Alternative Splicing of the NMDA Receptor Subunit GluN1 Mediated by Polypyrimidine Tract-Binding Protein Dimerization in the Trigeminal Ganglion Contributes to Orofacial Allodynia.

Orofacial neuropathic pain, a debilitating condition associated with trigeminal nerve injury, is often characterized by allodynia. N-methyl-d-aspartate receptors (NMDARs), particularly the GluN1 subunit, play a central role in mediating this pain. The GluN1 subunit undergoes alternative splicing at exon 5, generating isoforms GluN1a (lacking the exon 5-encoded N1 cassette) and GluN1b (retaining the N1 cassette), which have distinct functional roles.

An E120R Gene-Deleted African Swine Fever Virus Is Fully Attenuated and Induces Partial Protective Immunity in Pigs.

African swine fever virus (ASFV) causes a lethal hemorrhagic disease in domestic pigs and represents a major threat to the global swine industry. Until now, no effective commercial vaccines or antiviral drugs are available for ASF control. In this study, we constructed a recombinant E120R gene-deleted virus, ASFV-ΔE120R, based on the highly virulent genotype II strain SY18, to investigate the biological role of the E120R gene.

Buyang huanwu decoction protects against cerebral ischemia-reperfusion injury by inhibiting complement and coagulation cascades: A proteomics and experimental study.

Objective: Proteomics Approach to Investigate the Mechanism of Buyang Huanwu Decoction(BYHWD) in Ameliorating Cerebral Ischemia-Reperfusion Injury through Inhibition of Complement and Coagulation Cascade Activation.

Methods: A Rat Model of Qi Deficiency and Blood Stasis Induced Cerebral Ischemia-Reperfusion Injury Was Established Using Exhaustive Swimming Combined with Middle Cerebral Artery Occlusion Model(MCAO). The therapeutic effects were evaluated through neurological scoring, TTC staining, and HE staining.

Genomic Detection and Delineation of Chromoanasynthesis by Mate-Pair Sequencing.

Chromoanagenesis encompasses catastrophic genomic rearrangements, with chromoanasynthesis referring to unbalanced germline events involving one or multiple chromosomes, distinct from the mostly balanced rearrangements seen in cancer-associated chromothripsis and chromoplexy. Initially identified via chromosomal microarray analysis (CMA) and custom high-density arrays, chromoanasynthesis detection and delineation was improved by next-generation sequencing (NGS). However, the short read-lengths and read-depth variations of NGS limit its fine-mapping capabilities.

Investigating the molecular mechanisms of phytochemicals targeting Ras-related C3 botulinum toxin substrate 1 (Rac1) to inhibit breast cancer metastasis via endoplasmic reticulum stress and Wnt-PCP signaling pathway.

Context: Breast cancer metastasis remains a significant challenge in oncology, necessitating novel therapeutic approaches. Rac1 is a small GTPase involved in Wnt-planar cell polarity (Wnt-PCP) signaling and modulation of endoplasmic reticulum (ER) stress, and it has emerged as a promising target for inhibiting cancer progression. This study investigates the potential of cinnamaldehyde, a bioactive compound derived from cinnamon bark, as a potential Rac1 inhibitor.

Replication of the GWAS-Identified GALNT13 rs10196189 Polymorphism in Relation to Speed-Power Elite Active Athlete Status and Multidimensional Phenotypic Differences in Chinese Han Males: A Pilot Study.

: Previous multi-ethnic genome-wide association studies (GWAS) have identified the GALNT13 rs10196189 polymorphism as a potential genetic marker linked to sprint-power performance. However, its relevance in East Asian populations, particularly the Han Chinese, remains untested. This study aimed to replicate the association of rs10196189 with elite sprint-power athlete status in Han Chinese males and examine its potential influence on physical performance traits and tissue-specific gene regulation.

Dechlorination Triggers Defluorination: Novel Defluorination Pathways of Chlorinated Polyfluoroalkyl Substances (Cl-PFAS) by Nanoscale Zerovalent Iron.

Chlorinated polyfluoroalkyl substances (Cl-PFAS) are increasingly detected in contaminated environments, and the relative ease of C-Cl cleavage offers opportunities for defluorination. Herein, we demonstrated, for the first time, the dechlorination of Cl-PFAS by nanoscale zerovalent iron (nZVI), and we revealed that such dechlorination can induce indirect defluorination when Cl is substituted at the middle of the perfluorocarbon chain. The carbanion (-CF-CF-), generated via two successive single-electron transfer steps, is the key intermediate enabling defluorination through β-elimination of F to form alkenyl products (-CF═CF-).

