The global, regional, and national disease burden of breast cancer attributable to behavioral risks from 1990 to 2021 and projections to 2035: a systematic analysis of the Global Burden of Disease Study 2021.

Background: Behavioral factors have been identified as crucial contributors to breast cancer (BC) risk, exhibiting distinct patterns across different regions, sexes, and age demographics. This study aims to analyze the spatiotemporal trends of behavior-related breast cancer (BRBC) at the global, regional, and national scales from 1990 to 2021, while also forecasting its future trajectory up to 2035.

Methods: Utilizing the Global Burden of Disease 2021 data, we assessed BC mortality and disability-adjusted life years (DALYs) attributable to tobacco, alcohol, diet, and physical inactivity.

CD4 T Cell Subsets and as Novel Biomarkers of Immune Dysregulation in Dilated Cardiomyopathy.

Recent multiomics advancements have improved our understanding of immune dysregulation in dilated cardiomyopathy (DCM). However, specific immune cell subsets and their regulatory genes are still ambiguous. This study aimed to explore immune cell imbalances and regulatory genes in DCM, discover diagnostic biomarkers, and identify potential therapeutic targets.

Codon Usage Pattern of Papillomavirus E6, E7, and L1 Genes Across Hosts.

This study investigates the codon usage preferences of papillomaviruses across different hosts and examines the evolutionary relationships of key genes (L1, E6, and E7). Complete genome sequences of 86 papillomavirus strains were retrieved from the PaVE database. Phylogenetic trees were constructed using MAFFT and ITOL, and codon usage indices, including RSCU, ENC, CAI, and GC3, were calculated using CodonW.

Analysis of ischemic intestinal tissue composition based on visible and near-infrared reflectance hyperspectral imaging and multivariate curve resolution.

Acute mesenteric ischemia (AMI) is a life-threatening, rapidly progressive disease. Conventional diagnostic methods have their own limitations. Therefore, a non-invasive, real-time and efficient diagnostic method is needed.

An efficient elliptic curve-based deterministic measurement matrix for micro-seismic data acquisition.

Micro-seismic monitoring plays a critical role in geological disaster prediction. Current compressed sensing approaches for micro-seismic data acquisition, however, are hampered by measurement matrices with high computational complexity and inadequate reconstruction accuracy, particularly in resource-constrained sensor networks. To overcome these limitations, we propose an novel deterministic measurement matrix based on elliptic curve pseudo-random sequences.

Expanding the cytokine receptor alphabet reprograms T cells into diverse states.

T cells respond to cytokines through receptor dimers that have been selected over the course of evolution to activate canonical JAK-STAT signalling and gene expression programs. However, the potential combinatorial diversity of JAK-STAT receptor pairings can be expanded by exploring the untapped biology of alternative non-natural pairings. Here we exploited the common γ chain (γ) receptor as a shared signalling hub on T cells and enforced the expression of both natural and non-natural heterodimeric JAK-STAT receptor pairings using an orthogonal cytokine receptor platform to expand the γ signalling code.

Comparing the Effects of Fentanyl, Sufentanil, and Butorphanol Combined With Flurbiprofen Axetil on Postoperative Intravenous Patient-controlled Analgesia Post Cesarean Delivery.

Background: The analgesic effects between fentanyl, sufentanil, and butorphanol combined with flurbiprofen axetil on postoperative patient-controlled intravenous analgesia (PCIA) after cesarean delivery has never been evaluated.

Objectives: To evaluate the postoperative analgesic efficacy of selected PCIA formulae.

Study Design: This is a retrospective study.

Transcriptomic insights into photoperiodic regulation of goose reproduction: galanin emerges as a potential player.

[OBJECTIVE]: To characterize hypothalamic mRNA profiles under different photoperiods and identify key reproductive regulators based on the "ultralong-short-long" artificial photoperiodic reproduction model of practical production. [METHODS]: The triphasic photoperiodic regimens consisted of ultralong (L:D = 20 h:4 h), short (L:D = 6.5 h:17.

De novo design and structure of a peptide-centric TCR mimic binding module.

T cell receptor (TCR) mimics offer a promising platform for tumor-specific targeting of peptide-major histocompatibility complex (pMHC) in cancer immunotherapy. In this study, we designed a de novo α-helical TCR mimic (TCRm) specific for the NY-ESO-1 peptide presented by human leukocyte antigen (HLA)-A*02, achieving high on-target specificity with nanomolar affinity (dissociation constant = 9.5 nM).

