A Safe and Broad-spectrum SARS-CoV-2 mRNA Vaccine with a New Delivery System for In-situ Expression.

Since the outbreak of SARS-CoV-2 in late 2019, the cumulative number of confirmed cases worldwide has surpassed 778 million, and the number of deaths has exceeded 7 million, posing a significant threat to human life and health while inflicting enormous losses on the global economy. At the stage where sequential immunization is recommended, there is a pressing demand for mRNA vaccines that can be rapidly adapted to new sequences, are easy to industrialize, and exhibit high safety and effectiveness. We developed a lipid nanoparticle (LNP) system, designated as WNP, which facilitates essentially in situ expression at the injection site and results in lower levels of pro-inflammatory factors in the liver, thus enhancing its safety compared to liver-targeted alternatives.

An injectable nanosuspension based on orthoester and biomimetic carboxymethyl chitosan nanoparticles for chemo/thermo-synergistic tumor therapy.

The primary objective of this study was to develop a nanosuspension based on orthoester compounds (OE) and carboxymethyl chitosan (CMCS) for the combined treatment of tumors. Initially, OE was synthesized as a liquid pharmaceutical excipient. Subsequently, nanoparticles were formulated using CMCS and loaded with mitoxantrone (MIT).

Nanomedicine Targeting the Warburg Effect: Advanced Strategies for Cancer Therapy.

Cancer remains the foremost cause of mortality globally, characterized by un-controlled cellular proliferation driven by oncogenic mutations and other factors. These mutations disrupt cellular homeostasis, leading to a spectrum of adverse physiological responses. A key feature of cellular metabolism in cancer is the Warburg effect, in which cancer cells preferentially rely on glycolysis for ATP production, even in the presence of oxygen, to meet their elevated metabolic demands.

DHYZ modulates hippocampal cholinergic pathway acetylation to ameliorate cognitive deficits post-ischemic stroke in rats.

Ischemic stroke is a serious cerebrovascular disease that is often accompanied by debilitating sensorimotor deficits and persistent cognitive deficits, which seriously affect patients' quality of life. DHYZ, a traditional Chinese herbal formula, has shown significant efficacy in restoring neurological function in ischemic regions of the brain, but its potential for improving poststroke cognitive impairment remains underdeveloped. In this study, the middle cerebral artery occlusion/reperfusion (MCAO/R) model was used to reproduce the pathological process of ischemic stroke in humans.

[Effect analysis of innovative model on perioperative pain management in prostate cancer patients with hematuria undergoing prostatic artery embolization].

Objective: To investigate the effect of innovative perioperative pain management on prostate cancer patients with hematuria undergoing prostatic artery embolization (PAE).

Methods: A total of 60 patients undergoing PAE in the Interventional Therapy Department of General Hospital of Eastern Theater Command from May 2024 to January 2025 were selected by convenience sampling method and randomly divided into the intervention group and the control group, with 30 patients in each group. The control group received traditional pain management of nursing.

Lactylation modification in lung cancer: A review of current research and future directions (Review).

Lung cancer is a common malignancy that poses risks to human health and quality of life. The primary treatment options currently available include surgery, chemotherapy and radiotherapy. However, the aggressive metastatic nature of the disease combined with the development of drug and radiation resistance results in suboptimal survival outcomes.

MAIT cell enrichment in Lynch syndrome is associated with immune surveillance and colorectal cancer risk.

Tissue microenvironment characteristics associated with elevated risk of colorectal cancer (CRC) in Lynch syndrome (LS) are poorly characterized. We applied the multimodal single cell sequencing platform ExCITE-seq to define the colonic cellular composition and transcriptome of LS carriers with and without a history of CRC compared with general population controls. Our analysis revealed widespread remodeling in LS that included striking expansion of epithelial stem and progenitor cells, and loss of fibroblast populations.

MAIT cell enrichment in Lynch syndrome is associated with immune surveillance and colorectal cancer risk.

Tissue microenvironment characteristics associated with elevated risk of colorectal cancer (CRC) in Lynch syndrome (LS) are poorly characterized. We applied the multimodal single cell sequencing platform ExCITE-seq to define the colonic cellular composition and transcriptome of LS carriers with and without a history of CRC compared with general population controls. Our analysis revealed widespread remodeling in LS that included striking expansion of epithelial stem and progenitor cells, and loss of fibroblast populations.

DyFiLM: A framework to handle the distribution shifts on dynamic graphs with hypernetworks.

