kmmDE: a Non-parametric Method for Differential Expression Analysis of Time-course RNA-seq Data Using Maximum Mean Discrepancy.

Accurately identifying differentially expressed genes from time-course RNA-Seq data is essential for understanding the dynamics of transcriptome changes during biological processes. Although various methods for differential expression analysis of time-course RNA-seq data were developed, they are limited by their reliance on specific distribution assumptions, and their performance is restricted in datasets without replicates or in short time-series datasets. To address these challenges, we propose a non-parametric method called kmmDE for differential expression analysis based on maximum mean discrepancy.

Challenging 21-day quarantine guidelines for mumps in Beijing: Data-driven recommendations for a high-coverage MMR vaccination era (2019-2024).

Mumps is a vaccine-preventable disease. Following Beijing's implementation of a three-dose MMR vaccination strategy, incidence rates declined significantly. However, the current 21-d quarantine period lacks evidence-based support, resulting in low compliance and conflicts between healthcare providers and patients.

Motion-Robust Direct Myelin Imaging in MRI using Self-Gating.

Direct myelin imaging with inversion-recovery ultrashort-echo-time (IR-UTE) is highly motion-sensitive, yet extra hardware or longer scans are impractical. We evaluated whether a superior-inferior (SI) self-navigator with bit-reversed spoke-angles mitigates motion artifacts without extending acquisition. Dual-echo IR-UTE was implemented at 3T.

Shifts in Epidemiology and Antibiotic Resistance of Haemophilus Influenzae in Children From Chongqing (2019-2024): Pre-, During, and Post-COVID-19.

Objective: To comprehensively investigate the epidemiology and analyze the antimicrobial resistance of Haemophilus influenzae isolated from children in Chongqing before, during, and after the COVID-19 pandemic.

Methods: A total of 21,723 Haemophilus influenzae strains from four Chongqing hospitals during 2019-2024 were included. Antimicrobial susceptibility testing was conducted according to Clinical and Laboratory Standards Institute 2024 breakpoints using Kirby-Bauer method or automated systems.

Small extracellular vesicles derived from hair follicle neural crest stem cells enhance perineurial cell proliferation and migration via the TGF-β/SMAD/HAS2 pathway.

Peripheral nerve defect repair is a complex process that involves multiple cell types; perineurial cells play a pivotal role. Hair follicle neural crest stem cells promote perineurial cell proliferation and migration via paracrine signaling; however, their clinical applications are limited by potential risks such as tumorigenesis and xenogeneic immune rejection, which are similar to the risks associated with other stem cell transplantations. The present study therefore focuses on small extracellular vesicles derived from hair follicle neural crest stem cells, which preserve the bioactive properties of the parent cells while avoiding the transplantation-associated risks.

Epigenomic Profiling Positions ATF7 as a Core Regulator of Colonic Inflammation.

Mitochondrial dysfunction plays a central role in epithelial damage and persistent inflammation in ulcerative colitis (UC), but the transcriptional mechanisms that govern mitochondrial quality control in the intestinal epithelium remain poorly defined. Here, we identify Activating Transcription Factor 7 (ATF7) as a key regulator of mitophagy in colonic epithelial cells. Integrative transcriptomic and epigenomic analyses of patient-derived mucosal samples revealed marked ATF7 downregulation and widespread activation of inflammatory pathways.

5-(4-Hydroxy-1,3-dioxoisoindolin-2-yl)-2,3-dihydrophthalazine-1,4-dione (HDDD) as effective substrate in HRP-catalyzed enhanced chemiluminescence reaction.

By modifying the amino group of luminol, 5-(4-hydroxy-1,3-dioxoisoindolin-2-yl)-2,3-dihydrophthalazine-1,4-dione (HDDD) was synthesized. Using a combination of full factorial design and "one-variable-time" method, favorable conditions for the generation of HDDD-dependent chemiluminescence were determined. It was shown that replacing luminol with HDDD in the HRP-C-catalyzed enhanced chemiluminescence reaction increased the luminescence intensity.

Development of a validated and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to simultaneous determination of crizotinib, alectinib and lorlatinib in human plasma.

