Molecular Stacking Patterns Enhance Organic Small-Molecule Electrochemical Stability and Enable Ion Separation.

Organic small-molecule materials, leveraging their multisite nature, low molecular weight, sustainability, and element-rich composition, are promising candidates for electrochemical ion extraction applications. However, restricted structural stability, caused by ion-intercalation-induced volume expansion and resulting capacity decay, has hindered further application. Here, based on a structural stacking approach to form an integrated intermolecular force network and lithiophilic ion channels, phenazine (PNZ) is utilized to demonstrate the significant functional relevance of molecular stacking structures in enhancing organic small-molecule electrochemical stability.

The Application of the Prevertebral Fascia in the Surgical Approach to the Craniocervical Junction: Cadaveric Dissection Studies and Clinical Case Demonstrations.

Background: The suboccipital muscles at the craniocervical junction exhibit high anatomical complexity. Inadequate use of correct anatomical planes during surgery complicates exposure of deep structures and increases the risk of damaging the carotid sheath (CS) and parotid gland (PG). This study aims to identify key anatomical landmarks for protecting the PG and optimizing CS exposure in far lateral approach (FLA) and extreme lateral approach (ELA).

Detecting biliary complications following liver transplantation with contrast-enhanced ultrasound.

Liver transplantation is the primary therapeutic choice for end-stage liver disease. Currently, biliary complications are among the main factors affecting the survival rate and quality of life of liver transplant recipients. Nevertheless, the clinical manifestations of biliary complications following liver transplantation are often non-specific, making early diagnosis and timely treatment crucial for improving patient outcomes.

Metabolic reprogramming and prognostic insights in molecular landscapes driven by glycolysis in ovarian cancer.

Ovarian cancer (OC) is a highly fatal gynecological malignancy primarily attributable to late-stage detection and restricted treatment options. Aberrant glycolysis, exemplified by the Warburg effect, facilitates tumor development, immunological evasion, and alteration of the microenvironment. Identifying glycolysis-related biomarkers could provide novel insights into prognosis and potential therapeutic targets for OC.

Toward bridging the gap between machine intelligence and machine wisdom: Dilemmas and conjectures.

In recent years, artificial intelligence (AI) has achieved tremendous development, akin to a significant leap, similar to progressing from 1 to 100. However, a significant gap still exists between current machine intelligence and human wisdom: machine intelligence is constrained to post hoc inference based on existing data, lacking the ability for genuine exploratory innovation and possessing no prospective reasoning inherent to human wisdom. Drawing inspiration from human wisdom, this article presents conjectures for overcoming the four dilemmas faced by machine intelligence: neglect of silicon-based cognition, lack of artistry, pitfall of perfectionism, and obsession with uniformity.

Platelets in infection: intrinsic roles and functional outcomes.

Platelets have long been acknowledged for their essential roles in hemostasis and thrombosis; however, recent insights highlight their broader involvement as key participants in host responses during infection. Beyond their classical functions, platelets exhibit diverse anti-infective capabilities, such as direct pathogen internalization, receptor-mediated pathogen recognition, the release of antimicrobial peptides, cytokines, and chemokines, and the generation of immunomodulatory extracellular vesicles. These intrinsic platelet attributes enable dynamic interactions with pathogens and immune cells, significantly contributing to pathogen capture, neutralization, and the orchestration of innate and adaptive immune responses.

Expansion-Floating Craniotomy as a Novel Alternative Technique for Decompressive Craniectomy: A Technical Note.

Background: Decompressive craniectomy and subsequent cranioplasty are associated with significant morbidity and mortality. This study presents a novel expansion-floating craniotomy designed to provide a decompressive volume similar to decompressive craniectomy and allows the bone flap repositioning later in a simple way.

Methods: In expansion-floating craniotomy, the bone flap was elevated with 3 plates to the desired position to ensure sufficient decompression and preserve capacity for subsequent outward displacement.

A SERS aptasensor based on Au@Ag bimetallic nanostars-magnetic covalent organic composites for rHuEPO-α detection.

Background: The misuse of recombinant human erythropoietin-alpha (rHuEPO-α) for performance enhancement in sports has been a persistent issue. The World Anti-Doping Agency has emphasized the need for reliable detection methods to identify the illicit use of rHuEPO-α, thereby upholding the integrity of sports competitions. Most screening strategies rely on immunological methods for detection.

Design and immunogenic evaluation of multi-epitope vaccines for colorectal cancer: insights from molecular dynamics and studies.

Background: This study aimed to identify cytotoxic T lymphocyte (CTL)-specific epitopes from three tumor-associated antigens (TAAs)-Dickkopf-like 1 (DKKL1), F-box protein 39 (FBXO39), and Opa-interacting protein 5 (OIP5)-which are overexpressed in colorectal cancer (CRC), as potential candidates for CTL-mediated immunotherapy.

