Salicylic acid regulates biosynthesis of floral fragrance (E)-β-farnesene via NPR3-WRKY1 module in chrysanthemum.

Floral fragrance is essential for the attraction of pollinators and responses to biotic and abiotic stresses. It also enhances the quality and economic value of plants. Phytohormones, acting as key signaling molecules, are crucial roles in regulating plant growth and development.

Molecular Surveillance and Whole Genomic Characterization of Bovine Rotavirus A G6P[1] Reveals Interspecies Reassortment with Human and Feline Strains in China.

Group A rotavirus (RVA) is a leading causative agent of diarrhea in both young animals and humans. In China, multiple genotypes are commonly found within the bovine population. In this study, we investigated 1917 fecal samples from calves with diarrhea between 2022 and 2025, with 695 testing positive for RVA, yielding an overall detection rate of 36.

A prediction model based on cfDNA concentration and cfDNA methylation biomarkers for lung cancer detection.

Early detection plays a critical role in reducing lung cancer mortality. DNA methylation biomarker assay based on cell-free DNA (cfDNA) is a promising method for early detection of lung cancer. In this study, we aimed to develop a prediction model based on cfDNA methylation biomarkers and cfDNA concentration for early detection of lung cancer.

A multitemplate magnetic molecularly imprinted polymer used for highly selective separation and purification of coumarins from Angelica dahurica.

Coumarin is an important natural compound in Angelica dahurica that has high value in food, cosmetics, and medicine. In this study, a multitemplate magnetic molecular-imprinted polymer (MMIP) was synthesized via surface imprinting using FeO@SiO-C=C nanoparticles as carriers and imperatorin, isoimperatorin and oxypeucedanin as templates. The products were characterized by FT-IR spectroscopy, BET adsorption, SEM, TGA, VSM, and XRD.

A four-gene signature identified by integrated transcriptomic analysis for differential diagnosis and prognosis of uterine smooth muscle tumors.

Introduction: Uterine leiomyomas (ULM) and uterine leiomyosarcomas (ULMS) are smooth muscle tumors of the uterus that share overlapping histopathological features but exhibit markedly different biological behaviors and clinical outcomes. Whereas ULMs are benign, ULMS are rare yet highly aggressive. Clinically, accurately distinguishing tumor tissue from normal myometrial tissue remains challenging, particularly due to substantial uncertainty in preoperative diagnosis.

NEDD4 is a biomarker of a poor prognosis that contributes to the progression and chemoresistance in small cell lung cancer.

Small cell lung cancer (SCLC) accounts for approximately 15% of primary lung carcinomas and has the poorest outcome in all subtypes of lung cancer. The major hurdle for SCLC treatment failure is resistance to platinum-based chemotherapy. Therefore, an unmet need is to discover new targets that promote SCLC progression and chemoresistance.

The Regulation of Local Li Coordination Environment for High-Performance Quasi-Solid-State Polymer Electrolyte.

Quasi-solid-state polymer electrolytes (QSPEs) have attracted significant attention for their good flexibility and superior interfacial contact with electrodes, while their low room temperature ionic conductivity remains challenging. Polymer structure design and plasticizers introduction are reported to be effective in enhancing the ionic conductivity of QSPEs. However, current research predominantly focuses on enhancing lithium-ion conductivity via improved polymer chain mobility, yet critically overlooks how plasticizer/polymer-modulated Li coordination environments govern ion transport heterogeneity, ultimately constraining the achievable upper limits of ionic conduction efficiency.

Integrating scRNA-seq and spatial transcriptomics to explore the implication of G6PD on immune microenvironment in lymphatic metastasis of breast cancer.

Breast cancer is among the most prevalent malignancies, with lymph node metastasis strongly linked to worse prognostic outcomes. Despite this, the mechanisms underlying the formation of an immunosuppressive microenvironment that drives lymph node metastasis remain poorly understood. By profiling 118,127 cells from eight patients with breast cancer alongside their metastatic axillary lymph nodes through single-cell sequencing and spatial transcriptomics, this study uncovered the activation of the pentose phosphate pathway during the metastatic process.

