Alternative Splicing of the NMDA Receptor Subunit GluN1 Mediated by Polypyrimidine Tract-Binding Protein Dimerization in the Trigeminal Ganglion Contributes to Orofacial Allodynia.

Orofacial neuropathic pain, a debilitating condition associated with trigeminal nerve injury, is often characterized by allodynia. N-methyl-d-aspartate receptors (NMDARs), particularly the GluN1 subunit, play a central role in mediating this pain. The GluN1 subunit undergoes alternative splicing at exon 5, generating isoforms GluN1a (lacking the exon 5-encoded N1 cassette) and GluN1b (retaining the N1 cassette), which have distinct functional roles.

[Improvement effect of rehabilitation nursing based on IKAP theory on patients with urinary incontinence after radical prostatectomy].

Objective: To explore the improvement effect of rehabilitation nursing based on information-knowledge-belief-behavior (IKAP) theory on urinary incontinence patients after radical prostatectomy.

Methods: Sixty-six patients with urinary incontinence who received robot-assisted laparoscopic radical prostatectomy in General Hospital of Eastern Theater Command from January 2021 to January 2023 were selected and divided into control group (n=33) and observation group (n=33) according to random number table method. The patients in the control group were treated with rehabilitation nursing.

Comparison of the Rockall Score and Revised Rockall Score for predicting outcomes in nonvariceal upper gastrointestinal bleeding.

Background: The Rockall score (RS) is used to assess the prognosis of patients with nonvariceal upper gastrointestinal bleeding. Aims: To establish the Revised Rockall Score (RRS) that incorporates an assessment of endoscopic treatment results, and analyze the discriminative ability of the RRS for 30-day rebleeding and mortality in patients with nonvariceal upper gastrointestinal bleeding.

Methods: We retrospectively analyzed data from 432 patients between January 2016 and December 2019 (derivation cohort), and further evaluated in an independent population of 290 patients between January 2020 and December 2022 (validation cohort).

Risk Factors Predicting Positive Surgical Margins Following Conization and Residual Disease in Subsequent Hysterectomy Among Postmenopausal Women With Cervical Intraepithelial Neoplasia.

Aim: To assess the risk factors for positive margin and residual high-grade lesions after cold knife conization (CKC) in postmenopausal patients.

Methods: This retrospective study included a total of 173 postmenopausal patients aged ≥50 years who underwent hysterectomy after CKC at Peking University Third Hospital between September 2012 and February 2023. Statistical analyses were carried out using SPSS 22.

NR2A-Wnt-TLR2 signaling axis mediates neuronal mitochondrial dysfunction contributing to neuropathic pain induced by nerve injury in diabetic mice.

Diabetic neuropathy, a prevalent complication of diabetes mellitus (DM), induces neuropathic pain (NP) through spinal dorsal horn (SDH) central sensitization, with mitochondrial dysfunction emerging as a pivotal contributor. While N-methyl-d-aspartate receptor (NMDAR) subunit NR2A and Toll-like receptor 2 (TLR2) are implicated in NP pathogenesis, their mechanistic interplay in DM-associated mitochondrial dysregulation remains undefined. Here, we systematically investigated their involvement in central sensitization using gain-of-function and loss-of-function strategies for NR2A and TLR2.

Engineered RVG-exosomes-mediated delivery of siRNAs targeting NMDAR alleviates orofacial neuropathic pain by suppressing central sensitivity.

Orofacial neuropathic pain (NP) is a common complication in oral clinical practice that severely affects quality of life. However, NP does not currently receive effective medications. Engineered exosomes have great potential as drug delivery systems for treating diseases.

The Double-Edged Effect of Connexins and Pannexins of Glial Cells in Central and Peripheral Nervous System After Nerve Injury.

Glial cells play pivotal roles in homeostatic regulation and driving reactive pathologic changes after nerve injury. Connexins (Cxs) and pannexins (Panxs) have emerged as seminal proteins implicated in cell-cell communication, exerting a profound impact on the response processes of glial cell activation, proliferation, protein synthesis and secretion, as well as apoptosis following nerve injury. These influences are mediated through various forms, including protein monomers, hemichannel (HC), and gap junction (GJ), mainly by regulating intercellular or intracellular signaling pathways.

