Observation of Giardia duodenalis infection in Mongolian gerbils: Cysts isolated from bovine feces.

Assemblage E of Giardia duodenalis, primarily infecting ruminants, has been relatively understudied both in vivo and in vitro. Due to unsuccessful attempts at in vitro cultivation, this study focused on establishing an economical, stable, and clinically relevant experimental animal model for Assemblage E infections. Cysts were purified from bovine feces via 33 % zinc sulfate flotation, with Assemblage E identity confirmed by gdh gene sequencing.

[Corrigendum] Protective effects of atorvastatin on cerebral vessel autoregulation in an experimental rabbit model of subarachnoid hemorrhage.

Following the publication of the above paper, an interested reader drew the authors' attention to the fact that images showing TUNEL staining of hippocampus following SAH induction in Fig. 2A, and the western blot data in Fig. 7, apparently had already been previously published in an article written by the same research group in the journal .

Dendritic Mesoporous SiO-Based SERS-Nanozyme-Mediated Immunochromatographic Detection of Foodborne Viruses with Enhanced Sensitivity across Multiple Scenarios.

Detecting low-concentration foodborne viruses in complex samples has long posed a great challenge. Here, we propose a colorimetric enhancement-surface-enhanced Raman scattering (SERS) quantitative dual-mode immunochromatographic assay (ICA), characterized by high flexibility, sensitivity, and stability, which can rapidly and accurately detect viruses in various environments, including field, home, and clinical laboratory settings. A multifunctional SERS nanozyme tag (DSAIA) is customized using dendritic mesoporous SiO as the core, which is densely loaded with AuIr catalytic particles and coated with a layer of highly active 35 nm Au nanoparticles on the exterior, thereby simultaneously achieving monodispersity, strong peroxidase activity, and a high density of efficient SERS hotspots.

Biomimetic nanoparticles coated with ScFv-modified macrophage membranes for siRNA delivery to relieve brain metastases of lung cancer.

Background: In China, lung cancer stands as the leading cause of cancer-related deaths, often resulting in brain metastases (BM) that severely compromise patients' quality of life and reduce survival outcomes. The delivery of drugs to the brain is further complicated by the blood-brain barrier (BBB). To address this, we developed EGFR single-chain fragment variable (scFv)-modified macrophage membrane liposomes (scFv-MML) encapsulating LPCAT1 siRNA (scFv-MML@LPCAT1si) as a targeted therapy for non-small cell lung cancer (NSCLC) BM.

Compensatory retinal blood flow enhancement in cognitively normal ApoE ε4 carriers.

This study aims to compare retinal vascular characteristics between ApoE ε4 carriers and non-carriers in healthy individuals. A total of 84 cognitively healthy subjects-64 non-carriers and 20 carriers of the ApoE ε4 allele-were analyzed using swept-source optical coherence tomography angiography (SS-OCTA). In the retinal vascular layer of OCTA images, parameters such as foveal avascular zone (FAZ) area, vessel perimeter, acircularity index (AI), and vascular density (FD) were quantified.

Specific genomic alterations and aggressive clinical features of sporadic thyroid carcinomas in children and adolescents: findings from an in-house cohort study.

Introduction: Papillary thyroid carcinoma is the most common pathological subtype of thyroid cancer in both children/adolescents (TCCA) and adults (TCA). TCCA manifests more aggressive and invasive behaviors than TCA, which may be attributed to specific genomic alterations.

Methods: To better understand the specific molecular, pathological and clinical manifestations of TCCA, we retrospectively analyzed a cohort of 60 patients with sporadic papillary thyroid carcinoma, including 20 TCCAs and 40 TCAs.

Long-term efficacy and immunopersistence of an -produced HPV-16/18 bivalent vaccine: an observational extension study following a randomised, double-blind Phase III clinical trial cohort.

Background: A safe and highly efficacious -produced HPV-16/18 bivalent vaccine (Cecolin®) offers a cost-effective cervical cancer prevention measure. Here, we report data on the long-term efficacy and immunopersistence up to 10 years post-vaccination.

Methods: In the Phase III clinical trial (NCT01735006), 7372 women were enrolled and randomly assigned to receive the HPV or control vaccine (hepatitis E vaccine).

Synaptotagmin III Plays a Protective Role in Neonatal Multiple Sevoflurane Exposure-Induced Cognitive Deficits in Mice.

General anesthesia in infants and young children raises concerns about potential neurodevelopmental effects, as anesthetics such as sevoflurane may impair cognitive function and increase neuroinflammation. Synaptotagmin 3 (Syt3), a protein involved in synaptic regulation, has been identified as a possible modulator of these effects. This study explores the role of Syt3 in mitigating cognitive and inflammatory outcomes following neonatal sevoflurane exposure in mice.

