Screening of an FDA-Approved Drug Library: Menadione Induces Multiple Forms of Programmed Cell Death in Colorectal Cancer Cells via MAPK8 Cascades.

: Colorectal cancer (CRC) is a prevalent gastrointestinal malignancy, ranking third in incidence and second in cancer-related mortality. Despite therapeutic advances, challenges such as chemotherapy toxicity and drug resistance persist. Thus, there is an urgent need for novel CRC treatments.

Molecular Identification of Co-infection with ' Phytoplasma' and Velarivirus 1 in Areca Palms on Hainan Island, China.

Areca palm yellow leaf phytoplasma (AYLP) and Areca palm velarivirus 1 (APV1) are two critical pathogens associated with yellow leaf in areca palm, resulting in devastating damage to areca production. However, evidence of their co-infection remains unclear. Areca palms showing yellow leaf symptoms (suspected to be caused by phytoplasma and velarivirus 1) were surveyed and sampled around Hainan Island of China and the pathogens were identified and analyzed in the study.

Specific Nested PCR for the Detection of 16SrI and 16SrII Group Phytoplasmas Associated with Yellow Leaf Disease of Areca Palm in Hainan, China.

Yellow leaf disease (YLD), caused by the areca palm yellow leaf phytoplasma (APYL), poses a significant threat to the sustainability of the areca palm industry. Timely and accurate detection is essential for effectively diagnosing and managing this disease. This study developed a novel nested PCR system using primers specifically designed from conserved regions of the phytoplasma 16S rDNA sequence to overcome limitations such as false positives often associated with universal nested PCR primers.

Quercetin combined with shTERT induces apoptosis in ovarian cancer via the P53/Bax pathway, and RGD-MSN/QR/shTERT nanoparticles enhance the therapeutic efficacy.

Background: Ovarian cancer (OC) is a highly malignant gynecological tumor with poor current treatment effects. Telomerase reverse transcriptase (TERT) is an important component of telomerase and plays an important role in the progression of ovarian cancer. Quercetin(QR) has been shown to inhibit the cell cycle and induce the apoptosis in various types of tumors.

Physiological Responses and Histopathological Changes in Narrow-Clawed Crayfish () Under Acute Thermal Stress.

To investigate thermal tolerance, physiological responses, and molecular mechanisms of the narrow-clawed crayfish () under acute thermal stress, the were acutely exposed to 4 different temperature groups-15 °C (control), 20 °C (T20), 25 °C (T25), and 30 °C (T30)-across 6 time points (3 h, 6 h, 12 h, 24 h, 48 h, and 72 h). Survival rates were recorded at each interval. Subsequent analyses comprised: (1) Hemolymph biochemical parameter determination; (2) hepatopancreatic antioxidant capacity assessment; (3) hepatopancreatic histopathology; and (4) comparative transcriptomics analysis of the hepatopancreas.

Laser SLAM Matching Localization Method for Subway Tunnel Point Clouds.

When facing geometrically similar environments such as subway tunnels, Scan-Map registration is highly dependent on the correct initial value of the pose, otherwise mismatching is prone to occur, which limits the application of SLAM (Simultaneous Localization and Mapping) in tunnels. We propose a novel coarse-to-fine registration strategy that includes geometric feature extraction and a keyframe-based pose optimization model. The method involves initial feature point set acquisition through point distance calculations, followed by the extraction of line and plane features, and convex hull features based on the normal vector's change rate.

Promotes Pancreatic Cancer Metastasis by Inhibiting to Activate the TGF-β Pathway.

Background: Pancreatic adenocarcinoma (PAAD) represents a highly fatal form of cancer. The 5-year survival rate for patients with this disease is only around 10%. A significant hurdle in its management is the absence of characteristic early-stage symptoms.

Cytogenetics and genomics analysis of cold-hardy perennial wheatgrass: insights into agronomic performance, chromosome composition, and gene expression.

Intermedium wheatgrass (Thinopyrum intermedium), a perennial species with extensive root systems and high tolerance to cold, drought, and salinity, is a valuable genetic resource for the development of perennial crops. Over a decade-long selection process, two cold-hardy perennial wheatgrass lines were developed by crossing wheat-Thinopyrum partial amphiploids with Th. intermedium.

Early-stage lung cancer detection via thin-section low-dose CT reconstruction combined with AI in non-high risk populations: a large-scale real-world retrospective cohort study.

