Screening efficacy of erythroferrone and hepcidin in identifying thalassemia with iron-deficiency anemia among pregnant women.

This study investigated the prevalence of Thalassemia (Thal) gene in pregnant women in the Quanzhou area, China. And explored the clinical application value of Erythroferrone (ERFE) and Hepcidin in screening pregnant women with Thal complicated by Iron-deficiency anemia (IDA, defined as serum ferritin (SF) < 20 μg/L). From January 2020 to December 2022, the detection rate of Thal in suspected Thal populations was 35.

Characteristics and Related Factors of Chinese Herbal Medicine Use in Middle-Aged and Older Patients with Cardiovascular Disease in China: A Cross-Sectional Study.

Background: Cardiovascular disease (CVD) poses a significant health burden in middle-aged and older Chinese populations, with Chinese herbal medicine (CHM) often used as a complementary therapy. This study aimed to analyze the characteristics of CHM use and its influencing factors in middle-aged and older Chinese patients with CVD.

Methods: This was a cross-sectional study based on data from the 2018 China Health and Aging Longitudinal Study (CHARLS).

Whole exome sequencing analysis of susceptibility loci in transgender individuals.

Background: Genetic factors contributing to sex-associated dimorphic brain development may also underlie gender identity-related anxiety disorders.

Aim: To establish a high-throughput whole-exome sequencing (WES) and bioinformatics pipeline for identifying rare variants in sex-dimorphic neural pathways and explore their association with gender identity-related anxiety.

Methods: Peripheral genomic DNA was collected from 23 patients (13 Assigned male at birth (AMAB), 10 Assigned female at birth (AFAB)) presenting with gender identity-related anxiety at Shanghai Mental Health Centre between March 2020 and February 2022.

Impact of tris (2-chloropropyl) phosphate and triphenyl phosphate on honeybee (Apis mellifera L.) midgut health: Assessing toxicity and potential mitigation with melatonin.

Organic phosphorus flame retardants (OPFRs) are persistent environmental pollutants known for their mammalian toxicity, yet their risks to pollinators, particularly honeybees, have not been extensively studied. This study investigates the toxicological impacts of two representative OPFRs, tris(2-chloropropyl) phosphate (TCPP) and triphenyl phosphate (TPHP), on honeybees (Apis mellifera L.) and examines potential protective strategies.

WTAP participates in the DNA damage response via an mA-FOXM1-dependent manner in hepatocellular carcinoma.

N6-Methyladenosine (mA) is one of the most abundant RNA modifications that occur in eukaryotes. The relationship between mA methylation and DNA damage repair (DDR) is still unclear. As an important chaperone protein of mA methyltransferase, the role of Wilm's tumor-associated protein (WTAP) in DDR has not been studied in detail.

High-Resolution Spatial Transcriptomics Unveils Spatially Resolved Gene Modules and Fatty Acid Metabolism Dysregulation in Human Skin Aging.

Human skin aging involves intricate micrometer-scale changes in cellular organization. High-resolution spatial transcriptomic analysis is crucial for deciphering the complex interplay of cell types and molecular processes underlying skin aging. Here, the Stereo-seq technique was employed to generate a high-resolution spatial transcriptomic atlas of human eyelid skin, capturing age-related alterations in gene expression.

Disturbed engram network caused by NPTX downregulation underlies aging-related contextual fear memory deficits.

Engram cells storing episodic memories are allocated to separate neuronal ensembles. However, how these ensembles maintain their stability to drive precise memory expression, and whether their destabilization contributes to aging-related memory deficits, remain elusive. Here, we show that during contextual fear memory consolidation, neuronal pentraxin 1 (NPTX1) in Fos transcription-dependent ensemble (F-RAM) of the dentate gyrus (DG) promotes memory expression in the fear context.

A Dual-Functional NIR Fluorescent Probe for Detection SO Derivatives and Colorimetric Sensing pH as well as its Applications in Food Monitoring and Hepatoma Cells Imaging.