Integrating QTL mapping, BSA-seq and RNA-seq to identify candidate genes regulating seed storability from Dongxiang wild rice.

Rice seed storability (SS) is crucial for germplasm preservation, agricultural production, grain storage, and food security. Dongxiang wild rice ( Griff., hereafter DXWR) is a common wild rice adapted to the northernmost area worldwide and possesses strong SS.

A Sensitive Sandwich-Type Electrochemical Immunosensor for Carbohydrate Antigen 19-9 Based on Covalent Organic Frameworks.

Since carbohydrate antigen 19-9 (CA 19-9) is a significant biomarker for the clinical diagnosis and treatment of pancreatic cancer, a sensitive sandwich-type immunosensor was proposed with an epoxy functionalized covalent organic framework (EP-COF) as the antibody carrier and an electroactive COF as the signal amplification probe for the sensitive detection of CA 19-9. The flexible covalent linkage between the epoxy-functionalized EP-COF and the antibodies was employed to improve the dynamics of the antigen-antibody interaction significantly. Meanwhile, AuNPs@COF with abundant electroactive sites enhanced the current response of the immunoreaction significantly.

Breaking Down the Core Peptide of Amyloid Beta Protein with Enediyne and Mechanistic Study.

A hallmark pathological feature of Alzheimer's disease is the aberrant aggregation of amyloid-beta (Aβ) protein. While current therapeutics that target the imbalance between Aβ synthesis and clearance demonstrate the ability to clear amyloid plaques, their limited clinical efficacy underscores the need for alternative approaches that directly disrupt Aβ aggregates. In this study, we employed a hydrogel model constructed from the Aβ core peptide to validate the peptide cleavage capability of enediyne derivatives, followed by high-resolution liquid chromatography-mass spectrometry analysis of the resulting cleavage fragments.

Broad neutralizing antibody response of a monomeric spike-based SARS-CoV-2 bivalent vaccine against diverse variants.

severeacute respiratory syndrome coronavirus 2 (SARS-CoV-2) bivalent vaccines show potential against variants but lack a full understanding of the immunological mechanisms that drive broadly neutralizing antibodies (bnAbs). This study explored the immunogenicity of a bivalent vaccine in rhesus macaques, containing spike (S) proteins from the prototype (S) and chimeric S protein (S). The vaccine induced bnAbs against multiple variants, including challenging subvariants like EG.

Biocontrol of the zearalenone-producer Fusarium ipomoeae on soybean pods using an avirulent Lasiodiplodia theobromae isolate.

Background: Fusarium pod blight is emerging as a destructive disease in soybean-producing regions. Currently, strategies for the management of Fusarium pod blight are lacking. This study aimed to examine the biocontrol activity of a new avirulent Lasiodiplodia theobromae isolate for the management of Fusarium pod blight.

Machine learning unveils the impact of anthropogenic emission changes on urban PM and O: A case study in Wuhu.

PM and O are two major issues hindering air quality improvement in China. However, the response of these two pollutants to anthropogenic emission variations in the real atmosphere was not yet well understood. Here, we selected the short-term epidemic lockdown in Wuhu in 2022 as a case study and evaluated the impacts of meteorology and anthropogenic emission on PM and O using field observations combined with machine learning algorithms.

Targeting serum phosphate trajectory stratification to improve outcomes in high-risk Cardiovascular-Kidney-Metabolic-Sepsis cohorts.

Background: Sepsis patients exhibit complex clinical conditions, frequently complicated with metabolic dysregulation. Cardiovascular-Kidney-Metabolic Syndrome (C-K-M) is classified as below: stage 0, no C-K-M risk factors; stage 1, excess or dysfunctional adiposity; stage 2, metabolic risk factors (hypertriglyceridemia, hypertension, diabetes, metabolic syndrome) or moderate- to high-risk chronic kidney disease; stage 3, subclinical cardiovascular diseases (CVD) in C-K-M syndrome or risk equivalents (high predicted CVD risk or very high-risk chronic kidney diseases); and stage 4, clinical CVD in C-K-M syndrome. While high-risk patients defined by C-K-M criteria often have poor outcomes, studies seldom have classified these patients into subtypes based on metabolic profiles.

Case Report: Iptacopan in a paroxysmal nocturnal hemoglobinuria patient with severe renal insufficiency.

