Hollow Double-Layered Mesoporous Silica as an Enzyme Immobilization Platform: Enhancing Biocatalytic Efficiency in Docosahexaenoic Acid Phosphatidylcholine-Phosphatidylcholine.

This study reports the synthesis of bifunctional hollow double-layered mesoporous silica (HdlMS) nanoparticles as an effective support for enzyme catalysis. Using a self-template method combined with sol-gel processing, selective etching, and surface modification with alkyl groups and nitrogenous fatty amine groups, we successfully prepared the NH/C-HdlMS carrier. Phospholipase A1 was then immobilized onto this carrier to construct PLA1@NH/C-HdlMS, which was subsequently applied to the synthesis of docosahexaenoic-acid-enriched phosphatidylcholine.

1+1>2: Synergistic Integration and Biomedical Applications of Nanozyme@Hydrogel Platforms.

Nanozyme-based therapies have shown substantial promise in antibacterial, anti-inflammatory, and anticancer applications. The success of nanozymes in these areas largely depends on strategies such as chemical modification, ligand coupling, or carrier encapsulation, which enhance their in vivo stability and catalytic activity. Hydrogels, characterized by their soft, water-swollen three-dimensional (3D) networks, possess unique features such as biodegradability, tunable physicochemical properties, and injectability.

Effect of continuity of care on self-management, quality of life, and clinical outcomes in patients with chronic heart failure.

This research aims to investigate the impact of continuity of care on individuals diagnosed with chronic heart failure (CHF), specifically examining its effects on their self-care capabilities, life quality, mental well-being, and physical condition. A total of 90 patients diagnosed with CHF and treated at our hospital between January and September 2023 were included in this retrospective controlled study. Propensity score matching was used to assign patients into 2 comparable groups: an intervention group and a control group (CG), each consisting of 45 individuals.

Long-Term In Situ Treatment of Arthritis with Injectable Biocompatible Organogel Activation.

Rheumatoid arthritis (RA), an autoimmune disease, severely impairs joint function and mobility, causing pain, stiffness, and joint deformity. As an active ingredient of ancient herbs and a widely studied polyphenol flavonoid glycoside, quercetin has a historical use in the treatment of rheumatoid arthritis. However, its low oral bioavailability and potential carcinogenic effects limit its systemic use.

Tungsten sulfide sheets with tunable layered structures: Piezocatalysis via multi-channels to generate HO.

Driven by the urgent need for eco-friendly, safe and facile production processes for hydrogen peroxide (HO), piezocatalysis shows significant potential due to its accessible energy supply and capacity for on-site HO preparation. Owing to their tunable surface properties and unique electronic structures, transition metal dichalcogenides (TMDCs) are regarded as promising piezocatalysts. Nevertheless, the uneven distribution of active sites, poor stability, and restricted electron transport capabilities hinder the further improvement of their piezocatalytic performance.

Different concentrations of exopolysaccharides enhance the freeze resistance of Lactobacillus plantarum: Transcriptomics and metabolomics.

The study aimed to investigate the regulatory effects of exopolysaccharides (EPS) derived from Pediococcus acidilactici S1 on the cell physiological functions of Lactobacillus plantarum FQR during the freezing process. Results demonstrated that the addition of 70 mg/mL EPS as a cryoprotectant achieved a maximum survival rate of 91.99 ± 3.

Air pollution increases gastroesophageal reflux disease risk: evidence from a prospective cohort study.

Background: Gastroesophageal reflux disease (GERD) is one of the most prevalent gastrointestinal disorders with uncertain etiology and high prevalence. Ambient air pollution has been linked to gastrointestinal diseases, but the impact of long-term air pollution exposure on GERD incidence is still unclear.

Methods: We performed a cohort study using the UK Biobank database.

Knowledge-aware Synergistic Discovery of Drug Combinations: A Large Language Model Perspective.

Drug combination therapy with significant advantages is a well-established concept in cancer treatment. Some related efforts have been made with multiple artful deep learning techniques. However, they are usually based on data for drug synergy prediction, ignoring the professional characteristics of data and the systematic knowledge accumulation.

An investigation into the impact of family integrated care on extrauterine growth restriction at discharge in very low birth weight infants: a multi-centre study.

