Hollow Double-Layered Mesoporous Silica as an Enzyme Immobilization Platform: Enhancing Biocatalytic Efficiency in Docosahexaenoic Acid Phosphatidylcholine-Phosphatidylcholine.

This study reports the synthesis of bifunctional hollow double-layered mesoporous silica (HdlMS) nanoparticles as an effective support for enzyme catalysis. Using a self-template method combined with sol-gel processing, selective etching, and surface modification with alkyl groups and nitrogenous fatty amine groups, we successfully prepared the NH/C-HdlMS carrier. Phospholipase A1 was then immobilized onto this carrier to construct PLA1@NH/C-HdlMS, which was subsequently applied to the synthesis of docosahexaenoic-acid-enriched phosphatidylcholine.

Physiological Changes and Transcriptomics of in Response to High-Temperature Stress.

is a significant submerged macrophyte utilized in shrimp and crab aquaculture, yet it exhibits low thermotolerance. This study investigated the physiological responses and transcriptomic characteristics of under high-temperature stress (HTS). The results indicated that HTS significantly reduced the absolute growth rate (AGR) and photosynthetic efficiency of while concurrently elevating antioxidant enzyme activities, malondialdehyde (MDA) content, and concentrations of osmotic adjustment compounds.

Inhibiting SLC38A2 lowers blood pressure in rodent models of hypertension.

Hypertension remains a major global health burden with limited effective treatment options. In the present study, the sodium-dependent neutral amino acid transporter SLC38A2 was identified as a regulator of blood pressure (BP) through modulating endothelial nitric oxide (NO) signaling. Here, we show that mice with global and endothelial cell (EC)-specific gene knockout () exhibited reduced blood pressure compared with wild-type controls.

Exogenous Jaagsiekte sheep retrovirus (JSRV) Inner Mongolia strain: whole-genome characterization and viral particle packaging.

Introduction: Ovine pulmonary adenocarcinoma (OPA) is a contagious lung tumor caused by the exogenous Jaagsiekte sheep retrovirus (exJSRV). Analysing the genome of the pathogen is crucial for developing OPA prevention and control measures. Due to the absence of exogenous genomic JSRV-related information in Inner Mongolia, we aimed to establish a specific technique for exJSRV genomic amplification.

An investigation into the psychological status and influencing factors among residents undergoing standardized training.

Background: Physician mental health has become an increasingly urgent concern worldwide, yet little attention has been paid to the psychological wellbeing of resident physicians in China. This study aims to assess the mental health status of Chinese resident physicians and identify key demographic and professional factors associated with psychiatric symptoms.

Methods: A cross-sectional survey was conducted among 276 resident physicians at Qilu Hospital, Shandong University.

Molecular Surveillance and Whole Genomic Characterization of Bovine Rotavirus A G6P[1] Reveals Interspecies Reassortment with Human and Feline Strains in China.

Group A rotavirus (RVA) is a leading causative agent of diarrhea in both young animals and humans. In China, multiple genotypes are commonly found within the bovine population. In this study, we investigated 1917 fecal samples from calves with diarrhea between 2022 and 2025, with 695 testing positive for RVA, yielding an overall detection rate of 36.

High-Reynolds-Number Turbulence Database: AeroFlowData.

Turbulence widely appears in natural and industrial environments, with its multi-scale structures posing significant challenges for accurate simulation and prediction. Nowadays, turbulence databases play a crucial role in advancing scientific research. However, existing turbulence databases primarily focus on fundamental turbulence problems and are predominantly limited to turbulent flows at low-to-moderate Reynolds number, making them insufficient to address high-Reynolds-number turbulence challenges in complex engineering applications.

A Comprehensive Clinical Evaluation of Rilonacept in the Treatment of Recurrent Pericarditis: A Systematic Review.

Objective: Recurrent pericarditis (RP) is a rare cardiac condition characterized by recurrent inflammation, in which interleukin-1 (IL-1) is a key mediator. Despite increasing use of IL-1 inhibitors, comprehensive evaluations of rilonacept for RP are very limited, especially in China, where RP was newly listed as a rare disease in 2023. This review addresses the current gap by systematically assessing the efficacy, safety, cost-effectiveness, innovativeness, suitability, and accessibility of rilonacept in the management of RP, providing evidence-based guidance for clinical practice.

Influencing factors on bone mass abnormalities in Tibetan adult females from a socioeconomic perspective.

Background: Socioeconomic status significantly impacts bone health. Higher rates of abnormal bone mass in Tibetan women with lower socioeconomic status may be due to limited health services and unhealthy lifestyles. This study explores the current status and influencing factors of bone mass abnormalities in Tibetan women from a socioeconomic perspective, aiming to inform prevention and treatment strategies.

