Identification of the malonylation modification in and insight into the regulators in biofilm formation.

Background: Post-translational modifications (PTMs) are critical regulators of bacterial biofilm formation, but the role of lysine malonylation (Kmal) in biofilm formation is still poorly understood.

Methods: In this study, we analyzed the dynamic changes of protein malonylation of () DC15 during biofilm formation based on antibody affinity enrichment combined with quantitative proteomics.

Results: Quantitative profiling identified 2,833 malonylated sites across 788 proteins, with significant enrichment in biofilm-associated proteins.

Metal Porphyrin-Terminated Hyperbranched Polyimides for Resistive Switching in Nonvolatile Memory Devices.

Polymer resistive random-access memory (RRAM) holds great promise for flexible wearable electronics and artificial intelligence, yet its development is hindered by chain entanglement and intermolecular interactions, leading to processing challenges, high operating voltages, and unstable switching parameters. Herein, metal-porphyrin-terminated hyperbranched polyimides (ATPP@HBPI, (Zn)ATPP@HBPI, and (Cu)ATPP@HBPI) were synthesized. The hyperbranched structure mitigates intermolecular interactions, while ionic doping modulates conductivity, and the synergistic effect of ions and electrons optimizes resistive switching behavior.

Safety and efficacy of CD19-targeted CAR-T-cell therapy for patients with relapsed or refractory TCF3-PBX1 fusion gene-positive B-ALL.

Chimeric antigen receptor (CAR)-T therapy has shown significant success in the treatment of relapsed or refractory acute lymphoblastic leukemia (r/r ALL). However, its role in patients with the TCF3-PBX1 fusion gene - which generally exhibit poor prognostic indicators - remains uncertain. From September 2016 to March 2023, 7 patients with r/r ALL positive for the TCF3-PBX1 fusion gene underwent CD19 CAR-T-cell therapy at the First Affiliated Hospital of Zhejiang University School of Medicine.

Cross-sectional relationship between social trust and health in middle-aged and older Chinese adults: Can social trust explain the education-health link?

Aim: Evidence on the association of social trust with health in middle-aged and older Chinese adults is limited, and its mediating role in the education-health link remains unclear. This study investigated the association of social trust with chronic diseases and mental health, and its mediating effects.

Methods: Cross-sectional data came from the 2018-2019 baseline survey of the Chongqing Cohort of the China Multi-Ethnic Cohort study, including 15 251 participants aged ≥45 years, with data collected using questionnaires and physical examinations.

Circadian disruption and ROS-NLRP3 signaling mediate sleep deprivation-enhanced silica nanoparticle toxicity in lacrimal glands.

Sleep deprivation (SD) and exposure to engineered nanomaterials such as silica nanoparticles (SiNPs) are emerging risk factors for ocular surface disorders, particularly dry eye disease. However, the molecular mechanisms underlying their combined impact on lacrimal gland function remain unclear. In this study, we investigated the synergistic effects of SD and SiNPs exposure on circadian regulation, oxidative stress, inflammation, and structural integrity of the extraorbital lacrimal glands (ELGs) in C57BL/6J mice.

Circular RNAs in Heart Diseases.

Circular RNAs (circRNAs) are a class of RNA molecules generated through back-splicing of precursor mRNAs, characterized by a covalent 3',5'-phosphodiester bond at the junction site. They are broadly categorized into exonic circRNAs, exonic-intronic circRNAs, and intronic circRNAs. CircRNAs perform diverse biological functions in various diseases, including acting as miRNA sponges, modulating protein synthesis via interactions with RNA-binding proteins (RBPs), directly influencing gene expression, and, in some cases, undergoing translation into proteins.

A novel framework for TER allocation using multilayer perceptron and intuitionistic fuzzy Z numbers for talent management.

The effective allocation of Training and Education Resources (TER) is one of many organizational pathways to maximizing workforce capability and employee development. Conventional means of assessing employees and allocating employee resources are inadequate in managing uncertainty, imprecision, or performance data in complex forms and paradigms. In this paper, a new model is proposed that implements an integrated application of Intuitionistic Fuzzy Z-Numbers and multi-layer perceptron networks for a more realistic and accurate employee performance evaluation and resource allocation.

Case Report: Papillary renal cell carcinoma complicated by ipsilateral renal tuberculosis.

Papillary renal cell carcinoma (PRCC) complicated by ipsilateral renal tuberculosis (TB) represents an exceptionally rare and complex clinical condition. Renal TB is the most common form of urogenital TB, while PRCC is the most prevalent histological subtype of non-clear cell renal cell carcinoma (RCC). In this study, we present the first reported case of PRCC complicated by ipsilateral renal TB, where the patient exhibited low back pain without hematuria.

