Reprogramming of microspore fate via BBM-BAR1 for highly efficient in vivo haploid induction.

Haploid induction (HI) through stress-treated microspore culture has gained significant attention for over half a century, yet the molecular mechanism underlying microspore fate transition for androgenesis remains poorly understood. Here, we demonstrate that microspore-specific expression of BABY BOOM (BBM) is sufficient to induce microspore cell fate transition and in vivo androgenesis in both tobacco and rice, effectively bypassing the requirement for stress treatment. We further identify BBM-activated Androgenesis Regulator 1 (BAR1) as a novel downstream effector of BBM that promotes microspore reprogramming.

Anatomical Determinants of Postoperative In-the-Bag IOL Position: Introducing a Novel Biometric Index.

Purpose: To investigate whether a novel preoperative biometric index that integrates lens geometry and the relative position of the capsular bag can predict the postoperative in-the-bag intraocular lens (IOL) position proportion coefficient and subsequently the final IOL position.

Design: Retrospective study.

Subjects: This study included 322 eyes of 322 patients undergoing uneventful cataract surgery with in-the-bag implantation of an aspheric Tecnis IOL (Johnson & Johnson Vision, Santa Ana, CA).

Impact of Post-Procedural Atrial Arrhythmia on Long-Term Cardiac Function and Quality of Life Following Patent Foramen Ovale Closure.

Background: Patent foramen ovale (PFO) is a common remnant of the embryonic development of the heart with an underestimated potential for morbidity and mortality. This study aimed to investigate the long-term impact of atrial arrhythmia (AA), a common complication after PFO closure, on cardiac function and quality of life (QoL) through a retrospective clinical analysis.

Methods: Patients who underwent percutaneous PFO closure between January 2022 and June 2024 were retrospectively identified.

The mA-SFRP2-NFAT/TOX axis governs T cell exhaustion in gastric cancer.

Background: While immune checkpoint inhibitors (ICIs) have revolutionized cancer therapy, their efficacy in gastric cancer (GC) remains limited, underscoring the need for mechanistic biomarkers of immune evasion.

Methods: We analyzed mA RNA modification patterns in the TCGA-STAD cohort, stratifying patients into three subtypes. Functional assays (including CRISPR-based SFRP2 modulation, NFAT/TOX reporter systems, and ex vivo T-cell exhaustion models) were employed to dissect the m1A-SFRP2-NFAT/TOX axis.

Thymic stromal lymphopoietin in leukemia: A double-edged sword?

Thymic stromal lymphopoietin (TSLP) is a cytokine expressed by various cell types. It is involved in the development of allergies, autoimmune diseases, and cancers. TSLP affects immune responses and disease pathogenesis by binding to a heterodimeric receptor complex.

Challenges with the use of postbiotics/parabiotics in food industry.

The food industry is increasingly interested in postbiotics/parabiotics because of their ability to preserve and enhance functional food production. These substances contain several bioactive compounds with immune system-regulating, antimicrobial, antioxidant, prebiotic, and anticancer properties. Despite its potential benefits, incorporating postbiotics/parabiotics into food poses various obstacles that must be overcome.

Accuracy comparison of six intraocular lens formulas using total and standard keratometry measurements with the iolmaster 700.

This study aimed to evaluate the accuracy of six intraocular lens (IOL) calculation formulas using total keratometry (TK) and standard keratometry (K) measurements from the IOLMaster 700 in various ocular subgroups. A total of 212 eyes were analyzed. The mean absolute error (MAE), standard deviation (SD) of prediction error, and the median absolute error (MedAE) were calculated for each formula.

Physiological Responses and Histopathological Changes in Narrow-Clawed Crayfish () Under Acute Thermal Stress.

To investigate thermal tolerance, physiological responses, and molecular mechanisms of the narrow-clawed crayfish () under acute thermal stress, the were acutely exposed to 4 different temperature groups-15 °C (control), 20 °C (T20), 25 °C (T25), and 30 °C (T30)-across 6 time points (3 h, 6 h, 12 h, 24 h, 48 h, and 72 h). Survival rates were recorded at each interval. Subsequent analyses comprised: (1) Hemolymph biochemical parameter determination; (2) hepatopancreatic antioxidant capacity assessment; (3) hepatopancreatic histopathology; and (4) comparative transcriptomics analysis of the hepatopancreas.

Machine learning-based prediction of G-CSF-induced hematopoietic stem cell mobilization outcomes in healthy volunteers.