Background: Current lung cancer screening guidelines recommend annual low-dose computed tomography (LDCT) for high-risk individuals. However, the effectiveness of LDCT in non-high-risk individuals remains inadequately explored. With the incidence of lung cancer steadily increasing among non-high-risk individuals, this study aims to assess the risk of lung cancer in non-high-risk individuals and evaluate the potential of thin-section LDCT reconstruction combined with artificial intelligence (LDCT-TRAI) as a screening tool.

Icariside II induces ferroptosis through the down-regulation of SLC7A11 in ovarian cancer.

Background: Ovarian cancer (OV) is the leading cause of death among gynecological malignancies. This study aimed to investigate the influence of Icariside II on OV in vitro and in vivo and to elucidate whether Icariside II induces ferroptosis in OV cells by regulating SLC7A11 expression.

Methods: SKOV3 cells and OV nude mice were treated with Icariside II, a control-plasmid or an SLC7A11-plasmid.

FK506 activates the BMP signaling pathway to regulate ovarian development in Portunus trituberculatus.

Bone morphogenic proteins (BMPs) play important regulatory roles in the development of follicles in mammals. However, studies on the roles of BMPs in ovarian development in low-level aquatic animals, especially the swimming crab Portunus trituberculatus, are limited. In this study, a BMP Ⅰ-type receptor-specific activator (tacrolimus, FK506) was administered at different concentrations via in vivo injection, and the effects of FK506 on the regulation of the BMP signaling pathway during ovarian development in P.

Utilization of Crab Shell Waste for Value-Added Bioplastics by -Based Microbial Cell Factories.

With the development of the aquatic products processing industry, 6-8 million tons of shrimp and crab shell waste are produced globally annually, but, due to the lack of high-value conversion technology, crab shells are often discarded in large quantities as a by-product of processing. -based microbial cell factories are capable of biosynthesis of high-value products using a wide range of substrates; however, there is currently no reliable fermentation model for producing high-value chemicals using crab shell waste by strains. In this study, we first explored the culture conditions of shell fermentation using KT2440 through single-factor and orthogonal experiments, and the optimized fermentation parameters obtained are given as follows: a temperature of 30 °C, fermentation time of 42 h, substrate solid-liquid ratio of 7%, and rotational speed of 200 rpm.

Low levels of CD3 + and CD8 + T cells in peripheral blood can predict poor efficacy of first-line chemotherapy in patients with angioimmunoblastic T cell lymphoma.

To investigate the prognostic significance of lymphocyte subsets in newly diagnosed angioimmunoblastic T-cell lymphoma (AITL) patients and track the dynamic changes in these subsets during treatment. We retrospectively analyzed the medical records in 56 patients with AITL. Lymphocyte subsets were assessed by flow cytometry at diagnosis.

Evaluation of nitrogen removal performance and metabolic mechanism of a novel salt-tolerant strain Pseudomonas aeruginosa SH3.

High salinity impedes efficient nitrogen removal from mariculture wastewater, which inhibits the colonization and nitrogen removal capabilities of nitrogen-removing microbes. This study aimed to isolate and characterize a salt-tolerant heterotrophic nitrification-aerobic denitrification bacterial strain. We evaluated 30 bacterial strains isolated from Portunus trituberculatus aquaculture ponds, among which Pseudomonas aeruginosa SH3 exhibited superior nitrogen removal efficiencies (99 % of NH-N, 71 % of NO-N, and 85 % fof NO-N at a salinity of 30 ‰) than the other strains.

Identification of glycolysis-related gene signatures for prognosis and therapeutic targeting in idiopathic pulmonary fibrosis.

Background: Glycolysis plays a crucial role in fibrosis, but the specific genes involved in glycolysis in idiopathic pulmonary fibrosis (IPF) are not well understood.

Methods: Three IPF gene expression datasets were obtained from the Gene Expression Omnibus (GEO), while glycolysis-related genes were retrieved from the Molecular Signatures Database (MsigDB). Differentially expressed glycolysis-related genes (DEGRGs) were identified using the "limma" R package.

Exploring FAM13A-N-Myc interactions to uncover potential targets in MYCN-amplified neuroblastoma: a study of protein interactions and molecular dynamics simulations.

Neuroblastoma (NB), a common infantile neuroendocrine tumor, presents a substantial therapeutic challenge when MYCN is amplified. Given that the protein structure of N-Myc is disordered, we utilized Alphafold for prediction and GROMACS for optimization of the N-Myc structure, thereby improving the reliability of the predicted structure. The publicly available datasets GSE49710 and GSE73517 were adopted, which contain the transcriptome data of clinical samples from 598 NB patients.

