Decoding Xylem Development in Flowering Plants: Insights From Single-Cell Transcriptomics.

Single-cell RNA sequencing (scRNA-seq) has emerged as a transformative tool for decoding plant development, particularly in elucidating xylem differentiation. By capturing transcriptomic changes at single-cell resolution, scRNA-seq enables reconstruction of developmental trajectories across diverse plant tissues. In this review, we summarize recent advances in the application of scRNA-seq to study both primary and secondary xylem development in monocots and eudicots.

Triptorelin associated adverse events evaluated using FAERS pharmacovigilance data.

Triptorelin, a gonadotropin-releasing hormone(GnRH) agonist, is approved by the US Food and Drug Administration(FDA) for treating advanced prostate cancer, endometriosis, and central precocious puberty(CPP) in children aged ≥ 2 years. This study aimed to characterize the real-world adverse event(AE) profile associated with triptorelin using data from the FDA Adverse Event Reporting System(FAERS). We conducted a retrospective pharmacovigilance study utilizing FAERS reports from the first quarter of 2004 to the third quarter of 2024 (2004Q1-2024Q3).

Straw humification coefficient and its relation to soil carbon and nitrogen status.

Straw return is one of the crucial practices available for enhancing soil organic carbon and nitrogen, biological activity, and nutrient availability. While the impact of straw return on soil organic carbon has been extensively studied, it remains unclear how soil organic carbon (C) and nitrogen (N) status contributes to straw decomposition in different climatic regions. We conducted a meta-analysis of 464 comparison data sets from 126 peer-reviewed publications to explore the decomposition characteristics of straw in different climatic regions of China.

Gut Microbiota-Mediated Bile Acid Metabolism Regulates Colorectal Cancer Liver Metastasis by Altering Neutrophil Recruitment.

The gut microbiome is frequently dysregulated in colorectal cancer (CRC). Gut microbiota can modify bile acids (BAs), which is a group of metabolites that have been linked to tumorigenesis and progression. Here, we observed a significant deficiency in gut bacteria with bile salt hydrolase (BSH) activity in patients with liver metastatic CRC (CRLM).

Infrared image super resolution with structure prior from uncooled infrared readout circuit.

Infrared images, rich in temperature information, have a broad range of applications. However, limitations in infrared imaging mechanisms and the manufacturing processes typically prevent uncooled infrared detector arrays from exceeding a resolution of one megapixel. Consequently, designing an efficient infrared image Super-Resolution (SR) algorithm is of significant importance.

Thermal protection of the plastid-encoded RNA polymerase complex by PAP9 ensures chloroplast function in Arabidopsis.

The Plastid-encoded RNA polymerase (PEP) complex is a multi-subunit machinery essential for chloroplast biogenesis and photosynthetic gene transcription. Its structural integrity and enzymatic activity rely critically on a suite of PEP-associated proteins (PAPs). Mutations in most PAP-encoding genes typically lead to severe albinism and developmental arrest in Arabidopsis thaliana.

Wettability-Switchable Azobenzene Inverse Opal Film via Interfacial Engineering.

Precise control of surface wettability and droplet dynamics is essential for the development of intelligent interfaces and fluidic devices. However, most existing systems suffer from limited wettability modulation, an irreversible response, or complex fabrication. Herein, we report an azobenzene inverse opal film capable of reversible wettability and adhesion switching under alternating UV/vis irradiation.

Cognitive function, sleep characteristics and their relationship in older adults with insomnia and anxiety.

To analyze cognitive function, sleep characteristics and the relationship in older adults with insomnia and anxiety. A total of 99 participants were included in this study, including 50 patients with simple insomnia and 49 patients with insomnia combined with anxiety. General demographic information was collected and the degree of cognitive function was assessed using the Montreal Cognitive Assessment Scale (Moca) and the Pittsburgh Sleep Quality Scale (PSQI).

Temperature-dependent flexible hybrid capacitors based on Ni(OH)@NiS electrode materials.

