DNA methylation of the ABCA1 and ABCG1 genes contribute to stroke risk.

Introduction: Stroke is a major cause of death in China. The epigenetic factors, especially the methylation of ABCA1/G1 genes implicated in cholesterol regulation, are being examined to comprehend their association with stroke.

Methods: In this nested case-control study, we examined data from the North-west China Cohort (CNC-NX) initial phase, involving 53 pairs of stroke cases and controls.

Comparative Genomic Analysis of Tigecycline Resistance Development in Clinical Isolates.

Objective: This study investigated the mechanisms underlying the reduced tigecycline sensitivity of strains isolated from infected patients during antimicrobial therapy. The goal is to provide clinical insights into the development of tigecycline resistance in , with a focus on multidrug-resistant strains commonly associated with hospital-acquired infections.

Methods: We conducted dynamic tracking of multidrug-resistant (MDR-AB) infections in three patients to monitor changes in tigecycline sensitivity during antimicrobial therapy.

Modulating Lewis acidity by oxygen vacancies engineering in Ce-doped manganese dioxides to promote permanganate activation.

The development of efficient and eco-friendly solid catalysts with strong Lewis acid sites for permanganate (KMnO) activation is highly desired for pollutant removal. In this study, the oxygen vacancy (O) content of cryptomelane-type manganese oxide, was modulated by Ce doping to promote its Lewis acidity. The findings show that incorporating Ce into the framework of MnO octahedra inhibits the growth of the catalyst to form a completely amorphous structure, with the increase of O ratio, especially bulk O content.

Effect of butyryl substitution degree in cellulose acetate butyrate (CAB) on A3 plasticizer migration: Mechanism analysis.

Plasticizer migration is a significant issue in the utilization of polymer materials. For cellulose derivatives substituted with two or more different groups, such as cellulose acetate butyrate (CAB), the varying proportions of acetyl and butyryl groups lead to different chemical structures, which influence the plasticizer migration properties. This paper investigates the effects of the butyryl substitution degree on plasticizer migration properties through experimental testing and molecular dynamics simulations.

Mitochondrial Regulation of Spermatozoa Function: Metabolism, Oxidative Stress and Therapeutic Insights.

Mitochondria are central to energy production and redox regulation in spermatozoa, supporting key functions such as progressive motility, capacitation, and the acrosome reaction. These processes are essential for successful fertilization and embryo development. However, species-specific differences exist in the reliance on oxidative phosphorylation versus glycolysis.

An investigation into the impact of family integrated care on extrauterine growth restriction at discharge in very low birth weight infants: a multi-centre study.

Background: Family integrated care (FIC) encourages parental involvement in neonatal intensive care units (NICU) and has been found to promote weight gain in preterm infants. Extrauterine growth restriction (EUGR) results from inadequate growth among very low birth weight infants (VLBWI), which has been found to contribute to parental anxiety. To address an existing gap in research, we aimed to examine the impact of parental involvement on EUGR at discharge in VLBWI.

Percutaneous endoscopy in the management of intra-abdominal complex disorders.

Background And Aims: Intra-abdominal complex disorders pose significant challenges to clinicians due to their intricate nature and diagnostic uncertainties. This study aims to comprehensively evaluate the feasibility, safety, and efficacy of percutaneous endoscopy in the management of such conditions.

Methods: A retrospective analysis was conducted on the clinical data of 37 patients diagnosed with intra-abdominal complex disorders who underwent percutaneous endoscopic examination and treatment at Mianyang 404 Hospital in Sichuan, China, between January 2016 and October 2024.

Healing and Skeletal Development Outcomes of a Complex Case Involving Complicated Crown-Root Fracture and Luxation Injuries With a 4-Year Follow-Up.

The management of complicated crown-root fractures of immature permanent teeth requires a multidisciplinary approach to address biological, functional, and aesthetic concerns. A 10-year-old boy suffered a complicated crown-root fracture of tooth 11, an uncomplicated crown fracture, subluxation and pulp necrosis of tooth 21 and subluxation of tooth 22 after falling from a bicycle. The treatment for tooth 11 involved maintaining pulp vitality with partial pulpotomy and re-attachment of the crown-root fragment after modified crown-lengthening surgery.

MXene/Carboxymethyl Chitosan Moisture Responsive Soft Actuator with Diode-Like Actuation for Versatile Applications Driven by Human Metabolism.

Moisture responsive soft actuators are receiving increasing attention due to their unique potential in reducing external energy dependence and carbon footprint. For the conventional moisture responsive soft actuators, their bending deformation under moisture stimulation is usually bidirectional, and the orientation of the bending axis is random. Achieving a moisture responsive monolithic actuator with controllable unidirectional deformation remains a challenge.