Enhancement of 3-MA in Paclitaxel Treatment of MDA-MB-231 Tumor-Bearing Nude Mice and Its Mechanisms.

Triple-negative breast cancer (TNBC) poses significant challenges due to its high aggressiveness, poor prognosis, and the lack of effective targeted therapies. Paclitaxel (PTX) is a chemotherapeutic agent commonly used in the treatment of TNBC; however, its efficacy is often compromised by drug resistance mediated by autophagy. This study investigated the synergistic effects of the autophagy inhibitor 3-methyladenine (3-MA) and PTX in a TNBC nude mouse model.

Mice with a diverse human T cell receptor repertoire selected on multiple HLA class I molecules.

T cell receptor (TCR) gene therapy is an effective cancer treatment. Ideally, the TCR should be of human origin and have optimal avidity, e.g.

Screening for Heart Failure in Patients with Hypertension And/Or Diabetes Using Hand-Held Echocardiography: A Pilot Study.

Objective: This study aimed to assess the feasibility and cost-effectiveness of hand-held echocardiography-based screening for Stage B or C heart failure among individuals with hypertension and/or diabetes at the High-tech Area Fangcao Community Health Service Center and the Tianfu New Area Huayang Community Health Service Center in Chengdu, China, with the objective of promoting early diagnosis and intensified care.

Methods: Patients with hypertension and/or diabetes registered and cared for at two community health service centers (CHSCs; Chengdu, China) with no history of clinical heart failure were recruited between October 2021 and December 2021. By combining symptom assessment (dyspnea and/or edema) and N-terminal-probrain natriuretic peptide (NT-proBNP ≥ 125 pg/ml as the cut-off) levels with HHE for any indexed abnormality in the dedicated semi-quantitative protocol, patients were categorized into heart failure (HF) Stages A, B, and C.

Global spread of H3 subtype avian influenza viruses with an accelerated evolution after interspecies transmission.

The H3 subtype avian influenza virus (AIV) has been widely spread in birds and is known as a natural source of mammalian influenza viruses. Based on data from public databases and our surveillance data, we analyzed the ecology, evolution, and spread of H3 AIVs. Sublineages of H3 AIVs have been detected worldwide, infecting various birds, at least 90 species in wild birds and poultry.

ANG secretion predisposes endothelial cells toward angiogenesis in FIN56-induced ferroptotic hepatocellular carcinoma via the BMP6/ID1 signaling pathway.

Background: Hepatocellular carcinoma (HCC) poses significant challenges due to its high malignancy and limited treatment options. FIN56 has emerged as a potent inducer of ferroptosis, yet its precise mechanism of action in HCC remains elusive.

Methods: Ferroptosis induction by FIN56 in HCC cells was assessed by quantifying malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), reactive oxygen species (ROS), glutathione (GSH) and Liperfluo levels, as well as evaluating changes in mitochondrial morphology.

Multi-analyte approach combining cfDNA sequencing and protein testing for early ovarian cancer detection.

Non-invasive liquid biopsy is a promising strategy for ovarian cancer (OC) detection. We evaluated the feasibility and efficacy of a multi-analyte approach combining circulating tumor DNA (ctDNA) detection with protein biomarkers for early OC detection. At 95% specificity, CA125 and ctDNA alone exhibited an overall sensitivity of 79.

Solution mapping of MHC-I:TCR interactions using a minimalistic protein system.

Recognition of epitopic peptide antigens presented on class I major histocompatibility complex (MHC-I) proteins by T cell receptors (TCRs) forms the cornerstone of immune surveillance, leading to a plethora of adaptive immune responses. Characterization of TCR:peptide/MHC-I interactions is critical for understanding immune recognition, and developing immunotherapies, but the large variation in docking orientations of TCRs on their peptide/MHC-I targets challenges structural modeling. NMR spectroscopy could potentially resolve this ambiguity, but the large size of the TCR:peptide/MHC-I complex limits data quality.

Toll-like receptor-4 in the fish immune system.

Toll-like receptors (TLRs) represent a critical class of protein molecules integral to nonspecific immunity (innate immunity), and serve as key mediators bridging between nonspecific and specific immune responses. As an important member of the TLR multigene family, toll-like receptor 4 (TLR4) holds particular significance due to its broad expression across various animal tissues and its pivotal roles in pathogen recognition, signal transduction, and the modulation of inflammatory responses. In aquaculture, Tlr4 has emerged as a promising target for the development of vaccines, immune enhancers, and adjuvants aimed at bolstering the health and disease resistance of aquatic animals, thereby reducing the reliance on antibiotics.