Paroxysmal nocturnal hemoglobinuria (PNH) is a rare disorder characterized by complement-mediated hemolysis, thrombosis, and bone marrow failure. Iptacopan, an oral factor B inhibitor, has demonstrated efficacy in managing PNH but has not been studied in patients with severe renal insufficiency. We report a case of a PNH patient with end-stage renal disease who required renal replacement therapy and had a peritoneal dialysis catheter placed during treatment.

Evaluation of the LAMP-IDE-AI platform for the detection of the HLA-B*15:02 allele in clinical application.

The HLA-B*15:02 allele is strongly associated with adverse drug reactions induced by aromatic antiseizure medications such as carbamazepine and oxcarbazepine. Consequently, it is strongly recommended to test for the presence of the HLA-B*15:02 allele before prescribing these medications. Traditional HLA genotyping methods (e.

Dysbiotic gut fungi exacerbate Klebsiella peumoniae lung infection via Dectin-1-mediated alveolar macrophage hyperactivation.

Escalating antibiotic resistance of Klebsiella pneumoniae underscores the urgent need for therapeutic strategies. Whereas gut bacterial dysbiosis exacerbates pulmonary infections, the role of gut fungi in modulating lung immunity remains understudied. Here, we demonstrate that antibiotic-induced gut fungal expansion aggravates pneumonia by enhancing alveolar macrophage-driven inflammation via Dectin-1 signaling.

Discovery of multi-target anti-gout agents from Eurycoma longifolia Jack through phenotypic screening and structural optimization.

Developing anti-gout medications that simultaneously reduce uric acid and exert anti-inflammatory effects represents a critical breakthrough for managing gout progression. Natural products with polypharmacological properties offer promising leads for drug discovery. In this study, β-carboline-1-propionic acid, a bioactive constituent of Eurycoma longifolia Jack, served as the starting point for drug design.

SWIFT-seq enables comprehensive single-cell transcriptomic profiling of circulating tumor cells in multiple myeloma and its precursors.

Multiple myeloma is a bone marrow (BM) plasma cell malignancy preceded by precursor conditions. BM biopsies are conducted infrequently and can yield inconclusive results due to technical limitations. Profiling circulating tumor cells (CTCs) may enable noninvasive routine clinical assessments but remains challenging.

Continuity of Care Needs and Dilemmatic Experiences of Lung Transplant Recipients: A Qualitative Study.

Aim: The purpose of this study was to explore and describe lung transplantation recipients' expectations and dilemmatic experiences of continuity of care.

Methods: A phenomenological research method was used to conduct face-to-face semi-structured interviews with 22 lung transplant recipients who visited the lung transplant centre of a hospital in Eastern China for follow-up visits, and NVivo 14 software was used to analyse the interview data and refine the themes.

Results: Four themes were identified in this study, including information support, maintaining collaborative relationships, standardised management, and facing multiple challenges.

Extrachromosomal DNA biogenesis is dependent on DNA looping and religation by YY1-Lig3-PARylation complex.

The link between extrachromosomal DNA (ecDNA) and tumors has been well established, and its role in cancer is of increasing interest. While ecDNA is thought to originate from genomic instability, the molecular mechanisms driving DNA end ligation during ecDNA formation and the regulatory factors controlling its selective gene packaging remain unresolved. Here, using the multi-layer perceptron model, a series of imaging strategies in human cancer cells, and clinical chip verification, we demonstrate that ecDNA biogenesis depends on transcription factor Yin Yang 1 (YY1)-mediated DNA looping coupled with religation catalyzed by DNA ligase 3 (Lig3), a mechanism that extends existing models.

SOX17 Regulates Nestin/p16 Axis to Mitigate Endothelial Senescence in Pulmonary Arterial Hypertension.

Emerging evidence indicates that endothelial cell senescence plays a critical role in the pathogenesis of pulmonary arterial hypertension (PAH). However, the underlying mechanisms and signaling pathways driving pulmonary endothelial senescence in PAH remain incompletely understood. In this study, we used a novel functional genomics approach to show that the intermediate filament protein Nestin binds to a -regulatory element (-RE) on the - locus, repressing p16 expression and mitigating cellular senescence in human pulmonary arterial endothelial cells (PAECs).

Engineered Radiative Cooling Systems for Thermal-Regulating and Energy-Saving Applications.

Highlights: This review thoroughly encapsulates the contemporary advancements in radiative cooling systems, from materials to applications. Comprehensive discussion of the fundamental concepts of radiative cooling systems, engineered materials, thermal-regulating textiles and energy-saving devices. The review critically evaluates the obstacles confronting radiative cooling systems, offering insightful and forward-looking solutions to shape the future trajectory of the discipline.