Multi-frequency electromagnetic radiation promoted hepatic lipid metabolism via the adiponectin pathway in mice.

Radiofrequency electromagnetic radiation (RF-EMR) is ubiquitous in daily life, and the complexity of the electromagnetic environment continues to increase. Nevertheless, research on the biological effects of compound radiofrequency radiation-particularly its influence on lipid metabolism under realistic exposure scenarios-remains limited, and the underlying mechanisms are not yet fully elucidated. To investigate the impact of compound radiofrequency radiation on lipid metabolism, multi-frequency radiation exposure model both normal and obese mice were established.

Case Report: Multidimensional Analgesia via Asymmetric Dual-Target Deep Brain Stimulation of the Periaqueductal Gray and Ventral Posterior Thalamus in Central Post-stroke Pain.

Central post-stroke pain (CPSP) is an intractable neuropathic pain syndrome. Dual-target deep brain stimulation (DBS), which integrates sensory thalamic modulation and endogenous analgesic pathways, has emerged as a potential intervention; however, clinical evidence remains scarce. We report a 54-year-old woman who developed right-sided limb paresthesia progressing to persistent right hemibody pain following a left thalamic hemorrhage.

kmmDE: a Non-parametric Method for Differential Expression Analysis of Time-course RNA-seq Data Using Maximum Mean Discrepancy.

Accurately identifying differentially expressed genes from time-course RNA-Seq data is essential for understanding the dynamics of transcriptome changes during biological processes. Although various methods for differential expression analysis of time-course RNA-seq data were developed, they are limited by their reliance on specific distribution assumptions, and their performance is restricted in datasets without replicates or in short time-series datasets. To address these challenges, we propose a non-parametric method called kmmDE for differential expression analysis based on maximum mean discrepancy.

Dual Inhibition of sEH and COX-2 Improved Cognition in Alzheimer's Disease via Enhanced Myogenic Response and Cerebral Artery Distensibility.

Genetic studies have linked (encoding soluble epoxide hydrolase, sEH) and (encoding cyclooxygenase-2, COX-2) to Alzheimer's disease (AD). Elevated levels of sEH and COX-2 found in AD patients and animals suggest their involvement in neurodegeneration, glial activation, vascular dysfunction, and inflammation. This study evaluated the effects of a new dual sEH/COX-2 inhibitor, PTUPB, on cerebrovascular function and cognition in TgF344-AD rats.

Ajuforrestin A Inhibits Tumor Proliferation and Migration by Targeting the STAT3/FAK Signaling Pathways and VEGFR-2.

Natural products, characterized by their structural novelty, multi-target capabilities, and favorable toxicity profiles, represent a prominent reservoir for the discovery of innovative anticancer therapeutics. In the current investigation, we identified ajuforrestin A, a diterpenoid compound extracted from Maxim, as a potent agent against lung cancer. In vitro, this compound markedly curtailed the proliferation of A549 cells.

A nomogram for predicting post-stroke cognitive impairment no dementia in patients with first-ever mild ischemic stroke.

Objective: To identify significant predictors and construct a validated nomogram for predicting post-stroke cognitive impairment no dementia (PSCIND) risk among first-ever mild ischemic stroke (MIS) patients.

Methods: This retrospective cohort study analyzed 242 first-ever MIS patients categorized into normal cognitive ( = 137) and PSCIND ( = 105) groups. Comprehensive data encompassing demographic characteristics, laboratory parameters, cerebral small vessel disease (CSVD) imaging markers, neuropsychological assessments, and ischemic stroke lesion characteristics were collected.

Initial Insights into the NET-Associated ceRNA Network in Pulpitis: Transcriptomic and Functional Exploration.

Background: Pulpitis is an oral disease primarily triggered by microbial infections. Current therapeutic strategies lack specific agents targeting the underlying pathogenic mechanisms. Non-coding RNAs (ncRNAs) and their competitive endogenous RNA (ceRNA) networks have emerged as critical regulators of diverse biological processes, offering novel insights into the pathogenesis of pulpitis and the identification of potential therapeutic targets.

Causal Association Between Epidermal Growth Factor and Laryngeal/Oropharyngeal Cancer: A 2-Sample Mendelian Randomization Analysis.

The human epidermal growth factor (EGF)-related proteins are thought to play a key role in the pathogenesis of laryngeal cancer (LC) and oropharyngeal cancer (OPC). The aim of this study was to investigate the potential causal relationship between them. A 2-sample Mendelian randomization (MR) analysis utilizing genome-wide association studies (GWAS) data uniquely evaluated causal relationships between EGF-related proteins and both LC and OPC.

