Developing a prognostic model of glutamine metabolism-related genes associated with clinical features and immune status in melanoma.

Introduction: Melanoma exhibited a poor prognosis due to its aggression and heterogeneity. The effect of glutamate metabolism promoting tumor progression on cutaneous melanoma remains unknown. Herein, glutamine metabolism-related genes (GRGs) were identified followed by constructing a prognostic model for melanoma via bioinformatics analysis.

The role of gut microbiota dysbiosis in drug-induced brain injury: mechanisms and therapeutic implications.

Drug-induced brain injury (DIBI) results from toxicity, interactions or misuse and is increasingly linked to gut-microbiota dysbiosis operating via the gut-brain axis. Disturbed microbial balance drives three core mechanisms-oxidative stress, neuroinflammation and metabolic dysfunction-leading to blood-brain barrier leakage, neuronal loss and cognitive impairment; antibiotics, antineoplastics and psychoactive drugs further promote bacterial translocation and systemic inflammation. Microbial metabolites and neurotransmitters also mediate post-injury anxiety and depression.

The Role of the Wiskott-Aldrich Syndrome Protein Family in Cancer Development, Invasion, and Metastasis.

Emerging evidence indicates that cancer metastasis is governed by distinct genetic mechanisms that operate independently of those regulating carcinogenesis and cancer proliferation. The Wiskott-Aldrich syndrome protein (WASP) family members significantly contribute to the regulation of actin cytoskeleton (AC) dynamics and cancer cell invasion and metastasis. Recent research has demonstrated that WASP and its homolog, the WASP family verprolin-homologous protein (WAVE), function as a master regulator and a key scaffolding protein, bringing together the various components of metastatic signaling complexes, thereby facilitating the development of solid tumors.

3D Printing via Melt Extrusion Deposition Facilitates the Use of Extended-Release Profiles in Preclinical Research and Development.

Traditional methods for developing modified-release (MR) formulations involve numerous iterations and large quantities of drug substances, which pose considerable challenges in exploration settings. Given the growing necessity for modified-release (MR) formulations in the pharmaceutical industry, particularly during the preclinical research and development phase, modified-release strategies may serve as attractive alternatives to discontinuing clinical development and could mitigate the costs and time associated with identifying new drug candidates. This study specifically explores the application of melt extrusion deposition (MED) 3D printing technology as a rapid prototyping platform for creating extended-release (ER) oral dosage forms tailored for the preclinical phase.

Antigenic and glycan-binding characteristics of norovirus GII.20 capsid protein VP1.

Human norovirus (NoV) is one of the major pathogens causing acute gastroenteritis. The GII.20 NoV VP1 reveals a close evolutionary relationship with that of the widely prevalent GII.

An Increasing Prevalence of Non-GII.4 Genotypes Causing Norovirus Gastroenteritis Outbreaks - Beijing Municipality, China, 2017-2024.

Objectives: Since 2014, non-GII.4 norovirus genotypes have increasingly challenged the predominance of GII.4, particularly in Asia.

Research on target detection for autonomous driving based on ECS-spiking neural networks.

In response to the increasing demands for improved model performance and reduced energy consumption in object detection tasks relevant to autonomous driving, this research presents an advanced YOLO model, designated as ECSLIF-YOLO, which is based on the Leaky Integrate-and-Fire with Extracellular Space (ECS-LIF) framework. The primary aim of this model is to tackle the issues associated with the high energy consumption of traditional artificial neural networks (ANNs) and the suboptimal performance of existing spiking neural networks (SNNs). Empirical findings demonstrate that ECSLIF-YOLO achieves a peak mean Average Precision (mAP) of 0.

Identification of a recombinant GII.13[P21] norovirus strain: molecular dynamic simulations indicate that gene mutations shifted its spectrum of binding to host receptor glycans.

Human norovirus is a pervasive pathogen that causes global outbreaks of viral gastroenteritis. Previous studies have suggested that histo-blood group antigens (HBGAs) can interact with human norovirus, facilitating its entry of host cells and significantly affecting its evolution. In this study, the complete genome sequence of recombinant GII.

Advancements in rheumatoid arthritis immunotherapy: a comprehensive bibliometric analysis of research trends and hotspots (2003-2023).

Objectives: To analyse research trends and developments in rheumatoid arthritis (RA) immunotherapy through a comprehensive bibliometric analysis of literature from 2003 to 2023.

Methods: Publications related to RA immunotherapy were retrieved from Web of Science Core Collection database using specified search terms. Bibliometric analysis was performed with VOSviewer, CiteSpace, Pajek, and R packages to examine publication patterns, international collaborations, research hotspots, and emerging trends.

Association between mitophagy and inflammasome in uric acid nephropathy.

Objective: This study was recruited to investigate the role of mitophagy in activating NLRP3 inflammasome in the kidney of uric acid (UA) nephropathy (UAN) rats.

Methods: This study developed a uric acid nephropathy (UAN) rat model divided into five groups: Negative control (NC), UAN model (M), UAN + autophagy inhibitor (3-MA), UAN + lysosome inhibitor (CQ), and ROS scavenger (N-acetylcysteine, N). H&E staining assessed renal structure, ROS levels were measured with 2, 7dichlorofluorescin diacetate, and ELISA measured serum markers (, , cystatin , , , ).

