Ligand-mediated structural modulation and membrane stabilization of LPA1 receptor: Insights from molecular dynamics simulations.

Lysophosphatidic acid (LPA) is an important bioactive signaling molecule that activates six distinct G protein-coupled receptors (GPCRs), among which the LPA1 subtype possesses high therapeutic target potential due to its critical roles in malignant tumors, pulmonary fibrosis, inflammation, and neuropathic pain. Recent studies have shown that the small molecule ONO-0740556 can effectively disrupt the overall structure of LPA1 in solution; however, its effects in a native membrane environment remain unclear. According to the Traditional Chinese Medicine Systems Pharmacology Database (TCMS), Dan Shen(Salvia miltiorrhiza) exhibits anti-inflammatory and anti-pulmonary fibrosis properties, and its natural compound cryptoxanthin may serve as a novel LPA1 inhibitor.

Stereo-cell: Spatial enhanced-resolution single-cell sequencing with high-density DNA nanoball-patterned arrays.

Single-cell sequencing technologies have advanced our understanding of cellular heterogeneity and biological complexity. However, existing methods face limitations in throughput, capture uniformity, cell size flexibility, and technical extensibility. We present Stereo-cell, a spatial enhanced-resolution single-cell sequencing platform based on high-density DNA nanoball (DNB)-patterned arrays, which enables scalable and unbiased cell capture at a wide input range and supports high-fidelity transcriptome profiling.

Hepatic CBP/p300 Orchestrate Amino Acid-Driven Gluconeogenesis through Histone Crotonylation.

The role of amino acid metabolism dysregulation in the development of type 2 diabetes remains elusive. Here, significant associations of human CREBBP/EP300 gene polymorphisms with circulating amino acid and glucose levels are reported. Through integrated transcriptomic, metabolomic, and CUT&Tag analyses, the molecular mechanisms underlying these correlations are investigated.

ZmPOD5 positively regulates drought tolerance by modulating ROS production.

Drought stress is an important abiotic stress affecting maize (Zea mays L.) growth and productivity. Class III peroxidases (PODs) are plant-specific enzymes that play crucial roles in plant growth, development, and responses to abiotic stress.

Increased Propofol Sensitivity Associated With Hearing Loss in Patients Undergoing Vestibular Schwannoma Surgery: A Retrospective Study.

Background: Propofol is widely used in neurosurgery, with its dosage typically based on patient weight and variability. While factors like age, sex, and cognitive function are known to influence propofol requirements, the impact of preoperative hearing function remains underexplored. This study investigates the relationship between hearing loss and propofol sensitivity in vestibular schwannoma surgery patients.

Comparison of the effectiveness of immersive and non-immersive virtual reality in the treatment of vertigo in patients with peripheral vestibular dysfunction: a systematic review and meta-analysis.

Background: Vertigo is the most common clinical manifestation in patients with peripheral vestibular dysfunction (PVD), and severe episodes may be accompanied by nystagmus, tinnitus, and hearing loss, which can seriously affect quality of life. Virtual reality (VR) technologies (immersive or non-immersive) play an important role in improving vertigo in patients with PVD, but the comparative effectiveness of VR technologies with different levels of immersion is unknown.

Objective: To investigate the effectiveness of VR technology at different immersion levels in reducing vertigo symptoms in patients with PVD.

Structures of the measles virus polymerase complex with non-nucleoside inhibitors and mechanism of inhibition.

The measles virus (MeV), a highly contagious non-segmented negative-sense RNA virus in the Paramyxoviridae family, causes millions of infections annually, with no approved antivirals available. The viral polymerase complex, comprising the large (L) protein and the tetrameric phosphoprotein (P), is a key antiviral target. We determined the cryo-electron microscopy structures of the MeV polymerase complex alone and bound to two non-nucleoside inhibitors, ERDRP-0519 and AS-136A.

Application effect of short-term traffic flow prediction method based on CNNBLSTM algorithm.