Study on the trajectory and influencing factors of post-stroke cognitive development: based on a group-based trajectory model.

Background: Post-stroke cognitive impairment (PSCI) is one of the major complications of stroke and exhibits a dynamic progression. This study uses the Group-Based Trajectory Model (GBTM) to fit cognitive development trajectories in stroke patients and analyze the influencing factors and trends of PSCI across trajectory groups. It aims to facilitate early identification and intervention for high-risk PSCI patients, providing a theoretical basis for optimizing intervention strategies.

Peripheral nerve injury mediated by JEV strain NX1889 infection and impairment of Schwann cells.

Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus primarily associated with central nervous system disorders. Recent studies have indicated that JEV infection can lead to Guillain-Barré syndrome (GBS), a peripheral nervous system (PNS) disorder; however, the underlying mechanisms remain unclear. In this study, we investigated the tropism of different genotypes of JEV strains for peripheral nerves and their potential impact on peripheral nerves.

Characteristics of CVA16 B1c strains isolated for the first time in the Heilongjiang Province of China.

Hand, foot, and mouth disease (HFMD) represents a globally prevalent infectious disease that is caused by enteroviruses. Enterovirus A71 (EV-A71), coxsackievirus A16 (CVA16), and coxsackievirus A6 (CVA6) are recognized as the predominant causative agents of HFMD. CVA16 is a member of the genus within the family .

Safety and efficacy of autologous humanized CD19 CAR-T cell therapy for relapsed/refractory B-cell non-Hodgkin lymphoma.

Chimeric antigen receptor (CAR)-T cells have made great progress in hematological malignancies and optimization of CAR structure is a critical area of exploration. Limited research has evaluated humanized CD19-targeted CAR-T cells (hCART19) in relapsed/refractory (R/R) B-cell non-Hodgkin lymphoma (B-NHL). We conducted a clinical trial to determine the safety of hCART19 for patients with R/R B-NHL.

β-Ionone promotes ferroptosis of human gastric cancer cells by inhibiting the Wnt/β-catenin pathway in vitro and in vivo.

β-Ionone (BI) has shown the potent inhibitory malignant progression of multiple tumors including gastric cancer in vitro and in vivo. However, the mechanism by which BI inhibits gastric cancer remains to be clarified, especially in ferroptosis, which is a new form of programmed cell death. The aim of this study was to investigate whether BI could induce ferroptosis in gastric cancer and the underlying mechanisms.

The protective role of ureteral stents in reducing fibrosis and preventing stricture in ureteral injuries of different severities.

Purpose: Ureteral injuries following endoscopic interventions often lead to fibrosis and subsequent stricture formation, yet the optimal use and duration of ureteral stents in preventing these outcomes remain unclear. This study aimed to determine whether ureteral stents have an inhibitory effect on fibrosis development and provide evidence to optimize stent use and duration.

Methods: This study was conducted between March and October 2023.

Discovery of novel calcium-chelating peptides from Antarctic krill: Structural elucidation and dynamic coordination mechanisms.

Calcium-chelating peptides (CCPs) represent promising strategies for addressing calcium deficiency, yet their chelating mechanisms require further elucidation. This study identified two novel CCPs, EEDLER and IVELEEE, from Antarctic krill through enzymatic hydrolysis combined with multi-step separation and purification, exhibiting calcium-chelating capacities of 12.1 ± 1.

Multifunctional miRNA delivery systems provide synergistic neuroprotection against cerebral ischemia reperfusion injury.

Effectively alleviating cerebral ischemia-reperfusion (I/R) injury is challenging despite the medical advances. Prompt restoration of blood flow after ischemic stroke causes secondary damage to the brain tissues, triggers neuroinflammation and overproduces reactive oxygen species (ROS). MiRNA regulates the genes involved in neuron apoptosis and neuroinflammation, thus exhibiting potential in ameliorating cerebral I/R injury.

Prediction of hematoma changes in spontaneous intracerebral hemorrhage using a Transformer-based generative adversarial network to generate follow-up CT images.

Purpose: To visualize and assess hematoma growth trends by generating follow-up CT images within 24 h based on baseline CT images of spontaneous intracerebral hemorrhage (sICH) using Transformer-integrated Generative Adversarial Networks (GAN).

Methods: Patients with sICH were retrospectively recruited from two medical centers. The imaging data included baseline non-contrast CT scans taken after onset and follow-up imaging within 24 h.

Landscape of extracellular small RNA and identification of biomarkers in multiple human cancers.