Electroacupuncture Exerts Anti-Obesity Effects by Affecting the Gut Microbiota and Upregulating Acetic Acid and 5-HT Levels in Obese Rats.

Background: Obesity is a highly prevalent metabolic disorder that significantly increases the risk of cardiovascular and cerebrovascular diseases. Acupuncture has demonstrated remarkable efficacy in treating obesity, offering substantial societal health benefits.

Objective: Electroacupuncture has shown promise in the treatment of obesity (OB).

Multifunctional Prussian lue nanozymes ameliorate tendinopathy via modulating tissue homeostasis.

Tendinopathy, a prevalent musculoskeletal disorder characterized by chronic pain and functional decline, remains a therapeutic challenge due to the limited efficacy of conventional treatments in addressing oxidative stress and persistent inflammation. Here, we present Prussian blue nanozymes (PBzymes) as a catalytic nanomedicine engineered to mimic multi-enzyme activities, offering a potent strategy for tendon microenvironment modulation and repair. Synthesized via a hydrothermal template-free approach, PBzymes exhibit robust reactive oxygen species (ROS)-scavenging capabilities through intrinsic superoxide dismutase, catalase, and peroxidase-like activities, effectively neutralizing •OH, HO, and •OOH radicals.

Efficacy of adjuvant therapy regimens administered during the perioperative period in resectable non-small cell lung cancer.

Background: We aimed to contribute to the ongoing discussion regarding adjuvant treatment strategies following neoadjuvant chemoimmunotherapy (NCIT) and adjuvant immunotherapy (AIT) after upfront surgery (US) in patients with resectable non-small cell lung cancer (NSCLC).

Methods: 317 patients were retrospectively included from 2019 to 2024. 285 received NCIT, while 32 received AIT after US.

8-epi-Helenalin Suppresses Tumorigenesis and Metastasis by Modulating the STAT3/FAK Signaling Axis.

Hepatocellular carcinoma (HCC) poses a significant global health challenge, highlighting the importance of discovering novel anticancer lead compounds from natural products. In this study, we isolated the sesquiterpene lactone 8-epi-helenalin from the dried flowers of Inula japonica. In vitro experiments demonstrated its potent antiproliferative activity against various tumor cell lines, including human HCC cells (HepG2).

Mechanistic studies on the regulation of ferroptosis by ferritin 1 through the FTH1 signaling pathway affecting oocyte maturation.

Ferrostatin-1 (FER-1) is a ferroptosis inhibitor that blocks lipid peroxidation; however, its role in porcine oocyte maturation remains unclear. This study assessed the effects and regulatory mechanisms of FER-1 on porcine oocyte maturation in vitro by regulating ferroptosis through the ferritin heavy chain 1 (FTH1) signaling pathway. A ferroptosis-induced injury model was established using ammonium ferric citrate (FAC), and a rescue model was established by adding FER-1.

Insights into the chronic toxicity and mechanisms of fluorine-containing pesticides on earthworms.

Fluorine-containing pesticides are widely applied in agriculture, yet their chronic ecotoxic effects on soil organisms remain understudied. This study evaluated the toxicity of three pesticides on Eisenia fetida through a 56-day soil exposure at gradient concentrations (fluxapyroxad:C1:62.5,C2:250, C3:1000 mg a.

Case Report: A case of tuberculous empyema causing rupture of the diaphragm was misdiagnosed as diaphragmatic hernia.

Tuberculous empyema (TE) is a chronic active infection caused by that invades the pleural cavity. Initially, fluid accumulates in the pleural space, followed by an influx of neutrophils, which gradually develops into purulent fluid. This process can eventually lead to pleural thickening and calcification, restricting lung expansion and impairing lung function.

B/T mixed phenotype acute leukemia revealing immunophenotypic lineage-genotype associations and frequent myelodysplasia-related cytogenetic/gene abnormalities: implication for diagnosis and treatment.

B/T mixed-phenotype acute leukemia (MPAL) is a rare subtype of leukemia with diagnostic and therapeutic challenges due to its rarity, genomic diversity, and evolving diagnostic criteria. We report six cases of B/T MPAL with clinicopathological and genomic characterization. Most cases (5/6) demonstrated immunophenotypic/lineage-genotype-associations, i.

Diet, genetic factors, and the risk of gastroesophageal reflux disease, Barrett's esophagus and esophageal adenocarcinoma.

: Diet has been recognized as the most pivotal modifiable lifestyle factor in the development of Gastroesophageal Reflux Disease (GERD), Barrett's Esophagus (BE) and Esophageal Adenocarcinoma (EA). However, the associations between dietary ingredients, patterns, and diet-gene interactions with the risk of GERD, BE and EA remain unclear. : The prospective cohort study included 502 412 participants from the UK Biobank.

