Assessment for seawall modifications to mitigate land crabs roadkills from field experiments and agent-based modeling.

The seawalls and roads between the habitats and intertidal zone impede the migration of land crabs to their spawning sites and increase the risk of roadkill in Gaomei Wetland. A pilot project aimed at mitigating roadkill risk involved modifying the landside of the seawalls from vertical to sloped. The effectiveness of the seawall modification needs to be further assessed.

Key elements and theoretical logic of application of PIC/S standards in improving drug GMP inspections in China: an analysis based on grounded theory.

Objective: Currently, China regulatory authorities are committed to improving drug inspection by learning from international advanced experiences, such as the Pharmaceutical Inspection Co-operation Scheme (PIC/S), to boost the development of the pharmaceutical industry. In the past, there has been a lack of systematic discussion and comprehensive theoretical construction of the key elements affecting the improvement of China's Good Manufacturing Practice (GMP) inspection. Through this study, the key elements and theoretical logic for applying PIC/S standards to improve China's pharmaceutical GMP inspection work are constructed.

A Hybrid Ensemble End-to-End Neural Network for Accurate Protein-Protein Interactions Prediction.

Protein-protein interactions (PPIs) are fundamental to understanding cellular mechanisms, signaling networks, disease pathways, and drug development. Over the years, numerous computational models with artificial intelligence (AI) have been developed to predict PPIs. However, these models mostly face significant challenges, such as fragmented feature extraction pipelines, inability to capture complex global relationships among proteins, and reliance on handcrafted features.

Can the accepting foreign clinical data policy improve innovation investment of pharmaceutical firms? Empirical evidence from China.

Introduction: In October 2017, China initiated adjustments to the registration procedures for imported drugs to promote accessibility to overseas drugs. In support of this decision, the National Medical Products Administration (NMPA) issued a technical guideline regarding accepting foreign clinical trial data in July 2018. Collectively referred to as the accepting foreign clinical data policy, these measures have accelerated the influx of overseas drugs into China.

CD44-targeted therapy using mP6/Rg3 micelles inhibits oral cancer stem cell proliferation and migration.

Background: This study investigates how mP6/Rg3 micelles modulate ABCB1 expression to induce ferroptosis in oral cancer stem cells (CSC) and enhance oral cancer outcomes.

Methods: Micelles targeting CD44 peptide P6 were prepared and characterized using TEM and immunofluorescence. Biocompatibility was evaluated through LIVE/DEAD staining and CCK-8 assays.

A decade of review in global regulation and research of artificial intelligence medical devices (2015-2025).

Artificial intelligence (AI) and medical devices are increasingly integrated, reshaping global diagnostic paradigms. However, the adaptive learning and opaque nature of AI technologies pose significant challenges to traditional regulatory frameworks. In response, regulatory bodies worldwide, including the U.

Real-World Evidence on Outcomes and Safety of Ropeginterferon Alfa-2b in Patients With Myeloproliferative Neoplasms: A Retrospective Cohort Study.

Background: Ropeginterferon alfa-2b-njft (ropegIFN) has demonstrated superior efficacy over hydroxyurea in polycythemia vera (PV); however, real-world data on its application across Philadelphia chromosome-negative (Ph-) myeloproliferative neoplasms (MPNs) remain limited.

Patients And Methods: This retrospective cohort study included 64 patients with Ph- MPNs (15 PV, 16 essential thrombocythemia [ET], 5 prefibrotic myelofibrosis [preMF], and 28 with overt myelofibrosis [MF]) treated with ropegIFN between October 2018 and June 2024.

Results: After a median follow-up of 5.

Self-supervised Upsampling for Reconstructions with Generalized Enhancement in Photoacoustic Computed Tomography.

Photoacoustic computed tomography (PACT) is an emerging hybrid imaging modality with potential applications in biomedicine. A major roadblock to the widespread adoption of PACT is the limited number of detectors, which gives rise to spatial aliasing and manifests as streak artifacts in the reconstructed image. A brute-force solution to the problem is to increase the number of detectors, which, however, is often undesirable due to escalated costs.

Creation of Crystalline Orientation of Tin(II) Oxide Polycrystals with High Photocatalytic Activity.

Tin(II) oxide is a promising material for photocatalytic wastewater treatment. However, the established relationships between particle size, shape, and photocatalytic activity of SnO are contradictory, indicating the influence of other factors. In this work, the effect of the SnO crystallographic texture on its band gap and photocatalytic activity was shown for the first time.