The morphology of the electrode materials seriously affect their structure and performances. The application of two-dimensional Ni(OH) nanomaterials in supercapacitors is hindered by their low conductivity and limited active sites. Herein, we synthesized Ni(OH)@NiS composites a two-step hydrothermal route.

Beyond the Pleura: A Case Report of Type 1 Brugada Phenocopy in the Setting of Empyema.

We report a case of a 50-year-old male with a history of Hodgkin lymphoma in the neck region (in remission), ischaemic heart disease, and hypothyroidism who presented with right-sided pleuritic chest pain, cough, fever and night sweats. Imaging revealed a large right-sided loculated pleural effusion. Electrocardiogram (ECG) demonstrated a spontaneous Type 1 Brugada pattern, absent in prior recordings.

The effect of rotenone contamination on high-resolution mitochondrial respiration experiments.

Background: High-resolution respirometry is commonly used in skeletal muscle research and exercise science to measure mitochondrial respiratory function in both permeabilized muscle fibers and isolated mitochondria. Due to the low throughput and high cost of the most used respirometer, the Oroboros 2k (O2k), multiple experiments are often conducted within the same chamber in short succession. Despite this, no methodological consideration has been given for the potential contamination of inhibitors, used to investigate the contribution of specific complexes within the electron transport chain, between experiments.

Factors associated with sedentary behavior among breast cancer patients during chemotherapy: A cross-sectional study based on COM-B model.

Sedentary behavior (SB) is one of the prevalent unhealthy behaviors among breast cancer (BC) patients during chemotherapy and is associated with a variety of adverse health outcomes. Employing a comprehensive theoretical framework to identify the key determinants of SB is a critical step in developing targeted, evidence-based interventions. The purpose of this study was to explore the determinants of SB by using the Capability, Opportunity, Motivation - Behavior model.

CD31 regulates metabolic switch in Treg migration attenuates rheumatoid arthritis.

CD31 (PECAM-1) plays a critical role in T cell migration, whilst its immunoreceptor tyrosine inhibitory motifs (ITIMs), Y663 and Y686, are recognised for their roles in endothelial function, the precise mechanism in regulating immune cell remains elusive. Here, we demonstrate that CD31 is essential for Treg migration. Upon ITIM engagement, CD31 activates and interacts with the protein tyrosine phosphatase SHP2.

Increasing the Limits of Energy and Safety in Polynitro Tetrazoles by Incorporating Fluorine Atom.

Fluorodinitromethyl is a crucial substituent for energetic materials (EMs), known for its superior thermal stability compared to other polynitro groups. In this study, we incorporated the fluorodinitromethyl group into a tetrazole backbone to achieve a balance between stability and energetic performance. The compound 2-(dinitromethyl)-5-(fluorodinitromethyl)-2H-tetrazole and its corresponding energetic salts were designed, synthesized through optimized routes, and thoroughly characterized.

mutation leads to different genders of twinning: a case report and literature review.

Background: Loss-of-function variants in protein phosphatase 1 regulatory subunit 12A () can lead to urogenital and/or brain malformation syndrome (UBMS). When UBMS individuals exhibit genital abnormalities, it is combined with disorders of sex development (DSD). To report a variation in a case of 46,XY twins exhibiting different phenotypes of genital development.

Polarization beam splitter based on a double-groove slanted grating under normal incidence.

In this paper, we propose a one-dimensional polarization beam splitting grating under normal incidence with excellent polarization characteristics and a high diffraction efficiency. The main structure is a double-groove slanted grating. We employed a rigorous coupled-wave analysis and simulated annealing algorithm for numerical calculations of the grating.

Analytical equations for the three diffraction orders of a fused silica grating at the second Bragg incidence.

The physical analytical equation is always a pursuit of designing a grating for applications. We presented analytical equations to describe the diffraction process of a deep-etched fused silica grating under the second Bragg incidence by using the simplified modal method, particularly in a special case where the effective refractive index of mode 1 might be equal to mode 2. We found that the joint effect of modes 1 and 2 has no effect on the diffraction efficiency for the 0th order and the -2nd order.