Background: Family integrated care (FIC) encourages parental involvement in neonatal intensive care units (NICU) and has been found to promote weight gain in preterm infants. Extrauterine growth restriction (EUGR) results from inadequate growth among very low birth weight infants (VLBWI), which has been found to contribute to parental anxiety. To address an existing gap in research, we aimed to examine the impact of parental involvement on EUGR at discharge in VLBWI.

Polyphyllin H inhibits malignant progression of ovarian cancer in patient-derived xenograft mouse models by regulating CGN/RhoA/Rock2 axis: an experimental research.

Background: Discovering new and effective drugs is a top priority for treating ovarian cancer.,a gynecological tumor with a high mortality rate. As a major active ingredient isolated from Paris polyphylla, Polyphyllin H exhibits antitumor effect.

Genetically predicted ankylosing spondylitis increases the risk of heart failure: a Mendelian randomization study.

Background: Observational studies have indicated a potential association between ankylosing spondylitis and elevated cardiovascular disease (CVD) risk. However, it remains uncertain whether this association reflects a causal association. Therefore, we conducted this Mendelian randomization to investigate the causal relationship between ankylosing spondylitis and CVD.

Role of AQP1 in the Ameliorating Effect of Electroacupuncture in a Rodent Model of Visceral Hypersensitivity.

Background: Aquaporins 1 (AQP1), the first water channel protein, is involved in central and peripheral nociception. However, it remains unclear whether AQP1 contributes to visceral hypersensitivity. We hypothesize that AQP1 contributes to acetic acid-induced visceral hypersensitivity in a rat model of irritable bowel syndrome (IBS) and that electroacupuncture (EA) improves visceral hypersensitivity by down-regulating the expression of AQP1 in enteric glial cells (EGCs) and dorsal root ganglion (DRG).

Survival Disparities by Sex, Race, and Age in the Era of Contemporary Advanced Urothelial Carcinoma Therapy: A Real-World Analysis.

Introduction: Retrospective data suggest poorer survival for female, racial minority, and older advanced urothelial carcinoma (aUC) patients. However, data on survival disparities in the modern era remain limited.

Methods: This cohort study used Flatiron Health's nationwide de-identified electronic health record (EHR)-derived database.

Quercetin combined with shTERT induces apoptosis in ovarian cancer via the P53/Bax pathway, and RGD-MSN/QR/shTERT nanoparticles enhance the therapeutic efficacy.

Background: Ovarian cancer (OC) is a highly malignant gynecological tumor with poor current treatment effects. Telomerase reverse transcriptase (TERT) is an important component of telomerase and plays an important role in the progression of ovarian cancer. Quercetin(QR) has been shown to inhibit the cell cycle and induce the apoptosis in various types of tumors.

The effect and mechanism of sublethal injury by lactic acid on Salmonella Typhimurium planktonic and biofilm cells.

Salmonella is a zoonotic pathogen present in the food and environment, which could survive as sublethally injured form during treatment. The effects of sublethal injury by lactic acid (LA) on S. Typhimurium planktonic and biofilm cells in broth, food manufacturing wastewater, and aqueous solution were investigated in this study.

Flexible Microneedle Platform for Synergistic Chemo/Photothermal Ferroptosis Therapy via P-Glycoprotein Inhibition in Breast Cancer.

Combining photothermal therapy (PTT) with chemotherapy shows promise for cancer treatment, but its efficacy is limited by heat shock protein (HSP)-induced thermoresistance and P-glycoprotein (P-gp)-mediated drug efflux. To overcome these challenges, we developed a tumor microenvironment-responsive microneedle platform for synergistic chemotherapy, PTT, and ferroptosis in breast cancer. The system employs Fe-coordinated dihydromyricetin (DMY) nanocarriers encapsulating doxorubicin (DMFD NPs) integrated into a flexible polyvinylpyrrolidone (PVP) microneedle patch.

A leucine derivate-adjuvanted LNP vaccine enhances antitumor immunity through mTOR activation and metabolic reprograming in dendritic cells.

Neoantigen-derived peptide vaccines have been emergingly employed to treat tumors, however, their inefficient delivery and antigenicity remain challenging. In this study, for the first time a leucine derivate LLOMe (L-leucyl-l-leucine methyl ester) was repurposed to the adjuvant to reinforce the immune responses of lipid nanoparticle (LNP)-based peptide vaccine. Mechanistically, LLOMe disrupted the lysosomal entrapment of vaccine thus effectively enhanced bioavailability of antigens.