β-Sitosterol preconditioning enhances the resistance of BMSCs and chondrocyte to oxidative stress and promotes cartilage repair in osteoarthritis.

Background: Osteoarthritis (OA) is a joint disorder that severely affects patients' mobility, overall health, and ability to perform daily activities. Despite advancements in therapeutic strategies, stem cell-based therapies for OA still face challenges, particularly in enhancing the antioxidative capacity of stem cells to improve therapeutic outcomes. Therefore, this study aimed to explore the potential of β-sitosterol in this context.

A Clinical-Imaging Nomogram for Predicting Early Recurrence in Patients with Solitary Hepatocellular Carcinoma After Postoperative Adjuvant TACE.

Objective: This study aimed to identify independent predictors of early recurrence (ER) and to establish a clinically applicable, individualized nomogram for patients with solitary hepatocellular carcinoma (HCC) who underwent postoperative adjuvant transarterial chemoembolization (PA-TACE).

Methods: A total of 165 patients with solitary HCC treated with PA-TACE between January 2018 and December 2022 were retrospectively analyzed. Among these patients, 71 experienced ER, while 94 remained recurrence-free for over 24 months.

Variable-Temperature Liquid ToF-SIMS Enables Molecular Investigation of Supercooled Water-in-Salt Electrolytes.

The microstructures, including ion solvation structures and water structures within supercooled water-in-salt electrolytes, determine macroscopic properties of electrolytes, such as ion conductivity, viscosity, and freezing point, thereby affecting the low-temperature operation performance of aqueous batteries. Herein, we developed an variable-temperature liquid time-of-flight secondary ion mass spectrometry (ToF-SIMS) technique to molecularly explore the evolution of microscopic interactions within the water-in-salt lithium chloride electrolyte upon supercooling as a case study. We revealed that at -20 °C, the ion solvation structures of Li(HO) and Li(HO), which are the dominant species contributing to the Li ion conductivity, undergo dehydration to form Li(HO).

The role of phytoplankton in structuring global oceanic dissolved organic carbon pools.

Phytoplankton-derived dissolved organic carbon (DOC) is a major pathway for atmospheric CO transfer to long-lived oceanic DOC reservoirs. Yet, current models rarely accounted for its molecular and taxonomic heterogeneity across growth seasons. Here, using ultra-high-resolution mass spectrometry (FT-ICR MS), we characterized DOC molecular signatures across diverse algal taxa.

Identification of quantitative trait loci and candidate genes underlying kernel traits of wheat (Triticum aestivum L.) in response to drought stress.

Fourteen environmentally stable QTLs for kernel traits were identified and shown to interact significantly with drought-stressed environments. Initial investigation of candidate genes from ten QTL clusters was undertaken, including a KASP marker for TaCYP71E1-A1 on chromosome 5A. Kernel traits, as critical yield components in wheat (Triticum aestivum L.

Scalable secondary metabolite production in Streptomyces using a plug-and-play system.

Streptomyces species are producers of bioactive secondary metabolites with clinical, agricultural and biotechnological applications. To scale these strains for industrial production, a plug-and-play system that orchestrates multitarget engineering for maximizing cellular contributions to secondary metabolites is needed. Building on the discovery that distinct quorum-sensing receptors within the Streptomyces genus can recognize an identical DNA-binding site, we build a quorum-sensing-triggered promoter applicable across various Streptomyces.

MTHFD2 drives retinoblastoma progression via Notch signaling activation: Implications for targeted pediatric ocular cancer therapy.

Retinoblastoma (RB), the most prevalent intraocular malignancy in children, severely threatens vision and survival, yet therapeutic strategies for recurrent or refractory RB remain limited. MTHFD2 (Methylenetetrahydrofolate Dehydrogenase 2), one of the key enzymes involved in mitochondrial one-carbon metabolism, is aberrantly overexpressed in multiple cancers. Elevated MTHFD2 expression correlates with poor prognosis in various malignancies, and its depletion significantly suppresses tumor invasiveness and induces programmed cell death.

Effect of perinatal sterile gloves on neonatal mortality: a post hoc analysis of a randomised clinical trial in Zambia.

Background: Sterile gloves, an inexpensive method of hand hygiene, are attributed both protective and useless characteristics concerning neonatal mortality. This study aimed to perform a post hoc secondary analysis of a cluster-randomised controlled trial (cRCT) to examine the association between intrapartum sterile gloves and neonatal mortality.

