Alternative Splicing of the NMDA Receptor Subunit GluN1 Mediated by Polypyrimidine Tract-Binding Protein Dimerization in the Trigeminal Ganglion Contributes to Orofacial Allodynia.

Orofacial neuropathic pain, a debilitating condition associated with trigeminal nerve injury, is often characterized by allodynia. N-methyl-d-aspartate receptors (NMDARs), particularly the GluN1 subunit, play a central role in mediating this pain. The GluN1 subunit undergoes alternative splicing at exon 5, generating isoforms GluN1a (lacking the exon 5-encoded N1 cassette) and GluN1b (retaining the N1 cassette), which have distinct functional roles.

Effects of a 6-week semi-supervised exercise training program on physical fitness and mental health in post-COVID-19 healthcare workers: a randomized clinical trial.

Background: Healthcare workers rank among the groups that are most vulnerable to the SARS-CoV-2 infection. Deterioration in physical fitness and mental health is a common manifestation in the post-COVID-19 person, which require specific and effective treatment. This study aimed to evaluate the effects of a 6-week, semi-supervised exercise intervention on physical fitness and mental health in post-COVID-19 healthcare workers (HCWs).

Dual-modal fusion of hierarchical image features and spectral data for efficient quantitative analysis of mineral elements in rice (Oryza sativa L.) leaves.

Rapid and accurate quantification of mineral elements in plants facilitates the optimization of cultivation strategies and provides theoretical support for heavy metal pollution control. Compared to traditional chemical detection methods, laser-induced breakdown spectroscopy (LIBS) offers rapid, simultaneous multi-element analysis. However, the quantitative accuracy of LIBS is often hindered by challenges such as sample heterogeneity and the inherent matrix effects arising from the physical and chemical properties of samples.

Erratum: [Corrigendum] LSKL peptide alleviates subarachnoid fibrosis and hydrocephalus by inhibiting TSP1-mediated TGF-β1 signaling activity following subarachnoid hemorrhage in rats.

[This corrects the article DOI: 10.3892/etm.2016.

[Clinical efficacy of in-situ crown reattachment technique combined with pulpotomy in the treatment of complica-ted crown-root fractures of young permanent anterior teeth].

Objectives: This study aimed to evaluate the clinical application value of in-situ crown reattachment technique combined with pulpotomy in the treatment of complicated crown-root fractures in young permanent anterior teeth.

Methods: A prospective study was conducted on 50 children with complicated crown-root fractures in young permanent anterior teeth, who were treated at the Pediatric Dentistry Department, Hospital of Stomatology, Xi'an Jiaotong University from June 2023 to June 2024. All patients underwent in-situ crown reattachment technique combined with pulpotomy.

Occurrence, maternal transfer, and risk implications of parabens in humpback dolphins from the South China Sea.

Parabens (PBs), extensively used as preservatives in food, cosmetics, and pharmaceuticals, are increasingly detected across diverse environmental matrices. Nevertheless, their presence and potential impacts on marine apex predators remain poorly understood. Here, hepatic tissues from 87 Indo-Pacific humpback dolphins collected from the northern South China Sea (NSCS) during 2004-2021 were analyzed for four PBs and five paraben metabolites (m-PBs) to investigate their bioaccumulation characters and potential health risks.

Clinical safety study of laparoscopic common bile duct exploration and primary suture in elderly patients: a new strategy for the treatment of stones.

Background: Laparoscopic common bile duct exploration and primary suture (LBEPS) offers advantages such as minimal invasion, shorter operative time, and faster postoperative recovery in the treatment of common bile duct stones.However, its safety in elderly patients remains controversial.

Methods: A retrospective analysis was conducted on the clinical data of 128 patients with common bile duct stones who underwent LBEPS treatment in our hospital from December 2018 to March 2025.

Records of human activities in sediments from the old Yellow River estuary, China: Concentrations, sources, and ecological risks of organochlorine pesticides.

Organochlorine pesticides (OCPs) are important indicators for assessing the intensity of human activities, while estuarine sediments are widely used to reconstruct the historical deposition of pollutants. In this study, a sediment core was collected from the old Yellow River estuary in the Yellow River Delta, China, to analyze the historical deposition of OCPs in detail. The results indicate that the sediment record extends back to 1941, with an average sedimentation rate of 1.

2.5D Deep Learning and Machine Learning for Discriminative DLBCL and IDC with Radiomics on PET/CT.

