Functional Study of the BMP Signaling Pathway in Appendage Regeneration of .

Appendage autotomy frequently occurs during the cultivation of , which severely impacts its survival and economic benefits. To investigate the molecular mechanism underlying appendage regeneration in , this study presents a comparative transcriptome analysis on samples from different stages of appendage regeneration in individuals of the same family of . A total of 6460 differentially expressed genes (DEGs) were identified between the samples collected at 0 h post-autotomy (D0) and those collected at 18 h post-autotomy (D18h).

Role of LPC 18:1 in the Lipid Metabolic Response to Combined Vitamin D and Resistance Exercise for Protecting Diabetic Skeletal Muscle Function.

Purpose: We have previously found that vitamin D and resistance exercise synergistically improve type 2 diabetes mellitus (T2DM)-related skeletal muscle atrophy. This study aims to investigate the impact of varying exercise intensity on synergistic effects of vitamin D and resistance exercise on their efficacy in improving type 2 diabetes mellitus (T2DM)-induced myopathy, and further elucidate the underlying mechanism.

Methods: We compared the effects of vitamin D combined with low-, moderate- and high-intensity resistance exercise on metabolic status and skeletal muscle function.

requires unsaturated fatty acids to overcome toxicity of environmental saturated fatty acids.

synthesizes phospholipids from either synthesized or exogenous fatty acids. However, environmental saturated fatty acids are toxic to . The mechanism of toxicity is unknown.

Impact of Multidisciplinary Collaboration on Microbiological Specimen Submission and Appropriate Use of Carbapenems: A Pre-Post Cohort Study.

Purpose: This study assessed the impact of multidisciplinary collaborative management on microbiological specimen submission rates and the rational use of carbapenem antibiotics.

Patients And Methods: A pre- and post-intervention cohort design was employed. From February to November 2024, a multidisciplinary antimicrobial stewardship intervention was implemented for hospitalized patients receiving carbapenem antibiotics.

Transcutaneous auricular vagus nerve stimulation regulates gut microbiota mediated peripheral inflammation and metabolic disorders to suppress depressive-like behaviors in CUMS rats.

Background: Depression is a common mental disorder, and the changes of intestinal microflora and peripheral plasma metabolites can affect the gut-brain axis through vagus nerve, leading to the occurrence, and progress of the disease. Transcutaneous auricular vagus nerve stimulation (taVNS) has been previously shown to be clinically safe and effective in treating depression. However, there is no evidence whether its antidepressant effect is related to the regulation of intestinal flora and metabolites.

Effect of hydrocolloid dressing combined with continuous nursing intervention on patients with permanent ostomy.

Background: This study aimed to retrospectively evaluate the effect of early postoperative hydrocolloid dressing combined with continuous nursing intervention on the quality of life of patients with permanent ostomy.

Methods: This non-randomized retrospective study included 128 patients who underwent permanent ostomy surgery (primarily colostomy) between January 2020 and June 2023. Patients treated after mid-2021 received hydrocolloid dressing and continuous nursing as part of a hospital quality improvement initiative (observation group, n = 64), whereas those treated prior to mid-2021 received standard care (control group, n = 64).

Defining the Enterococcus faecalis Fatty Acid Kinase System of Exogeneous Fatty Acid Utilization.

Phospholipid synthesis in Firmicute bacteria differs markedly from that of the paradigm Escherichia coli pathway in that acyl phosphates are a key intermediate. Acyl phosphates are required for the first acylation step of the phospholipid synthesis pathway catalyzed by the PlsY acyltransferase and are synthesized by two different pathways. In the absence of exogenous fatty acids, de novo synthesized acyl-acyl carrier protein (ACP) species are converted to acyl phosphates by the PlsX acyl-ACP: phosphate acyltransferase, which transfers the acyl chain from ACP to inorganic phosphate.

Acidic pH as the deterministic factor shaping microbial community dynamics and interactions in molasses-fed sulfate-reducing bioreactors.

Sulfate-reducing bacteria (SRB) are widely used in treating high-sulfate wastewater, but their performance is inhibited by low pH conditions. This study investigated prokaryotic community responses to pH decrements from 5.5 to 2.

Global Prevalence of Hypertension in Children and Adolescents Younger Than 19 Years: A Systematic Review and Meta-Analysis.

