Synaptic vesicle protein 2A regulates mossy fiber sprouting in a drug-resistant epilepsy rat model via laminin α5/integrin β1.

Drug-resistant epilepsy (DRE) is frequently characterized by pathological mossy fiber sprouting (MFS), which is a defining indicator of aberrant synaptic remodeling within the hippocampus. Despite extensive investigations of the molecular underpinnings of MFS, they remain only partially elucidated. Synaptic vesicle protein 2A (SV2A) is a key modulator of neurotransmitter exocytosis that has been associated with epileptogenesis.

Evidence for vertical transmission of avian reovirus in chickens.

Avian reovirus (ARV) has emerged as a highly prevalent pathogen in Chinese poultry, characterized by its high mutation rate, environmental resilience, and dual vertical-horizontal transmission routes, leading to rising chick morbidity and substantial economic losses. To evaluate vertical transmission, 945 dead embryos and 58 weak chicks from a Guangdong hatchery between January 2023 and December 2024 were tested, yielding a 9.6 % (96/1003) ARV positivity rate.

Protective effect of liquiritin against cisplatin-induced liver injury in mice through reduction of inflammation, oxidative stress, apoptosis and inhibition of p38 MAPK/p53 pathway based on network pharmacology and in experimental validation.

Ethnopharmacological Relevance: Cisplatin is widely used in chemotherapy, but hepatotoxicity limits its effectiveness. Liquiritin has liver-protective effects, but its mechanisms have not been elucidated in combination with cisplatin for liver-injury treatment in mice.

Aim Of The Study: This investigation explored liquiritin's protective effect against cisplatin-induced liver damage in mice and its mechanisms.

Transcatheter aortic valve implantation in a patient with isolated aortic regurgitation using JenaValve.

This case report details the successful use of JenaValve for transcatheter aortic valve implantation in a male patient in his early 50s with severe aortic regurgitation. The patient had multiple comorbidities, including chronic kidney disease and heart failure, increasing the risk of surgical intervention. JenaValve, a second-generation device with unique positioning and anchoring mechanisms, was deployed successfully, resulting in immediate improvements in cardiac function without significant regurgitation or paravalvular leakage.

Alternative Splicing of the NMDA Receptor Subunit GluN1 Mediated by Polypyrimidine Tract-Binding Protein Dimerization in the Trigeminal Ganglion Contributes to Orofacial Allodynia.

Orofacial neuropathic pain, a debilitating condition associated with trigeminal nerve injury, is often characterized by allodynia. N-methyl-d-aspartate receptors (NMDARs), particularly the GluN1 subunit, play a central role in mediating this pain. The GluN1 subunit undergoes alternative splicing at exon 5, generating isoforms GluN1a (lacking the exon 5-encoded N1 cassette) and GluN1b (retaining the N1 cassette), which have distinct functional roles.

Peripheral blood mesenchymal stem cell-derived exosomes improve renal sympathetic denervation efficacy through β-catenin-mediated cardiac reprogramming.

Background: To investigate the role of self-peripheral blood mesenchymal stem cell (PBMSC)-derived exosomes (Exos) in enhancing renal sympathetic denervation (RD)-mediated heart regeneration following myocardial infarction (MI) in a porcine model.

Methods: Pigs (ejection fraction [EF] < 40% post-MI) were randomised to early sham RD or RD. At 2 weeks post-MI, autologous PBMSC-Exos were collected.

Developing a prognostic model of glutamine metabolism-related genes associated with clinical features and immune status in melanoma.

Introduction: Melanoma exhibited a poor prognosis due to its aggression and heterogeneity. The effect of glutamate metabolism promoting tumor progression on cutaneous melanoma remains unknown. Herein, glutamine metabolism-related genes (GRGs) were identified followed by constructing a prognostic model for melanoma via bioinformatics analysis.

Processing bodies promote lysosomal quality control and cell survival during recovery from lysosomal damage.

Lysosomes are essential for cell survival but are highly susceptible to diverse physical and pathological stressors. Thus, the ability to initiate an acute damage response and promote recovery after stressor resolution is critical for maintaining cellular homeostasis and viability. Although recent studies have advanced our understanding of acute responses to lysosomal injury, the molecular mechanisms governing the recovery stage and distinguishing it from the acute phase remain poorly defined.

Molecular Stacking Patterns Enhance Organic Small-Molecule Electrochemical Stability and Enable Ion Separation.