We aimed to establish non-invasive diagnostic models comparable to pathology testing and explore reliable digital imaging biomarkers to classify diffuse large B-cell lymphoma (DLBCL) and invasive ductal carcinoma (IDC). Our study enrolled 386 breast nodules from 279 patients with DLBCL and IDC, which were pathologically confirmed and underwent F-fluorodeoxyglucose (F-FDG) positron emission tomography/computed tomography (PET/CT) examination. Patients from two centers were separated into internal and external cohorts.

Winding fault detection based on current information of induction motors.

As an information carrier, current signal directly reflects the electromechanical coupling between the stator and rotor of an induction motor. In this study, a detection method of winding fault based on current information is proposed to identify fault and realize feature decoupling for an induction motor. Unlike most current analysis methods, this method combines the electromagnetic field distribution with the feature frequency of the current signal to achieve the detection of the stator winding fault.

Regulatory mechanisms and therapeutic potential of natural microglia inhibitors in Parkinson's disease.

Parkinson's disease (PD) is one of the most prevalent neurodegenerative diseases affecting the central nervous system. The pathogenesis of PD is complex, and its etiology remains poorly understood. Recently, it has been suggested that neuroinflammation caused by overactivated microglia plays a significant role in the occurrence and development of PD.

Hydrodynamically Controlled Abiotic Recycling of Organic Phosphorus in Riparian Sediments.

Organic phosphorus (OP), a major phosphorus pool in sediments, can be converted into bioavailable phosphate. Although biological activity is considered the primary driver of OP dephosphorylation, the abiotic mechanism under redox oscillations remains unexplained. Here, we show that hydrological perturbation-driven redox fluctuations can mediate abiotic phosphate release from OP.

Whole exome sequencing analysis of susceptibility loci in transgender individuals.

Background: Genetic factors contributing to sex-associated dimorphic brain development may also underlie gender identity-related anxiety disorders.

Aim: To establish a high-throughput whole-exome sequencing (WES) and bioinformatics pipeline for identifying rare variants in sex-dimorphic neural pathways and explore their association with gender identity-related anxiety.

Methods: Peripheral genomic DNA was collected from 23 patients (13 Assigned male at birth (AMAB), 10 Assigned female at birth (AFAB)) presenting with gender identity-related anxiety at Shanghai Mental Health Centre between March 2020 and February 2022.

Opportunities, obstacles and challenges of nano-immunotherapy in melanoma.

Melanoma is an exceptionally aggressive form of skin cancer, and its prognosis becomes dire once it metastasizes. Although substantial progress has been made in the field of immunotherapy, significant hurdles such as tumor cell immune evasion, the tumor microenvironment (TME), and immune-related adverse effects persist. Recent advancements in nanotechnology offer promising solutions to these challenges by enhancing targeting, stability, and delivery of immunotherapeutic agents.

Facet-Engineered (100)-Oriented MoO Nanoribbons for Broadband Self-Powered Photodetection.

Broadband photodetection plays a vital role in aerospace applications, biomedical imaging, and advanced communication systems. While molybdenum dioxide (MoO) exhibits exceptional electrical conductivity, carrier mobility, and environmental stability, its potential for photodetection has remained unrealized, with existing literature reporting negligible optoelectronic responses. Here, we unlock latent photoresponsivity of MoO by facet engineering, demonstrating that exposing the (100) crystallographic plane activates its intrinsic photoelectric conversion.

A Stepwise Management Strategy for Treatment of Iatrogenic Upper Eyelid Retraction in Eastern Asian Eyelids.

Background: Incidence of iatrogenic upper eyelid retraction (UER) caused by blepharoptosis overcorrection and improper blepharoplasty is increasing fast among Eastern Asians. The aim of this study is to present our experience using techniques of pretarsal cicatrix release, recession of levator-Müller's muscle complex, and lengthening of levator-Müller's muscle complex to correct mild to severe UER, and evaluate their anesthetic surgical outcomes.

Methods: Patients with UER who underwent surgical repair using the stepwise management strategy from December 2018 to June 2022 were retrospectively reviewed.

Development of Thymic Organoids Heterotopically to Educate and Induce T Lymphocytes.

Background: Vascularized composite allotransplantation (VCA) is a potential treatment for extensive injuries that replaces defects like-with-like, however allografts are immune-rejectable.

Methods: This study developed in vitro thymic organoids and examined whether donor-derived HSCs could be educated in vivo into T lymphocytes via central tolerance. TECs, TMCs, and HSCs from C57BL/7 (CD45.