Accurate Neurotransmitter Release by Ultrasound-Responsive Nanomaterials for Alleviating L-DOPA-Induced Dyskinesia in Parkinson's Disease.

Functional nanomaterials show great promise in next-generation targeted non-genetic neuromodulation and on-demand neurotransmitter release with superior spatiotemporal accuracy. L-DOPA-induced dyskinesia (LID) is a common complication during the treatment process of Parkinson's disease. Dysfunction in the dynamics of dopamine release plays a pivotal role in the disease.

Red-emissive carbon dot-cobalt oxyhydroxide nanosystem: A turn-on sensor for α-Glucosidase activity and inhibitor identification.

The development of efficient methods for sensing αlpha-glucosidase (α-Glu) and screening its inhibitors has attracted significant attention due to their pivotal role in discovering therapeutic medicines for Type 2 diabetes. Herein, a low-cost and sensitive fluorometric strategy based on red carbon dots (R-CDs) and cobalt oxyhydroxide nanosheets (CoOOH NSs) had been established to detect α-Glu and screen its inhibitory compounds in natural products. As a switched fluorescence source, the fluorescence of R-CDs at 625 nm could be quenched by CoOOH NSs via Förster resonance energy transfer (FRET), assembled into nonfluorescent R-CDs@CoOOH nanocompositecomposites (R-CDs@CoOOH NCs).

Mechanism of Neutrophil p90RSK-Nrf2 Signaling Pathway in Atherosclerosis.

Background: MRP8/14, a calcium-binding protein of the S100 family, is predominantly expressed in myeloid cells and exhibits proinflammatory and prothrombotic properties. Platelet-neutrophil interactions can trigger MRP8/14 release, but their role in atherosclerosis (AS) remains unclear.

Aims: To investigate the effect of MRP8/14 on AS progression and the underlying mechanisms involved, focusing on neutrophil activation and the toll-like receptor 4 (TLR4)-ERK1/2-p90RSK and NRF2-ARE pathways.

A robust resilient-oriented design for the cyber-physical distribution system against sequential typhoons.

The cyber-physical deep coupling makes distribution systems face severe operation risks under small-probability and high-impact disasters. To enhance the resilience of cyber-physical distribution systems against typhoons, this paper proposes a resilience-oriented robust optimization model, in which planning-operational restoration measures are incorporated into a prevention and emergency response framework. In the prevention response stage, line hardening, battery storage systems and soft open point deployment, as well as wireless communication configuration are conducted before typhoons.

A computer-assisted design of Poly(deep eutectic solvent)@MIL-101-NH(Cr) imprinting strategy: Selective removal of zearalenone from coix seeds.

Zearalenone (ZEN), a common mycotoxin, is prevalent in food and agricultural by-products, presenting considerable health risks. A novel strategy was established in this research based on the computer-aided design synthesis of Poly(DES)@MIL-101-NH(Cr) for effective removal of ZEN. Initially, the optimal ratio of template molecules to deep eutectic solvents (DES) was predicted using density functional theory (DFT), and Poly(DES)@MIL-101-NH(Cr) was synthesized through surface imprinting technology.

Simultaneous double primary malignant tumors of MSS/pMMR ascending colon cancer and MSI-H/dMMR duodenal cancer with nearly 2 years of recurrence-free survival after MDT-guided comprehensive treatment: a case report.

The exceedingly rare clinical presentation of synchronous ascending colon carcinoma with duodenal adenocarcinoma demonstrating discordant mismatch repair protein expression patterns forms the cornerstone of this investigation. Through detailed analysis of a unique case featuring co-occurring MSS/pMMR ascending colon adenocarcinoma and MSI-H/dMMR duodenal adenocarcinoma, this study demonstrates the successful implementation of multidisciplinary therapeutic protocols achieving 22-month progression-free survival post-radical resection. These clinical findings offer empirical evidence for optimizing clinical management while systematically addressing diagnostic complexities and treatment dilemmas inherent to synchronous dual primary intestinal carcinomas.

Regulatory role of circRNAs in mammalian pregnancy.

Circular RNAs (circRNAs) have emerged as key regulators in mammalian pregnancy, influencing crucial processes such as placental development, immune modulation, and hormonal regulation. Their unique covalently closed-loop structure confers exceptional stability, making them ideal candidates as non-invasive biomarkers for monitoring pregnancy progression and diagnosing complications. This review explores the physiological roles of circRNAs during pregnancy, highlighting their involvement in trophoblast invasion, angiogenesis, and maternal-fetal communication.

Kinetic analysis of thermally induced polymorphic transition in HMX using terahertz spectroscopy.