Importance: Pediatric hypertension predicts adult hypertension and early target organ damage, yet global prevalence estimates based on different diagnostic principles remain scarce.

Objective: To estimate global pediatric hypertension prevalence according to different diagnostic principles.

Data Sources: Several databases, including PubMed, Web of Science, Embase, the China National Knowledge Infrastructure (CNKI), and the Wanfang Database, were systematically searched from inception to July 7, 2024.

The impact of maternal health and lifestyle on low birth weight: a prospective cohort study.

Background: To explore maternal pregestational and periconceptional factors associated with low birth weight in offspring and inform the development of targeted interventions.

Methods: A prospective birth cohort involving 34,104 pregnant women and their offspring was constructed. The participants were enrolled during 8-14 gestational weeks and followed up at 3 months postpartum.

Machine learning-driven prognostic model based on sphingolipid-related gene signature in pancreatic cancer: development and validation.

Background: Pancreatic cancer, a highly malignant tumor with poor prognosis, lacks effective early diagnosis and treatment strategies. Sphingolipids have emerged as key players in tumorigenesis, with certain sphingolipid-related genes linked to patient survival. This study aims to identify prognostic glycosphingolipid (GSL)-related genes and construct a predictive model to improve survival prediction and guide personalized treatment.

Molecular Insights into Diapause Mechanisms in for Improved Biological Control.

This study explores the molecular mechanisms underlying diapause in the parasitoid wasp (Nixon) (Hymenoptera: Platygastridae), a critical egg parasitoid for the biological control of the invasive pest (Smith) (Lepidoptera: Noctuidae). While effective in pest management, faces limitations in large-scale applications due to its short lifespan and low viability under storage conditions. Diapause, a state of developmental arrest, was successfully induced in using photoperiod manipulation (0L:24D), allowing for extended survival and improved storage potential.

Immunotranscriptomic Profiling of Challenged by Different Pathogenic Microorganisms.

is a globally significant migratory agricultural pest that requires proactive monitoring. Understanding the molecular mechanisms underlying the interactions between pathogenic microorganisms and is crucial for enhancing the effectiveness of microbial control agents against this pest. This study used transcriptome sequencing and molecular biology techniques on larvae infected by bacteria and fungi to investigate the composition and molecular regulatory mechanisms of its immune system.

Dynamics of tertiary lymphoid structures and immune cross talk in early versus advanced colorectal cancer: potential implications for immunotherapy.

Background: Irrespective of microsatellite status, immune checkpoint inhibitor therapy shows superior efficacy in early-stage colorectal cancer (CRC) compared to advanced cases. The distinctions of the tumor microenvironment (TME) and tertiary lymphoid structure (TLS) between early- and advanced-stage CRC may represent a critical factor, yet remain incompletely elucidated.

Methods: We comprehensively analyzed single-cell RNA sequencing data, bulk RNA transcription data and pathological tissue data to investigate the dynamic changes in the TME.

A case report on the implantation of a leadless pacemaker in a patient with eosinophilic fasciitis and third-degree atrioventricular block.

Eosinophilic fasciitis (EF) is a rare connective tissue disorder characterized by the involvement of the dermis, subcutaneous tissue, and fascia. The treatment for EF usually involves long-term use of glucocorticoids and immunosuppressants. Patients with EF are at risk of developing third-degree atrioventricular (AV) block during the course of the disease.

Efficient activation of persulfate with bismuth tungstate in dark for degradation of organic pollutants.

Bismuth tungstate (BiWO, BWO) is usually utilized as a photocatalyst for the degradation of various organic pollutants. However, its catalytic potential without light irradiation in persulfate (PS) based advanced oxidation processes (AOPs) has been overlooked, despite its significant redox capacity. Herein, the flower-like orthorhombic BWO was synthesized using a simple hydrothermal method, followed by an evaluation of its catalytic activity in dark through the removal of aqueous organic dye rhodamine B and antibiotic levofloxacin in PS based AOPs.

Summary of Best Evidence for the Dietary Management in Patients with High-Output Ileostomy.

Objective: This study aims to systematically search, screen, evaluate, and summarize the best evidence related to the dietary management of patients with high-output ileostomy, providing an evidence-based reference for the dietary management tailored to this specific demographic.