Methods: Data from the Zambia Chlorhexidine Application Trial, a cRCT of 4% chlorhexidine umbilical cord care in Zambia from 2011-2013, were analyzed.

Ultrasound characteristics of cervical lymph nodes in children with infectious mononucleosis.

Aim: To illustrate ultrasonographic characteristics of cervical lymph nodes in children with infectious mononucleosis (IM).

Material And Methods: Ultrasonographic characteristics of cervical lymph nodes in 61 children with IM were retrospectively analyzed, including the location, long diameter (LD), short diameter (SD), short-to-long diameter ratio (S/L), boundary clarity, presence of lymphatic hilum, internal hyperechoic features, blood supply mode and vascular richness.

Results: The 61 children with IM comprised 41 males and 20 females, with an average age of 7.

[Improvement effect of rehabilitation nursing based on IKAP theory on patients with urinary incontinence after radical prostatectomy].

Objective: To explore the improvement effect of rehabilitation nursing based on information-knowledge-belief-behavior (IKAP) theory on urinary incontinence patients after radical prostatectomy.

Methods: Sixty-six patients with urinary incontinence who received robot-assisted laparoscopic radical prostatectomy in General Hospital of Eastern Theater Command from January 2021 to January 2023 were selected and divided into control group (n=33) and observation group (n=33) according to random number table method. The patients in the control group were treated with rehabilitation nursing.

Prediction Model of Ki67 Proliferation Level in Hepatocellular Carcinoma Based on Contrast-Enhanced Ultrasound.

Objective: To investigate the correlation between contrast-enhanced ultrasound (CEUS) features and Ki-67 expression in patients with hepatocellular carcinoma (HCC).

Methods: It was a retrospective analysis involving 94 patients with HCC receiving surgeries and diagnosed by postoperative pathology from January 2020 to December 2021. All patients were examined by preoperative CEUS and postoperative Ki-67 immunohistochemistry.

Oxygen evolution reaction driven co-reactant-free antifouling electrochemiluminescence sensor based on electrocatalytic macromolecular heterocyclic compounds/electrically neutral hydrogel.

In this study, a co-reactant-free antifouling electrochemiluminescence (ECL) sensor was prepared based on a macromolecular heterocyclic compound electrocatalytic material and an electroneutral hydrogel. THPP as a macromolecular heterocyclic compound material, can generate reactive oxygen species (ROS). Luminal as an ECL signal source, coupled with THPP to form a THPP-Lum ECL system without co-reactants, replacing the original luminal hydrogen peroxide system, greatly simplifying the construction process of ECL sensors.

Transcriptomic reveals key genes and regulatory pathways in galactomannan biosynthesis in polysaccharide gum.

Introduction: The Lam. polysaccharide gum contained in seeds is not only an additive specified in national food standards, but also an important strategic resource for industrial raw materials such as oil and natural gas in China.The main component of polysaccharide gum is galactomannan (GM).

Analysis of the detection rate and related factors of thyroid nodules in the healthy population.

In a cross-sectional analysis of 14,973 adults from North China, thyroid nodules (TNs) were detected via high-resolution ultrasonography in 8,104 participants (54.1%), with a higher prevalence among women. The mean age of those with TNs was significantly higher (51.

Dynamic gene regulatory networks drive seed dormancy and germination of Pinus tabuliformis.

Unlabelled: The (Chinese pine), a keystone conifer species native to northern China with extended distributions into central and southern regions (e.g., Henan), plays a critical role in regional vegetation dynamics.

Legacy and Novel Per- and Polyfluoroalkyl Substances in Tap Water from East China: Impact from Water Sources and Risk Mitigation by Household Purifiers.

The growing regulatory focus on per- and polyfluoroalkyl substances (PFAS) in drinking water raises the urgent need for comprehensive monitoring and effective removal strategies. Herein, we analyzed 81 PFAS in tap water produced from three major water bodies in East China, a global PFAS emission hub. Such systematic quantitative analyses detected 64 PFAS, representing the highest reported number to date and including 28 novel species first quantified in Chinese tap water.

Transfer dynamics of extracellular antibiotic resistance genes during antibiotic and municipal wastewater treatment: contribution analysis and host identification.

The occurrence, transfer contribution, and potential hosts of intracellular and extracellular antibiotic resistance genes (ARGs) in an antibiotic and a municipal wastewater treatment plant (WTP) were comprehensively evaluated. Free-extracellular ARGs (eARGs) were dominant in effluents (59.4-79.