Pleurotus citrinopileatus polysaccharides ameliorate DSS-induced ulcerative colitis by improving intestinal barrier and inflammation modulation via NF-κB pathway.

The aim was to investigate the pharmacological effects of a polysaccharide isolated from Pleurotus citrinopileatus on ulcerative colitis (UC). One polysaccharide, CP-2-2, was isolated and purified from P. citrinopileatus body fruit using chromatographic methods.

Single-Cell RNA Sequencing Reveals Granzyme K CD8 T Cells as a Target for Mitigating Plaque Instability Following Radiation.

Background: Cardiac issues following radiotherapy are increasingly prevalent among patients with thoracic cancer and coronary disease. However, the mechanisms underlying radiotherapy-induced plaque instability and changes in plaque characteristics on imaging remain unclear. This study used single-cell RNA sequencing to identify key features of vulnerable plaques following radiotherapy.

Blood pressure in the first 6 hours for older adults with stroke after endovascular therapy: a pooled analysis of the DEVT and RESCUE BT randomized clinical trials.

Optimal systolic blood pressure (SBP) targets after endovascular therapy (EVT) for stroke in older adults (≥ 65 years) remain undefined. This study assessed age-stratified associations between early post-EVT SBP (first 6 h) and outcomes. Post hoc analysis of two trials.

SLB-msSIM: A Spectral Library-Based Multiplex Segmented SIM Platform for Single-Cell Proteomic Analysis.

Mass spectrometry (MS)-based single-cell proteomics, while highly challenging, offers unique potential for a wide range of applications to interrogate cellular heterogeneity, trajectories, and phenotypes at a functional level. We report here the development of the spectral library-based multiplex segmented selected ion monitoring (SLB-msSIM) method, a conceptually unique approach with significantly enhanced sensitivity and robustness for single-cell analysis. The single-cell MS data is acquired by a multiplex segmented selected ion monitoring (msSIM) technique, which sequentially applies multiple isolation cycles with the quadrupole using a wide isolation window in each cycle to accumulate and store precursor ions in the C-trap for a single scan in the Orbitrap.

Revealing the heterogeneity of electrode surfaces using photoluminescence electrochemical microscopy.

Photoluminescence-based electrochemical microscopy enables micrometer-scale probing of electrode heterogeneity. Our results revealed a correlation between the potential-negative differential photoluminescence profile and the corresponding cyclic voltammogram. We further elucidated the structure-activity relationship linking interfacial heterogeneity to the electrocatalytic performance of electrodeposited CuO as a proof-of-concept experiment.

Genome-wide association study reveals candidate genes associated with egg-laying performance in Wuhua yellow chicken.

Egg production traits are economically critical in poultry farming. However, the genetic mechanisms underlying these traits in indigenous chicken breeds remain largely unknown. In this study, we conducted a genome-wide association study (GWAS) using whole-genome sequencing data from 315 Wuhua yellow chickens, an indigenous breed characterized by low egg production but considerable genetic diversity.

Effect Evaluation and Mechanism Study of Polyether Surfactant-Assisted Supercritical Carbon Dioxide-Oil Miscibility Based on the Visible Vanish of Interface Measurement Method.

Research on reducing the minimum miscible pressure (MMP) of CO and oil is imperative for the advancement of CO flooding, which can effectively enhance the recovery of low-permeability reservoirs and enable CO geological storage. However, the conventional methods of determining the MMP (e.g.

Near-Infrared-Absorbing B-N Lewis Pair-Functionalized Anthracene Derivatives: Electronic Structure and Second-Order NLO Properties.

Creating optical materials with linear and nonlinear properties has always been a focus of attention in the scientific community. Recently, Pf (x) displays unusual photophysical properties, such as near-infrared absorption and emission, and transparency in the visible light region, etc. However, from the perspective of improving material performance, its structure-property correlation remains to be fully investigated.

Correction: Optineurin overexpression ameliorates neurodegeneration through regulating neuroinflammation and mitochondrial quality in a murine model of amyotrophic lateral sclerosis.