Blocking ATF4 attenuates pulmonary fibrosis by preventing lung fibroblast activation and macrophage M2 program.

Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by myofibroblasts accumulation and uncontrolled extracellular matrix (ECM) deposition. Here, we reported that activating transcription factor 4 (ATF4), a multifunctional transcription regulatory protein, is overexpressed in IPF lungs and mouse fibrotic lungs, mainly in myofibroblasts and macrophages. Haplodeletion of Atf4 in mice or blockage of Atf4 with Atf4 shRNA-loaded lentiviruses in mice reduced bleomycin (BLM)-induced pulmonary fibrosis (PF) in vivo.

Dorsomorphin (DM) inhibits the ovarian development of Portunus trituberculatus by acting on the BMP signaling pathway.

Bone morphogenic proteins (BMPs) regulate animal growth, cell proliferation and differentiation. The BMP signaling pathway plays an important regulatory role during ovarian follicle development in mammals. However, related studies in crustaceans are limited.

Human epididymis protein 4-annexin II binding promotes aberrant epithelial-fibroblast crosstalk in pulmonary fibrosis.

Invasive lung myofibroblasts are the main cause of tissue remodeling in idiopathic pulmonary fibrosis (IPF). A key mechanism contributing to this important feature is aberrant crosstalk between the abnormal/injured lung epithelium and pulmonary fibroblasts. Here, we demonstrate that lungs from patients with IPF and from mice with bleomycin (BLM)-induced pulmonary fibrosis (PF) are characterized by the induction of human epididymis protein 4 (HE4) overexpression in epithelial cells.

Characterization of a Natural Accession of with Hybridization and Agronomic Evaluation.

, valued for its perennial nature, broad adaptability, strong cold tolerance, and high economic value in forage production, plays a crucial role in combating grassland degradation, desertification, and salinization. Using morphological and cytogenetic methods, this study evaluated the cold tolerance, post-harvest regeneration capacity, and perennial characteristics of the accession 20HSC-Z9 in the Harbin region of China from 2020 to 2023. This accession exhibited a germination rate of over 90% and a 100% green-up rate, with purple coleoptiles indicating its strong cold tolerance.

Catalytic Advancements in NH Selective Catalytic Oxidation: The Impact of Cu-Induced Lattice Distortion in TiO Crystal Structure.

A series of x Cu-TiO obtained from the sol-gel method were tested for NH selective catalytic oxidation (NH-SCO). Its performance was higher than that of supported Cu/TiO-Im and solvent-free Cu/TiO-SF. The catalyst exhibits better water resistance at 300 °C.

MGST1 facilitates novel KRAS inhibitor resistance in KRAS-mutated pancreatic ductal adenocarcinoma by inhibiting ferroptosis.

Background: Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal cancer with a low 5-year survival rate. Treatment options for PDAC patients are limited. Recent studies have shown promising results with MRTX1133, a KRAS inhibitor that demonstrated potent antitumor activity in various types of tumors with KRAS mutation.

Low-condensation diesel use contributes to winter haze in cold regions of China.

The application of low-condensation diesel in cold regions with extremely low ambient temperatures (-14 to -29 °C) has enabled the operation of diesel vehicles. Still, it may contribute to heavy haze pollution in cold regions during winter. Here we examine pollutant emissions from low-condensation diesel in China.

Associations of different combinations of moderate-vigorous physical activity and muscle-strengthening activity with mortality among US lung cancer survivors.

Background: To investigate the associations of different combinations of moderate to vigorous physical activity (MVPA) and muscle strengthening activity (MSA) with all-cause and cancer mortality among lung cancer survivors.

Methods: This nationwide prospective cohort study used data from the US National Health Interview Survey 2009-2018. A total of 785 lung cancer survivors were included in the study.

Enhancing Pseudomonas cell growth for the production of medium-chain-length polyhydroxyalkanoates from Antarctic krill shell waste.

Antarctic krill shell waste (AKSW), a byproduct of Antarctic krill processing, has substantial quantity but low utilization. Utilizing microbial-based cell factories, with Pseudomonas putida as a promising candidate, offers an ecofriendly and sustainable approach to producing valuable bioproducts from renewable sources. However, the high fluoride content in AKSW impedes the cell growth of P.