Crizotinib, alectinib, and lorlatinib are tyrosine kinase inhibitors used sequentially in non-small cell lung cancer (NSCLC) therapy. Therapeutic drug monitoring (TDM) is valuable during sequential treatment, enabling simultaneous quantification. Consequently, an analytical method capable of multiplexing these three compounds into a single assay is highly applicable for routine TDM.

Glutamate-associated research in Parkinson's disease: a bibliometric analysis.

Background: Parkinson's disease (PD) is a common neurodegenerative disease. As an important excitatory neurotransmitter, glutamate plays a key role in the nervous system. The imbalance of glutamate levels, the dysfunction of related receptors, and the abnormal activity of transporters are closely associated with the pathological process of PD.

Triptolide improves microbial dysbiosis and metabolite disorder in db/db mice.

Background: Diabetic kidney disease (DKD) is an increasing global public health problem. Triptolide (TP) has a good therapeutic effect on DKD and is widely used in China. However, the mechanism of TP is still unclear.

Branched-chain amino acids induce hyperammonemia via gut-liver axis-mediated ammonia overproduction in laying hens.

Gut microbiota not only biosynthesizes branched-chain amino acids (BCAA) but also catabolizes and utilizes them, while the effects of dietary BCAA supplementation on intestinal microbiota and metabolism remain largely elusive. Therefore, the present study aimed to investigate the impacts of dietary BCAA supplementation on productive performance, egg quality, gut microbiota and metabolism in laying hens. A total of 180 Fengda No.

Characteristics and Related Factors of Chinese Herbal Medicine Use in Middle-Aged and Older Patients with Cardiovascular Disease in China: A Cross-Sectional Study.

Background: Cardiovascular disease (CVD) poses a significant health burden in middle-aged and older Chinese populations, with Chinese herbal medicine (CHM) often used as a complementary therapy. This study aimed to analyze the characteristics of CHM use and its influencing factors in middle-aged and older Chinese patients with CVD.

Methods: This was a cross-sectional study based on data from the 2018 China Health and Aging Longitudinal Study (CHARLS).

AtCHE1, the Arabidopsis homolog of mammalian AATF/Che-1 protein, is involved in safeguarding genome stability.

Both endogenous and exogenous genotoxins can inflict damage on cellular DNA, leading to reduced genomic stability in plants, which adversely affects development. Apoptosis Antagonizing Transcription Factor (AATF), also referred to as Che-1, has been identified as a binding protein for RNA polymerase II. It plays a crucial role in various cellular processes, including cell proliferation, transcriptional regulation, apoptosis, DNA damage response, and ribosome biogenesis in mammals.

Anterior Chamber Angle Changes and Its Associated Factor After Posterior Chamber Phakic Intraocular Lens Implantation in Eyes With Shallow Anterior Chambers.

Purpose: The purpose of this study was to investigate anterior chamber angle (ACA) changes after implantable collamer lens (ICL) placement in eyes with shallow anterior chamber depth (ACD) and the effect of vault size on ACA changes.

Methods: In this prospective cohort study of 120 patients (120 eyes) undergoing ICL implantation, participants were stratified into shallow (ACD ≤3.0 mm, n = 60) and normal ACD groups (ACD >3.

The effect of high approach-motivated positive affect on selective attention under high perceptual load.

Introduction: Selective attention is a crucial mechanism that enables humans to navigate complex environments and accomplish targeted tasks, garnering significant interest from researchers. Numerous studies have found that selective attention can be influenced by emotions; however, previous research has primarily focused on the effects of valence and arousal, neglecting the role of motivation, another dimension of emotion. Additionally, it remains unclear whether emotional motivation's influence on selective attention differs across various levels of perceptual load.

Genome-Wide Analysis and Screening of Uridine Diphosphate-Glycosyltransferase Family Genes Involved in Lignin/Flavonoid Glycosylation and Stress Response in (L.) Gaudich.

Lignins and flavonoids, which are derived from the phenylpropanoid pathway and share common precursors, play an important role in (ramie). Uridine diphosphate-glycosyltransferases (UGTs) are essential for the glycosylation of secondary metabolites and are involved in plant growth and stress responses. Hence, this study aimed to screen candidate related to lignin/flavonoid glycosylation and stress responses.

Pathogenicity of spp. Against : Effects on Immune Enzyme Activities and Gene Expression Profile.

