cell ferroptosis is a potential therapeutic target and biomarker for azoospermia.

Human spermatogenesis is an important physiological process related to programmed cell death. However, which type of programmed cell death playing a key role in normal and abnormal human spermatogenesis remains obscure. This study integrated single-cell, bulk RNA and spatial transcriptome data analysis and found that the ferroptosis signal plays a potential role in spermatogenesis and significantly elevate in testicular samples from humans with non-obstructive azoospermia (NOA) due to various factors.

Navigating Challenges and Building Resilience: Qualitative Insights into Disaster Nursing Preparedness in Chinese Non-First-Tier Cities.

Objectives: This qualitative study aimed to explore the lived experiences, challenges, and perceptions of nurses regarding disaster preparedness in non-first-tier (inland) cities in China. Building upon our previous quantitative findings, we sought to understand the psychological, professional, and organizational factors that influence nurses' disaster response capabilities in resource-limited settings.

Methods: A descriptive phenomenological approach was employed.

Identification of Cembrane Diterpenoids from sp. That Reduce the Viability of Diffuse Pleural Mesothelioma Cell Lines.

Diffuse pleural mesothelioma (DPM) is a rare but aggressive late-onset cancer. A high-throughput screen of a natural product fraction library identified fractions derived from an aqueous extract of sp. soft coral that reduced the viability of DPM cell lines.

Efficacy and safety of transcranial direct current stimulation for children and adolescents with attention-deficit/hyperactivity disorder: a systematic review and meta-analysis.

Background: Children and adolescents with attention-deficit/hyperactivity disorder (ADHD) often show cognitive deficits. Given that some evidence has suggested transcranial direct current stimulation (tDCS) as a potential alternative or adjunct to psychostimulants, we sought to perform a meta-analysis and systematic review to evaluate the effects of tDCS on clinical symptoms and cognitive function among children and adolescents with ADHD, as well as to summarize associated adverse effects.

Methods: We searched PubMed, Embase, Web of Science, Scopus, and the Cochrane Library up to May 7, 2025 for randomized controlled trials (RCTs) involving children and adolescents with ADHD who underwent tDCs therapy.

Stopping Tuberculosis at the Gate: The Role of Adhesins in Infection and Intervention.

The global burden of tuberculosis (TB), exacerbated by the rise of drug-resistant (), underscores the need for alternative intervention strategies. One promising approach is to block the infection at its earliest stage-bacterial adhesion to host cells-thereby preventing colonization and transmission without exerting selective pressure. Adhesins, surface-exposed molecules mediating this critical interaction, have therefore emerged as attractive targets for early prevention.

Effects of ambient particulate matter pollution on daily hip fracture admissions in middle-aged and older adults: a time series study in Shantou, China.

Background: Evidence on the effects of particulate matter (PM) on hip fractures is lacking, especially in developing countries. The purpose of this study was to examine the association of particulate matter with hip fracture admissions in middle-aged and elderly people in the Chinese coastal city of Shantou.

Methods: Daily hip fracture admission, meteorological factor and air pollution data were collected from 2015 to 2019.

Bone marrow-derived myeloid cells drive neuroinflammation in Alzheimer's disease: Insights from the FAD mouse model.

Objective: Alzheimer's disease (AD) is marked by amyloid β (Aβ) accumulation, neuroinflammation, and cognitive decline. While neuroinflammation is a key feature of AD, the potential involvement of bone marrow-derived cells in its pathology remains unclear. This study aimed to investigate the role of bone marrow-derived myeloid cells in driving neuroinflammation in AD.

The Discovery of Complex Heterocycles from Millipede Secretions.

The recent explosion in documented chemistry from Colobognatha, a millipede subterclass with more than 240 species, has rekindled interest in the defensive secretions from these ancient animals. Prior to 2020, studies on defensive secretions by Colobognatha─the only millipedes that produce terpenoid alkaloids─were limited to a single order, Polyzoniida. However, numerous species of the order Platydesmida have recently been shown to produce structurally diverse terpenoid alkaloids with potent biological activities.

Liquid-liquid interfacial tension stabilized Li-metal batteries.

A lithium (Li)-metal anode paired with a high-nickel cathode is considered to be a combination that holds promise to surpass the 500 Wh kg threshold. Approaching such high energy density, electrolytes capable of stabilizing both anode and cathode interphases are of importance to secure safe and long-term cycling. Although anion-derived inorganic interphases have shown remarkable success at the Li side, developing intrinsic strategies to concurrently protect both electrodes remains a key challenge.

CircRNA Sequencing Analysis Reveals the Regulatory Role of circ_CDR1as in Penile Squamous Cell Carcinoma via the ceRNA Network.

Penile squamous cell carcinoma (PSCC) is a malignant reproductive tumor, and circRNAs are essential regulators in the progression of cancers. However, their specific roles in PSCC have not been adequately investigated, which are available to function via the competing endogenous RNA (ceRNA) network. We collected 5 normal and 6 PSCC tissue samples for bulk RNA sequencing and obtained differentially expressed circRNAs (DE-circRNAs) and differentially expressed mRNAs (DE-mRNAs).

Identification and analysis of cuproptosis associated molecular clusters and immunological profiles in atopic dermatitis.

Background: Atopic dermatitis (AD) is a chronic skin condition marked by persistent itching and dryness. The role of cuproptosis, a novel form of programmed cell death, in AD is not yet understood.