Vertical inheritance and unique differential phenotypes of reciprocal recombinant chromosome 18 within a multi-generation family.

Carriers of balanced pericentric inversions are at risk for producing unbalanced gametes because of meiotic recombination resulting in de novo deletion and duplication of distal chromosome ends. Recombinant chromosomes generally lead to significant imbalances resulting in anomalous clinical phenotypes in offspring, hence they are typically not inherited. Therefore, the vertical transmission of recombinant chromosomes is a clinically rare event.

Brain age prediction model based on electroencephalogram signal and its application in children with autism spectrum disorders.

Background: There is a lack of objective biomarkers for brain developmental abnormalities of autism spectrum disorder (ASD). We used EEG and deep learning to conduct a brain aging study in ASD.

Methods: (1) A total of 659 healthy children and 98 ASD patients were retrospectively recruited.

Caveolin-1 inhibits the proliferation and invasion of lung adenocarcinoma via EGFR degradation.

Lung adenocarcinoma (LUAD) has become the most common pathological pattern of lung cancer in recent decades. Prediction of biological behavior of LUAD is crucial for disease management and treatment options. The objective of this study was to identify significant genes related with prognosis of LUAD, and to investigate the role and biological mechanism of caveolin-1 (CAV1) in LUAD.

The Sustainable Production of Terpenoids in Cyanobacterial Chassis.

Terpenoids, which are widely distributed in nature, possess diverse biological activities, physiological functions, and economic values. They are extensively exploited by plants and microorganisms. However, the abundance of terpenoids in natural hosts is extremely low, making it difficult to meet the market demands.

Gait Recovery in Patients with Stroke with Severe Motor Damage: The Prognostic Role of Sensory Pathway Preservation.

BackgroundThe role of sensory pathways in gait recovery remains unclear. We hypothesized that preservation of the somatosensory pathway may play a key role in gait recovery in patients with stroke and severe motor pathway impairment.ObjectiveThis study aimed to investigate the impact of preserving the somatosensory pathway, spinothalamic tract (STT), and superior thalamic radiation (STR) on gait recovery in patients with chronic stroke and severe damage to motor pathways.

Global spread of H3 subtype avian influenza viruses with an accelerated evolution after interspecies transmission.

The H3 subtype avian influenza virus (AIV) has been widely spread in birds and is known as a natural source of mammalian influenza viruses. Based on data from public databases and our surveillance data, we analyzed the ecology, evolution, and spread of H3 AIVs. Sublineages of H3 AIVs have been detected worldwide, infecting various birds, at least 90 species in wild birds and poultry.

Structural Brain Plasticity in Chronic Stroke: Cerebellar Remodeling and Its Association With Upper Limb Motor Outcome.

Background: Neuronal plasticity after stroke occurs predominantly within the first few months. However, its persistence and distribution in the chronic phase remain unclear. This study investigated longitudinal changes in cortical gray matter and corticospinal tract integrity beyond 6 months poststroke and their associations with chronic upper limb motor outcomes.

PD-1/PD-L1 inhibitors plus chemotherapy versus chemotherapy alone as the first line treatment for advanced biliary tract cancer: a pooled analysis of KEYNOTE-966 and TOPAZ-1 trails.

Background: The efficacy of PD-1/PD-L1 inhibitors plus chemotherapy (PIC) as a first-line treatment for advanced biliary tract cancer (BTC) remains controversial, given inconsistent findings regarding survival benefits and safety concerns. This meta-analysis systematically evaluates the safety and efficacy of PIC versus chemotherapy alone, aiming to provide more definitive guidance for BTC treatment decisions.

Methods: To identify phase 3 randomized controlled trials (RCTs) comparing PIC with chemotherapy alone in patients with advanced BTC, a comprehensive literature search was conducted across six databases.

Single-nucleotide polymorphisms in genes involved in folate metabolism or selected other metabolites and risk for gestational diabetes mellitus.

Background: There are conflicting results on the potential correlation between folic acid and gestational diabetes mellitus (GDM), and the correlation between genetic factors related to folic acid metabolism pathways and GDM remains to be revealed.