Organic small-molecule materials, leveraging their multisite nature, low molecular weight, sustainability, and element-rich composition, are promising candidates for electrochemical ion extraction applications. However, restricted structural stability, caused by ion-intercalation-induced volume expansion and resulting capacity decay, has hindered further application. Here, based on a structural stacking approach to form an integrated intermolecular force network and lithiophilic ion channels, phenazine (PNZ) is utilized to demonstrate the significant functional relevance of molecular stacking structures in enhancing organic small-molecule electrochemical stability.

Association of leucocyte telomere length with incident age-related macular degeneration: a prospective UK Biobank Study.

Purpose: There have been conflicting findings on the role of leucocyte telomere length (LTL) in the risk of age-related macular degeneration (AMD). In this study, we evaluated the associations between LTL and the risk of incident AMD and explored whether age, sex and/or genetic predisposition to AMD can modify these associations.

Methods: We conducted a longitudinal cohort study involving 332 123 AMD-free participants with complete baseline covariates and LTL data from the UK Biobank.

DNA methylation of the ABCA1 and ABCG1 genes contribute to stroke risk.

Introduction: Stroke is a major cause of death in China. The epigenetic factors, especially the methylation of ABCA1/G1 genes implicated in cholesterol regulation, are being examined to comprehend their association with stroke.

Methods: In this nested case-control study, we examined data from the North-west China Cohort (CNC-NX) initial phase, involving 53 pairs of stroke cases and controls.

Association of cerebrospinal fluid FGF21 with sleep quality in Chinese adults.

Objective: This study aimed to examine whether FGF21 levels in cerebrospinal fluid (CSF) and plasma are associated with sleep quality, as measured by the Pittsburgh Sleep Quality Index (PSQI) scores, and to assess the correlation between FGF21 levels and sleep disorder severity.

Methods: We recruited 90 Chinese adult males undergoing anterior cruciate ligament reconstruction surgery, gathering sociodemographic and clinical characteristics. Sleep quality was assessed using the PSQI, and CSF samples were obtained for FGF21 analysis.

Squama Manitis Extract Exhibits Broad-Spectrum Antibacterial Activity Through Energy and DNA Disruption Mechanisms.

The global antimicrobial resistance crisis demands innovative strategies to combat bacterial infections, including those caused by drug-sensitive pathogens that evade treatment through biofilm formation or metabolic adaptations. Here, we demonstrate that Squama Manitis extract (SME)-a traditional Chinese medicine component-exhibits broad-spectrum bactericidal activity against clinically significant pathogens, including both Gram-positive () and Gram-negative () species (MIC = 31.25 mg/mL), achieving significant reduction in bacterial viability within 24 h.

Internal Delivery and Transport within Cellular Aggregates via Perfusable Glass-Sheathed Hydrogel Microtubes.

Measuring the transport dynamics of soluble molecules such as nutrients, growth factors, and therapeutics within cell aggregates is essential to understand the transport-limiting effects of 3D cell culture models. Traditional methods to study molecular transport within engineered tissues often face challenges related to access for delivery and sampling and require sacrificing the culture. Here, we introduce an accessible, device-innovation platform that allows spatially defined delivery into a living cell aggregate.

V-Induced Low-Spin State Mn Suppresses Jahn-Teller Distortion for High-Performance Aqueous Zinc Ion Batteries.

The Jahn-Teller distortion caused by high-spin state Mn (t e ) is a major limiting factor for improving both the specific capacity and cycling stability of MnO cathodes in aqueous zinc-ion batteries. Thus, an intrinsic strategy for optimizing MnO involves the effective elimination of the high-spin state Mn (t e ) during electrochemical process. Herein, we focus on structural design that constructed NHVO-coated MnO (Mn@V) nanorods to achieve the low-spin state of Mn (t e ) and inhibit the Jahn-Teller distortion.

Mechanism of Microbiota-Gut-Brain in Perimenopausal Depression: An Inflammatory Perspective.

Background: Perimenopausal women often experience physiological and psychological decline due to the effects of oestrogen fluctuations and the decline of ovarian function, leading to significantly increased depression rates, decreases in the quality of life and mental health issues. Studies have shown that the gut microbiota exerts anti-perimenopausal depression (PMD) effects via the microbiota-gut-brain (MGB) axis, the mechanisms of which may be related to inflammation. In this review, we discuss the effects and mechanisms of gut microbiota in PMD and provide new insights for future PMD treatment.

Graphene oxide-based dual-color thin-film fluorescent nanolabels for enhanced immunochromatographic detection of pesticides.

Background: The excessive use of pesticide pollutants in agricultural production seriously threatens food safety. Traditional detection techniques are difficult to meet the detection requirements due to the complex sample pretreatment and high detection costs. The immunochromatography method (ICA) is simple to operate and fast, and is suitable for on-site rapid detection.