Gpr35 Expression Mitigates Neuroinflammation and Enriches Gut to Relieve Parkinson's Disease.

Parkinson's disease (PD) is associated with gut-brain axis and gut microbiota alterations, but the functioning mechanism remains to be elucidated. In this study, we identified G protein-coupled receptor 35 (Gpr35) as a key regulator for the gut-brain association under the PD context. It investigated the impact of Gpr35 deficiency on motor function, neuroinflammation, and dopaminergic neurodegeneration, using the Gpr35 knockout (Gpr35) and wild-type (WT) mice in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD model, and Gpr35 up-/down-regulation on reverse neuroinflammation, oxidative stress, and neuronal apoptosis using Gpr35 agonist kynurenic acid (KYNA) and small interfering RNA in microglial and dopaminergic cell models.

Survival outcomes in IIIC cervical cancer by treatment strategies: a systematic review and meta-analysis.

Objective: To compare survival outcomes of patients with stage IIIC cervical cancer based on different treatment strategies.

Method: Studies reporting survival of cervical cancer patients in IIIC stage were identified through systematic searches of the PubMed, Web of Science, CNKI and Wanfang data, and the hazard ratios (HRs) of survival outcomes were calculated based on different treatment strategies: radical hysterectomy (RH) group versus radical chemoradiotherapy (RCRT) group. Heterogeneity in the pooled data was assessed using the I² statistic.

PRC2 regulates cytokinin and HD-ZIP III pathways to orchestrate vascular tissue pattern formation in Arabidopsis.

Polycomb repressive complex 2 (PRC2) regulates plant development, but systemic PRC2 mutants have pleiotropic defects that complicate investigation of its role in regulating secondary growth. Here, we used CRISPR-TSKO to generate a vascular tissue-specific knockout of the PRC2 component FERTILIZATION INDEPENDENT ENDOSPERM (FIE) driven by the promoter of the vascular tissue-specific gene WUSCHEL HOMEOBOX RELATED 14 (WOX14), termed WOX14pro:FIE-KO, in Arabidopsis (Arabidopsis thaliana). WOX14pro:FIE-KO plants showed ectopic vascular bundles, vascular cylinders within the phloem parenchyma, and inhibited differentiation of reticulate or pitted vessels and fibers in xylem.

A Refined Adaptive Laboratory Evolution Strategy With Biosensor-Assisted Selection Resolves the Tolerance-Efficiency Trade-Off in Toxic Chemical Biosynthesis.

Enhancing microbial tolerance to target chemicals through conventional adaptive laboratory evolution (ALE) is time-consuming, labor-intensive, and further constrained by the challenge of balancing improved tolerance with maintaining optimal biosynthetic efficiency. Here, this work proposes a refined ALE strategy that combines initial mutagenesis with an automated microdroplet cultivation (MMC) system, thereby expediting the acquisition of tolerance phenotypes. Integrating a biosensor-assisted high-throughput screening platform enables identification of strains exhibiting advantageous "win-win" phenotypes, characterized by simultaneous improvements in both tolerance and biosynthetic capacity.

Using Artificial Intelligence to Predict Residual Distal Lumbosacral Pain Post Percutaneous Kyphoplasty for Osteoporotic Vertebral Compression Fractures.

Background: Percutaneous kyphoplasty (PKP) can restore spinal stability and relieve pain in patients with osteoporotic vertebral compression fractures (OVCF). However, in some cases, distal lumbosacral pain (DLP) persists postoperatively, affecting patients' expectations of the surgery and their recovery to activities of daily life.

Objective: To use artificial intelligence to predict DLP post-PKP for OVCF, thereby providing personalized treatment plans for patients with OVCF.

Preparation of phytosterol esters based on food-grade Pickering emulsion stabilized by lipase-adsorbed zein-hydroxypropyl chitosan nanoparticles.

This study aims to construct a food-grade Pickering emulsion stabilized by zein-hydroxypropyl chitosan composite nanoparticles (Z-HPCS NPs) adsorbed with lipase CRL (Z-HPCS NPs@CRL) to synthesize phytosterol esters. Uniform spherical Z-HPCS NPs with a strong positive charge (39.2 ± 0.

Yeast culture in weaned lamb feed: a proteomic journey into enhanced rumen health and growth.

Background: Using yeast culture as additives in ruminant feed prevents rumen microbial dysbiosis, enhances performance, and regulates rumen pH. The yeast culture used in this study was developed in-house, and has been shown to promote rumen epithelial growth in several sheep trials. Changes in protein expression associated with the promotion of rumen epithelial development following the addition of yeast culture, along with the associated molecular mechanisms, remain unknown.

Assessment of Rivaroxaban Plasma Concentration Using Chromogenic Anti-Xa Assays and UHPLC-MS/MS in Chinese Patients With Atrial Fibrillation.