Abnormal SO derivative levels can induce mitochondrial dysfunction, which is associated with liver cancer disease. The pH levels is also a significance parameter in the process of food spoilage. It is evident that developing a detection tool for SO derivatives in biological systems and pH fluctuations in food samples is of great significance.

Functional Analysis of miR-148a: A Differentially Expressed microRNA in Hemifacial Microsomia.

Introduction: Hemifacial Microsomia (HFM) is the second most common congenital deformity, yet its etiology and pathogenesis remain unclear. Therefore, this study aimed to identify differentially expressed microRNAs (miRNAs) between healthy and affected bone marrow mesenchymal stem cells (BMSCs) from HFM patients, focusing on the functional roles of miR-148a in osteogenesis and osteoclastogenesis.

Methods: The specific expression of microRNAs was screened by sequencing and verified by PCR.

Prenatal diagnosis and genetic assessment of fetuses with single umbilical artery using chromosomal microarray analysis: a seven-year single-center retrospective study.

Background: The inherited causes behind fetuses with a single umbilical artery (SUA) are still poorly understood, largely because published studies are scarce. In the present research, efforts were made to uncover the genetic factors at play and to assess how SUA influences pregnancy outcome.

Methods: A retrospective review was performed on 5,014 pregnant individuals who underwent prenatal diagnostic testing between September 2017 and April 2023.

Impact of Di-(2-Ethylhexyl)-Phthalate on Metabolic Syndrome: Insights from Network Toxicology and Molecular Docking and Dynamics.

Background: Metabolic syndrome (MetS) is strongly associated with exposure to environmental pollutants, especially endocrine disruptors (EDCs). Di-(2-ethylhexyl)-Phthalate (DEHP), a typical EDC widely found in plastic products, has been shown to interfere with lipid metabolism and insulin signalling. However, the specific molecular mechanism by which it mediates MetS remains unclear.

Brain functional connectivity associated with cognitive deficits in younger patients at first episode of schizophrenia.

Background: Cognitive deficits are a core feature of schizophrenia and are influenced by the age at onset of psychosis. Both preclinical and early-onset schizophrenia patients exhibit abnormalities in the frontal cortex, parahippocampus, precuneus, and hippocampus-regions that are closely associated with cognitive deficits. This study examines whether cognitive-related functional connectivity (FC) within these regions differs in first-episode schizophrenia (FES) patients with varying ages of onset.

Design, synthesis, and biological evaluation of novel PI3Kδ/HDAC6 dual inhibitors for the treatment of non-Hodgkin's lymphoma.

PI3Kδ and HDAC6 inhibitors play important roles in the treatment of lymphoma. Herein, a novel series of purine-based PI3Kδ/HDAC6 dual inhibitors have been rationally designed and synthesized by incorporating an HDAC pharmacophore into our previously reported PI3Kδ inhibitor scaffold. Structure-activity relationship (SAR) studies led to the discovery of the lead compound 22E, which showed potent inhibitory activity towards PI3Kδ and HDAC6, with IC values of 2.

Cytoplasmic Histone Is a Marker of Pro-Inflammatory CD4 T Cells.

Although extranuclear histones have been identified for a long time, their role in lymphocytes, particularly cytoplasmic histones, remains elusive. In this study, we conducted a visual and quantitative analysis of cytoplasmic histones in CD4 T cells and discovered that effector CD4 T cells contain higher levels of cytoplasmic histones compared to naïve T cells. We observed a significant increase in cytoplasmic histones following T cell receptor (TCR) activation, with further elevation during the differentiation of Th1 and Th17 cells.

Natural and long-term preservable anticoagulant property of SiO and TiO bilayer films.