Neddylation E1 Obligatory Subunit Nae1 Is Critical to Neuromuscular Junction Development and Maintenance.

Rapsyn is a scaffold protein that is thought to anchor acetylcholine receptors at the neuromuscular junction (NMJ). We showed that it may be an E3 ligase that regulates NMJ development by neddylation. To obtain genetical evidence, we mutated Nae1 (APP-BP1), an obligatory subunit of the neddylation E1 enzyme specifically in muscle cells.

Association between serum osmolality and chronic kidney disease prevalence: Evidence from NHANES 1999-2018.

Background: Chronic kidney disease (CKD) is a significant global public health issue, and early identification of risk factors is critical for prevention and intervention. In recent years, serum osmolality has attracted attention as a potential biomarker; however, its association with CKD remains insufficiently understood.

Methods: This study analyzed data from the National Health and Nutrition Examination Survey (NHANES) conducted between 1999 and 2018, including 49,008 participants.

Copper peroxide-triggered reactive nitrogen effectively enhanced synergistic chemodynamic-immunotherapy.

Overcoming the limitations of chemodynamic therapy (CDT), constrained by the transient nature and limited bioavailability of reactive oxygen species (ROS), this study proposes a synergistic strategy integrating CDT with immunotherapy. We engineered a multifunctional nanocarrier, HMSN-Met@HA-CuO₂, leveraging CuO to amplify intracellular ROS generation and induce cuproptosis. Concurrently, metformin (Met) releases nitric oxide (NO), which scavenges ROS to generate more stable reactive nitrogen species (RNS).

Low expression of SGCA promotes lung squamous cell carcinoma malignant progression.

Lung squamous cell carcinoma (LUSC) is a common type of non-small-cell lung cancer with high mortality, lacks effective diagnostic indicators and therapeutic targets. This study aims to reveal that low expression of Alpha-Sarcoglycan (SGCA) promotes LUSC progression and to provide a biomarker for its diagnosis, prognosis evaluation, and targeted therapy. TCGA data and 70 clinical LUSC patients were analyzed.

A ferritin-targeted biohybrid triggering ferroptosis immunotherapy via activating endogenous iron and replenishing exogenous iron simultaneously.

The key to achieving synergistic ferroptosis immunotherapy is enhancing the iron content in tumor cells, improving specific immunity, and regulating the tumor microenvironment. In this study, a drug-free biohybrid system targeting ferritin is developed using M1 macrophage microvesicles and HKN-modified Prussian blue nanoparticles for synergistic ferroptosis immunotherapy. HKN-modified nanoparticles simultaneously enhance iron content by activating endogenous iron ions and replenishing exogenous iron ions, which disrupts iron homeostasis for inducing ferroptosis in tumor cells.

Cas12a Cis-cleavage mediated lateral flow assay enables multiplex and ultra-specific nucleic acid detection.

CRISPR technology holds significant promise for advancing nucleic acid assays. However, current CRISPR diagnostic techniques, reliant on indiscriminate trans-cleavage mechanisms, face challenges in developing multiplex detection formats. Moreover, chaotic trans-cleavage activity often results from mismatched targets, leading to specificity issues.

Simultaneous inhibition of heterocyclic aromatic amines, advanced glycation end products, acrylamide, and hydroxymethylfurfural in Chinese braised beef by characteristic spices during braising and in vitro digestion.

This research examined how various spices inhibit formation and digestion of free and bound heterocyclic aromatic amines (HAAs), advanced glycation end products (AGEs), acrylamide (AA), and hydroxymethylfurfural (HMF) in Chinese braised beef. Lemongrass reduced total levels of free and bound HAAs, AGEs, HMF and AA in Chinese braised beef, model system, and in vitro digestion (P < 0.05), and the inhibition rates were 67.

siRNA micelleplexes-mediated glutamine metabolism re-engineering for vascular normalization-boosted photo-immunotherapy.

Among tumor microenvironment (TME), the entire metabolic characteristics of tumor-resident cells are reprogrammed to benefit the expansion of tumor cells, which count on glutamine in large part to fuel the tricarboxylic acid cycle for energy generation and anabolic metabolism support. Endothelial cells that are abducted by tumor cells to form a pathological tumor vascular network for constructing the hypoxic immunosuppressive TME, also rely on glutaminolysis as the "engine" of angiogenesis. Additionally, the glutamine metabolic preference benefits the polarization of TAMs towards pro-tumoral M2 phenotype as well.