Accurate quantification of rivaroxaban concentration is essential in some specific clinical situations. A prospective study was conducted to compare the rivaroxaban concentration measured by Zhenyuan anti-Xa assay with that by reference methods (ultrahigh performance liquid chromatography with tandem mass spectrometry [UHPLC-MS/MS] and Biophen DiXal) in 243 plasma samples from 182 patients with non-valvular atrial fibrillation. Zhenyuan anti-Xa assays demonstrated less bias versus reference methods in samples with concentrations exceeding 50 μg/L compared to those in the < 50 μg/L group.

High-resolution genome-wide profiling reveals intricate relationship between DNA damage and sperm physiology.

Little is known about genome-wide profiles of different types of DNA damage in spermatozoa and how they relate to sperm quality. In this study, we for the first time constructed and analyzed high-resolution profiles of two very common DNA lesions in normal human sperm. We found non-random genomic distributions of the DNA lesions and a very complex relationship between DNA damage and sperm quality that depends on the type of DNA lesion and genomic location.

Mn-Doped CeO Nanozyme-Integrated Mesoporous Interfaces for High-Sensitivity Antifouling Electrochemiluminescence Biosensing.

To address the challenges of nonspecific adsorption interference and low mass transfer efficiency encountered by electrochemiluminescence (ECL) sensors in complex biological matrices, this study developed a Mn@CeO nanozyme-based sensing interface. The Mn-doped CeO enhanced electron transfer efficiency, increased oxygen vacancy concentration, and stabilized the Mn-O-Ce structure, collectively enabling highly efficient peroxidase (POD)-like activity. The design significantly improved ECL reaction efficiency, which simultaneously conferred synergistic antifouling and mass transport enhancing properties.

Time-frequency transfer over optical fiber.

Optical time-frequency transfer establishes the metrological linkage in large-scale clock networks, which facilitates various applications. Fiber-based transfer benefits from the abundant deployment of fiber infrastructures to achieve this advantage. In this review, we provide an overview of the advances in optical two-way time-frequency transfer, which began with characterizing the time-frequency transfer stability.

Knocking down RFWD3 suppresses the growth and migration of glioblastoma cells by blocking G2/M cycle progression.

Glioblastoma (GBM) is the most common and lethal primary malignant tumor of the adult central nervous system, accounting for approximately half of all gliomas. Although RING finger and WD repeat domain 3 (RFWD3) has been implicated in the regulation of various cancers, its role and underlying mechanisms in GBM progression remain unclear. In this study, analysis of the GEPIA online database confirmed that RFWD3 expression is significantly elevated in GBM tumor tissues.

Co-NC/NbO heterostructure enable the synergistic fast sulfur redox kinetics and uniform lithium deposition for advanced lithium sulfur batteries.

Lithium‑sulfur batteries (LSBs) have attracted much attention due to their high theoretical energy density (2600 Wh kg) and low cost of sulfur cathodes. However, their practical application is hindered by the significant polysulfide shuttle effect and sluggish redox kinetics. To address these challenges, a Co-NC/NbO heterostructure was successfully prepared through high-temperature annealing of a ZIF-67 precursor followed by hydrothermal growth of NbO nanocrystals on the surface of Co-embedded and N-doped carbon polyhedrons (Co-NC).

First isolation of rabies virus from a Eurasian badger () in Inner Mongolia, China, 2024.

Rabies continues to pose a significant global zoonotic threat. In recent years, the increased spillover events of rabies viruses from wildlife to domestic animals have raised public health security concerns, prompting heightened international attention toward rabies management in wildlife populations. Our study reveals the first documented case of a rabies virus (RABV) strain isolated from Eurasian badgers () within Chinese ecosystems.

Deep Learning-Enhanced Hand-Driven Spatial Encoding Microfluidics for Multiplexed Molecular Testing at Home.

The frequent global outbreaks of viral infectious diseases have significantly heightened the urgent demand for molecular testing at home. However, the labor-intensive sample preparation and nucleic acid amplification steps, along with the complexity and bulkiness of detection equipment, have limited the large-scale application of molecular testing at home. Here, we propose artificial intelligence-enhanced hand-driven microfluidic system (MACRO) based on RPA and CRISPR technologies for home diagnosis of multiple types of infectious diseases.

Enhanced Pickering interfacial biocatalysis in phosphatidylserine synthesis via phospholipase D immobilization with optimized bifunctional strategy.

Phosphatidylserine (PS) is a high-value phospholipid in functional foods and pharmaceuticals. However, the lack of a suitable biocatalytic system has limited the conversion efficiency. A novel Pickering interfacial biocatalysis (PIB) system has developed for converting phosphatidylcholine (PC) to PS in this work.