Exploring the accuracy of whole-body MRI in assessing treatment response in metastatic breast cancer: A narrative review.

Objectives: Breast cancer is the second most common cancer worldwide, with metastasis leading to significant morbidity, mortality and healthcare costs. Bone metastasis remains particularly prevalent, estimated to appear in 70 % of cases. Therefore, accurate monitoring of treatment response in metastatic breast cancer is critical.

CHI3L3 immature neutrophils inhibit anti-tumor immunity and impede immune checkpoint blockade therapy in bone metastases.

Bone metastases remain incurable and respond poorly to immune checkpoint blockade (ICB) therapy. The impact of neutrophil maturation in cancer, particularly in refractory tumors including bone metastases, is not well understood. Here, we observed the predominance of immature neutrophils in the bone metastasis microenvironment of both mouse models and cancer patients.

Interplay between C-reactive protein responses and antibiotic prescribing in people with suspected infection.

Background: Serial measurements of C-reactive protein (CRP) are often taken in hospitals to assess recovery from infection, but their utility remains debated. Previous studies, including our development of CRP centile reference charts for suspected bloodstream infections (BSI), suggest variability in CRP responses across infection types. Here we investigated the association between serial CRP percentile changes, antibiotic prescribing patterns, and patient outcomes in a large cohort with suspected infection, acknowledging that CRP is one of multiple factors in clinical decision-making.

Baseline erythropoietin level predicts Luspatercept response in Chinese patients with myelodysplastic syndrome: a propensity score-matched analysis.

Background: Luspatercept is a novel erythroid maturation agent approved for treating transfusion-dependent anemia in myelodysplastic syndrome (MDS). However, predictive biomarkers and real-world data in Asian populations remain limited.

Methods: We conducted a retrospective cohort study of 66 MDS patients from two Chinese centers, including 22 patients treated with luspatercept and 44 matched controls.

High level of carbapenem resistance and transmission ability of in multidrug-resistant isolates from China.

The emergence of metallo-beta-lactamase gene variants, such as , has posed a significant challenge to bacterial infection treatment, drawing considerable attention in public health. This study aims to investigate the resistance mechanism of -carrying clinical in China. -harboring carbapenem-resistant (CRE) isolates were collected from a multicenter study across China.

A Novel Nontargeted Screening Strategy for New Psychoactive Substances: A Case Study of Synthetic Cannabinoids Based on Electron-Activated Dissociation High-Resolution Mass Spectrometry and Intelligent Elucidation.

Nontargeted screening of new psychoactive substances (NPSs) has always been a challenging task, typically involving data acquisition, the extraction of suspicious peaks, and mass spectrometry elucidation in the screening process. The ongoing advancement of instrument acquisition technology and data analysis methods has resulted in an increasing amount of sample data requiring manual elucidation, significantly reducing the efficiency of forensic identification work and leading to issues such as missed detections and false positives. This study proposed a novel nontargeted screening strategy that is capable of automatically elucidating the NPS classes and chemical structures of unknown designer drugs.

Electrospun Parallel, Crossed Fibers for Promoting Cell Adhesion and Migration.

Electrospun fibers, possessing biomimetic characteristics similar to fibrous extracellular matrices, have attracted widespread attention as scaffold materials for skin tissue engineering. The topographical structure of electrospun fibers plays a critical role in determining cell behavior. However, the effects of fiber topography on human skin fibroblasts (HSFs) remain unclear.

Tonsillar hypertrophy-associated false-positive syphilis serology in children: a clinical and immunologic analysis of 5 cases.

Introduction: Serologic testing for Treponema pallidum is the basis of diagnosis for syphilis. False-positive serologic results, however, may result in inaccurate diagnosis, with a substantial impact on clinical management and patient status.

Methods: This study reports 5 cases of children who presented with tonsillar hypertrophy and had false-positive results on the syphilis chemiluminescence immunoassay (CLIA).