Role of LPC 18:1 in the Lipid Metabolic Response to Combined Vitamin D and Resistance Exercise for Protecting Diabetic Skeletal Muscle Function.

Purpose: We have previously found that vitamin D and resistance exercise synergistically improve type 2 diabetes mellitus (T2DM)-related skeletal muscle atrophy. This study aims to investigate the impact of varying exercise intensity on synergistic effects of vitamin D and resistance exercise on their efficacy in improving type 2 diabetes mellitus (T2DM)-induced myopathy, and further elucidate the underlying mechanism.

Methods: We compared the effects of vitamin D combined with low-, moderate- and high-intensity resistance exercise on metabolic status and skeletal muscle function.

Sulfonation-modified Artemisia sphaerocephala gum films: Fabrication strategies and characterization.

To enhance the bioactivity and expand the application scope of Artemisia sphaerocephala Krasch polysaccharide gum (ASKG), it was sulfonated to yield sulfonated ASKG (S-ASKG). Using ASKG as a control, comparative analyses of the physicochemical properties and functional characteristics were performed. Films fabricated from S-ASKG and ASKG were comparatively analyzed for their structural, mechanical, and barrier properties.

Effectiveness of various exercise on neuropsychiatric symptoms among older adults with mild cognitive impairment or dementia: A systematic review and network meta-analysis.

Objective: To identify the comparative efficacy of exercise for reducing neuropsychiatric symptoms (NPS) among older adults with mild cognitive impairment (MCI) or dementia.

Methods: Ten databases were systematically searched from their inception to April 29, 2025, with the latest update in July 13, 2025. Randomized controlled trials (RCTs) and quasi-experimental studies evaluating the effectiveness of exercise on NPS in older adults with MCI or dementia were included.

Gut microbiota dysbiosis exacerbates polystyrene microplastics-induced liver inflammation via activating LPS/TLR4 signaling pathway in ducks.

Ubiquitous microplastics can bioaccumulate in organisms, resulting in detrimental health impacts, such as liver inflammation. Nonetheless, the exact mechanism by which polystyrene microplastics (PS-MPs) trigger liver inflammation via the gut-liver axis in ducks remains unclear. The purpose of this study was to clarify the impact of PS-MPs exposure to liver inflammation through the gut-liver axis in ducks.

Case Report: Concurrent retinal vasculitis and optic neuritis in systemic lupus erythematosus.

Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease that can affect the ocular system, with retinal vasculitis and optic neuritis being rare but serious manifestations. We present a case of a 26-year-old female with newly diagnosed SLE who developed both retinal vasculitis and optic neuritis, leading to progressive visual impairment. She was successfully treated with methylprednisolone and rituximab, achieving significant visual recovery.

The bittersweet link between glucose metabolism, cellular microenvironment and viral infection.

Viral particles and proteins released during infection profoundly reshape the cellular microenvironment by disrupting host signaling, triggering inflammation, and modulating immune responses. Glucose metabolism, a critical hub for energy production and biosynthesis, is highly susceptible to viral reprogramming. This review summarizes recent findings showing that diverse viruses, including influenza virus, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and enteroviruses, manipulate glucose metabolic pathways to promote replication and evade immune surveillance.

Atypical lipomatous tumor/well-differentiated liposarcoma of the breast: A rare case report.

Rationale: Liposarcoma, the most common type of soft tissue sarcoma, typically occurs in the deep soft tissues of the limbs and retroperitoneum and is rarely found in the breast. The fifth edition of the World Health Organization classifies it into 5 distinct histological subtypes. Given the rarity of liposarcomas in breast tissue, there remain certain challenges in diagnosis and treatment planning.

Ligand-mediated structural modulation and membrane stabilization of LPA1 receptor: Insights from molecular dynamics simulations.

Lysophosphatidic acid (LPA) is an important bioactive signaling molecule that activates six distinct G protein-coupled receptors (GPCRs), among which the LPA1 subtype possesses high therapeutic target potential due to its critical roles in malignant tumors, pulmonary fibrosis, inflammation, and neuropathic pain. Recent studies have shown that the small molecule ONO-0740556 can effectively disrupt the overall structure of LPA1 in solution; however, its effects in a native membrane environment remain unclear. According to the Traditional Chinese Medicine Systems Pharmacology Database (TCMS), Dan Shen(Salvia miltiorrhiza) exhibits anti-inflammatory and anti-pulmonary fibrosis properties, and its natural compound cryptoxanthin may serve as a novel LPA1 inhibitor.