Impairment of Hyaluronan-Related Glycocalyx Inhibits Macropinocytosis in Cancer Cells via p21 Upregulation.

Macropinocytosis (MP) in cancer cells is an endocytic process for nutrient extraction initiated by membrane ruffling that supports tumor progression. Hyaluronan (HA), a major component of glycocalyx coating the surface of living cells, is reported to influence the membrane morphology. However, whether HA-related glycocalyx (HA-GCX) regulates MP through membrane reconstruction remains unclear.

Application of engineered CRISPR/Cas12a variants with altered protospacer adjacent motif specificities for the detection of isoniazid resistance mutations in .

Unlabelled: Drug-resistant tuberculosis (TB) is a major global public health concern. Although isoniazid is currently considered one of the most effective first-line drugs for TB treatment, its efficacy is limited by the emergence of resistance. Therefore, it is imperative to develop new methods for detecting drug-resistant TB.

Specific genomic alterations and aggressive clinical features of sporadic thyroid carcinomas in children and adolescents: findings from an in-house cohort study.

Introduction: Papillary thyroid carcinoma is the most common pathological subtype of thyroid cancer in both children/adolescents (TCCA) and adults (TCA). TCCA manifests more aggressive and invasive behaviors than TCA, which may be attributed to specific genomic alterations.

Methods: To better understand the specific molecular, pathological and clinical manifestations of TCCA, we retrospectively analyzed a cohort of 60 patients with sporadic papillary thyroid carcinoma, including 20 TCCAs and 40 TCAs.

Correction: The composition and predictive function of the fecal microbiota in female donkeys across different reproductive cycles.

[This corrects the article DOI: 10.3389/fmicb.2025.

Locking small solvents by hypercrosslinked polymers towards sterically hindered organogels.

Sterically hindered organogels are promising as coatings, adhesives and structural materials since they can overcome the low strength and poor stability of traditional gel materials. However, limited by the mesh size of the polymer network, it is a significant challenge to entrap small solvents to preparing sterically hindered organogels. Herein, a hypercrosslinked polyurea network with small mesh size (~1.

Increasing proportion of mildly aged population in rural mitigates farmland abandonment in the farming-pastoral ecotone of northern China.

Rural population aging has emerged as a widespread phenomenon, which can lead to farmland abandonment and pose unprecedented challenges to agricultural production and ecological sustainability in the farming-pastoral ecotone of northern China (FPENC). The dynamic changes in farmland abandonment from 2000 to 2020 were systematically explored using a trajectory-based land use change detection approach. Binary logit regression models were employed to analyze the driving mechanism of the current farmland abandonment based on 1,195 questionnaires, and then random forest models were used to predict future farmland abandonment trends under various scenarios.

Modeling Mycobacterium tuberculosis pathogenesis in lung epithelial organoids reveals strain-specific host responses and intercellular crosstalk.

Emerging studies have identified alveolar epithelial cells as a conducive niche for Mycobacterium tuberculosis (Mtb) replication and spread during early infection. However, the host-pathogen interactions and intercellular crosstalk within the lung epithelial microenvironment remain inadequately understood. Here, we developed a lung epithelial organoid coculture model and exposed it to the virulent H37Rv strain or the avirulent Bacillus Calmette-Guérin (BCG) strain to investigate tuberculosis (TB) pathogenesis.

Hard Yet Flexible Anti-Icing Coatings with Superior Wear Resistance and Anti-Corrosion Properties.

Anti-icing coatings are increasingly being used in aerospace applications, polar expeditions, and offshore wind power. However, their mechanical strength and corrosion resistance often decrease in harsh environments, limiting their long-term durability. In this study, we developed a hard yet flexible anti-icing coating that demonstrated exceptional wear resistance and anticorrosion properties.

Application of Microwave Ablation Combined With Chai Hu Qing Gan Tang in the Treatment of Idiopathic Granulomatous Mastitis.

To investigate the efficacy of microwave ablation (MVA) combined with Chai Hu Qing Gan Tang (CHQGT) for idiopathic granulomatous mastitis (IGM). 480 patients were divided into the CHQGT combination group (CHQGT + MVA), corticosteroid combination group (glucocorticoids + MVA) and control group (glucocorticoids), with 160 cases in each group. Data on patient information, treatment effects, adverse effects and breast appearance were collected.

The composition and predictive function of the fecal microbiota in female donkeys across different reproductive cycles.