(1) Background: spp. are fungi known for producing cyclosporin A and their ability to infect insects. However, their pathogenicity against the lepidopteran pest has not been previously reported.

Transcriptomic Identification of Key Genes Responding to High Heat Stress in Moso Bamboo ().

Moso bamboo (), the most widely distributed bamboo species in China, is valued for both its shoots and timber. This species often faces challenges from high-temperature stress. To cope with this stress, Moso bamboo has evolved various adaptive mechanisms at the physiological and molecular levels.

Optimising Regimen of Co-Amoxiclav (ORCA)-The Safety and Efficacy of Intravenous Co-Amoxiclav at Higher Dosing Frequency in Patients with Diabetic Foot Infection.

: With increasing pharmacokinetic evidence suggesting the inadequacy of conventional dose intravenous co-amoxiclav (IVCA) 1.2 g Q8H in targeting , our institution antibiotic guidelines optimised dosing recommendations for diabetic foot infection (DFI) management to 1.2 g Q6H in August 2023.

Transcriptome Analysis Reveals the Molecular Mechanisms by Which Influences the Proliferation of Ovarian Granulosa Cells in Sheep.

Normal proliferation of ovarian granulosa cells is essential for follicular development. The results of this study showed that ADAMTS1 was primarily localized in the cytoplasm of granulosa cells in sheep ovarian follicles, as revealed by immunohistochemistry and immunofluorescence staining. Knockdown and overexpression experiments of in granulosa cells demonstrated that the number of EdU-positive cells significantly decreased in the knockdown group ( < 0.

Relationship between symptom burden and benefit finding in patients with gastrointestinal cancers undergoing chemotherapy: the chain-mediating role of comprehensive needs and communication pattern.

Background: Patients with gastrointestinal (GI) cancer undergoing chemotherapy frequently exhibit heterogeneous symptom burden, while psychological adaptation may exert differential impacts on benefit finding.

Objectives: To explore the relationship between symptom burden and benefit finding in GI cancer patients, and to determine whether comprehensive needs and communication pattern play a mediating role in this relationship.

Methods: This was a cross-sectional study.

The STUB1-TPIT axis regulates the secretion of adrenocorticotrophic hormone in cushing disease.

Background: Cushing's disease (CD) is a clinical syndrome caused by excessive secretion of adrenocorticotropic hormone (ACTH) from a pituitary corticotroph adenoma, resulting in adrenal cortical hyperplasia and overproduction of cortisol. The T-box transcription factor (TPIT) is crucial for regulating ACTH secretion in pituitary corticotroph adenomas. This study aims to explore the ubiquitin-mediated degradation of TPIT and identify potential pharmaceutical agents for treating CD.

Neck-to-height ratio in screening for abdominal obesity among children and adolescents: a cross-sectional study.

Background: With the increasing prevalence of abdominal obesity among children and adolescents, identifying easily measurable, safe, efficient, low-cost, and highly effective indicators is crucial in public health management. While neck-to-height ratio (NHtR) is a better indicator of upper body fat than neck circumference (NC), current research on this indicator mainly assesses its role in obesity and related metabolic diseases among adults. Therefore, this study aimed to investigate the clinical significance of the (NHtR) in screening for abdominal obesity among children and adolescents.

KLF9 in cancer: a potential prognostic marker and therapeutic target.

Kruppel-like factor 9 (KLF9) is a transcription factor that has gained significant attention in recent years for its critical involvement in development and progression of various cancers. Recent research has revealed the dual nature in tumorigenesis, where KLF9 can function as either a tumor suppressor or an oncogene, depending on the cellular context. Clinically, KLF9 emerges as a potential prognostic biomarker due to its differential expression patterns across various cancer types, with lower KLF9 levels often correlating with poorer patient outcomes.

On-chip Cherenkov radiation tuning in 3.2-14 THz.

Cherenkov radiation has attracted much attention for promoting the free electron radiation sources. Using hyperbolic metamaterial, Cherenkov radiation can be excited using low-energy electrons and thus on-chip free electron radiation source has been realized. However, direct experimental observations of on-chip free-electron-based Cherenkov radiation have been limited to the visible region, and the tunability has not been thoroughly explored.