Introduction: Titanium dioxide (TiO2) films have been widely studied as blood-contacting materials, but their positively charged surface and low density of surface hydroxyl (-OH) groups result in poor intrinsic anticoagulant properties. Furthermore, TiO surfaces readily adsorb carbon-containing contaminants from the environment, causing a rapid decline in anticoagulant performance during storage. Thus, improving TiO's intrinsic anticoagulant properties and extending its shelf-life remain challenging.

CCl-Induced Synthesis of Phosphonothioates, Sulfoxides, and Sulfones from Sodium Sulfinates.

An external reductant-free and acid-free synthesis of phosphonothioates from odorless sodium sulfinates induced by CCl (1.0 equiv) under mild conditions has been achieved. This transformation provides an efficient synthetic approach to phosphonothioates in up to 98% yield with good functional group compatibility.

Dual Exosome Coating for Modulating Endothelial Function and Inflammation.

Conventional drug-eluting stents can lead to complications such as in-stent restenosis and late thrombosis due to the lack of a well-functioning endothelium and inadequate inflammatory regulation. In this study, a dual exosome coating was fabricated to promote endothelial function and macrophage efferocytosis via a synergistic effect by endothelial cell-sourced and mesenchymal stem cell-sourced exosomes. By the biotin-avidin interaction, the dual exosomes were proportionally grafted onto the substrate and evenly distributed.

Research on the online service mechanism of internet hospital in infectious disease prevention and control.

Infectious diseases can sometimes lead to pandemics, often transmitted through public and social gatherings, including in-person hospital visits. Consequently, there is an urgent need for innovative approaches to prevent their spread. Taking COVID-19 as an example, we have explored a remote, contactless hospital online model that offers the public online medical consultations, professional psychological counseling, and chronic disease management consultations, thereby mitigating the risk of new transmissions resulting from hospital visits.

Combined exposure to atrazine and phoxim exacerbated the alterations of enzyme activity and abnormal gene expression in earthworms (Eisenia fetida).

Multiple pesticides often coexist in soil, potentially leading to interactions among their components, these may detrimentally impact soil organisms. This study assessed the potential risks posed by simultaneous exposure to atrazine (ATR) and phoxim (PHO) on enzyme and transcription levels in earthworms (Eisenia fetida). The results revealed that ATR exhibited higher acute toxicity towards E.

Glycemic control and prostate antigen levels in individuals with diabetes based on NHANES data.

The relationship between diabetes and prostate-specific antigen (PSA) levels is complex, with potential implications for prostate cancer screening. This study examined the association between glycemic control and total PSA (tPSA) levels in patients with diabetes. We analyzed data from the 2001-2010 NHANES to assess the relationship between glycated hemoglobin (HbA1c) and tPSA in adults with diabetes, categorizing HbA1c as < 7% (good glycemic control) or ≥ 7% (poor glycemic control).

A Surgical Technique of Inner Capsule Vaporization With CO2 Laser for Vocal Fold Cysts.

Objectives: Traditional surgical methods for vocal cysts, such as enucleation and marsupialization, present inherent limitations, including the risk of cyst rupture and extensive tissue damage. Our aim was to introduce a new surgical approach for treating mucous retention cysts while ensuring the preservation of intact mucosa, thus promoting rapid recovery.

Methods: In this retrospective study, 15 patients diagnosed with mucous retention cysts underwent the novel surgery procedure, which involved incising the cyst capsule, vaporizing the inner capsule using a CO2 laser, and repositioning the mucosal tissue.

Evaluating the combined toxicity of broflanilide and myclobutanil on honeybees (Apis mellifera L.): Molecular mechanisms and protective effects of curcumin.

Pesticide toxicity to honeybees has become a pressing ecological issue, yet the effects of pesticide co-exposure are still not fully understood. This research investigates the toxicological implications of concurrent exposure to broflanilide (BFL), a novel diamide insecticide, and myclobutanil (MYC), a commonly used triazole fungicide, on honeybees (Apis mellifera L.), while exploring potential preventive strategies.