Aim: To examine the association between single-nucleotide polymorphisms (SNPs) of enzyme genes in the folate metabolite pathway as well as that between GDM-related genes and risk for GDM.

Methods: A nested case-control study was conducted with GDM cases ( = 412) and healthy controls ( = 412).

A Magnetron Plasma Arc Fusion Identification Study Based on GPCC-CNN-SVM Multi-Source Signal Fusion.

Plasma arc welding (PAW) is commonly employed for welding medium and thick plates due to its capability of single-side welding and double-side forming. Ensuring welding quality necessitates real-time precise identification of the melting state. However, the intricate interaction between the plasma arc and the molten pool, along with substantial signal noise, poses a significant technical hurdle for achieving accurate real-time melting state identification.

Effects of Saturated Soil Moisture on Fall Armyworm Pupal Development.

, known as the fall armyworm (FAW), a major invasive pest in corn, is rapidly spreading all over the world. Similarly to other Lepidoptera insects, FAW pupae usually develop in soil. Therefore, the soil moisture level is expected to be an important factor impacting their growth.

Brain wave dynamics in a Hopfield-Kuramoto model.

Whole brain neural oscillation activities exhibit multiple wave phase patterns and seem to be supported by the common circuit network structure. We proposed a Hopfield Kuramoto model based entirely on heterogeneous connectivity strength rather than phase delay. Multiple wave phase patterns can be encoded in heterogeneous connectivity networks via Hebbian rule and retrieved as attractors.

Disulfiram potentiates cisplatin-induced apoptosis in small cell lung cancer via the inhibition of cystathionine β-synthase and HS.

Small cell lung cancer (SCLC) is a highly malignant neuroendocrine tumor. Platinum-based chemo-resistance is the major issue for the treatment of SCLC. The objective of the study is to identify the drugs that enhance anti-tumor activity of cisplatin (CDDP) in SCLC.

Characterization of the avian influenza viruses distribution in the environment of live poultry market in China, 2019-2023.

Background: The prevalence and transmission of avian influenza viruses (AIVs) in the live poultry market (LPM) is a serious public health concern. This study was to investigate the prevalence of different subtypes of avian influenza viruses in environment of LPM, and to analyze the differences and seasonality of the nucleic acid positive rate (NAPR) of A type, H5, H7, and H9 subtypes in feces, sewage, drinking water, breeding cages, and chopping boards.

Methods: Feces, breeding cages swabs, drinking water, sewage and chopping boards swabs were collected from live poultry market during 2019-2023 from southern and northern China.

Establishing a Risk Prediction Model for Nasopharyngeal Carcinoma Based on Anti-BNLF2b Serological Biomarkers: A Retrospective Study.

This study aims to establish a suitable risk prediction model of NPC in regions with relatively low-incidence in southern China. We retrospectively analysed the data of 198 patients with NPC and 398 healthy individuals admitted to The Second Affiliated Hospital, Zhejiang University School of Medicine, from February 2023 to October 2024. The levels of different serum biomarkers (P85-Ab, VCA-IgA, VCA-IgM, VCA-IgG, Rta-IgG and EA-IgA) were compared between patients with NPC and healthy individuals.

Single-nucleus RNA sequencing and spatial transcriptomics reveal an immunosuppressive tumor microenvironment related to metastatic dissemination during pancreatic cancer liver metastasis.

Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy characterized by early liver metastasis and high mortality. The tumor microenvironment plays a pivotal role in tumor progression; however, the immune microenvironment's involvement in PDAC liver metastasis remains poorly understood. This study investigates cellular heterogeneity in primary tumor (PT) and liver metastasis (LM) tissues of PDAC using single-nucleus RNA sequencing and spatial transcriptomics.

Helicobacter pylori reversing the landscape of neoadjuvant immunotherapy for microsatellite stable gastric cancer: a multicenter cohort study.

Background: Microsatellite stable (MSS) gastric cancer (GC) is largely unresponsive to immunotherapy, presenting a persistent and formidable challenge in the field. Patients with advanced GC and Helicobacter pylori (H. pylori) infection have shown benefits from immunotherapy.