Knockdown of TRIM47 Overcomes Paclitaxel Resistance in Ovarian Cancer by Suppressing the TGF-β Pathway via PPM1A.

Objective: We investigated the role of tripartite motif 47 (TRIM47) in paclitaxel resistance in ovarian cancer, focusing on its regulation of protein phosphatase magnesium-dependent 1A (PPM1A), transforming growth factor-β (TGF-β) pathway activation, and methyltransferase-like 3 (METTL3)-mediated N6-methyladenosine (m6A) modification.

Methods: Bioinformatics analysis using Gene Expression Profiling Interactive Analysis (GEPIA), Kaplan-Meier plotter (KM plot), Linkedomics, and sequence-based RNA adenosine methylation site predictor (SRAMP) databases identified TRIM47 and PPM1A expression patterns, prognostic significance, co-expression networks, and m6A modification sites. Paclitaxel-resistant ovarian cancer cell lines were generated.

Ion-modulated graphene oxide and GO@MXene hydrogels: enhanced adsorption performance and stability for methylene blue removal.

Ion cross-linking is often used to prepare graphene hydrogels, but the effects of different ion introductions on the properties of graphene hydrogels need to be further explored. In this study, graphene oxide (GO) and GO@MXene non-spherical hydrogels were prepared by introducing different ions, and their adsorption effects on methylene blue (MB) were discussed to determine their feasibility in water treatment. The adsorption efficiencies were significantly variable because the introduction of different ions had different effects on the internal structure and molding process of the hydrogels.

Association between hemoglobin, albumin, lymphocyte, and platelet score and all-cause and cardiovascular mortality among population with diabetes: Evidence from the NHANES 2003-2016.

Introduction: This study investigated the relationship between the Hemoglobin, Albumin, Lymphocyte, and Platelets (HALP) score and all-cause and cardiovascular mortality risk in diabetes patients.

Methods: An analysis included 2154 individuals with diabetes from the 2003-2016 National Health and Nutrition Examination Survey (NHANES), with mortality data tracked until December 31, 2019. Cox regression models were adopted to evaluate the association of HALP score with mortality.

The INAVA mRNA in Extracellular Vesicles Activates Normal Ovarian Fibroblasts by Phosphorylation-Ubiquitylation Crosstalk of HMGA2.

Ovarian cancer is an aggressive gynecological tumor usually diagnosed with widespread metastases. Extracellular vesicles (EVs), though recognized as important mediators of tumor metastasis, have received limited attention into their specific functions via the mRNA profiling. Here it is reported elevated expression and selective enrichment of INAVA mRNA in both plasma- and tissue-derived EVs from ovarian cancer patients, which is positively correlated with distant metastasis and poor prognosis.

Changes in goose hypothalamus under different photocycles: RNA-Seq reveals new pathways and molecules.

Reproduction in avian species is regulated by light, and the hypothalamus is the most important regulator of the response to light signals. The objective of this study was to identify the key molecules and signaling pathways in the hypothalamus that regulate reproduction in birds based on the photoperiod. Yangzhou geese kept in a closed goose barn were selected for transcriptome sequencing.

Identification of Near-Infrared Characteristic Bands of Small Intestine Necrosis Based on Least Trimmed Squares With Regularization.

Hyperspectral imaging is a promising tool for identifying ischemic necrotic small intestine. To analyze the causes of small bowel necrosis, studying characteristic bands is crucial. However, differences in samples and spectral acquisition devices limit the availability of all bands for analysis, posing challenges in selecting bands adapted to individual variations.

Construction and evaluation of machine learning-based prediction model for live birth following fresh embryo transfer in IVF/ICSI patients with polycystic ovary syndrome.

Objective: To investigate the determinants affecting live birth outcomes in fresh embryo transfer among polycystic ovary syndrome (PCOS) patients using various machine learning (ML) algorithms and to construct predictive models, offering novel insights for enhancing live birth rates in this specific group.

Methods: A sum of 1,062 fresh embryo transfer cycles involving PCOS patients were analyzed, with 466 resulting in live births. The dataset was split randomly into training and testing subsets at a 7:3 ratio.

Mineralized Bacteria as a Potent Vaccine Against Staphylococcus aureus Infections.

Staphylococcus aureus (S. aureus) as common Gram-positive pathogenic bacteria, causes local and systemic infections, including sepsis and bacteremia. In particular, the high prevalence of drug-resistant S.