The microorganisms residing in the gastrointestinal tract of monogastric herbivores play a vital role in nutrient absorption and maintaining the host's health. However, the quantitative and functional establishment of these microorganisms in female donkeys across different reproductive cycles has not yet been examined. Knowledge regarding the composition and function of gut microbiota in female donkeys during different reproductive cycles remains limited.

A novel molecularly imprinted sensors using chitosan on nitrogen-doped hollow carbon nanospheres for bisphenol A detection.

It is of great significance to establish highly sensitive and reliable detection methods for bisphenol A (BPA) due to its potential health and environmental risks. In this paper, a new type of molecularly imprinted composite sphere was synthesized using nitrogen-doped hollow carbon spheres (N-HCS) as the substrate and chitosan (CS) as the imprinting material. The selective binding ability of this material to BPA and its mechanism were systematically studied.

Benefits and challenges of host depletion methods in profiling the upper and lower respiratory microbiome.

Metagenomic sequencing for respiratory pathogen detection faces two challenges: efficient host DNA depletion and the representativeness of upper respiratory samples for lower tract infections. In this study, we benchmarked seven host depletion methods, including a new method (F_ase), using bronchoalveolar lavage fluid (BALF), oropharyngeal swab (OP), and mock samples. All methods significantly increased microbial reads, species richness, genes richness, and genome coverage while reduced bacterial biomass, introduced contamination, and altered microbial abundance.

Accelerating the exploration of material space for hydride double perovskite superconductors under ambient pressure through machine learning.

In the field of hydride superconductors, a great challenge is to achieve superconducting states under ambient pressure conditions rather than the extreme high-pressure environments that have been required in experiments. Achieving this goal is crucial for advancing the practical applications of high-temperature superconducting materials. We discover a family of compounds (hydride double perovskite superconductors with space group Fm3̄m and chemical formula A2MM'H6) to achieves this goal.

Berbamine promotes autophagy and GPX4 expression through inducing abundant ROS to restrict HIV-1 and Mtb coinfection in macrophages.

Human immunodeficiency virus type 1 (HIV-1) and Mycobacterium tuberculosis (Mtb) co-infection poses a significant public health threat, characterized by a high mortality rate due to impaired host immune responses. In this study, we investigated the role of autophagy, primarily using macrophage cell models co-infected with HIV-1 and Mtb. Our findings indicate that HIV-1 infection or latency significantly suppresses autophagy in macrophages, thereby creating a permissive environment for the survival and replication of intracellular Mtb.

The Preparation of a GO/ZnO/nHAp Composite Coating and the Study of Its Performance Optimization for Pure Titanium Implants.

In this study, a graphene oxide (GO)/zinc oxide (ZnO)/hydroxyapatite (nHAp) composite coating was constructed on a pure titanium surface by microarc oxidation (MAO) pretreatment combined with hydrothermal technology (HT), thereby making it possible to explore the performance optimization of this coating for Ti-based implants. Scanning electron microscopy (SEM), an energy dispersion spectrometer (EDS), Fourier transform infrared spectroscopy (FTIR), Ramam spectroscopy (Ramam), etc., confirmed that the GO/ZnO/nHAp composites were successfully loaded onto the pure Ti surfaces.

Oppositely charged proteins lead to different effects on the bioaccumulation kinetics of polystyrene nanoplastics in zebrafish (Danio rerio).

Nanoplastics (NPs) are ubiquitous in the natural aquatic environment, thus there is a major need to understand their potential health risk toward aquatic organisms. Proteins are widely spread in the water environment, they may interact with NPs and significantly impact the ecotoxicology of NPs in vivo. In this study, positively charged lysozyme (LYZ) and negatively charged ovalbumin (OVA) were selected as model proteins, and their effects on the bioaccumulation kinetics of polystyrene nanoplastics (PS-NPs) in zebrafish were investigated quantitatively using a biokinetic model.

Molecularly imprinted electrochemical sensor based on nitrogen-doped hollow mesoporous carbon spheres with PEDOT for selective and ultrasensitive detection of chlorpromazine.