Efficient Synthesis of Clovibactin and Its Analogues: Promoting the Discovery of More Potent Antibiotics via Multiple Screenings.

Increasingly, antimicrobial resistance has become a major concern for public health. The recently discovered cyclic depsipeptide, clovibactin, is a potential antibiotic candidate with potent activities against multidrug-resistant pathogens. It contains three d-amino acids, including a rare d-hydroxyasparagine residue.

Bond strength enhancement for additively manufactured zirconia: Micro-retentive design approach.

Purpose: To investigate the effect of digitally designed micro-retention features on the bond strength of additively manufactured (AM) zirconia copings.

Materials And Methods: Sixty titanium base abutments (NobelParallel, NobelBiocare), pretreated with 50 µm alumina particles were divided into four groups (n = 15 per group); one group with subtractively manufactured zirconia copings (SM), and three test groups comprising one group with AM zirconia copings without any intaglio surface modification (AM-N), and two groups with digitally designed inverted pyramid-shaped micro-textures incorporated to the intaglio surface of the AM zirconia copings with two sizes: Ra 120 µm (AM-120) and Ra 240 µm (AM-240). All copings were bonded to the abutments using a conventional resin cement (Multilink Hybrid).

Efficacy and Safety of HSK31679 in Asian Patients with MASLD: A Randomized Controlled Trial.

Background And Aims: This study evaluated the efficacy and safety of the thyroid hormone receptor β agonist HSK31679 in Asian patients with metabolic dysfunction-associated steatotic liver disease (MASLD).

Methods: This was a phase 2a, multicenter, randomized, double-blind, placebo-controlled study at 26 centers in China. Subjects with a liver fat content (LFC) of ≥ 8% at screening, when assessed by magnetic resonance imaging-proton density fat fraction, were eligible.

Predicting early hematoma enlargement in spontaneous intracerebral hemorrhage: Hypocalcemia, Hyperglycemia, and CT signs.

Background: Spontaneous intracerebral hemorrhage (sICH) is a severe, stroke subtype, and early hematoma enlargement (HE) strongly predicts mortality and disability. This study aimed to investigate the predictive value of hypocalcemia, hyperglycemia, and computed tomography (CT) imaging signs for early HE in sICH.

Methods: A total of 238 sICH patients admitted to the First People's Hospital Affiliated to Shanghai Jiao Tong University from November 2021 to November 2024 were retrospectively included.

Identification of Mild Hepatic Encephalopathy Based on Multi-level Functional Connectivity Hypernetwork.

Early diagnosis of mild hepatic encephalopathy is important for the reversion of hepatic encephalopathy. Brain hyper-connectivity networks with hyperedges have showed good performance for diagnosis of neurological disorders. However, the previous hyper-connectivity networks is essentially low-level since the temporal synchronization of regional signal fluctuation is merely considered.

Research trends and topics on sepsis immunosuppression: a bibliometric and visual analysis of global research from 2004 to 2024.

Background: Sepsis, a condition characterized by critical organ impairment resulting from dysregulated response to infection, remains a leading cause of mortality globally. Immunosuppression significantly dictates the clinical trajectory and ultimate prognosis in sepsis. Research on sepsis-related immunosuppression has gained substantial attention in recent years.

Effects of single and co-cultured lactic acid bacteria on antioxidant capacity and metabolite profiles during rambutan juice fermentation.

As a tropical fruit, rambutan has been predominantly studied for its peel. To explore the application of lactic acid bacteria (LAB) in rambutan juice fermentation, we investigated the metabolic characteristics of juice fermented with and . This study reveals the distinct metabolic characteristics and synergistic mechanisms of and during rambutan juice fermentation.

DDX42 Enhances Hepatocellular Carcinoma Cell Proliferation, Radiation and Sorafenib Resistance via Regulating GRB2 RNA Maturation and Activating PI3K/AKT Pathway.

The DEAD-box RNA helicase (DDX) family is one of the canonical splicing regulators, engaged in RNA metabolism, and generally participates in forming spliceosomes. However, systematic analysis of DDX family members in hepatocellular carcinoma (HCC) has not been conducted before, and their biological functions need to be investigated further. Based on biological function enrichment analysis, radiosensitivity index (RSI), and prediction IC50 index for sorafenib, we ultimately ascertain DDX42 as a candidate gene.