Understanding the kinetics of polymorphic transitions is critical for predicting and controlling phase behavior in functional materials. Although terahertz spectroscopy has been established as a reliable technique for identifying polymorphic forms, its application in analyzing transition kinetics remains underexplored. In this study, terahertz time-domain spectroscopy (THz-TDS) was employed to monitor the thermally induced β-to-δ polymorphic transition in HMX.

Low-Dose Methotrexate for the Treatment of Inflammatory Knee Osteoarthritis: A Randomized Clinical Trial.

Importance: A recent study reported that methotrexate may reduce joint pain in patients with inflammatory hand osteoarthritis (OA). However, it remains unknown whether methotrexate has similar effects on inflammatory knee OA.

Objective: To examine whether methotrexate has symptom-relieving and disease-modifying effects for participants with knee OA and effusion-synovitis.

Pluripotent stem cell-derived cardiomyocyte transplantation: marching from bench to bedside.

Cardiovascular diseases such as myocardial infarction, heart failure, and cardiomyopathy, persist as a leading global cause of death. Current treatment options have inherent limitations, particularly in terms of cardiac regeneration due to the limited regenerative capacity of adult human hearts. The transplantation of pluripotent stem cell-derived cardiomyocytes (PSC-CMs) has emerged as a promising and potential solution to address this challenge.

Characterizing pancytopenia in a large Asian cohort of people living with HIV: A 23-year nested case-control study.

Background: Pancytopenia, a severe hematologic comorbidity, raises significant clinical concern for increased morbidity and mortality in people living with HIV (PLWH). However, reports on its incidence, risk factors, real-world etiologies, longitudinal characteristics, or management strategies in PLWH are absent. Through a long-term study of a large Asian cohort, this work aims to provide insights into these aspects.

Oat β-Glucan and Lacticaseibacillus paracasei K56 synergistically ameliorate hypercholesterolemia in mice by modulating gut microbiota and bile acid metabolism.

Hypercholesterolemia is one of the major risk factors for cardiovascular diseases. While probiotics and prebiotics show cholesterol-lowering potential, current evidence suggests limited efficacy when used alone. This study investigated the synergistic effects of oat β-glucan (OBG) and Lacticaseibacillus paracasei K56 (L.

Factors influencing pathological response after neoadjuvant therapy for advanced gastric cancer.

Objective: To identify the factors influencing pathological responses after neoadjuvant therapy in advanced gastric cancer and to construct an effective prediction model for an improved response.

Methods: Clinical data from 100 patients with advanced gastric cancer who received neoadjuvant therapy at The Fifth Hospital of Wuhan from January 2020 to December 2023 were retrospectively analyzed. Basic data, laboratory test results, and other patient information were collected.

A CT-based intratumoral and peritumoral radiomics nomogram for postoperative recurrence risk stratification in localized clear cell renal cell carcinoma.

Objectives: This study aimed to develop and validate a computed tomography (CT)-based intratumoral and peritumoral radiomics nomogram to improve the stratification of postoperative recurrence risk in patients with localized clear cell renal cell carcinoma (ccRCC).

Methods: This two-center study included 447 patients with localized ccRCC. Patients from Center A were randomly split into a training set (n = 281) and an internal validation set (IVS) (n = 114) in a 7:3 ratio, while 52 patients from Center B formed the external validation set (EVS).

Zinc finger protein 750 is a novel regulator of osteoblast differentiation and bone homeostasis by transcriptionally deactivating SNAI1 signaling.

Zinc finger protein 750 (ZNF750) has been identified as a potential tumor suppressor across multiple malignancies. Nevertheless, the specific involvement of ZNF750 in the regulation of mesenchymal cell differentiation and bone homeostasis has yet to be elucidated. In the current study, we observed a substantial presence of ZNF750 in bone tissue and noted alterations in its expression during osteogenic differentiation of mesenchymal progenitor cells.

Fenofibrate inhibits activation of cGAS-STING pathway by alleviating mitochondrial damage to attenuate inflammatory response in diabetic dry eye.

The cyclic GMP-AMP synthase (cGAS) and Stimulator of Interferon Genes (STING) signaling pathway are critical regulators of inflammation. This study aims to investigate the role of the cGAS-STING signaling pathway in diabetic dry eye (DDE) disease and further explore the therapeutic efficacy and underlying mechanism of fenofibrate in DDE. Using single-cell RNA sequencing (scRNA-Seq) data from the Gene Expression Omnibus (GEO) database, combined with the STZ-induced DDE mouse model and high-glucose conditions in immortalized human corneal epithelial cells (HCE-T), we observed mitochondrial damage and significantly elevated cytoplasmic mitochondrial DNA (mtDNA) in the diabetic cornea, and identified that the cGAS-STING signaling pathway plays a pivotal role in the pathogenesis of DDE.