The accuracy of DNA methylation detection in endometrial cancer screening: A systematic review and meta-analysis.

Objective: DNA methylation is the hallmark of early endometrial cancer and can be detected through non-invasive methods. The present study systematically reviewed the efficacy of DNA methylation detection for endometrial cancer screening through exfoliative cytology.

Methods: A systematic literature review was performed through the following databases from inception to October 7, 2024: PubMed, Embase, and the Cochrane Library.

Enhanced visible-light-driven photocatalytic degradation via in-situ AIS/ZIS high-low junctions.

The I-III-VI QDs Ag-In-S (AIS) exhibits excellent properties in photocatalysis because of the adjustable band gap, wide light absorption range, and multiple active sites. Introducing homologous or heterogeneous ions not only derives the composition into quaternary/ quinary quantum dots but also generates new sulfide QDs to form composites, which is an effective strategy to promote photoactivity. In this work, we in-situ synthesized the AIS/ZIS (AgInS/ZnInS) composite photocatalyst by introducing Zn and changing the reaction temperature.

Comprehensive Screening and Validation of Stable Internal Reference Genes for Accurate qRT-PCR Analysis in under Diverse Biological Conditions and Environmental Stresses.

is among the world's most destructive pests. For accurate qPCR and gene expression studies, the selection of stable and appropriate reference genes is crucial. However, a thorough evaluation of potential reference genes for use in research is lacking.

Experimental models for preclinical research in kidney disease.

Acute kidney injury (AKI) and chronic kidney disease (CKD) are significant public health issues associated with a long-term increase in mortality risk, resulting from various etiologies including renal ischemia, sepsis, drug toxicity, and diabetes mellitus. Numerous preclinical models have been developed to deepen our understanding of the pathophysiological mechanisms and therapeutic approaches for kidney diseases. Among these, rodent models have proven to be powerful tools in the discovery of novel therapeutics, while the development of kidney organoids has emerged as a promising advancement in the field.

Agrochemical control of gene expression using evolved split RNA polymerase. II.

Agrochemical inducible gene expression system provides cost-effective and orthogonal control of energy and information flow in bacterial cells. However, the previous version of Mandipropamid inducible gene expression system (Mandi-T7) became constitutively active at room temperature. We moved the split site of the eRNAP from position LYS179 to position ILE109.

SNORD3A Regulates STING Transcription to Promote Ferroptosis in Acute Kidney Injury.

Acute kidney injury (AKI) signifies a sudden and prolonged decline in kidney function characterized by tubular cell death and interstitial inflammation. Small nucleolar RNAs (snoRNAs) play pivotal roles in oxidative stress and inflammation, and may play an important role in the AKI process, which remains elusive. an elevated expression of Snord3a is revealed in renal tubules in response to AKI and demonstrates that Snord3a deficiency alleviates renal injury in AKI mouse models.

Molecular, behavioral, and growth responses of juvenile yellow catfish (Tachysurus fulvidraco) exposed to carbamazepine.

Carbamazepine (CBZ) is an anticonvulsant medication used to treat epilepsy and bipolar disorder. Due to its persistence and low removal rate in wastewater treatment plants, it is frequently detected in the environment, raising concerns regarding its potential adverse effects on aquatic organisms and ecosystems. In this study, we aimed to assess the impact of CBZ on the behavior and growth of juvenile yellow catfish Tachysurus fulvidraco, a native and economically important species in China.

Tracking moisture contents in the pollution layer on a composite insulator surface using hyperspectral imaging technology.

Electrical insulators used in transmission lines and outdoor substations are exposed to severe environmental pollution, which significantly increases the risk of power system failure, especially when the pollution layer is highly humid due to adverse weather conditions. The focus of this paper is to establish an effective method for assessing the moisture content (MC) in pollution layers as it serves as a crucial indicator for evaluating the risk of failure in insulators. Hyperspectral imaging (HSI) technology with a spectral range of 371.

A novel cancer-germline gene DAZL promotes progression and cisplatin resistance of non-small cell lung cancer by upregulating JAK2 and MCM8.

Germline-specific genes are usually activated in cancer cells and drive cancer progression; such genes are called cancer-germline or cancer-testis genes. The RNA-binding protein DAZL is predominantly expressed in germ cells and plays a role in gametogenesis as a translational activator or repressor. However, its expression and role in non-small cell lung cancer (NSCLC) are unknown.

Alamandine attenuates ovariectomy-induced osteoporosis by promoting osteogenic differentiation via AMPK/eNOS axis.

Background: Alamandine is a newly characterized peptide of renin angiotensin system. Our study aims to investigate the osteo-preservative effects of alamandine, explore underlying mechanism and bring a potential preventive strategy for postmenopausal osteoporosis in the future.

Methods: An ovariectomy (OVX)-induced rat osteoporosis model was established for in vivo experiments.

Genotyping and traceability analysis of norovirus in Yantai between 2017 and 2019.

To investigate the diversity and evolution of noroviruses in Yantai in recent years, this study focused on the coat protein regions of norovirus-positive samples with nucleic acid detection (cycle threshold) values below 30 between 2017 and 2019. A total of 81 sequences were obtained for genotyping. Initially, a high-throughput sequencing approach was established to perform the whole-genome sequencing of multiple typical diarrheal strains.