Low Circulating Levels of Omentin-1 and Irisin in Type 2 Diabetes Mellitus Patients with Metabolic-Associated Fatty Liver Disease.

Background/aims: The study analyzed the roles of circulating omentin-1 and irisin in patients with type 2 diabetes mellitus (T2DM) concomitant with metabolic-associated fatty liver disease (MAFLD).

Materials And Methods: This cross-sectional study included 80 patients with T2DM but no MAFLD, 62 patients with MAFLD but no T2DM, 50 T2DM patients having MAFLD (T2DM/MAFLD), and 80 healthy individuals.

Results: The serum levels of omentin-1 and irisin were both significantly reduced in patients with T2DM coexisting with MAFLD compared to T2DM or MAFLD patients alone.

Association between coronary heart disease and risk of advanced liver fibrosis: results of the 2017-2018 national health and nutrition examination survey.

Background: Studies have shown that advanced liver fibrosis is strongly associated with cardiovascular risk factors (including age and smoking status). Nevertheless, the exact mechanism is not yet clear. Hence, this study aimed to investigate the relationship between coronary heart disease (CHD) and advanced liver fibrosis and to reveal the developmental processes.

Loss of SCRG1 in chondrocytes inhibits osteoarthritis by promoting autophagy activity in the temporomandibular joint through inhibition of neurokine receptors.

Background: To investigate how scrapie responsive gene 1 (SCRG1) contributes to the development of temporomandibular joint osteoarthritis (TMJOA).

Methods: Western blotting was used to identify protein expression. Proinflammatory cytokine levels were assessed by means of an enzyme-linked immunosorbent test.

[Research Progress on Plastic Aging Processes and Their Environmental Hazards].

Plastic pollution has become a global environmental issue, posing notable threats to ecosystems and human health. Plastic aging is a continuous dynamic process, during which plastics of various sizes are produced and exist, such as large plastics, microplastics, and nanoplastics. Regardless of the size, plastics continuously fragment and release plastic additives and other soluble products.

Drug-drug interaction study with itraconazole supplemented with physiologically based pharmacokinetic modelling to characterize the effect of CYP3A inhibitors on venglustat pharmacokinetics.

Aims: Venglustat is an oral glucosylceramide synthase inhibitor under clinical investigation to treat various lysosomal storage diseases. Metabolism is a main pathway for its elimination in humans with CYP3A being the major contributor. This study aims to evaluate effect of CYP3A inhibition (using itraconazole) on venglustat exposure and to develop and validate a physiologically based pharmacokinetic (PBPK) model to assess effects of additional CYP3A inhibitors of varying potencies on venglustat pharmacokinetics.

Activation of NR2A-Wnt-TLR2 Signaling Axis in Satellite Glial Cells of the Dorsal Root Ganglion Contributes to Neuropathic Pain Induced by Nerve Injury in Diabetic Mice.

Diabetic peripheral neuropathic pain (DPNP), a common diabetic mellitus (DM) complication, may result from the activation of satellite glial cells (SGCs) in the dorsal root ganglion (DRG), potentially enhancing peripheral sensitization. The N-methyl-D-aspartate receptor (NMDAR) subtype NR2A and Toll-like receptor (TLR)2 play key roles in neuroimmune interactions. However, their roles in SGCs of DRG and the precise mechanisms mediating peripheral sensitization in DPNP remain unclear.

The amplitude of low frequency fluctuation and spontaneous brain activity alterations in age-related macular degeneration.

Background: Wet age-related macular degeneration (wAMD) is a vision-threatening eye disease worldwide. The amplitude of low-frequency fluctuation (ALFF) method was used to observe changes in spontaneous brain activity, which may help to investigate the underlying pathological mechanism of AMD.

Methods: Eighteen patients with wAMD and 18 age- and gender-matched healthy controls (HCs) were recruited.