Dynamic graph representation learning has garnered increasing attention since dynamic graphs can accurately reflect the changes and evolutionary processes in the real world. Existing approaches typically train a fixed model to capture temporal patterns and then utilize the fixed model to infer future evolution. They assume that the dynamic graph evolves with the same law over time.

Iron Coordination in PtFeCoNiMo High-Entropy Alloys Modulates Platinum Local Environments for pH-Universal Hydrogen Evolution Reaction.

Developing cost-effective, stable hydrogen evolution reaction (HER) electrocatalysts effective across pH-Universal remains challenging. This work reports a one-pot synthesized Pt-Fe-Ni-Mo-Co high-entropy alloy catalyst supported on Ketjen Black (HEA@KB) featuring stacked nanoparticles. By systematically tuning the iron coordination, the optimized HEA@KB demonstrates outstanding HER activity with low overpotentials of 12.

ApoE4 Upregulates GSK-3β to Aggravate Alzheimer-Like Pathologies and Cognitive Impairment in Type 2 Diabetic Mice.

Background: The apolipoprotein E (ApoE) ε4 allele and type 2 diabetes mellitus (T2DM) are independent risk factors for Alzheimer's disease (AD), the most prevalent neurodegenerative disorder in the elderly. The T2DM patients carrying the ApoE ε4 allele exhibit heightened activation of platelet glycogen synthase kinase-3β (GSK-3β), a key downstream kinase in the insulin signaling pathway, along with more severe cognitive deficits. This observation suggests an intrinsic link between ApoE ε4, GSK-3β, and cognitive dysfunction.

A nomogram for predicting the risk of chemoradiotherapy-associated thrombocytopenia in patients with esophageal cancer: a real-world cohort study.

Background: Thrombocytopenia is a common hematological toxicity in esophageal cancer (EC) patients receiving radiation therapy.

Objectives: The purpose of this study was to construct and validate a nomogram for predicting the incidence of thrombocytopenia in EC patients receiving radiotherapy.

Design: A retrospective study.

Polydopamine-coupled NT3-derived oriented conductive scaffolds with immunomodulatory properties accelerate peripheral nerve regeneration.

Peripheral nerve injury is a complex condition presenting significant clinical treatment challenges due to the limited regenerative capacity of peripheral nerves. Nerve conduits have been seen as a promising strategy to overcome the shortage of other treatment options (e.g.

Targeting ARPC1B Cancer Stem Cells to Sensitise Pancreatic Cancer to Gemcitabine Treatment.

ARPC1B cancer stem cells (CSCs) in pancreatic cancer are identified as a subpopulation resistant to gemcitabine. In our study, drug repositioning, molecular docking, and surface plasmon resonance (SPR) technique jointly revealed that CK-636 can directly target ARPC1B protein with high affinity. In vitro cytotoxicity, ex vivo organoid cultures, in vivo xenograft and orthotopic gemcitabine-resistant pancreatic cancer model demonstrated that combination therapy of gemcitabine plus CK-636 showed a superior anti-tumor effect compared with gemcitabine monotherapy.

Hierarchical biomass-derived carbon framework with asymmetric ZnCo dual-atom pairs for synergistic polysulfide adsorption and catalysis in Lithium-sulfur batteries.

Lithium‑sulfur (LiS) batteries have recently attracted ever-increasing attention owing to their ultrahigh specific energy and substantial cost benefits of sulfur. However, the sluggish redox kinetics from the critical conversion of soluble long-chain lithium polysulfide to solid-state LiS remains a fundamental challenge. Herein, hierarchical porous nitrogen-doped carbon frameworks with asymmetric ZnCo dual-atom pairs (Zn-Co/NC) were successfully constructed using biomass-derived porous carbon as structural matrix through in situ growth and assisted pyrolysis strategy.

Fatty acid 2-hydroxylase facilitates rotavirus uncoating and endosomal escape.

Despite the clinical significance of many nonenveloped viruses, the molecular mechanisms of their internalization and membrane penetration are not well understood. Rotaviruses (RVs) are nonenveloped double-stranded RNA viruses and the leading cause of severe dehydrating diarrhea in infants and young children. We identified fatty acid 2-hydroxylase (encoded by ) in the fatty acid 2-hydroxylation pathway as a proviral gene that supports RV infection.

Dynamic nomogram predicts sepsis risk in patients with acute liver failure: Analysis of intensive care database with external validation.

Background: Acute liver failure (ALF) with sepsis is associated with rapid disease progression and high mortality. Therefore, early detection of high-risk sepsis subgroups in patients with ALF is crucial.