Extracellular RNAs (exRNAs) in biofluids, sourced from diverse tissues, exhibit various biological functions and diagnostic potential. Small non-coding RNAs, such as rsRNAs and tsRNAs, are abundant in tissues and likely secreted into biofluids, contributing to exRNA profiles. To comprehensively evaluate exRNAs, we employed traditional and enzymatic treatment RNA sequencing to systematically profile exRNAs across six human biofluids including serum, ascites, urine, milk, seminal plasma and saliva as well as sera from mice, rats, rabbits, and bovines.

A Method Integrating the Matching Field Algorithm for the Three-Dimensional Positioning and Search of Underwater Wrecked Targets.

In this paper, a joint Matching Field Processing (MFP) Algorithm based on horizontal uniform circular array (UCA) is proposed for three-dimensional position of underwater wrecked targets. Firstly, a Marine search and rescue position model based on Minimum Variance Distortionless Response (MVDR) and matching field quadratic joint Algorithm was proposed. Secondly, an MVDR beamforming method based on pre-Kalman filtering is designed to refine the real-time DOA estimation of the desired signal and the interference source, and the sound source azimuth is determined for prepositioning.

PRC2 regulates cytokinin and HD-ZIP III pathways to orchestrate vascular tissue pattern formation in Arabidopsis.

Polycomb repressive complex 2 (PRC2) regulates plant development, but systemic PRC2 mutants have pleiotropic defects that complicate investigation of its role in regulating secondary growth. Here, we used CRISPR-TSKO to generate a vascular tissue-specific knockout of the PRC2 component FERTILIZATION INDEPENDENT ENDOSPERM (FIE) driven by the promoter of the vascular tissue-specific gene WUSCHEL HOMEOBOX RELATED 14 (WOX14), termed WOX14pro:FIE-KO, in Arabidopsis (Arabidopsis thaliana). WOX14pro:FIE-KO plants showed ectopic vascular bundles, vascular cylinders within the phloem parenchyma, and inhibited differentiation of reticulate or pitted vessels and fibers in xylem.

Macrophage Response to Avirulent and Virulent Mycobacterium tuberculosis and Anti-TB Effects of Exosome Treatment.

Tuberculosis (TB) returned as the leading cause of death from a single infectious agent in 2023. Human-macrophages and their secreted exosomes play important roles in combating invading Mycobacterium tuberculosis (Mtb). However, panoramic analysis of the underlying immune mechanism for infected macrophages, package mechanism and anti-TB effect of Mtb treated exosomes remain understood.

Exploring the Impact of Urinary Metal Exposure on Blood Lipid Profiles in Chinese Adults: Mediating Roles of Health-Related Indicators.

Background: The relationship between urinary metal exposure and lipid profiles remains unclear, particularly regarding potential mediators. This study aimed to investigate associations between urinary metals and lipid levels and to explore mediating health indicators.

Methods: We included 14 817 participants in the CMEC (Chinese Multi-Ethnic Cohort) study baseline data.

Comprehensive characterization of cold plasma/nanoparticles-functionalized gelatin active film and its application in tilapia fillets.

With the increasing demand of consumers for food preservation materials, the development of high-performance active packaging films has become a research hotspot. This study constructed a novel chondroitin sulfate-zein nanoparticles system co-encapsulating curcumin and resveratrol (ZCRC NPs) for gelatin-based active packaging, modified by atmospheric cold plasma (ACP) to achieve multifunctional enhancement. The ACP-treated composite film exhibited significantly improved in physical properties, with opacity increasing to 1.

Arginine vs tryptophan-based β-turn in the β-hairpin antimicrobial peptides: Superior antibacterial properties of the arginine-containing β-turn sequences.

The antimicrobial activity and toxicity of β-hairpin antimicrobial peptides (AMPs) primarily depend on their intramolecular β-sheet structure, which is crucially affected by the β-turn sequence. To determine whether the tryptophan or arginine motifs in the β-turn of β-hairpin AMPs exhibit greater therapeutic efficacy, a library of β-hairpin AMPs was designed based on the simplified template: (WK)X(KW)-NH (X = Trp or Arg; n = 1, 2, 3; m = 1, 2, 3). The results indicate that AMPs containing arginine motifs within the β-turn sequence exhibit superior antibacterial activity and enhanced therapeutic efficacy.

TRI-PLAN: A deep learning-based automated assessment framework for right heart assessment in transcatheter tricuspid valve replacement planning.

Background: Efficient and accurate preoperative assessment of the right-sided heart structural complex (RSHSc) is crucial for planning transcatheter tricuspid valve replacement (TTVR). However, current manual methods remain time-consuming and inconsistent. To address this unmet clinical need, this study aimed to develop and validate TRI-PLAN, the first fully automated, deep learning (DL)-based framework for pre-TTVR assessment.