Methods: The GSE107361 dataset was obtained from the Gene Expression Omnibus (GEO) database.

Injectable scaffolds with a hierarchically porous structure and augmented paracrine activity for minimally invasive precision medicine.

Stem cell-based therapeutic approaches hold great potential for regenerating severe tissue defects, however, current cell transplantation strategies cannot simultaneously achieve minimally invasive injection and structural integrity. Herein, an injectable 3D-bioprinted scaffold encapsulated with two-phase emulsion bioink-engineered mesenchymal stromal cells is proposed, which can serve as a novel versatile platform for efficient stem cell delivery in minimally invasive approaches. The two-phase emulsion bioinks could spontaneously undergo a phase separation process in an aqueous environment to form hierarchically porous structures (macropore size of 1 mm and micropore size of 100-200 µm), which not only facilitated the proliferation and spreading of the encapsulated cells but also endowed the bioprinted scaffolds with shape memory properties for injection.

Antiproliferative cassaine diterpenoid amines from the leaves and twigs of the traditional Thai medicinal plant Erythrophleum teysmannii (Kurz) Craib.

Ethnopharmacological Relevance: Erythrophleum teysmannii (Kurz) Craib is a medicinal plant used by traditional healers in Laos and Thailand to treat cancer. Despite ethnopharmacological reports about plant extracts from this genus as cancer drug formulae, there is a knowledge gap about the chemical composition and potential pharmacological effects of E. teysmannii.

γ-secretase targeting in Alzheimer's disease.

Alzheimer's disease (AD) is one of the most prevalent neurodegenerative disorders and is characterized by memory loss and cognitive decline. The amyloid cascade hypothesis posits that the pathogenesis of AD is initiated by the oligomerization and accumulation of toxic amyloid-β (Aβ) peptides within the brain. The aspartic protease γ-secretase, which catalyzes the final step in the cellular production of Aβ peptides, has been identified as a potential target for anti-amyloid intervention strategies.

Chemical Evolution of Aplithianine Class of Serine/Threonine Kinase Inhibitors.

Chimeric kinase J-PKAcα represents a potential therapeutic target for fibrolamellar hepatocellular carcinoma (FLHCC). Structure-based design and screening were applied to improve the potency of the marine-derived kinase inhibitor aplithianine A targeting J-PKAcα. Three classes of aplithianines (I, II, and III) including >150 analogs were synthesized, significantly improving biochemical IC values to the low nanomolar range.

Machine learning-based prediction of volatile compounds profiles in Saccharomyces cerevisiae fermentation simulating canned meat.

Aroma and precision fermentation converge in exciting ways, enabling the precise production of aromatic compounds. Precision fermentation employs engineered microorganisms to create and refine scents and aromas with high accuracy, allowing for customizable aromas and opening new possibilities for both culinary experiences and consumer products. Structured data on volatile compounds from canned meat and fermented products was compiled to train machine learning (ML) models aimed at predicting volatile compounds and simulating meat aroma in Saccharomyces cerevisiae.

Furanoheliangolides from and a General Approach for Stereochemical Assignment of Flexible Chiral Side Chains.

Human topoisomerase 3β (TOP3B) is a potential molecular therapeutic target for cancer and viral infections. A high-throughput differential cell viability assay using colon cancer cell lines was developed to identify natural product modulators of TOP3B-associated cancer cell viability. The assay identified an organic extract of the plant as having cytotoxic activity.

Abnormalities of brain dynamics based on large-scale cortical network modeling in autism spectrum disorder.

Synaptic increase is a common phenomenon in the brain of autism spectrum disorder (ASD). However, the impact of increased synapses on the neurophysiological activity of ASD remains unclear. To address this, we propose a large-scale cortical network model based on empirical structural connectivity data using the Wendling model, which successfully simulates both pathological and physiological electroencephalography (EEG) signals.

Comparing interpretable machine learning models for fall risk in middle-aged and older adults with and without pain.

Pain is common in middle-aged and older adults, has also been identified as a fall risk factor, whereas the mechanism of falls in pain is unclear. This study included 13,074 middle-aged and older adults from the China health and retirement longitudinal study (wave 2011-2015) to separately develop four-year fall risk prediction models for older adults with and without pain, using five machine learning algorithms with 145 input variables as candidate features. Shapley Additive exPlanations (SHAP) was used for the prediction model explanations.

Sirtuin 1 underlies depression-related behaviors by modulating the serotonin system in the dorsal raphe nucleus in female mice.

Major depressive disorder (MDD) is a primary driver of disability and greatly escalates the worldwide disease burden. Sirtuin 1 (Sirt1), a key regulator of cellular metabolism, is associated with genetic variations in MDD. We investigated how Sirt1 in serotonin (5-HT) neurons within the dorsal raphe nucleus (DRN) in mice affected behaviors associated with depression and susceptibility to stress.

Sesquiterpene Coumarin Ethers and Phenylpropanoids from the Roots of , the Putative Anatolian Ecotype of the Silphion Plant.

Four new sesquiterpene coumarin ethers (-) and a new phenylpropanoid compound () were isolated from a hexane extract of the roots of , the putative Anatolian ecotype of the silphion plant, in addition to nineteen previously described sesquiterpene coumarins (-) and four known phenylpropanoid derivatives (-). The structures of these compounds were elucidated by extensive spectroscopic analysis and computational studies. The cytotoxic activities of all isolated compounds were evaluated on renal, malignant pleural mesothelioma (MPM) and colon cancer cell lines.