Haematopoietic stem cell transplantation (HSC-T) has been established as a fundamental therapeutic intervention for a wide range of hematological malignancies and disorders, with a proven record of efficacy spanning over five decades. Peripheral blood (PB) has become the haematopoietic stem cell (HSC) source of choice, surpassing bone marrow, owing to its cost-effectiveness, reduced invasiveness, higher cell yields, and shorter hospitalizations. Clinically, granulocyte-colony stimulating factor (G-CSF) administration is the standard procedure for inducing HSC mobilization.

Anterior segment characteristics and spatial variations of different crystalline lens diseases.

Introduction: To evaluate anterior chamber and crystalline lens morphology in patients with cataracts, lens subluxation (LS), and microspherophakia (MSP) using three anterior segment (AS) imaging instruments, and to develop a novel method for assessing the spatial characteristics of crystalline lens position through anterior chamber morphology measurements.

Methods: We examined 98 eyes (19 normal, 25 cataracts, 27 LS, 27 MSP) using AS optical coherence tomography (AS-OCT), ultrasound biomicroscopy (UBM), and IOLMaster700. Measurements included corneal thickness, anterior chamber parameters, and lens thickness (LT).

Psoralidin induces pyroptosis in both tumor cells and macrophages as well as enhances nature killer cell cytotoxicity to suppress hepatocellular carcinoma.

Psoralidin is a major component of the traditional Chinese medicine Psoraleae Fructus, which is derived from the dried mature fruit of the leguminous plant Psoralea corylifolia L. and possesses many pharmacological effects, including anti-tumor effects. However, the mechanism through which psoralidin protects against hepatocellular carcinoma (HCC) remains unclear.

MCOA: A Comprehensive Multimodal Dataset for Advancing Deep Learning in Corneal Opacity Assessment.

Corneal opacity remains a major global cause of vision impairment. Its severity is typically assessed subjectively by clinicians using slit lamp examinations of the anterior segment. While anterior segment optical coherence tomography (AS-OCT) provides high-resolution cross-sectional images of the cornea, capturing subtle structural changes, the combination of AS-OCT images with anterior segment photographs delivers a more comprehensive view of the cornea.

Inactivation mechanisms of carvacrol on Salmonella Typhimurium and its combined inhibitory effects with blue light-405 nm for chicken meat preservation.

Salmonella Typhimurium (S. Typhimurium) is a major contributor responsible for food contamination and foodborne illnesses. In this study, the antibacterial activity of natural compound carvacrol (CAR) against S.

AP2M1 is a prognostic marker associated with cell cycle arrest and the tumor immune microenvironment in acute myeloid leukemia.

Acute myeloid leukemia (AML) is a heterogeneous clonal disease of hematopoietic progenitor cells and the most common malignant myeloid disease in adults. Although significant progress has been made in treatment, the outlook remains bleak, and new therapeutic targets need to be sought. AP-2 complex subunit mu (AP2M1) is a core component of the clathrin-mediated endocytic machinery, AP2M1 plays a critical role in cancer progression.

Prediction of pH Gradient Elution of Ion Exchange Chromatography for Antibody Charge Variants Separation Based on Salt Gradient Elution Experiments.

Mechanistic modeling of ion exchange chromatography (IEC) is a promising technique to improve process development. However, when considering the pH influence, model prediction becomes challenging due to the multiple pH-dependent parameters and complex interactions. In order to more effectively predict the pH gradient elution behavior, a two-step model calibration strategy was proposed for the pH-dependent steric mass action (SMA) model with the empirical correlations of characteristic charge ν and equilibrium coefficient k.

Simultaneous Improvement of Mechanical Strength, Toughness, and Self-healability of Elastomers Enabled by F─H-Bond-Based Nanoconfinement.

There are often trade-offs among high mechanical strength, high toughness, and efficient self-healing. Herein, we present a biomimetic strategy utilizing F─H bonds for nanoconfinement to achieve the simultaneous enhancement of these conflicting properties. The mechanical strength, toughness, and self-healing efficiency of a fluorinated crosslinked poly(urethane-urea) (CPUU-FA) elastomer are improved 1.

Comparison of plasma sterilizer and conventional laminar flow room in allogeneic hematopoietic stem cell transplant recipients.

Patients receiving allogeneic hematopoietic stem cell transplantation (allo-HSCT) are typically placed in a laminar air flow room until hematopoietic reconstitution occurs. In this study, we compared the differences in clinical outcomes between patients receiving allo-HSCT in a conventional laminar flow room ( = 200) and those receiving allo-HSCT in a plasma sterilizer environment ( = 201). The overall infection rates (20.