Regulatory and Influencing Factors of Digestive Function in Elderly People: Roles of the Gut Microbiota and Nutritional Interventions.

Aging is a natural and gradual biological process through which living organisms undergo physical, physiological, and sometimes psychological changes over time. Aging is commonly associated with a decline in gastrointestinal function, leading to various digestive disorders that impact the quality of life of older adults. The gut microbiota is a highly complex ecosystem that plays crucial roles in digestion, metabolic processes, immune functions, and overall health.

Maternal intestinal L. vaginalis facilitates embryo implantation and survival through enhancing uterine receptivity in sows.

Background: The embryo implantation quality during early pregnancy is the predominant factor for embryo survival and litter performance in sows. Gut microbiota is demonstrated to show a correlation to pregnancy outcomes by participating in regulating maternal metabolism. However, the specific functional microbiota and its mechanical effects on regulating embryo implantation and survival remain unclear.

D-STAR: Diffusion-based Sparse Tomographic Angular Recovery for Isotropic-Resolution Photoacoustic Imaging.

Anisotropy in imaging systems often results in directional degradation, impairing image quality and complicating subsequent analyses. While multiangle imaging has proven effective in mitigating these effects, it introduces challenges such as extended imaging times and increased excitation doses. To address these limitations in Photoacoustic Tomography (PAT), we propose a novel approach-Diffusion-based Sparse Tomographic Angular Recovery (D-STAR).

Estimation the Maximum Tolerance Activity of Blood by a Simple Algorithm Method in Pediatric Differentiated Thyroid Cancer Patients Treated With Empirical Radioactive Iodine Dosing Based on Risk Stratification.

After total thyroidectomy for pediatric differentiated thyroid cancer (DTC), the subsequent radioactive iodine (RAI) treatment is a robust therapy, the dosing of which is a major concern. This study was designed to evaluate the "as high as safe administration" (AHASA) principle of RAI treatment in pediatric DTC patients based on the maximum tolerance activity (MTA) of blood to certify dosimetry via a simple algorithm method. Twenty pediatric DTC patients were enrolled and received RAI treatment empirical dosing based on risk stratification after total thyroidectomy.

Digital light processing-printed personalized gelatin Methacryloyl/silk fibroin Methacryloyl hydrogel microneedles loaded with platelet-rich proteins for accelerated wound healing.

The hydrogel microneedles (HMNs) with good biocompatibility enables the efficient and painless delivery of bioactive substances to deeper tissue, promoting effective wound healing. However, due to the inherent variability in size, shape, and depth of wounds during practical application, for HMNs, customization is essential for optimal alignment with individual wounds to expedite the healing process. Here, we developed a personalized gelatin methacrylate/methacrylated silk fibroin (GelMA/SFMA) HMNs loaded with platelet-rich proteins (GSPMNs) to accelerate wound healing.

Advancing oral cancer care: nanomaterial-driven diagnostic and therapeutic innovations.

The advent of nanotechnology has significantly advanced the diagnosis and treatment of oral cancer, offering more precise and efficient therapeutic strategies. This review presents a comprehensive overview of recent developments in the application of nanotechnology to oral cancer management. It begins with an overview of the epidemiology of oral cancer and outlines current diagnostic and therapeutic methods.

One-Step Enrichment and Quantitative Analysis of In Vivo Protein Complexes via Dimethylpiperidine Cross-Linker DPST.

In vivo cross-linking mass spectrometry (XL-MS) enables the proteome-wide characterization of protein complexes in living cells. However, most XL-MS methods face significant sample loss during enrichment, limiting their applications to limited-quantity samples, and suffer from poor reproducibility (20%-40%), hindering precise quantification. To overcome these challenges, we developed a novel membrane-permeable cross-linker, 2,6-dimethylpiperidine disuccinimidyl tridecanoate (DPST), in which the dimethylpiperidinyl group enables one-step enrichment of cross-linked peptides via tandem mass tags (TMTs) antibody approach, eliminating sample loss from multi-step processes and allowing analysis from as few as 1E4 cells.

PVA/HA@BT hydrogel with antioxidant and antibacterial abilities for accelerating diabetic wound healing.