Ultra-sensitive and rapid detection of diethylstilbestrol by constructing molecularly imprinted electrochemical sensor via hetero-interface engineering of Pt nanoparticles-decorated MXene/NH2-UiO-66.

The hazard of diethylstilbestrol (DES) towards human health underscored the imperative for highly sensitive and rapid analytical technologies. Herein, a molecularly imprinted electrochemical sensor (MIECS) was innovatively constructed by incorporating the Pt nanoparticles (PtNPs)-decorated MXene/NH-UiO-66 into molecularly imprinted polymer (MIP/MXene/NH-UiO-66/PtNPs) for ultra-sensitive and rapid detection of DES. The introduction of NH-UiO-66 effectively prevented the restacking of MXene and with the synergistic effect of PtNPs, the electrical conductivity of the sensor was significantly enhanced.

Sintilimab plus anlotinib in patients with pre-treated locally advanced or metastatic sarcoma: a prospective, single-arm, phase II clinical trial.

Advanced sarcomas have limited treatment options after standard therapy, and therefore we investigated the efficacy and safety of sintilimab plus anlotinib in this setting. Patients aged 18-75 years with advanced sarcomas and prior systemic therapy were enrolled. Patients with untreated, primary chemotherapy-resistant tumor types, such as alveolar soft part sarcoma, clear cell sarcoma, etc.

MXene-Based Oxygen Electrocatalysts: Mechanistic Insights, Property Tuning Strategies, and Prospects toward Practical Applications.

MXene delivers promising features that are highly compatible with oxygen electrocatalysis, such as excellent electroconductivity, high specific surface area, superhydrophilicity, and tailorable chemically functionalized surfaces, thus being recognized as the ideal platform for developing high-performance catalysts for practical applications in industrial devices. A comprehensive understanding of oxygen catalytic mechanism on MXene ontology and a systematic refining of the general principles toward various physicochemical property regulation strategies are, respectively, the basis and effective alleyway to hitting the target, yet it is currently insufficient and need to be further explored in-depth. Herein, the fundamental effects of MXene on oxygen catalytic activity are sorted out thoroughly, and on this basis, the current mainstream strategies for tuning the property of MXene-based electrocatalysts are classified into four categories, including anion-tuning, cation-tuning, defect/vacancy regulation, and heterometallic dual-site collaboration, where the intrinsic mechanism of each strategy affecting the structure-activity relationship of catalysts is revealed accordingly.

Enhancing the Solubility and Oral Bioavailability of Trimethoprim Through PEG-PLGA Nanoparticles: A Comprehensive Evaluation of In Vitro and In Vivo Performance.

Trimethoprim (TMP), a sulfonamide antibacterial synergist, is widely used in antimicrobial therapy owing to its broad-spectrum activity and clinical efficacy in treating respiratory, urinary tract, and gastrointestinal infections. However, its application is limited due to poor aqueous solubility, a short elimination half-life (t), and low bioavailability. In this study, we proposed TMP loaded by PEG-PLGA polymer nanoparticles (NPs) to increase its efficacy.

Research on highly sensitive detection technology of nitrite based on a novel colorimetric fluorescent probe.

Nitrite (NO), a natural component of the nitrogen cycle, poses significant risks to both ecosystems and human health when present as a pollutant. Hence, it is essential to develop detection methods with high selectivity, sensitivity, and rapid response for measuring nitrite levels in water. This study introduces a novel colorimetric fluorescent probe PQ-N, which specifically recognizes NO in diverse environments including water, soil, and food.

Different Disease Levels Reveal Kiwifruit Brown Spot Impacts on Fruit Yield and Quality.

Kiwifruit brown spot, caused by the fungus , has recently emerged as a problematic foliar disease of kiwifruit, causing premature defoliation. The objective of this study was to determine the effects of kiwifruit brown spot on the yield and quality of kiwifruit. Principal component analysis (PCA) was used to conduct a comprehensive evaluation of the fruit quality of 'Hongyang' kiwifruit in the main producing regions.

Machine Learning-Based Identification and Experimental Validation of Hub Ferroptosis-Related Cuproptosis Genes in Lupus Nephritis.

Background: The role of ferroptosis and cuproptosis in lupus nephritis (LN) is unclear. The aim of this study was to explore the expression and effects of ferroptosis-related cuproptosis genes (FRCGs) in LN using bioinformatics and experimental validation.

Methods: The LN-related datasets GSE112943 and GSE32591 were downloaded from the GEO database.