Abnormal widening of the mandibular canal -A characteristic and valuable imaging phenomenon.

Abnormal widening of the mandibular canal (MC) is rarely observed on radiography. Nonetheless, as most of the current research on abnormal mandibular widening is limited to case reports and MCs have relatively deep and hidden positions, there are challenges in diagnosing and formulating treatment plans for patients with abnormal MC widening. To provide ideas for the differential diagnosis and treatment choices of the characteristic clinical sign, this study included patients with abnormal widening of the MC between July 2014 and October 2023.

The crucial role of NR2A mediating the activation of satellite glial cells in the trigeminal ganglion contributes to orofacial inflammatory pain during TMJ inflammation.

Temporomandibular joint inflammatory diseases are a significant subtype of temporomandibular disorders (TMD) characterized by inflammatory pain in the orofacial area. The N-methyl-D-aspartate receptor (NMDAR), specifically the NR2A subtype, was crucial in neuropathic pain. However, the exact role of NR2A in inflammatory pain in the TMJ and the molecular and cellular mechanisms mediating peripheral sensitization in the trigeminal ganglion (TG) remain unclear.

Correction: polysaccharides ameliorate lipid metabolic disorders and gut microbiota dysbiosis in high-fat diet fed rats.

Correction for ' polysaccharides ameliorate lipid metabolic disorders and gut microbiota dysbiosis in high-fat diet fed rats' by Lu Li , , 2019, , 2560-2572, https://doi.org/10.1039/C9FO00075E.

Jaw relation recording and transferring: Using digital technology to augment its instruction in predoctoral prosthodontics.

Objectives: The study compared the learning effectiveness and teaching satisfaction of dental students from three teaching methods (traditional, digital, and combined pedagogy) for instructing jaw relation recording and transferring (JRRT).

Methods: Note that, 276 fourth-year undergraduate students from three classes were randomly divided into three groups to study JRRT in complete dentures using different teaching methods: traditional pedagogy group, digital pedagogy group, and combined digital and traditional pedagogy group. After the course, the learning effectiveness of basic knowledge and satisfaction questionnaire was administered to the three groups of participants using a quick response code for online answering.

Bioinformatics and experimental validation were combined to explore lactylation-related biomarkers in HBV-associated acute liver failure.

Background And Aim: Currently, hepatitis B virus-related acute liver failure (HBV-ALF) has limited treatment options. Studies have shown that histone lactylation plays a role in the progression of liver-related diseases. Therefore, it is essential to explore lactylation-related gene (LRGs) biomarkers in HBV-ALF to provide new information for the treatment of HBV-ALF.

Metal Ion Modulated Electron Transfer in Metalloviologen Compounds: Photochromism and Differentiable Amine Detection.

Different types of electron transfers (ETs) underlie the versatile use of various solid viologen-derived compounds, which is still insufficiently understood and difficult to control. Here, we demonstrate an effective strategy for modulating the key ET process in crystalline metalloviologen compounds (MVCs). By adjusting the coordinated transition metal ions bearing different electronic structures (e.

Contribution of inwardly rectifying potassium channel 4.1 in orofacial neuropathic pain: Regulation of pannexin 3 via the reactive oxygen species-activated P38 MAPK signal pathway.

The involvement of inwardly rectifying potassium channel 4.1 (Kir4.1) in neuropathic pain has been established.

N-methyl-D-aspartate Receptor Subunits 2A and 2B Mediate Connexins and Pannexins in the Trigeminal Ganglion Involved in Orofacial Inflammatory Allodynia during Temporomandibular Joint Inflammation.

Temporomandibular joint osteoarthritis (TMJOA) is a severe form of temporomandibular joint disorders (TMD), and orofacial inflammatory allodynia is one of its common symptoms which lacks effective treatment. N-methyl-D-aspartate receptor (NMDAR), particularly its subtypes GluN2A and GluN2B, along with gap junctions (GJs), are key players in the mediation of inflammatory pain. However, the precise regulatory mechanisms of GluN2A, GluN2B, and GJs in orofacial inflammatory allodynia during TMJ inflammation still remain unclear.