Reduced forecast efficiency and accuracy are the result of traditional traffic flow prediction algorithms' inability to adequately capture the spatiotemporal characteristics and dynamic changes of traffic flow. To address this problem, this study proposes a short-term traffic flow prediction method based on an improved convolutional neural network and a bidirectional long short-term memory algorithm. The method firstly identifies, repairs and decomposes the abnormal traffic flow data by smoothing the estimation threshold and adaptive noise integration empirical modal decomposition method to improve the data quality and stability.

Titanium-catalyzed radical-type deuteriodeboronation of arylboronates.

Deuterated aromatic compounds have extensive applications in organic synthesis and the pharmaceutical industry. Nevertheless, the development of cost-effective transition metal catalysis remains pressing. In this study, we present an unprecedented titanium-catalyzed deuteriodeboronation of arylboronates, which enables the efficient synthesis of deuterated aromatic rings with excellent functional group compatibility and high yields.

Pre-ligand assembly domain-derived recombinant protein of TNFR2 inhibits the expansion of CD4+ Foxp3+ regulatory T cells.

There is compelling evidence that tumor necrosis factor (TNF) receptor type II (TNFR2) mediates the stimulatory effect of TNF on the activation of CD4+ Foxp3+ regulatory T cells (Tregs). Tregs with high TNFR2 expression are critical components of tumor microenvironment, where they promote tumor progression by impeding antitumor immune responses. Thus, selectively targeting TNFR2 has emerged as a promising strategy to inhibit Treg activity and enhance antitumor immune responses.

Attentional dysfunction arises from right frontocentral and occipital network connectivity in Parkinson's disease.

Background: The Flanker task measures visuospatial attention and assesses the attentional network by distinguishing pathways for enhancing information at attended regions and suppressing information at unattended ones (Kopp et al., 1996). In Parkinson's disease (PD), the attentional network is impaired due to dysfunctional fronto-subcortical circuits connected to the basal ganglia, disrupting response selection and inhibition.

Targeted suppression of glioma by ultralow-dose x-ray-induced photodynamic therapy and gold-based nanoclusters in preclinical models.

The diffuse and infiltrative nature of high-grade gliomas poses ongoing challenges in treatment and management. Radiotherapy is an important glioma treatment, with a standard radiotherapy dose of 60 gray. However, high-dose radiotherapy is associated with radiation-induced side effects on normal tissue.

Development and evaluation of machine learning models for individualized prediction of myopia control efficacy treated with overnight orthokeratology.

Purpose: The primary objective of this study is to develop a predictive model utilizing fundamental clinical and ocular measurements to predict the effect of overnight orthokeratology on myopia control. Accordingly, this study aims to assist ophthalmologists in selecting adolescent myopia control methods.

Methods: This retrospective study used one-year follow-up data of 225 myopia children treated with orthokeratology.

miR-125b-5p sensitizes colorectal cancer to anti-PD-L1 therapy by decreasing TNFR2 expression on tumor cells.

The microRNA miR-125b-5p, recognized as a tumor suppressor, has demonstrated the ability to curb the proliferation of various types of cancer cells. Our latest research has revealed that miR-125b-5p also impedes the proliferation and functionality of CD4 + Foxp3+ regulatory T cells by reducing the expression of tumor necrosis factor receptor 2 (TNFR2) on regulatory T cells. To explore the potential of miR-125b-5p to suppress tumor growth by targeting TNFR2 on cancer cells, we overexpressed the levels of miR-125b-5p in mouse colorectal cancer cells.

Identification of hub biomarkers in coronary artery disease patients using machine learning and bioinformatic analyses.

Understanding the molecular underpinnings of CAD is essential for developing effective therapeutic strategies. This study aims to identify and analyze differentially expressed hub biomarkers in the peripheral blood of CAD patients. Based on RNA-seq datasets from the Gene Expression Omnibus database, machine learning algorithms including LASSO, RF, and SVM-RFE were applied.

Human umbilical cord mesenchymal stem cell therapy for atopic dermatitis through inhibition of neutrophil chemotaxis.