Methods: The amino acid sequences of DKKL1, FBXO39, and OIP5 were analyzed to predict high-affinity CTL epitopes using the NetCTL server. Their antigenicity, allergenicity, conservation, and glycosylation potential were assessed for safety and effectiveness.

Machine learning-driven prediction of immune checkpoint inhibitor responses against cholangiocarcinoma: a bile biopsy perspective.

Background: The treatment of cholangiocarcinoma (CCA) continues to face numerous clinical challenges, including the prediction of sensitivity to immunotherapy and the development of preoperative diagnostic models.

Methods: In this study, we aimed to address these challenges by collecting bile samples from CCA patients for metabolomic and microbiomic analyses. We also performed immunofluorescence (IF) staining on tissue formalin-fixed, paraffin-embedded (FFPE) blocks to assess the expression of relevant biomarkers.

Tetrahydroindazolone-substituted Benzamide Compound W-H4 Induces Apoptosis and Autophagy of Acute Myeloid Leukemia Cells.

Background/aim: Acute myeloid leukemia (AML) is an aggressive hematologic malignancy. Hsp90 inhibitors, like SNX-2112, are promising anti-cancer agents. However, SNX-2112's clinical use is limited by poor solubility and side effects.

Leveraging Behavioral Sciences to Augment Voluntary Blood Donation in China: A Randomized Control Trial and Latent Class Analysis.

Background: China is facing a blood shortage crisis and needs to increase its voluntary blood donation rates. Since current programs to promote blood donation may not be sufficient to meet the rising demand, innovative approaches are needed. Behavioral sciences can provide useful insights for developing behavioral interventions to enhance blood donation.

Coordination Environment Engineering of Titanium Spinel for Enhanced Lithium Recovery.

Salt lake lithium extraction accounts for 35% of global LiCO production, but current adsorbents are limited by single coordination environments. Here, we engineer the coordination environment of titanium spinel (HLiTiO, HTO) by selectively exposing (110) and (111) crystal facets to modulate the tetrahedral and octahedral Li sites. The (111) facet exhibits high adsorption capacity (41.

MicroRNA-29a-5p attenuates hemorrhagic transformation and improves outcomes after mechanical reperfusion for acute ischemic stroke.

Background: Hemorrhage transformation (HT) following endovascular reperfusion treatment is associated with worse clinical outcomes in acute ischemic stroke patients. MicroRNA (miR) modulates several aspects of cerebral ischemia-reperfusion injury, including blood-brain barrier (BBB) integrity, inflammation, oxidative stress, and apoptosis, significantly impacting cerebral recovery and function. This study investigated the role of astrocytic miR-29a-5p in HT in the transient middle cerebral artery occlusion (MCAO) model and oxygen-glucose deprivation reoxygenation (OGD/R) model of astrocytes.

Puerarin suppresses the oxidative stress and activates the cyclic AMP (cAMP)/cAMP response element binding protein/brain-derived neurotrophic factor signaling pathway in alcohol withdrawal-induced depressive disorder via regulating obesity-associated protein-mediated N6-methyladenosine demethylation.

Objective: Puerarin, a bioactive isoflavone glycoside predominantly extracted from the root of the kudzu plant ( Pueraria lobata ), is a traditional Chinese medicinal herb widely used for centuries. Alcohol withdrawal-induced depression (AWIDD), a serious psychiatric disorder, is prevalent in society. This study aimed to investigate the role of puerarin in oxidative stress and cyclic AMP (cAMP)/cAMP response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF) signaling pathway in AWIDD, as well as the underlying mechanism.

Distinguished Loop-Mediated Isothermal Amplification Assay to Detect Porcine Epidemic Diarrhea Virus Genotypes I and II.

Porcine epidemic diarrhea virus (PEDV), a primary pathogen causing diarrhea in pigs, particularly in piglets, has a mortality rate of up to 100%. Field PEDV strains circulating in pig production can be phylogenetically divided into two genotypes, GI and GII. Differential diagnosis of clinical strains with different genotypes is helpful for understanding disease epidemiology, vaccine selection, and prevention and control measures.

Distinct Transcriptomic Profiles of Cultured Anterior and Posterior Populations of Human Infant Scleral Fibroblasts: Including Dopamine Receptors.

Purpose: The transcriptomic profiling of scleral fibroblasts remains largely unexplored. To elucidate their heterogeneity, we performed single-cell RNA sequencing (scRNA-seq) on primary infant scleral fibroblasts.

Methods: Primary scleral fibroblasts, cultured at passage 2 from the anterior, equatorial, and posterior regions of infant sclera (3 months to 2 years of age) were subjected to scRNA-seq using the 10x Genomics platform.