Advancements in mechanisms and drug treatments for fibrodysplasia ossificans progressiva.

Fibrodysplasia ossificans progressiva (FOP) is a rare genetic disorder characterized by congenital bilateral malformation of the large toe and progressive, extensive, and irreversible heterotopic ossification (HO) of soft tissues throughout the body, leading to severe disabilities. FOP is caused primarily by mutations in activin A receptor type 1 (), also known as activin-like kinase 2 (), which encodes a receptor belonging to the bone morphogenetic protein (BMP) type I family. However, the continuous and complex process of HO in FOP is not yet fully understood, which has impeded the development of therapeutic drugs.

Clinical outcomes of plate-haptic diffractive multifocal toric IOL in cataract eyes with long axial length and corneal astigmatism.

The prospective study is aimed to determine the clinical outcomes of plate-haptic diffractive multifocal toric intraocular lens (IOL) implantation in cataract patients with long axial length and corneal astigmatism. Visual outcomes, defocus curves, contrast sensitivity, rotational stability, vector changes of refractive astigmatism and patient satisfaction were assessed over 3 months. Vector analysis was performed by the Alpins method.

METTL3-mediated m6A modification of PAK6 drives cervical cancer progression through activating MAPK14.

N6-methyladenosine (m6A) is the most prevalent epitranscriptomic modification in mammalian mRNA. Recent studies have revealed that m6A modification is involved in cervical cancer (CC) pathogenesis, yet the precise mechanisms remain largely unexplored. In this study, we identified DDX5 as a binding partner of the methyltransferase METTL3 in CC cells, demonstrating that DDX5 interacts with METTL3 to regulate global m6A levels.

FK506 activates the BMP signaling pathway to regulate ovarian development in Portunus trituberculatus.

Bone morphogenic proteins (BMPs) play important regulatory roles in the development of follicles in mammals. However, studies on the roles of BMPs in ovarian development in low-level aquatic animals, especially the swimming crab Portunus trituberculatus, are limited. In this study, a BMP Ⅰ-type receptor-specific activator (tacrolimus, FK506) was administered at different concentrations via in vivo injection, and the effects of FK506 on the regulation of the BMP signaling pathway during ovarian development in P.

Targeting SHP-1-Mediated Inhibition of STAT3 and ERK Signalling Pathways Rescues the Hyporesponsiveness of MHC-I-Deficient NK-92MI.

Natural Killer (NK) cells have shown promising prospects in 'off-the-shelf' cell therapy, particularly the NK-92 cell line, which can serve as a foundation for the next generation of universal chimeric antigen receptor (CAR)-engineered NK products. A key strategy for generating universal cellular products is the elimination of the beta-2-microglobulin (B2M) gene, which encodes a component of MHC class I molecules (MHC-I) that plays a role in the presentation of foreign antigens and in the 'licensing' or 'education' of NK cells. To functionally study the impacts of MHC-I deficiency on NK-92, we generated a B2M knockout (KO) NK-92MI (B-92) cell line and compared the multidimensional properties of B2M KO and wild-type NK-92MI cells in terms of biological phenotypes, effector functions, and transcriptomic signatures.

Association between atherogenic index of plasma and future cardiovascular disease risk in middle-aged and elderly individuals with cardiovascular-kidney-metabolic syndrome stage 0-3.

Background: Cardiovascular disease (CVD) is strongly correlated with plasma atherogenic index (AIP); however, there is limited literature exploring the association between trajectories of change in AIP and the risk of CVD. This study aimed to investigate whether changes in AIP are associated with CVD in individuals with cardiovascular-kidney-metabolic (CKM) syndrome stage 0-3.

Methods: Data were sourced from the China Health and Retirement Longitudinal Study (CHARLS), aimed to compile high-quality microdata on individuals and households aged 45 and older in China.

Utilization of Crab Shell Waste for Value-Added Bioplastics by -Based Microbial Cell Factories.

With the development of the aquatic products processing industry, 6-8 million tons of shrimp and crab shell waste are produced globally annually, but, due to the lack of high-value conversion technology, crab shells are often discarded in large quantities as a by-product of processing. -based microbial cell factories are capable of biosynthesis of high-value products using a wide range of substrates; however, there is currently no reliable fermentation model for producing high-value chemicals using crab shell waste by strains. In this study, we first explored the culture conditions of shell fermentation using KT2440 through single-factor and orthogonal experiments, and the optimized fermentation parameters obtained are given as follows: a temperature of 30 °C, fermentation time of 42 h, substrate solid-liquid ratio of 7%, and rotational speed of 200 rpm.