Molecularly imprinted polymer-based electrochemical sensors (MIP/ECS) can achieve efficient detection of trace pollutants, holding broad application prospects in food safety and environmental monitoring. However, MIP/ECS suffers from weak binding forces at imprinted sites and slow electron transfer rates, which severely limit the selectivity and sensitivity of MIP/ECS. In this study, the synergistic effect of nitrogen-doped hollow mesoporous carbon spheres (N-HCS), polydopamine (PDA) and poly(3,4-ethylenedioxythiophene) (PEDOT) was utilized in the innovative construction of MIP/ECS with excellent selectivity and electrocatalytic activity for the ultrasensitive detection of chlorpromazine.

The peptide from C- Phycocyanin alleviates myocardial ischemia-reperfusion injury by suppressing ferroptosis via upregulating UCHL3.

Myocardial ischemia-reperfusion injury (MIRI) constitutes an essential hurdle following reperfusion therapy for acute myocardial infarction (AMI), which the mechanism involves oxidative stress, inflammatory response, calcium overload, and ferroptosis, etc. MAQAAEYYR (P2), a kind of marine-derived bioactive peptide from C-Phycocyanin (C-PC), exhibits remarkable antioxidant properties. Due to its low molecular weight, P2 exhibits superior bioavailability compared to C-PC.

High-performance supercapacitor based on 3D TiCT electrodes and sulfonated lignin gel electrolyte.

Supercapacitors are highly efficient energy storage systems. The electrode substances and electrolytes, which form their primary components, are key areas of research for scientists. In this study, the transition metal material TiCT and Poly(3,4-ethylenedioxythiophene) (PEDOT)-based polyacrylamide dual-crosslinked network polymers with good conductivity are selected as the cathode material and gel electrolyte, respectively.

Immune modulation: the key to combat SARS-CoV-2 induced myocardial injury.

The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused the Coronavirus disease 2019 (COVID-19) pandemic, has posed significant healthcare challenges. In addition to respiratory complications, it has led to severe damage in other organs, particularly the cardiovascular system. Of which, myocardial injury is increasingly recognized as a most significant complication, contributing to the high mortality.

The transcriptional regulator Sin3a activates CD44 and promotes collective luminal breast cancer cell migration.

Luminal type breast cancer (BrCa) cells invade into the surrounding tissues as collective strands, making them more metastatic than single cells. We have previously reported that the leading subpopulation of collective cells expressed high levels of CD44, which was associated with enhanced migratory and invasive potential of BrCa. It is crucial to elucidate how CD44 becomes enriched in leader cells and contributes to collective migration.

Defect-rich Co,N-doped TiCTx/C/poly (3,4-ethylenedioxy thiophene) molecularly imprinted sensor for ultrasensitive electrochemical detection of gatifloxacin.

The molecular imprinting electrochemical sensors (MIECS) currently face issues such as low sensitivity and stability to be improved. This study innovatively combines ZIF-67/TiCTx, treated at high temperature, with molecular imprinting technology to construct a MIECS with excellent stability and high sensitivity. First, size-controllable ZIF-67 was grown in situ on TiCTx, and after high-temperature treatment, Co,N-TiCTx/C was obtained.

Vγ9Vδ2 T cells expanded with vitamin C combined with HMBPP inhibit intracellular growth.

Background: presents a significant global health threat, and the existing treatments have notable limitations. Vγ9Vδ2 T cells activated by HMBPP can inhibit the growth of intracellular Mtb. Additionally, vitamin C (VC) promotes the differentiation and proliferation of related T cells.

Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT).

Tuberculosis (TB) screening in clinical diagnosis is challenging due to issues such as sputum dependence, time-consuming procedures, and high costs. In this study, we introduce a CRAFT (CRISPR-Driven Aptamer Fluorescence Testing), an aptamer-based CRISPR/Cas12a assay designed for the rapid and sensitive detection of Mycobacterium tuberculosis (Mtb) antigens from peripheral blood. Aptamer 3 (Ap3) and the aptamer-mediated probe (Aptamer-blocker 3-7) were selected through the Systematic Evolution of Ligands by Exponential Enrichment (SELEX).

Pharmacokinetic and tissue distribution analysis of sporoderm-removed Ganoderma lucidum spore powder in rats.

Ganoderma lucidum (Lingzhi), a medicinal mushroom, is recognized for its broad pharmacological activities, including potential health and longevity benefits. Notably, several triterpenoids derived from its spores and fruiting body have demonstrated anti-inflammatory, anti-tumor, and immunomodulatory effects. Despite their therapeutic promise, pharmacokinetic parameters and tissue distribution profiles of these bioactive components are not well characterized, representing a significant knowledge gap.