[This corrects the article DOI: 10.3389/fnagi.2025.

Accuracy of maxillary repositioning in le fort I osteotomy using a mixed reality based navigation system versus CAD/CAM splints and patient-specific surgical plates: A preclinical study.

Objective: Accurate maxillary repositioning is essential for successful outcomes in orthognathic surgery. Current studies on mixed reality (MR) navigation are limited and lack comparison. This study developed an MR-based navigation system and compared it with computer-aided design/computer-aided manufacturing (CAD/CAM) splints and patient-specific surgical plates (PSSPs) in Le Fort I osteotomy.

M2c Macrophages Mediate YAP1 to Promote Vascularized Bone Regeneration in Distraction Osteogenesis.

Distraction osteogenesis (DO) is an efficient approach to addressing craniofacial deformities and bone defects. M2c macrophages play a crucial role in the formation of bone tissue; however, the underlying mechanism during the immunoregulation related to angiogenic osteogenesis remains unclear. In this study, we first isolate the cell culture medium from M2c macrophages to induce HUVECs and confirm the angiogenic ability.

Unlocking Insights from Meal Descriptions: Enhancing Dietary App Features with AI and Rule-Based Methods.

This study presents a novel method for labeling food items in unstructured meal descriptions using rule-based and AI-driven approaches. Results from analyses of data from a field experiment show that expert support improves meal balance. ChatGPT offers potential for food labeling, which can be used to inform app design.

Revealing the Ion Channel Switch of the Cell Membrane via Electrochemiluminescence Pattern Transformation.

Ion channels as transmembrane proteins provide an important transport pathway for specific ions through cell membranes, which are crucial to studies involving the pathophysiology of the nervous system. However, the switch between different conformational states (i.e.

Association between serum homocysteine levels and advanced hepatic fibrosis in alcohol-related liver disease: A cross-sectional study of NHANES.

Homocysteine (Hcy) can induce liver cell damage, but its relationship with alcohol-related liver disease (ALD) has rarely been reported. This study aimed to investigate the association between serum Hcy levels and advanced hepatic fibrosis in patients with ALD. We included 10,033 participants from the 1999 to 2006 National Health and Nutrition Examination Survey.

Pore connectivity evaluation and seepage characteristic of ultra deep carbonate reservoirs of permian Qixia formation in NW Sichuan basin.

Ultra-deep carbonate reservoirs in the Permian Qixia Formation of the Sichuan Basin, particularly those exceeding 7000 m in depth, have emerged as a significant focus for exploration and development. However, production capacities between adjacent wells can vary by up to two orders of magnitude due to the multi-scale heterogeneity of pore connectivity, which poses challenges for accurately predicting well performance. This study utilizes thin-section analysis, high-pressure mercury injection (HPMI), scanning electron microscopy (SEM), and other techniques to investigate pore connectivity in ultra-deep reservoirs.

Development and validation of predictive models for disease-free survival and overall survival in non-metastatic right-sided colon adenocarcinoma patients based on inflammatory and nutritional indices.

Background: This study developed and validated prognostic nomograms incorporating inflammatory and nutritional biomarkers to predict disease-free survival (DFS) and overall survival (OS) in patients with non-metastatic right-sided colon adenocarcinoma (NRCA).

Methods: We retrospectively analyzed NRCA patients who underwent curative resection and allocated 70% ( = 406) to training cohort and 30% ( = 172) to internal validation cohort. An external cohort from a secondary institution ( = 103) provided independent validation.

A male-essential miRNA is key for avian sex chromosome dosage compensation.

Birds have a sex chromosome system in which females are heterogametic (ZW) and males are homogametic (ZZ). The differentiation of avian sex chromosomes from ancestral autosomes entails the loss of most genes from the W chromosome during evolution. However, the extent to which mechanisms evolved that counterbalance this substantial reduction in female gene dosage remains unclear.

Self-supervised Upsampling for Reconstructions with Generalized Enhancement in Photoacoustic Computed Tomography.