Background: Atopic dermatitis (AD) management is significantly challenging due to the high prevalence, chronicity, and recurrent nature of the disease, and limited options for its treatment. Human umbilical cord mesenchymal stem cells (hUC-MSCs) exhibit potential effects against AD; however, the mechanisms underlying these effects remain largely unexplored.

Methods: AD mouse models were established using 1-chloro-2,4-dinitrobenzene (DNCB) and ovalbumin (OVA).

Repeated-dose toxicity and immunogenicity evaluation of a recombinant subunit COVID-19 vaccine (ZF2001) in rats.

Coronavirus disease 19 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), had given rise to a massive epidemic. Owing to the high morbidity and mortality of COVID-19 and the lack of effective therapies, safe and effective vaccination is the optimum choice for controlling this epidemic and preventing infection. The protein subunit vaccine ZF2001, which targets the receptor-binding domain (RBD) protein of SARS-CoV-2, has a significant protective effect against COVID-19.

How does the farmland management rights mortgage loan affect food security: Based on the evidence of major grain producing areas in China.

The people are the foundation of the nation, and grain is vital for their survival. Food security is crucial for national stability and well-being. This study utilizes panel data from 1,234 counties in China's primary grain-producing regions, covering the years 2010 to 2021, to conduct a theoretical analysis and empirical examination of the impact of farmland management rights mortgage loans (FMRML) on food security, employing a difference-in-differences (DID) model.

Polyaspartic Acid-Calcium-Lanthanum Complexes Induce Antibacterial Remineralization of Dentin and In-Depth Occlusion of Dentinal Tubules.

Dental caries is a global oral dilemma leading to demineralization of tooth hard tissues and exposure of dentinal tubules (DTs). Multifunctional restorative strategies, including remineralization, antibacterial properties, and DT occlusion, are urgently needed for dental caries management but remain a significant challenge. Herein, the polyaspartic acid-calcium-lanthanum suspension (5 g L-3.

Characterizing G-type antiferromagnetism quantitatively with optical second harmonic generation.

Antiferromagnetism has become a promising candidate for the next generation electronic devices due to its thermal stability, low energy consumption, and fast switching speed. However, the canceling of the net magnetic moment in antiferromagnetic order presents great challenge on quantitative characterization and modulation, hindering its investigation and application. In this work, utilizing the optical second harmonic generation (SHG) in a wide temperature range, the integrated differential phase contrast scanning transmission electron microscopy, and first-principles calculations, we performed a quantitative study on the evolution of non-collinear antiferromagnetic order in BiFeO films with a series of strains.

[Mechanisms of tumor immune microenvironment remodeling in current cancer therapies and the research progress].

The cellular and molecular components of the tumor immune microenvironment (TIME) and their information exchange processes significantly influence the trends of anti-tumor immunity. In recent years, numerous studies have begun to evaluate TIME in the context of previous cancer treatment strategies. This review will systematically summarize the compositional characteristics of TIME and, based on this foundation, explore the impact of current cancer therapies on the remodeling of TIME, aiming to provide new insights for the development of innovative immune combination therapies that can convert TIME into an anti-tumor profile.

Quantitative H NMR optimization for high-throughput metabolite analysis in industrial bioprocess monitoring.

Quantitative H NMR (H qNMR) is an ideal tool for bioprocess monitoring because it can comprehensively detect and quantify diverse metabolites that significantly influence bioprocess performance. However, the long experiment time associated with the H qNMR, due to the long longitudinal relaxation time (T1) of some metabolites, does not meet the requirements for high-throughput analysis. We developed a high-throughput H qNMR method for bioprocess analysis using a short relaxation delay (D1) to reduce analytical time and a correction factor (k) to compensate for incomplete relaxation.

Prognostic implications of alternative splicing events and key splicing factors in head and neck squamous cell carcinoma.

The incidence of head and neck squamous cell carcinoma (HNSCC) remains high, accompanied by low 5-year survival rates. Identifying prognostic factors is essential for advancing personalized treatment approaches. Increasing evidence implicates aberrant alternative splicing (AS) plays a key role in tumor progression.