Diabetic wound healing is severely hindered by prolonged oxidative stress and susceptibility to bacterial infections. To address this challenge, a dual-network hydrogel (PVA/HA@BT) was developed by integrating baicalein (BCE), a natural antioxidant, and tannic acid-stabilized silver nanoparticles (TA-AgNPs) into a polyvinyl alcohol/hyaluronic acid (PVA/HA) matrix. The hydrogel enables sustained BCE release to neutralize reactive oxygen species and alleviate oxidative damage, while synergistically enhancing antibacterial efficacy through the combined action of BCE and TA-AgNPs.

Digital inclusion finance, government subsidies and R&D investment-empirical evidence based on listed Chinese pharmaceutical firms.

Digital inclusive finance, with 'universality' as its core feature, can stimulate enterprises to increase capital investment in research and development (R&D) through diversified channels. Using the digital financial inclusion index of 337 prefecture-level cities in China and the empirical data of Shanghai and Shenzhen A-share-listed pharmaceutical manufacturing enterprises from 2011 to 2022, the article empirically examines the impact mechanism of digital financial inclusion on R&D investment of pharmaceutical manufacturing enterprises from the perspective of government subsidy by using a high-dimensional fixed-effects model, a two-stage systematic GMM, and a moderated-effects test method. It is found that digital financial inclusion has a positive incentive effect on pharmaceutical manufacturing enterprises to increase R&D investment, and this positive effect still holds after the endogeneity test and robustness test.

Development and validation of an ensemble learning risk model for sepsis after abdominal surgery.

Introduction: Although their importance has gained attention, the clinical applications of methods for screening patients at high risk of sepsis after abdominal surgery have been restricted. Therefore, we aimed to develop and validate models for screening patients at high risk of sepsis after abdominal surgery based on machine learning with routine variables.

Material And Methods: The whole dataset was composed of three representative academic hospitals in China and the Medical Information Mart for Intensive Care IV (MIMIC-IV) database.

TCP1 promotes the progression of malignant tumours by stabilizing c-Myc through the AKT/GSK-3β and ERK signalling pathways.

The chaperonin tailless complex polypeptide 1 (TCP1) is a key subunit of chaperonin containing TCP1 (CCT) that regulates the folding and stability of proteins during cancer progression. Here, the prognostic significance of TCP1 was explored mainly in patients with hepatocellular carcinoma (HCC) and pancreatic ductal adenocarcinoma (PDAC). We showed that TCP1 expression was significantly greater in clinically malignant tumour tissues than in normal tissues and that high TCP1 expression was associated with poor prognosis.

Joint association of estimated glucose disposal rate and systemic inflammation response index with mortality in cardiovascular-kidney-metabolic syndrome stage 0-3: a nationwide prospective cohort study.

Background: The Cardiovascular-Kidney-Metabolic (CKM) syndrome underscores the complex interactions among metabolic disorders, kidney disease, and cardiovascular conditions. Insulin resistance (IR) and inflammation are crucial in CKM syndrome development, but their combined effect in stages 0-3 remains unclear.

Methods: Using data from the National Health and Nutrition Examination Survey (NHANES), we included 18,295 participants with CKM syndrome stages 0-3 from 10 cycles between 1999 and 2018.

Hemophagocytic Lymphohistiocytosis Secondary to Melioidosis in Paediatric Patients: A Case Series.

Background: Melioidosis caused by manifests as a variety of infectious symptoms. However, hemophagocytic lymphohistiocytosis (HLH) secondary to bacteraemic melioidosis is rarely documented in the literature. We report a series of fatal cases of melioidosis combined with HLH in one Chinese family.

Blood Oxygenation Quantification in Multispectral Photoacoustic Tomography Using a Convex Cone Approach.

Multispectral photoacoustic tomography (PAT) can create high spatial and temporal resolution images of oxygen saturation (sO2) distribution in deep tissue. However, unknown distributions of photon absorption and scattering introduces complex modulations to the photoacoustic (PA) spectra, dramatically reducing the accuracy of sO2 quantification. In this study, a rigorous light transport model was employed to unveil that the PA spectra corresponding to distinct sO2 values can be constrained within separate convex cones (CCs).

Glycoside Hydrolase Family 16 Enzyme RsEG146 From Rhizoctonia solani AG1 IA Induces Cell Death and Triggers Defence Response in Nicotiana tabacum.

Rhizoctonia solani AG1 IA is a harmful necrotrophic fungus responsible for various crop diseases, including maize and rice sheath blight, which can lead to significant production losses. However, the pathogenic mechanisms and the roles of effectors in this pathogen remain poorly understood. In this study, we identified a glycoside hydrolase 16 family gene, RsEG146, from R.