Can Intra-Operative Ablation-Specific Features Based on Ultrasound Fusion Imaging be Used to Predict Early Recurrence of Hepatocellular Carcinoma After Microwave Ablation: A Proof-of-Concept Study.

Purpose: Intra-operative factors are crucial to early recurrence of hepatocellular carcinoma (HCC) after microwave ablation (MWA), but few models have been developed based on intra-operative data to predict HCC recurrence after MWA. To quantify the intra-operative factors associated with MWA and establish an artificial intelligence (AI) model for predicting early recurrence of HCC after ablation based on contrast-enhanced ultrasound (CEUS) fusion imaging.

Patients And Methods: 79 hCC patients, who underwent MWA with one-year follow-up and intraoperative CEUS fusion imaging assessment were retrospectively included.

Ganoderma lucidum extract reduces skin aging by reducing mitochondrial stress and controlling mitochondrial numbers.

Mitochondrial dysfunction is one of the important signs of cellular and even individual aging. Ganoderma lucidum is a common edible and medicinal mushroom, widely used as a functional food in Asia. This study investigated the potential anti-aging effects of Ganoderma lucidum extract (GLE).

Single-cell multiomic analysis unveils the immune landscape dynamics of graves' ophthalmopathy.

Graves' ophthalmopathy (GO) is an autoimmune disease that occurs concurrently with Graves' disease, potentially leading to facial disfigurement and irreversible vision loss. However, the molecular characteristics of cellular changes in the peripheral immune environment of GO patients remain unclear. This study presents a multi-omics single-cell analysis of peripheral blood from GO patients and healthy controls, revealing key molecular alterations in immune cell subpopulations.

Influence of Occult Hepatitis B Infection on Blood Transfusion Safety and Its Countermeasures.

Occult hepatitis B infection (OBI) is a serious public health issue. Although a number of effective hepatitis B vaccines are available, hepatitis B still poses a threat to global public health. Patients with OBI are usually asymptomatic, but there may be active HBV DNA present in their blood, leading to the risk of virus transmission during blood transfusions or organ transplantation, constituting a hazard to the health of recipients and increasing the risk of liver cirrhosis and liver cancer.

Metformin's mechanism in reducing oxidative stress and promoting bone regeneration in T2DM rat BMMSCs: A focus on NRF2-GPX7 signaling pathway.

Objectives: Metformin (MF) could improve the osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) and bone regeneration under diabetes mellitus (T2DM) environment, however, its specific mechanism has not been elucidated. The aim of this study was to investigate whether MF inhibited oxidative stress and promoted osteogenic differentiation of T2DM rats BMMSCs (Tr-BMMSCs) through NRF2-GPX7 pathway.

Methods: BMMSCs were extracted from normal and diabetic rats.

Covalent organic framework membranes for lithium extraction: facilitated ion transport strategies to enhance selectivity.

The surging global demand for lithium, driven by the proliferation of electric vehicles and energy storage technologies, has exposed significant limitations in conventional lithium extraction methods, including inefficiency and environmental harm. Covalent organic frameworks (COFs) have emerged as a promising platform to address this challenge and enable more sustainable lithium extraction, owing to their unique advantages such as precisely tunable pore sizes, robust stability, and the ability to incorporate functional binding sites for selective ion transport. This review focuses on structural design and functionalization strategies in COFs to optimize lithium-ion separation, highlighting how pore confinement effects, tailored interlayer stacking arrangements, and strategic functional group modifications can dramatically enhance Li selectivity over competing ions present in brine solutions.

A Contrast-Enhanced Ultrasound Cine-Based Deep Learning Model for Predicting the Response of Advanced Hepatocellular Carcinoma to Hepatic Arterial Infusion Chemotherapy Combined With Systemic Therapies.

Recently, a hepatic arterial infusion chemotherapy (HAIC)-associated combination therapeutic regimen, comprising HAIC and systemic therapies (molecular targeted therapy plus immunotherapy), referred to as HAIC combination therapy, has demonstrated promising anticancer effects. Identifying individuals who may potentially benefit from HAIC combination therapy could contribute to improved treatment decision-making for patients with advanced hepatocellular carcinoma (HCC). This dual-center study was a retrospective analysis of prospectively collected data with advanced HCC patients who underwent HAIC combination therapy and pretreatment contrast-enhanced ultrasound (CEUS) evaluations from March 2019 to March 2023.

Development and validation of a disulfidptosis-related genes signature for predicting outcomes and immunotherapy in acute myeloid leukemia.

Background: Acute myeloid leukemia (AML) is a hematopoietic malignancy with poor outcomes and high recurrence. Disulfidptosis, a novel form of programmed cell death driven by aberrant disulfide bonds and F-actin collapse, provides insights into cancer progression and treatment.

Methods: We investigated the correlation network and prognostic values of disulfidptosis-related genes (DRGs) in AML.