Methods: A comprehensive search was conducted across multiple databases, including BMJ best practice, Up to Date, Guidelines International Network, Medlive, National Institute for Health and Care Excellence, Scottish Intercollegiate Guidelines Network, Registered Nurses Association of Ontario, JBI, Cochrane Library, PubMed, CNKI, Wanfang, and VIP databases. Additional searches included websites of professional organizations such as the European Society for Clinical Nutrition and Metabolism, Chinese Society for Parenteral and Enteral Nutrition, American Society for Parenteral and Enteral Nutrition, World Council of Enterostomal Therapists, United Ostomy Associations of America, and International Ostomy Association.

Estimated pulse wave velocity is associated with all-cause and cardiovascular mortality in individuals with stroke: A national-based prospective cohort study.

Extensive evidence underscores the potential of estimated pulse wave velocity (ePWV) as a robust tool for predicting disease prevalence and mortality. However, its comparative effectiveness in forecasting all-cause and cardiovascular disease (CVD) mortality, particularly among stroke populations, remains inadequately characterized in relation to the traditional Framingham Risk Score (FRS) model. This prospective study included 1202 individuals with stroke from the National Health and Nutrition Examination Survey conducted between 1999 and 2014, with comprehensive follow-up data.

FOXM1-Driven CKS1B Upregulation Promotes Pancreatic Cancer Progression and Therapeutic Resistance.

Pancreatic ductal adenocarcinoma (PDAC) remains a highly lethal malignancy with limited treatment options. Investigating novel therapeutic targets and understanding mechanisms of chemoresistance are crucial for improving patient outcomes. This study investigated the role of CKS1B in PDAC carcinogenesis, stemness and chemoresistance, and explores the underlying mechanisms driving its upregulation.

Correlation between weight-adjusted waist index and coronary heart disease: NHANES 1999-2020.

Background: The weight adjusted waist index (WWI) represents a novel indicator for assessing central obesity. The objective of this study is to investigate the association between WWI and coronary heart disease (CHD).

Method: The data of 44,528 participants in total were gathered from NHANES database from 1999 to 2020.

Mitochondrial calcium homeostasis and atrial fibrillation: Mechanisms and therapeutic strategies review.

Atrial fibrillation (AF) is tightly linked to mitochondrial dysfunction, calcium (Ca²⁺) imbalance, and oxidative stress. Mitochondrial Ca²⁺ is essential for regulating metabolic enzymes, maintaining the tricarboxylic acid (TCA) cycle, supporting the electron transport chain (ETC), and producing ATP. Additionally, Ca²⁺ modulates oxidative balance by regulating antioxidant enzymes and reactive oxygen species (ROS) clearance.

OSBPL3 modulates the immunosuppressive microenvironment and predicts therapeutic outcomes in pancreatic cancer.

Background: Pancreatic cancer is characterized by a complex tumor microenvironment that hinders effective immunotherapy. Identifying key factors that regulate the immunosuppressive landscape is crucial for improving treatment strategies.

Methods: We constructed a prognostic and risk assessment model for pancreatic cancer using 101 machine learning algorithms, identifying OSBPL3 as a key gene associated with disease progression and prognosis.

The regulatory module MdCPCL-MdILR3L mediates the synthesis of ascorbic acid and anthocyanin in apple.

Apple (Malus domestica Borkh.) is one of the most economically valuable fruit crops globally and a key dietary source for various nutrients. However, the levels of ascorbic acid (AsA) and anthocyanin, essential micronutrients for human health, are extremely low in the pulp of commonly cultivated apple varieties.

FOXM1-Cx31 Axis Drives Pancreatic Cancer Stem Cell-Like Properties and Chemoresistance.

Pancreatic cancer is a highly lethal malignancy with few effective treatment options. Connexin 31 (Cx31) is a membrane protein capable of forming hexameric channels to facilitate the exchange of metabolites and signaling molecules. Yet, the contribution of Cx31 to the onset and progression of pancreatic cancer remains to be understood.

stMMR: accurate and robust spatial domain identification from spatially resolved transcriptomics with multimodal feature representation.

Background: Deciphering spatial domains using spatially resolved transcriptomics (SRT) is of great value for characterizing and understanding tissue architecture. However, the inherent heterogeneity and varying spatial resolutions present challenges in the joint analysis of multimodal SRT data.

Results: We introduce a multimodal geometric deep learning method, named stMMR, to effectively integrate gene expression, spatial location, and histological information for accurate identifying spatial domains from SRT data.