Aim: To develop and validate an accurate nomogram model for predicting the risk of sepsis in patients with ALF.

A Citrate Synthase Splice Variant Rewires the TCA Cycle to Promote Colorectal Cancer Progression.

Metabolic reprogramming, notably alterations in the tricarboxylic acid (TCA) cycle, has emerged as a hallmark of cancer that supports tumor growth and metastasis. Despite the TCA cycle being a classical central metabolic pathway, further exploration is needed to fully elucidate the intricate manifestations and contributory mechanisms of TCA cycle rewiring in colorectal carcinogenesis. Herein, we identified a splicing isoform of citrate synthase (CS), CS-ΔEx4, and unveiled its role in TCA cycle dysregulation in colorectal cancer (CRC).

Complete genome sequences of strains Y and N, capable of conjugated diene biohydrogenation.

We report the complete genome sequences of two acetogenic strains, Y and N, isolated from river sediment. Under anaerobic acetogenic conditions, strain Y biohydrogenates isoprene, while strain N transforms 1,3-butadiene to 1-butene.

Intrinsically Temperature-Insensitive and Highly Sensitive Flexible Wireless Strain Sensor.

Accurate strain monitoring in environments with coexisting mechanical deformation and temperature fluctuations─such as solid rocket propellants, battery enclosures, and human ligaments─remains a longstanding challenge for flexible electronics. Conventional strain sensors suffer from significant thermal drift due to the intrinsic temperature dependence of their sensing materials, limiting their reliability in wireless and implantable applications. Here, we report an intrinsically temperature-insensitive, highly sensitive, wireless flexible strain sensor based on near-field communication technology.

Neoadjuvant chemoradiotherapy with capecitabine and irinotecan guided by UGT1A1 status in patients with locally advanced rectal cancer: 5-year update of the CinClare trial.

Background: The optimal regimen and chemotherapy intensity are still under investigation for neoadjuvant treatment of locally advanced rectal cancer (LARC). The CinClare trial has demonstrated improved pathologic complete response (pCR) with the addition of irinotecan to neoadjuvant chemoradiotherapy (CRT) guided by uridine diphosphate glucuronosyltransferase 1A1 (UGT1A1) genotype in LARC. Here, we report the 5-year follow-up outcomes of the CinClare study.

Revealing the Relationship between Interfacial Morphology Degradation and Unsatisfied Stability in Phenethylamine-Treated Perovskite Solar Cell.

The surface passivation with phenethylammonium halides has proven effective in reducing defect density and trap-assisted recombination, enabling outstanding efficiency in perovskite solar cells. Despite its promise, it is ambiguous whether this strategy can improve the stability of devices under rigorous conditions, such as light soaking and thermal aging. A critical issue is whether the interface is robust in structure and morphology under light illumination.

A copper-based single-atom material effectively controls plant diseases with nearly zero soil residue and low phytotoxicity.

A growing population necessitates the development of sustainable agriculture, which requires achieving atom economy in pesticide delivery, fertilization, and so on. To this end, we focus on single-atom materials (SAMs) to enhance atom utilization within agricultural systems. In this study, we report a novel pesticide for plants, a single-atom copper (Cu) formulation, by employing a precipitation-equilibrium-driven (K-driven) method to anchor Cu onto a calcium carbonate (CaCO) carrier.

Injectable biomimetic nano-hydrogel composite based on copper selenide nanoparticles and carboxymethyl chitosan for synergistic chemo-photothermal cancer therapy.

This study reports the synthesis and characterization of an injectable nano-hydrogel composite (m@NPs-HG) based on selenium nanoparticles (Se NPs) and carboxymethyl chitosan (CMCS) nanoparticles for enhanced cancer therapy. Selenium nanoparticles were stabilized using CMCS to form copper selenide nanoparticles (CSe NPs), while doxorubicin (DOX)-loaded CMCS nanoparticles (CD NPs) were encapsulated within cancer cell membranes to generate biomimetic nanoparticles (m@NPs). Subsequently, CSe NPs and m@NPs were integrated into a hydrogel via crosslinking with CuCl, resulting in the formation of m@NPs-HG.

Diagnostic utility of PSMA immunohistochemistry in colorectal mass biopsy.

Rendering a diagnosis of invasion can be challenging in a small biopsy of a large colorectal mass-forming lesion. However, this diagnosis can have major implications in the management of patients with rectal cancer. Prostate-specific membrane antigen (PSMA) is associated with tumor neoangiogenesis.