Photoacoustic computed tomography (PACT) is an emerging hybrid imaging modality with potential applications in biomedicine. A major roadblock to the widespread adoption of PACT is the limited number of detectors, which gives rise to spatial aliasing and manifests as streak artifacts in the reconstructed image. A brute-force solution to the problem is to increase the number of detectors, which, however, is often undesirable due to escalated costs.

Atomically Determining the Interstitial Lithium in the Three-Dimensional Lattice of a Lithium-Ion-Conducting Solid Electrolyte.

Identifying all the possible Li sites in a crystal structure is the first step toward understanding the Li-ion migration in solid electrolytes for Li batteries. Here, we discover that the perovskite structure, a widely studied prototypic structure for solid electrolytes, may host Li ions in three different sites instead of only two sites as commonly believed. Thanks to integrated differential phase contrast (iDPC) scanning transmission electron microscopy (STEM), the Li ions that are too light to be detected in the conventional high-angle annular dark-field (HAADF) STEM are successfully visualized in the lattice.

Electrochemiluminescence Discloses Active Intermediates in Oxygen Evolution Reactions of Metal-Organic Framework.

In situ disclosure about the complex four-electron transfer of anodic oxygen evolution reaction (OER) is crucial to further optimization of electrocatalysts. However, tracing the formation and evolution of unstable intermediates remains challenging. Here, electrochemiluminescence (ECL) analysis and imaging technique are developed to in situ monitor the important intermediate hydrogen peroxide intermediate (OOH*) on transition-metal-based 7,7,8,8-tetracyanoquinodimethane (TM─TCNQ) metal-organic framework materials as OER catalysts.

Development of a reference material for simultaneous quantitative determination of multiple veterinary drug residues.

Excessive use of multiple veterinary drugs can lead to the accumulation of residues in food derived from animals, thereby posing potential risks to human health and the environment. Accurate quantification of residues from multiple veterinary drugs is essential for ensuring effective monitoring and regulatory compliance. In this study, a robust high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method was developed for the simultaneous determination of six veterinary drug residues, including florfenicol (FF), thiamphenicol (TAP), difloxacin (DIF), sulfadimidine (SDM), trimethoprim (TMP), and fenvalerate (FEN).

Accelerating Li desolvation via symmetry-broken engineering of iron single-atom catalysts for high-performance lithium-sulfur batteries.

High-energy-density lithium-sulfur (Li-S) batteries are hindered by the detrimental shuttle effect and sluggish redox conversion kinetics of lithium polysulfides (LiPSs) arising from the high energy barrier of Li diffusion kinetics at the electrode/electrolyte interface. Herein, the asymmetrically N,S co-coordinated atomic Fe sites (FeSAC-NS) are synthesized to present a strong interaction with Li based on the typical symmetry-broken electron redistribution. Comprehensive electrochemical and theoretical results reveal that FeSAC-NS as an efficient Li power pump could kinetically accelerate the dissociation of Li solvation structure and enhance the Li diffusion kinetics via the N-Fe-S active structure, further improving the bidirectional sulfur species redox electrochemistry.

D-STAR: Diffusion-based Sparse Tomographic Angular Recovery for Isotropic-Resolution Photoacoustic Imaging.

Anisotropy in imaging systems often results in directional degradation, impairing image quality and complicating subsequent analyses. While multiangle imaging has proven effective in mitigating these effects, it introduces challenges such as extended imaging times and increased excitation doses. To address these limitations in Photoacoustic Tomography (PAT), we propose a novel approach-Diffusion-based Sparse Tomographic Angular Recovery (D-STAR).

Borderline Personality Disorder Diagnoses in Facial Plastic Surgery: A Large Database Analysis.

Objective: To determine the prevalence of borderline personality disorder among patients who undergo facial plastic surgery and identify associated demographics, clinical characteristics, and outcomes.

Study Design: Retrospective cohort.

Setting: More than 80 health care organizations across the United States.